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The map label for this gene is yaaO [H]

Identifier: 52783884

GI number: 52783884

Start: 41910

End: 43430

Strand: Direct

Name: yaaO [H]

Synonym: BLi00040

Alternate gene names: 52783884

Gene position: 41910-43430 (Clockwise)

Preceding gene: 52783883

Following gene: 52783885

Centisome position: 0.99

GC content: 46.88

Gene sequence:

>1521_bases
ATGCAGAATGTCATATCGGCATCCGGCAGCGCCTGGATGCCGAAGAAAATAGAGAAGATGACTAAAGGATCGGTTTATAT
GAAGACACCTTTATATACAGCATTGGTGAACCATGCTGAAGGACATCATTATTCTTTTCACGTTCCGGGACATCACAATG
GAGATGTCTTTTTTGATGAAGCAAAAACTTTTTTTGAAACGATTCTCAAAGTGGATCTAACTGAACTGACAGGACTGGAT
GATTTACATGAGCCATCTGGCGTCATCAAGGAAGCACAGGATTTAGTTTCGCGGCTTTACGGAGCGGAAGAAAGCTTTTT
TCTCGTCAATGGTTCGACCGTTGGGAATCTGGCTATGATCCTGGCTGTTTGTCAGCCGGGTGATACGATACTCGTTCAGC
GTAACTGCCATAAGTCTGTATTTCATGCGATTGAACTTTCAGGCGCCCATCCGGTTTTTTTGACACCGGAAATTGATGAG
GCGATGGCTGTTCCCACACATATACTGTACGAAACAGTTGAAGATGCAATTTCACAATATCCGCACGCAAAAGGGATCGT
GTTGACATATCCAAATTACTACGGCCATGCAGTTGATCTGAAGCCGATCATAGAAAAGGCCCACCAACACGATATTTCCG
TTTTAGTCGACGAGGCTCACGGCGCCCATTTTGTGCTGGGCCATCCATTTCCCCAGTCGTCGTTGAAAGCGGGCGCTGAT
GCCGTCGTCCAGTCCGCGCATAAAACGCTCCCGGCGATGACGATGGGGTCCTATCTGCATCTTAACAGCGGCAGGATCAA
CAGGGACAGACTGGCATACTATCTGTCCGTGCTTCAAAGCAGCAGTCCTTCCTACCCAATTATGGCATCTTTGGATATAG
CTAGGGCATACGCCGAAGATATCTTGAAAACAAATCGAACAGCCGATATTGAGAAAGAGCTCATCAATATGAGAGAAGTC
TTTTCTCAAATAAACGGAGCTGATATTGTCGAGCCGGCTGATGCCCGTATCCGGCAAGATCCGCTTAAGCTGTGTATCAG
ATCAGCGTACGGCCACTCCGGTTTTGAACTGAAATCCATATTTGAAGCAAACGGCATCCATCCTGAATTGGCAGATGAAA
GGCAAGTCCTGTTGATCCTGCCGCTTGAAGGAAAAAATATGCCCGCGCCGGAACTGATCAGCACAATCAGTAAAGACATG
AAAGATACAGCAGTTCGAAATGATCTTCCTGCCGGAATCGGAATCCCTTCTGAAAAAGTCACGGCTTTGCCTTACCGGAA
AAGCAAGTTGTCTGCCTTCAAAAAAGAATCGGTGCCTTTTACCGAAGCAGCGGGCAGAATAAGCGCCGAATCGGTCACAC
CTTATCCTCCGGGCATTCCGTTGATCATGGCGGGTGAGAGAATAACAAAAGAGACGATCAGCCGGCTGACGCGGCTCGTC
GATCTGAATGTACACATTCAGGGCAGCAATCAGCTCAAACAAAAGCAATTAACTGTATATATAGAAGAGGAGAAATCATG
A

Upstream 100 bases:

>100_bases
TGCTGGACATGAAAGAAAACCAAAACCGTAGATCATAAGCAAGATTAACATTTACCAAATGGTCCGCCCTTTATGTTATC
CGCCTTGAAGGGTATAATAA

Downstream 100 bases:

>100_bases
ACGGTTTATTTATTACTTTTGAAGGGCCTGAAGGTGCGGGGAAAACGACAATCCTTCAGGCTGCGGCAGACCAATTGACG
AAAAACGGCCATTCTGTTCT

Product: YaaO

Products: Agmatine; CO2 [C]

Alternate protein names: NA

Number of amino acids: Translated: 506; Mature: 506

Protein sequence:

>506_residues
MQNVISASGSAWMPKKIEKMTKGSVYMKTPLYTALVNHAEGHHYSFHVPGHHNGDVFFDEAKTFFETILKVDLTELTGLD
DLHEPSGVIKEAQDLVSRLYGAEESFFLVNGSTVGNLAMILAVCQPGDTILVQRNCHKSVFHAIELSGAHPVFLTPEIDE
AMAVPTHILYETVEDAISQYPHAKGIVLTYPNYYGHAVDLKPIIEKAHQHDISVLVDEAHGAHFVLGHPFPQSSLKAGAD
AVVQSAHKTLPAMTMGSYLHLNSGRINRDRLAYYLSVLQSSSPSYPIMASLDIARAYAEDILKTNRTADIEKELINMREV
FSQINGADIVEPADARIRQDPLKLCIRSAYGHSGFELKSIFEANGIHPELADERQVLLILPLEGKNMPAPELISTISKDM
KDTAVRNDLPAGIGIPSEKVTALPYRKSKLSAFKKESVPFTEAAGRISAESVTPYPPGIPLIMAGERITKETISRLTRLV
DLNVHIQGSNQLKQKQLTVYIEEEKS

Sequences:

>Translated_506_residues
MQNVISASGSAWMPKKIEKMTKGSVYMKTPLYTALVNHAEGHHYSFHVPGHHNGDVFFDEAKTFFETILKVDLTELTGLD
DLHEPSGVIKEAQDLVSRLYGAEESFFLVNGSTVGNLAMILAVCQPGDTILVQRNCHKSVFHAIELSGAHPVFLTPEIDE
AMAVPTHILYETVEDAISQYPHAKGIVLTYPNYYGHAVDLKPIIEKAHQHDISVLVDEAHGAHFVLGHPFPQSSLKAGAD
AVVQSAHKTLPAMTMGSYLHLNSGRINRDRLAYYLSVLQSSSPSYPIMASLDIARAYAEDILKTNRTADIEKELINMREV
FSQINGADIVEPADARIRQDPLKLCIRSAYGHSGFELKSIFEANGIHPELADERQVLLILPLEGKNMPAPELISTISKDM
KDTAVRNDLPAGIGIPSEKVTALPYRKSKLSAFKKESVPFTEAAGRISAESVTPYPPGIPLIMAGERITKETISRLTRLV
DLNVHIQGSNQLKQKQLTVYIEEEKS
>Mature_506_residues
MQNVISASGSAWMPKKIEKMTKGSVYMKTPLYTALVNHAEGHHYSFHVPGHHNGDVFFDEAKTFFETILKVDLTELTGLD
DLHEPSGVIKEAQDLVSRLYGAEESFFLVNGSTVGNLAMILAVCQPGDTILVQRNCHKSVFHAIELSGAHPVFLTPEIDE
AMAVPTHILYETVEDAISQYPHAKGIVLTYPNYYGHAVDLKPIIEKAHQHDISVLVDEAHGAHFVLGHPFPQSSLKAGAD
AVVQSAHKTLPAMTMGSYLHLNSGRINRDRLAYYLSVLQSSSPSYPIMASLDIARAYAEDILKTNRTADIEKELINMREV
FSQINGADIVEPADARIRQDPLKLCIRSAYGHSGFELKSIFEANGIHPELADERQVLLILPLEGKNMPAPELISTISKDM
KDTAVRNDLPAGIGIPSEKVTALPYRKSKLSAFKKESVPFTEAAGRISAESVTPYPPGIPLIMAGERITKETISRLTRLV
DLNVHIQGSNQLKQKQLTVYIEEEKS

Specific function: Adc Can Be Found In Two Forms: Biodegradative And Biosynthetic. The Biodegradative Form May Play A Role In Regulating pH By Consuming Proteins. [C]

COG id: COG1982

COG function: function code E; Arginine/lysine/ornithine decarboxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Orn/Lys/Arg decarboxylase class-I family [H]

Homologues:

Organism=Escherichia coli, GI221142684, Length=365, Percent_Identity=25.4794520547945, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1786384, Length=294, Percent_Identity=27.2108843537415, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1786909, Length=579, Percent_Identity=21.0708117443869, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1790573, Length=285, Percent_Identity=25.2631578947368, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI87082193, Length=300, Percent_Identity=24.6666666666667, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000310
- InterPro:   IPR008286
- InterPro:   IPR015424
- InterPro:   IPR015421 [H]

