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The map label for this gene is iolD

Identifier: 52142985

GI number: 52142985

Start: 2385021

End: 2386955

Strand: Direct

Name: iolD

Synonym: BCZK2254

Alternate gene names: 52142985

Gene position: 2385021-2386955 (Clockwise)

Preceding gene: 52142987

Following gene: 52142983

Centisome position: 44.99

GC content: 38.81

Gene sequence:

>1935_bases
ATGCAAACTGTTAGAATGACCACGGCGCAAGCATTGGTGAAATTTTTAAATCAGCAATACGTAGAGTTTGATGGAAAGCA
ACAAAAGTTTATTAAGGGGATCTTTACAATTTTTGGTCATGGAAATGTAGTAGGTCTAGGTCAAGCTTTAGAAGAAGATG
CAGGGGAATTAGAAGTATATCAAGGGAGAAATGAGCAAGGAATGGCAAATGCTGCAATGGCTTTTGCAAAACAAAAACAT
AGAAAACAAATTATGGCATGTACTTCTTCAGTTGGTCCTGGATCAGCAAATATGATTACCTCTGCAGCAACAGCTTCTGC
AAATAATATTCCCGTTTTATTACTTCCAGGAGATGTATTTGCAACGAGACAGCCTGATCCTGTTCTTCAACAAATTGAAC
AAACACATGATTTATCTATTTCTACAAATGATGCTTTCCGTGCAGTAAGTAAGTACTGGGACAGGATAAATCGTCCTGAA
CAATTGATGACAGCTATGATTCAAGCCATGCGTGTTTTAACGAATCCGGCAGATACAGGAGCTGTAACAATCTGTTTACC
ACAAGATGTCCAAGGAGAAGCATGGGATTTTCCAAGTTACTTCTTTCAAAAGCGTGTTCACCGTATTGAACGCCGTCTAC
CTACAAAAGCCAGCTTAGCGGATGCAGTGGAAATGATTAAGAGAAAGAAAAAGCCAGTTATGATTTGCGGTGGTGGTGTA
AGATACGCAGAAGCGGCAGAAGAACTTAAACAGTTTGCTGAAACATTCCACATTCCATTTGGAGAAACACAGGCTGGAAA
AAGTGCAATTGAAAGTAGTCATCCATATAATCTTGGTGGTATTGGGGTAACGGGAAATATAGCAGCTAATACAATTGCAA
AGGAAGCGGATCTTGTTATTGGCATCGGGACGAGATTTACTGATTTTACAACGGCATCGAAACAATTATTTCAAAATGAG
GAAGTTGAGTTTGTAAACATCAATATTTCAGAATTCCATGCGAATAAGCTGGATGCATTAAAGGTTATAGCAGATGCGAA
AGAAGCGCTTCTTACTCTAATAGATGAACTGCAAGTAATTGATTATCGATCTAGTTATACAGTAGAAATTGCTGATGCAA
AAGAGGCTTGGGAAACAGAATTATCACGTCTACATAATATTCGCTTTACAGGTCAAGATTTCACACCAGAAGTAGAAGGC
CATTTTGATGGAAATTTAAATGAGTATGTAGACGCACTTGGTTCACAATTAACACAGACTGCAGTCATTGGACAAATCAA
TACATTACTTGATGAAGATGCAATTATCGTTGGAGCGGCAGGAAGTCTTCCAGGTGATTTACAAAGAATGTGGGCATCAA
GAAAACCAAATACATATCACATGGAGTATGGATACTCTTGCATGGGCTATGAGGTTGCGGGCGCACTAGGTGCAAAGATA
GCTGAACCATCGAAGGAAGTATATGCAATGGTAGGGGATGGTAGTTACCAGATGCTTCATTCTGAGCTTGTCACAAGTCT
CCAAGAAAACAAAAAAATTAACGTCTTATTGTTTGATAACTCCGGATTTGGTTGCATTAATAACTTACAAATGGGCAATG
GAATGGGGAGCTTTGGAACAGAGTTTCGCTACCGAAACCAGGAAACTCGTAAATTAGACGGGGCAATTATGAAAATTGAT
TTTGCAGCTAGCGCAGCTGGATATGGTGTGAAAACATATCATGTTACATCACTAGAACAATTAAGGGAAGCGCTTATAGA
TGCGAAAAAACAAACAGTTTCCACATTAATTGATATTAAAGTATTACCAAAAACAATGACAAATGGATACGAGTCATGGT
GGCATGTAGGTGTTGCAGAAGTATCTAAAAGTCAAAGTGTACAAGCTGCGTACGAGAGTAAAGTAAGTAACTTGCAACAG
GCAAGGTCTTATTAG

Upstream 100 bases:

>100_bases
GTAGTAAAACATAAGAAGGAAGTATTAGGAATCTATCAAATTCCTGATACTCCTTTTTCATATGCAGAATGACTGATAGA
GATAGAAAGGAGCAAATCAT

Downstream 100 bases:

>100_bases
ATTAGGACACAAAGAGAGCTTTTTATATACATGCATATTAATTAGGAAGCTCTCTTTTCAATAACCAGTTAAGAGAATTT
GGCGAAATTCTATCACATTT

Product: myo-inositol catabolism protein, malonic semialdehyde oxidative decarboxylase

Products: pyruvate; 2-aceto-2-hydroxy-butyrate; CO2 [C]

Alternate protein names: THcHDO hydrolase 1

Number of amino acids: Translated: 644; Mature: 644

Protein sequence:

>644_residues
MQTVRMTTAQALVKFLNQQYVEFDGKQQKFIKGIFTIFGHGNVVGLGQALEEDAGELEVYQGRNEQGMANAAMAFAKQKH
RKQIMACTSSVGPGSANMITSAATASANNIPVLLLPGDVFATRQPDPVLQQIEQTHDLSISTNDAFRAVSKYWDRINRPE
QLMTAMIQAMRVLTNPADTGAVTICLPQDVQGEAWDFPSYFFQKRVHRIERRLPTKASLADAVEMIKRKKKPVMICGGGV
RYAEAAEELKQFAETFHIPFGETQAGKSAIESSHPYNLGGIGVTGNIAANTIAKEADLVIGIGTRFTDFTTASKQLFQNE
EVEFVNINISEFHANKLDALKVIADAKEALLTLIDELQVIDYRSSYTVEIADAKEAWETELSRLHNIRFTGQDFTPEVEG
HFDGNLNEYVDALGSQLTQTAVIGQINTLLDEDAIIVGAAGSLPGDLQRMWASRKPNTYHMEYGYSCMGYEVAGALGAKI
AEPSKEVYAMVGDGSYQMLHSELVTSLQENKKINVLLFDNSGFGCINNLQMGNGMGSFGTEFRYRNQETRKLDGAIMKID
FAASAAGYGVKTYHVTSLEQLREALIDAKKQTVSTLIDIKVLPKTMTNGYESWWHVGVAEVSKSQSVQAAYESKVSNLQQ
ARSY

Sequences:

>Translated_644_residues
MQTVRMTTAQALVKFLNQQYVEFDGKQQKFIKGIFTIFGHGNVVGLGQALEEDAGELEVYQGRNEQGMANAAMAFAKQKH
RKQIMACTSSVGPGSANMITSAATASANNIPVLLLPGDVFATRQPDPVLQQIEQTHDLSISTNDAFRAVSKYWDRINRPE
QLMTAMIQAMRVLTNPADTGAVTICLPQDVQGEAWDFPSYFFQKRVHRIERRLPTKASLADAVEMIKRKKKPVMICGGGV
RYAEAAEELKQFAETFHIPFGETQAGKSAIESSHPYNLGGIGVTGNIAANTIAKEADLVIGIGTRFTDFTTASKQLFQNE
EVEFVNINISEFHANKLDALKVIADAKEALLTLIDELQVIDYRSSYTVEIADAKEAWETELSRLHNIRFTGQDFTPEVEG
HFDGNLNEYVDALGSQLTQTAVIGQINTLLDEDAIIVGAAGSLPGDLQRMWASRKPNTYHMEYGYSCMGYEVAGALGAKI
AEPSKEVYAMVGDGSYQMLHSELVTSLQENKKINVLLFDNSGFGCINNLQMGNGMGSFGTEFRYRNQETRKLDGAIMKID
FAASAAGYGVKTYHVTSLEQLREALIDAKKQTVSTLIDIKVLPKTMTNGYESWWHVGVAEVSKSQSVQAAYESKVSNLQQ
ARSY
>Mature_644_residues
MQTVRMTTAQALVKFLNQQYVEFDGKQQKFIKGIFTIFGHGNVVGLGQALEEDAGELEVYQGRNEQGMANAAMAFAKQKH
RKQIMACTSSVGPGSANMITSAATASANNIPVLLLPGDVFATRQPDPVLQQIEQTHDLSISTNDAFRAVSKYWDRINRPE
QLMTAMIQAMRVLTNPADTGAVTICLPQDVQGEAWDFPSYFFQKRVHRIERRLPTKASLADAVEMIKRKKKPVMICGGGV
RYAEAAEELKQFAETFHIPFGETQAGKSAIESSHPYNLGGIGVTGNIAANTIAKEADLVIGIGTRFTDFTTASKQLFQNE
EVEFVNINISEFHANKLDALKVIADAKEALLTLIDELQVIDYRSSYTVEIADAKEAWETELSRLHNIRFTGQDFTPEVEG
HFDGNLNEYVDALGSQLTQTAVIGQINTLLDEDAIIVGAAGSLPGDLQRMWASRKPNTYHMEYGYSCMGYEVAGALGAKI
AEPSKEVYAMVGDGSYQMLHSELVTSLQENKKINVLLFDNSGFGCINNLQMGNGMGSFGTEFRYRNQETRKLDGAIMKID
FAASAAGYGVKTYHVTSLEQLREALIDAKKQTVSTLIDIKVLPKTMTNGYESWWHVGVAEVSKSQSVQAAYESKVSNLQQ
ARSY

Specific function: Involved in the cleavage of the C1-C2 bond of 3D- (3,5/4)-trihydroxycyclohexane-1,2-dione (THcHDO) to yield 5-deoxy- glucuronate (5DG)

COG id: COG3962

COG function: function code E; Acetolactate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family

Homologues:

Organism=Homo sapiens, GI93004078, Length=566, Percent_Identity=20.6713780918728, Blast_Score=89, Evalue=1e-17,
Organism=Escherichia coli, GI1790104, Length=604, Percent_Identity=23.841059602649, Blast_Score=152, Evalue=7e-38,
Organism=Escherichia coli, GI1786717, Length=567, Percent_Identity=25.3968253968254, Blast_Score=135, Evalue=1e-32,
Organism=Escherichia coli, GI87081685, Length=584, Percent_Identity=24.8287671232877, Blast_Score=125, Evalue=9e-30,
Organism=Escherichia coli, GI1787096, Length=530, Percent_Identity=22.4528301886792, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI1788716, Length=532, Percent_Identity=21.2406015037594, Blast_Score=97, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17531301, Length=449, Percent_Identity=24.2761692650334, Blast_Score=82, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17531299, Length=449, Percent_Identity=24.2761692650334, Blast_Score=82, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6323755, Length=597, Percent_Identity=24.1206030150754, Blast_Score=132, Evalue=2e-31,
Organism=Drosophila melanogaster, GI19922626, Length=619, Percent_Identity=22.4555735056543, Blast_Score=100, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): IOLD1_BACCZ (Q63B73)

Other databases:

- EMBL:   CP000001
- RefSeq:   YP_083844.1
- HSSP:   P07342
- ProteinModelPortal:   Q63B73
- STRING:   Q63B73
- EnsemblBacteria:   EBBACT00000043404
- GeneID:   3025505
- GenomeReviews:   CP000001_GR
- KEGG:   bcz:BCZK2254
- eggNOG:   COG3962
- GeneTree:   EBGT00070000032034
- HOGENOM:   HBG294332
- OMA:   GAIMKID
- ProtClustDB:   CLSK2304880
- BioCyc:   BCER288681:BCE33L2254-MONOMER
- HAMAP:   MF_01669
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N

EC number: 2.2.1.6 [C]

Molecular weight: Translated: 70941; Mature: 70941

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS00187 TPP_ENZYMES

Important sites: BINDING 65-65

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTVRMTTAQALVKFLNQQYVEFDGKQQKFIKGIFTIFGHGNVVGLGQALEEDAGELEVY
CCCEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEECCHHHHCCCCCEEEE
QGRNEQGMANAAMAFAKQKHRKQIMACTSSVGPGSANMITSAATASANNIPVLLLPGDVF
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHEEHHHCCCCCCCEEEEECCCEE
ATRQPDPVLQQIEQTHDLSISTNDAFRAVSKYWDRINRPEQLMTAMIQAMRVLTNPADTG
CCCCCCHHHHHHHHHCCCEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCC
AVTICLPQDVQGEAWDFPSYFFQKRVHRIERRLPTKASLADAVEMIKRKKKPVMICGGGV
EEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEECCCC
RYAEAAEELKQFAETFHIPFGETQAGKSAIESSHPYNLGGIGVTGNIAANTIAKEADLVI
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCEEE
GIGTRFTDFTTASKQLFQNEEVEFVNINISEFHANKLDALKVIADAKEALLTLIDELQVI
ECCCCCCCHHHHHHHHHCCCCEEEEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHE
DYRSSYTVEIADAKEAWETELSRLHNIRFTGQDFTPEVEGHFDGNLNEYVDALGSQLTQT
EECCCEEEEECCCHHHHHHHHHHHHCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
AVIGQINTLLDEDAIIVGAAGSLPGDLQRMWASRKPNTYHMEYGYSCMGYEVAGALGAKI
HHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHCCCCCEEEEECCCEEECHHHHHHHHHHC
AEPSKEVYAMVGDGSYQMLHSELVTSLQENKKINVLLFDNSGFGCINNLQMGNGMGSFGT
CCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEECCCCCCCCCCCCCC
EFRYRNQETRKLDGAIMKIDFAASAAGYGVKTYHVTSLEQLREALIDAKKQTVSTLIDIK
CCEECCCHHHHHCCEEEEEEECCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHE
VLPKTMTNGYESWWHVGVAEVSKSQSVQAAYESKVSNLQQARSY
EECHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQTVRMTTAQALVKFLNQQYVEFDGKQQKFIKGIFTIFGHGNVVGLGQALEEDAGELEVY
CCCEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEECCHHHHCCCCCEEEE
QGRNEQGMANAAMAFAKQKHRKQIMACTSSVGPGSANMITSAATASANNIPVLLLPGDVF
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHEEHHHCCCCCCCEEEEECCCEE
ATRQPDPVLQQIEQTHDLSISTNDAFRAVSKYWDRINRPEQLMTAMIQAMRVLTNPADTG
CCCCCCHHHHHHHHHCCCEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCC
AVTICLPQDVQGEAWDFPSYFFQKRVHRIERRLPTKASLADAVEMIKRKKKPVMICGGGV
EEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEECCCC
RYAEAAEELKQFAETFHIPFGETQAGKSAIESSHPYNLGGIGVTGNIAANTIAKEADLVI
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCEEE
GIGTRFTDFTTASKQLFQNEEVEFVNINISEFHANKLDALKVIADAKEALLTLIDELQVI
ECCCCCCCHHHHHHHHHCCCCEEEEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHE
DYRSSYTVEIADAKEAWETELSRLHNIRFTGQDFTPEVEGHFDGNLNEYVDALGSQLTQT
EECCCEEEEECCCHHHHHHHHHHHHCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
AVIGQINTLLDEDAIIVGAAGSLPGDLQRMWASRKPNTYHMEYGYSCMGYEVAGALGAKI
HHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHCCCCCEEEEECCCEEECHHHHHHHHHHC
AEPSKEVYAMVGDGSYQMLHSELVTSLQENKKINVLLFDNSGFGCINNLQMGNGMGSFGT
CCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEECCCCCCCCCCCCCC
EFRYRNQETRKLDGAIMKIDFAASAAGYGVKTYHVTSLEQLREALIDAKKQTVSTLIDIK
CCEECCCHHHHHCCEEEEEEECCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHE
VLPKTMTNGYESWWHVGVAEVSKSQSVQAAYESKVSNLQQARSY
EECHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FAD [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 4002 [C]

Specific activity: 53

Km value (mM): 7.6 {pyruvate}} 7 {pyruvate}} 2.9 {pyruvate}} 2.6 {pyruvate}} 1.3 {pyruvate}} 1 {pyruvate}} 5 {2-oxobutanoate}} 1 {2-oxobutanoate}} [C]

Substrates: acetolactate; CO2; pyruvate; 2-oxobutanoate [C]

Specific reaction: acetolactate + CO2 = 2 pyruvate pyruvate + 2-oxobutanoate = 2-aceto-2-hydroxy-butyrate + CO2 [C]

General reaction: C-C bond formation; decarboxylation [C]

Inhibitor: @OXOB02.txt*2-oxobutanoate!; chlorimuronethyl; chlorsulphuron; Ile; imazapyr; Leu; Val [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA