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The map label for this gene is tmk

Identifier: 52783885

GI number: 52783885

Start: 43427

End: 44065

Strand: Direct

Name: tmk

Synonym: BLi00041

Alternate gene names: 52783885

Gene position: 43427-44065 (Clockwise)

Preceding gene: 52783884

Following gene: 52783886

Centisome position: 1.03

GC content: 45.07

Gene sequence:

>639_bases
ATGAACGGTTTATTTATTACTTTTGAAGGGCCTGAAGGTGCGGGGAAAACGACAATCCTTCAGGCTGCGGCAGACCAATT
GACGAAAAACGGCCATTCTGTTCTTGCGACAAGGGAGCCGGGCGGCATTGAAATATCAGAACGAATTCGCGAGGTTATTT
TAAATCCAAGCCATACAGCAATGGATCCCAAAACAGAGGCCTTGTTGTATGCGGCCGCAAGAAGGCAGCACCTAGTCGAA
AAAGTAAAACCGGCGCTTGAAGAAGGGAAAATCGTCTTATGCGACCGGTTCATCGACAGCTCGCTTGCTTATCAGGGGTA
TGCCAGGGGACTCGGCATTGATGAAGTATTTTCTGTTAATCAATTCGCGATCGGCAGCCTGATGCCGAATGTGACGATTT
ATTTCGACATTGACCCGGAAGAGGGAATCAAACGAATCGATTTAAATGATGCAAGAGAGAAAAACCGGCTTGATTTGGAA
AAACTCCACTTTCATCAGCTTGTTCAAAAAGGCTACGAAGAAGTTATGAACCGGTTCCCCGGACGATTTCGGATTGTGGA
TGCCAGCCAAAGTGTTGAACTTGTGTTAAAAAGGGTAAATAACATCATTGAGGAAGCATTAAAAGAAAATCAGTTGTGA

Upstream 100 bases:

>100_bases
TCAGCCGGCTGACGCGGCTCGTCGATCTGAATGTACACATTCAGGGCAGCAATCAGCTCAAACAAAAGCAATTAACTGTA
TATATAGAAGAGGAGAAATC

Downstream 100 bases:

>100_bases
CCAGTTTACCATTCATTGTTATAATGGTAACAAGAGTATCCTTAAAGTGTTTCCATCCAAAAGGGGGCTTTTCATATGAA
GTTAATTATCGCTGTTGTTC

Product: thymidylate kinase

Products: NA

Alternate protein names: dTMP kinase

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MNGLFITFEGPEGAGKTTILQAAADQLTKNGHSVLATREPGGIEISERIREVILNPSHTAMDPKTEALLYAAARRQHLVE
KVKPALEEGKIVLCDRFIDSSLAYQGYARGLGIDEVFSVNQFAIGSLMPNVTIYFDIDPEEGIKRIDLNDAREKNRLDLE
KLHFHQLVQKGYEEVMNRFPGRFRIVDASQSVELVLKRVNNIIEEALKENQL

Sequences:

>Translated_212_residues
MNGLFITFEGPEGAGKTTILQAAADQLTKNGHSVLATREPGGIEISERIREVILNPSHTAMDPKTEALLYAAARRQHLVE
KVKPALEEGKIVLCDRFIDSSLAYQGYARGLGIDEVFSVNQFAIGSLMPNVTIYFDIDPEEGIKRIDLNDAREKNRLDLE
KLHFHQLVQKGYEEVMNRFPGRFRIVDASQSVELVLKRVNNIIEEALKENQL
>Mature_212_residues
MNGLFITFEGPEGAGKTTILQAAADQLTKNGHSVLATREPGGIEISERIREVILNPSHTAMDPKTEALLYAAARRQHLVE
KVKPALEEGKIVLCDRFIDSSLAYQGYARGLGIDEVFSVNQFAIGSLMPNVTIYFDIDPEEGIKRIDLNDAREKNRLDLE
KLHFHQLVQKGYEEVMNRFPGRFRIVDASQSVELVLKRVNNIIEEALKENQL

Specific function: Phosphorylation of dTMP to form dTDP in both de novo and salvage pathways of dTTP synthesis

COG id: COG0125

COG function: function code F; Thymidylate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate kinase family

Homologues:

Organism=Escherichia coli, GI1787340, Length=214, Percent_Identity=32.2429906542056, Blast_Score=107, Evalue=8e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KTHY_BACLD (Q65PJ3)

Other databases:

- EMBL:   CP000002
- EMBL:   AE017333
- RefSeq:   YP_077314.1
- RefSeq:   YP_089714.1
- HSSP:   P65248
- ProteinModelPortal:   Q65PJ3
- STRING:   Q65PJ3
- EnsemblBacteria:   EBBACT00000055779
- EnsemblBacteria:   EBBACT00000059043
- GeneID:   3028185
- GeneID:   3101520
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi00041
- KEGG:   bli:BL02509
- NMPDR:   fig|279010.5.peg.1324
- eggNOG:   COG0125
- GeneTree:   EBGT00050000002345
- HOGENOM:   HBG626009
- OMA:   KGHIVIS
- ProtClustDB:   PRK00698
- BioCyc:   BLIC279010-1:BLI00041-MONOMER
- BioCyc:   BLIC279010:BL02509-MONOMER
- HAMAP:   MF_00165
- InterPro:   IPR018095
- InterPro:   IPR018094
- PANTHER:   PTHR10344
- TIGRFAMs:   TIGR00041

Pfam domain/function: PF02223 Thymidylate_kin

EC number: =2.7.4.9

Molecular weight: Translated: 23818; Mature: 23818

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS01331 THYMIDYLATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGLFITFEGPEGAGKTTILQAAADQLTKNGHSVLATREPGGIEISERIREVILNPSHTA
CCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHCCCCCCC
MDPKTEALLYAAARRQHLVEKVKPALEEGKIVLCDRFIDSSLAYQGYARGLGIDEVFSVN
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHCCCCHHHHHHCC
QFAIGSLMPNVTIYFDIDPEEGIKRIDLNDAREKNRLDLEKLHFHQLVQKGYEEVMNRFP
HHHHHHHCCCEEEEEEECHHHCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
GRFRIVDASQSVELVLKRVNNIIEEALKENQL
CEEEEEECCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNGLFITFEGPEGAGKTTILQAAADQLTKNGHSVLATREPGGIEISERIREVILNPSHTA
CCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHCCCCCCC
MDPKTEALLYAAARRQHLVEKVKPALEEGKIVLCDRFIDSSLAYQGYARGLGIDEVFSVN
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHCCCCHHHHHHCC
QFAIGSLMPNVTIYFDIDPEEGIKRIDLNDAREKNRLDLEKLHFHQLVQKGYEEVMNRFP
HHHHHHHCCCEEEEEEECHHHCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
GRFRIVDASQSVELVLKRVNNIIEEALKENQL
CEEEEEECCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA