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The map label for this gene is spoIIE [H]

Identifier: 52783921

GI number: 52783921

Start: 75115

End: 77604

Strand: Direct

Name: spoIIE [H]

Synonym: BLi00080

Alternate gene names: 52783921

Gene position: 75115-77604 (Clockwise)

Preceding gene: 52783920

Following gene: 52783922

Centisome position: 1.78

GC content: 45.94

Gene sequence:

>2490_bases
ATGGAAAAAGCGGAAAGAAGAGTGAACAGCCCAATAGCTGGACCTGCTGTTCAAAAATTGTATTCATGGTTTGGCAGCAT
GACGAAGCTTATGATGCAGCATTTATATTCCCTCTTTTTTTATAAAGGGCTGATCTATATGGTCATCGGTTTTTTATTGG
GACGAGCCTTCATTCTGTCAGAGGTCATTCCCTTTGCTCTTCCATTTTTCGGAGCGATGCTTTTAATCAAAAAAGATAAA
GCTTTCCTTGCATGCCTGGCGCTTCTTGCCGGAGCTCTGAGCATATCGCCTCAGCATTCGCTGTTCGTGCTTGCGGCTCT
GTTTGCATTTGCGATATGTTCAAAAATGACGTCCCTTATTATAAAAGACCGTGTCAGAACGCTGCCTGTCGTCGTCTTTT
TGGCGATGGCTGTGACAAGATGCGGATTCGTATATGCCGAATACGGAACGGTTTCAGGTTATCACTATATTATGGCTTTC
GTTGAAGCCGGATTATCGTTTATCCTCACATTGATTTTTCTGCAAAGCCTGCCGATTGTCACATCAAAGCGGGCGAAACA
GTCGCTGAAAATTGAAGAGATCATTTGTTTTATGATTTTAATCGCTTCCGTTCTCACGGGCTTGACAGGCGTTTCATTTC
AAGGCATGCAGGCTGAACTGATATTGGCCCGTTATGTCGTGCTCGCTTTCGCGTTCATCGGAGGCGCAAGCATCGGCTGT
ACAGTCGGGGTTGTGACCGGGCTGATTCTCAGCCTCTCAAACATCGGCAATTTATATCAGATGAGCCTGCTGGCTTTCTC
AGGCCTTCTCGGCGGTTTGCTAAAAGAAGGAAAAAAGTTCGGCGCAGCGGTCGGCTTATTGATTGGATCTCTATTGATTT
CTCTGTACGGAGAAGGTTCGGCTGAATTAGTGCCGACGCTTTATGAATCTCTGATTGCAATCGGCCTGTTCCTTTTAACC
CCTCAGTCGATTACGAAAAAAGTGGCCAAGTATATACCTGGAACGACTGAGCACGCCCAGGAACAGCAGCAGTATGCAAG
GAAAATCCGCGATGTCACCGCCCAAAAAGTCGATCAGTTTTCGAACGTTTTTCACGCTTTATCCGAAAGCTTCGCTACCT
TTTATCATTCAGCTCCGGACGATGAAGGAAAAGAAAAAGAGATCGATCTGTTTTTGAGCACGGTGACAGAACATTCCTGT
CAGTCATGCTATAAGAAAAACAAGTGCTGGGTTCAGAACTTTGATAAAACATATGATTTGATGAAACGGGTTATGCAGGA
AACGGAAGAAAAGCAATATTTTAAAAACCGCAAGCTGAAAAAGGAGTTTCATCAGCACTGCTCCAAATCAAAGCAAGTAG
AAGCGCTGATTGAAGACGAGCTGACTCATTTTAGGGCGAACCAGACATTAAAACAAAAGGTGCATGACAGCAGACGTCTC
GTTGCAGAGCAGCTTCTCGGCGTTTCTCAGGTTATGGCGGACTTTTCTCGGGAAATAAAAAGGGAAAGGGAGCAGCATTT
TATTCAAGAAGAGCAAATTCGGGATGCGCTGCAGCACTTCGGCATCGAGATTCAGCAAGTCGAAATATACAGCCTTGAGC
AGGGAAACATCGATATTGAAATGAGTATCCCGTATTGCAACGGCCATGGAGAGTGTGAAAAAATCATCGCTCCGATGCTG
TCCGATATTTTGGAAGAACAAATTATCGTCAAAGCAGAACAGTGCGCCGGCCATCCGAATGGATATTGTCATGTTGCCTT
CGGTTCGGCGAAGTCATACAGGGTGGTGACAGGAGCCGCGCATGCAGCAAAAGGCGGAGGGCTTGTCTCCGGCGACAGCT
ACAATATGATGGAGCTCGGCACCGGCAAATATGCCGCCGCCATTAGCGATGGTATGGGAAATGGCGCAAGGGCCCATTTT
GAAAGCAATGAGACGATCAAGCTGCTGGAAAAGATTCTTCAGTCGGGCATCGACGAAAAAGTGGCGATTAAAACGATTAA
CAGCATTCTTTCATTAAGGACAACAGATGAAATTTATTCGACATTGGATTTATCGGTCATCGATCTTCAGGATGCGAGCT
GCAAGTTTTTGAAAATCGGCTCCACCCCGAGCTTTATTAAAAGAGGCGATCAAATTATAAAAGTGCAGGCCAGCAATCTG
CCGATCGGCATCATTACAGAATTCGATGTCGATGTTGTCAGCGAGCAATTAAAAGCGGGAGACCTTTTGATCATGATGAG
CGACGGAATCTTTGAAGGGCCGAGACATGTGGAAAATCATGATCTGTGGATGAAGCGCAAATTGAAATCGCTGAAAACCG
AGGAGCCGCAGGAAATCGCCGACTTAATCATGGAAGAAGTGATCCGGACAAGGTCGGGTCTGATTGAGGACGACATGACG
GTGATTGTCATCAAGCTGGACCATAATACGCCAAAGTGGGCCTCCATTCCGGCGCCGGCTTTTTTCCAAAAGAATCAAGA
GATTTCTTAG

Upstream 100 bases:

>100_bases
GTCGAACAGTTCTTTGAATTCGTTACCTTCTTTTGACAAAATCCTATTTCATCTTTCGCTATAATGGCAAGCAACGAATA
AACGAGTGGGAGATGAGAGC

Downstream 100 bases:

>100_bases
CATTCGTATAAATCAAATTTCTTCTGGCGATGATGGAACTAAATCAAGATATCTTTGTCCAGGAGGAATAAAAACGATGA
AAAAGGGGCATTTGAATCAA

Product: SpoIIE

Products: NA

Alternate protein names: Stage II sporulation protein H [H]

Number of amino acids: Translated: 829; Mature: 829

Protein sequence:

>829_residues
MEKAERRVNSPIAGPAVQKLYSWFGSMTKLMMQHLYSLFFYKGLIYMVIGFLLGRAFILSEVIPFALPFFGAMLLIKKDK
AFLACLALLAGALSISPQHSLFVLAALFAFAICSKMTSLIIKDRVRTLPVVVFLAMAVTRCGFVYAEYGTVSGYHYIMAF
VEAGLSFILTLIFLQSLPIVTSKRAKQSLKIEEIICFMILIASVLTGLTGVSFQGMQAELILARYVVLAFAFIGGASIGC
TVGVVTGLILSLSNIGNLYQMSLLAFSGLLGGLLKEGKKFGAAVGLLIGSLLISLYGEGSAELVPTLYESLIAIGLFLLT
PQSITKKVAKYIPGTTEHAQEQQQYARKIRDVTAQKVDQFSNVFHALSESFATFYHSAPDDEGKEKEIDLFLSTVTEHSC
QSCYKKNKCWVQNFDKTYDLMKRVMQETEEKQYFKNRKLKKEFHQHCSKSKQVEALIEDELTHFRANQTLKQKVHDSRRL
VAEQLLGVSQVMADFSREIKREREQHFIQEEQIRDALQHFGIEIQQVEIYSLEQGNIDIEMSIPYCNGHGECEKIIAPML
SDILEEQIIVKAEQCAGHPNGYCHVAFGSAKSYRVVTGAAHAAKGGGLVSGDSYNMMELGTGKYAAAISDGMGNGARAHF
ESNETIKLLEKILQSGIDEKVAIKTINSILSLRTTDEIYSTLDLSVIDLQDASCKFLKIGSTPSFIKRGDQIIKVQASNL
PIGIITEFDVDVVSEQLKAGDLLIMMSDGIFEGPRHVENHDLWMKRKLKSLKTEEPQEIADLIMEEVIRTRSGLIEDDMT
VIVIKLDHNTPKWASIPAPAFFQKNQEIS

Sequences:

>Translated_829_residues
MEKAERRVNSPIAGPAVQKLYSWFGSMTKLMMQHLYSLFFYKGLIYMVIGFLLGRAFILSEVIPFALPFFGAMLLIKKDK
AFLACLALLAGALSISPQHSLFVLAALFAFAICSKMTSLIIKDRVRTLPVVVFLAMAVTRCGFVYAEYGTVSGYHYIMAF
VEAGLSFILTLIFLQSLPIVTSKRAKQSLKIEEIICFMILIASVLTGLTGVSFQGMQAELILARYVVLAFAFIGGASIGC
TVGVVTGLILSLSNIGNLYQMSLLAFSGLLGGLLKEGKKFGAAVGLLIGSLLISLYGEGSAELVPTLYESLIAIGLFLLT
PQSITKKVAKYIPGTTEHAQEQQQYARKIRDVTAQKVDQFSNVFHALSESFATFYHSAPDDEGKEKEIDLFLSTVTEHSC
QSCYKKNKCWVQNFDKTYDLMKRVMQETEEKQYFKNRKLKKEFHQHCSKSKQVEALIEDELTHFRANQTLKQKVHDSRRL
VAEQLLGVSQVMADFSREIKREREQHFIQEEQIRDALQHFGIEIQQVEIYSLEQGNIDIEMSIPYCNGHGECEKIIAPML
SDILEEQIIVKAEQCAGHPNGYCHVAFGSAKSYRVVTGAAHAAKGGGLVSGDSYNMMELGTGKYAAAISDGMGNGARAHF
ESNETIKLLEKILQSGIDEKVAIKTINSILSLRTTDEIYSTLDLSVIDLQDASCKFLKIGSTPSFIKRGDQIIKVQASNL
PIGIITEFDVDVVSEQLKAGDLLIMMSDGIFEGPRHVENHDLWMKRKLKSLKTEEPQEIADLIMEEVIRTRSGLIEDDMT
VIVIKLDHNTPKWASIPAPAFFQKNQEIS
>Mature_829_residues
MEKAERRVNSPIAGPAVQKLYSWFGSMTKLMMQHLYSLFFYKGLIYMVIGFLLGRAFILSEVIPFALPFFGAMLLIKKDK
AFLACLALLAGALSISPQHSLFVLAALFAFAICSKMTSLIIKDRVRTLPVVVFLAMAVTRCGFVYAEYGTVSGYHYIMAF
VEAGLSFILTLIFLQSLPIVTSKRAKQSLKIEEIICFMILIASVLTGLTGVSFQGMQAELILARYVVLAFAFIGGASIGC
TVGVVTGLILSLSNIGNLYQMSLLAFSGLLGGLLKEGKKFGAAVGLLIGSLLISLYGEGSAELVPTLYESLIAIGLFLLT
PQSITKKVAKYIPGTTEHAQEQQQYARKIRDVTAQKVDQFSNVFHALSESFATFYHSAPDDEGKEKEIDLFLSTVTEHSC
QSCYKKNKCWVQNFDKTYDLMKRVMQETEEKQYFKNRKLKKEFHQHCSKSKQVEALIEDELTHFRANQTLKQKVHDSRRL
VAEQLLGVSQVMADFSREIKREREQHFIQEEQIRDALQHFGIEIQQVEIYSLEQGNIDIEMSIPYCNGHGECEKIIAPML
SDILEEQIIVKAEQCAGHPNGYCHVAFGSAKSYRVVTGAAHAAKGGGLVSGDSYNMMELGTGKYAAAISDGMGNGARAHF
ESNETIKLLEKILQSGIDEKVAIKTINSILSLRTTDEIYSTLDLSVIDLQDASCKFLKIGSTPSFIKRGDQIIKVQASNL
PIGIITEFDVDVVSEQLKAGDLLIMMSDGIFEGPRHVENHDLWMKRKLKSLKTEEPQEIADLIMEEVIRTRSGLIEDDMT
VIVIKLDHNTPKWASIPAPAFFQKNQEIS

Specific function: Normally needed for pro-sigma E processing during sporulation but can be bypassed in vegetative cells. Activates spoIIAA by dephosphorylation [H]

COG id: COG2208

COG function: function code TK; Serine phosphatase RsbU, regulator of sigma subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein. Note=Polar septum [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PP2C-like domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001932
- InterPro:   IPR014221
- InterPro:   IPR010822 [H]

Pfam domain/function: PF07228 SpoIIE [H]

EC number: =3.1.3.16 [H]

Molecular weight: Translated: 92176; Mature: 92176

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKAERRVNSPIAGPAVQKLYSWFGSMTKLMMQHLYSLFFYKGLIYMVIGFLLGRAFILS
CCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVIPFALPFFGAMLLIKKDKAFLACLALLAGALSISPQHSLFVLAALFAFAICSKMTSLI
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
IKDRVRTLPVVVFLAMAVTRCGFVYAEYGTVSGYHYIMAFVEAGLSFILTLIFLQSLPIV
HHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCH
TSKRAKQSLKIEEIICFMILIASVLTGLTGVSFQGMQAELILARYVVLAFAFIGGASIGC
HHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHH
TVGVVTGLILSLSNIGNLYQMSLLAFSGLLGGLLKEGKKFGAAVGLLIGSLLISLYGEGS
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
AELVPTLYESLIAIGLFLLTPQSITKKVAKYIPGTTEHAQEQQQYARKIRDVTAQKVDQF
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SNVFHALSESFATFYHSAPDDEGKEKEIDLFLSTVTEHSCQSCYKKNKCWVQNFDKTYDL
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCHHHHHH
MKRVMQETEEKQYFKNRKLKKEFHQHCSKSKQVEALIEDELTHFRANQTLKQKVHDSRRL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAEQLLGVSQVMADFSREIKREREQHFIQEEQIRDALQHFGIEIQQVEIYSLEQGNIDIE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCEEEE
MSIPYCNGHGECEKIIAPMLSDILEEQIIVKAEQCAGHPNGYCHVAFGSAKSYRVVTGAA
EECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEECCH
HAAKGGGLVSGDSYNMMELGTGKYAAAISDGMGNGARAHFESNETIKLLEKILQSGIDEK
HHCCCCCEECCCCCCEEEECCCCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCH
VAIKTINSILSLRTTDEIYSTLDLSVIDLQDASCKFLKIGSTPSFIKRGDQIIKVQASNL
HHHHHHHHHHHHCCHHHHHHHHCCEEEECCCCCCCEEEECCCHHHHHCCCEEEEEEECCC
PIGIITEFDVDVVSEQLKAGDLLIMMSDGIFEGPRHVENHDLWMKRKLKSLKTEEPQEIA
CEEEEECCCHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHH
DLIMEEVIRTRSGLIEDDMTVIVIKLDHNTPKWASIPAPAFFQKNQEIS
HHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHCCCCCCC
>Mature Secondary Structure
MEKAERRVNSPIAGPAVQKLYSWFGSMTKLMMQHLYSLFFYKGLIYMVIGFLLGRAFILS
CCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVIPFALPFFGAMLLIKKDKAFLACLALLAGALSISPQHSLFVLAALFAFAICSKMTSLI
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
IKDRVRTLPVVVFLAMAVTRCGFVYAEYGTVSGYHYIMAFVEAGLSFILTLIFLQSLPIV
HHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCH
TSKRAKQSLKIEEIICFMILIASVLTGLTGVSFQGMQAELILARYVVLAFAFIGGASIGC
HHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHH
TVGVVTGLILSLSNIGNLYQMSLLAFSGLLGGLLKEGKKFGAAVGLLIGSLLISLYGEGS
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
AELVPTLYESLIAIGLFLLTPQSITKKVAKYIPGTTEHAQEQQQYARKIRDVTAQKVDQF
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SNVFHALSESFATFYHSAPDDEGKEKEIDLFLSTVTEHSCQSCYKKNKCWVQNFDKTYDL
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCHHHHHH
MKRVMQETEEKQYFKNRKLKKEFHQHCSKSKQVEALIEDELTHFRANQTLKQKVHDSRRL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAEQLLGVSQVMADFSREIKREREQHFIQEEQIRDALQHFGIEIQQVEIYSLEQGNIDIE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCEEEE
MSIPYCNGHGECEKIIAPMLSDILEEQIIVKAEQCAGHPNGYCHVAFGSAKSYRVVTGAA
EECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEECCH
HAAKGGGLVSGDSYNMMELGTGKYAAAISDGMGNGARAHFESNETIKLLEKILQSGIDEK
HHCCCCCEECCCCCCEEEECCCCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCH
VAIKTINSILSLRTTDEIYSTLDLSVIDLQDASCKFLKIGSTPSFIKRGDQIIKVQASNL
HHHHHHHHHHHHCCHHHHHHHHCCEEEECCCCCCCEEEECCCHHHHHCCCEEEEEEECCC
PIGIITEFDVDVVSEQLKAGDLLIMMSDGIFEGPRHVENHDLWMKRKLKSLKTEEPQEIA
CEEEEECCCHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHH
DLIMEEVIRTRSGLIEDDMTVIVIKLDHNTPKWASIPAPAFFQKNQEIS
HHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7584024; 8830262; 9384377; 2832371; 8113187; 7570023 [H]