Pfam domain/function: PF01276 OKR_DC_1; PF03711 OKR_DC_1_C [H]

EC number: 4.1.1.19 [C]

Molecular weight: Translated: 55788; Mature: 55788

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNVISASGSAWMPKKIEKMTKGSVYMKTPLYTALVNHAEGHHYSFHVPGHHNGDVFFDE
CCCCCCCCCCCCCHHHHHHHHCCCEEEECCHHHHHHHCCCCCEEEEECCCCCCCCEEEHH
AKTFFETILKVDLTELTGLDDLHEPSGVIKEAQDLVSRLYGAEESFFLVNGSTVGNLAMI
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHH
LAVCQPGDTILVQRNCHKSVFHAIELSGAHPVFLTPEIDEAMAVPTHILYETVEDAISQY
HEEECCCCEEEEECCHHHHHHHHHEECCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHC
PHAKGIVLTYPNYYGHAVDLKPIIEKAHQHDISVLVDEAHGAHFVLGHPFPQSSLKAGAD
CCCCCEEEECCCCCCCEECHHHHHHHHHCCCEEEEEECCCCCEEEEECCCCHHHHHCCHH
AVVQSAHKTLPAMTMGSYLHLNSGRINRDRLAYYLSVLQSSSPSYPIMASLDIARAYAED
HHHHHHHHHCCHHHCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHH
ILKTNRTADIEKELINMREVFSQINGADIVEPADARIRQDPLKLCIRSAYGHSGFELKSI
HHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHH
FEANGIHPELADERQVLLILPLEGKNMPAPELISTISKDMKDTAVRNDLPAGIGIPSEKV
HHCCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCE
TALPYRKSKLSAFKKESVPFTEAAGRISAESVTPYPPGIPLIMAGERITKETISRLTRLV
EECCCCHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHE
DLNVHIQGSNQLKQKQLTVYIEEEKS
EEEEEEECCCCCCCCEEEEEEECCCC
>Mature Secondary Structure
MQNVISASGSAWMPKKIEKMTKGSVYMKTPLYTALVNHAEGHHYSFHVPGHHNGDVFFDE
CCCCCCCCCCCCCHHHHHHHHCCCEEEECCHHHHHHHCCCCCEEEEECCCCCCCCEEEHH
AKTFFETILKVDLTELTGLDDLHEPSGVIKEAQDLVSRLYGAEESFFLVNGSTVGNLAMI
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHH
LAVCQPGDTILVQRNCHKSVFHAIELSGAHPVFLTPEIDEAMAVPTHILYETVEDAISQY
HEEECCCCEEEEECCHHHHHHHHHEECCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHC
PHAKGIVLTYPNYYGHAVDLKPIIEKAHQHDISVLVDEAHGAHFVLGHPFPQSSLKAGAD
CCCCCEEEECCCCCCCEECHHHHHHHHHCCCEEEEEECCCCCEEEEECCCCHHHHHCCHH
AVVQSAHKTLPAMTMGSYLHLNSGRINRDRLAYYLSVLQSSSPSYPIMASLDIARAYAED
HHHHHHHHHCCHHHCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHH
ILKTNRTADIEKELINMREVFSQINGADIVEPADARIRQDPLKLCIRSAYGHSGFELKSI
HHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHH
FEANGIHPELADERQVLLILPLEGKNMPAPELISTISKDMKDTAVRNDLPAGIGIPSEKV
HHCCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCE
TALPYRKSKLSAFKKESVPFTEAAGRISAESVTPYPPGIPLIMAGERITKETISRLTRLV
EECCCCHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHE
DLNVHIQGSNQLKQKQLTVYIEEEKS
EEEEEEECCCCCCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: 2-Norpyridoxal phosphate; 6-Methylpyridoxal phosphate; Pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 1.2 {L-canavanine}} 0.65 {L-Arg}} [C]

Substrates: L-Arginine; H+ [C]

Specific reaction: L-Arginine + H+ --> Agmatine + CO2 [C]

General reaction: Decarboxylation [C]

Inhibitor: 2-Nor-2-butyl pyridoxalphosphate; 5-Deoxypyridoxal; Ag+; Agmatine; Citrulline; Guanidine; Guanidinoacetic acid; L-2-Amino-3-guanidinopropanoate; L-2-Amino-4-guanidinobutyrate; L-2-Amino-5-guanidinopentanoate; L-2-Amino-6-guanidinohexanoate; L-2-Chloro-5-g

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]