BacMap

Definition	Bacillus cereus E33L, complete genome.
Accession	NC_006274
Length	5,300,915

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The map label for this gene is crt [H]

Identifier: 52142950

GI number: 52142950

Start: 2426682

End: 2427470

Strand: Direct

Name: crt [H]

Synonym: BCZK2290

Alternate gene names: 52142950

Gene position: 2426682-2427470 (Clockwise)

Preceding gene: 52142952

Following gene: 52142949

Centisome position: 45.78

GC content: 37.01

Gene sequence:

>789_bases
ATGCTACAATTACAAAATATTTCAGTTGATTATGTAACACCGCACGTTGTAAAGATTTCATTAAATCGTGAAAGACAAGC
AAACTCATTATCTTTAGCGTTATTAGAAGAGTTACAAAACATATTAACTCAAATAAATGAAGAGGCAAATACTCGTGTAG
TTATTTTAACAGGTGCTGGTGAAAAAGCGTTTTGTGCTGGTGCTGATTTAAAAGAACGTGCTGGCATGAATGAAGAACAA
GTTCGCCATGCTGTTAGTATGATTCGTACTACTATGGAAATGGTTGAACAATTACCACAGCCAGTTATTGCTGCTGTAAA
TGGAATCGCACTTGGTGGTGGTACGGAATTATGTTTAGCTTGTGATTTTAGAATTGCGGCTGAATCTGCAAGTCTTGGTC
TTACTGAAACTACACTTGCAATTATACCAGGAGCAGGTGGTACGCAGCGTTTGCCAAGATTAATTGGTGTTGGTAGAGCG
AAAGAATTAATTTATACAGGAAGACGTATTTCGGCACAAGAAGCGAAAGAATATGGTCTAGTAGAATTTGTTGTACCAGC
TCATTTACTTGAAGAAAAAGCGATTGAAATAGCAGAGAAAATTGCTAGTAATGGCCCTATTGCTGTTCGATTAGCGAAAG
AAGCAATTTCAAACGGTATTCAAGTTGATTTACATACCGGATTACAAATGGAAAAACAAGCGTATGAAGGTGTAATCCAT
ACGAAAGATAGAATAGAAGGATTACAAGCATTCAAGGAAAAACGCACACCAATGTATAAGGGGGAGTAA

Upstream 100 bases:

>100_bases
TTATTGAGAGCGAGTCAATTTATTCAAAGTAAATTAAATATCCAATTACCGAGTCATGTGTATAGAGCGCTTCAACATAA
AACGATAAGTAGGTGAGAAG

Downstream 100 bases:

>100_bases
ATATGATAGATCAAAAAGAACAATCGAATACATTTGAAGAACGAGTTGAAACGATTAAACAAGGCGGCGCACCAAAATAT
CATGATCAAAACAAAGCGAA

Product: enoyl-CoA hydratase

Products: crotonoyl-CoA; H2O

Alternate protein names: NA

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MLQLQNISVDYVTPHVVKISLNRERQANSLSLALLEELQNILTQINEEANTRVVILTGAGEKAFCAGADLKERAGMNEEQ
VRHAVSMIRTTMEMVEQLPQPVIAAVNGIALGGGTELCLACDFRIAAESASLGLTETTLAIIPGAGGTQRLPRLIGVGRA
KELIYTGRRISAQEAKEYGLVEFVVPAHLLEEKAIEIAEKIASNGPIAVRLAKEAISNGIQVDLHTGLQMEKQAYEGVIH
TKDRIEGLQAFKEKRTPMYKGE

Sequences:

>Translated_262_residues
MLQLQNISVDYVTPHVVKISLNRERQANSLSLALLEELQNILTQINEEANTRVVILTGAGEKAFCAGADLKERAGMNEEQ
VRHAVSMIRTTMEMVEQLPQPVIAAVNGIALGGGTELCLACDFRIAAESASLGLTETTLAIIPGAGGTQRLPRLIGVGRA
KELIYTGRRISAQEAKEYGLVEFVVPAHLLEEKAIEIAEKIASNGPIAVRLAKEAISNGIQVDLHTGLQMEKQAYEGVIH
TKDRIEGLQAFKEKRTPMYKGE
>Mature_262_residues
MLQLQNISVDYVTPHVVKISLNRERQANSLSLALLEELQNILTQINEEANTRVVILTGAGEKAFCAGADLKERAGMNEEQ
VRHAVSMIRTTMEMVEQLPQPVIAAVNGIALGGGTELCLACDFRIAAESASLGLTETTLAIIPGAGGTQRLPRLIGVGRA
KELIYTGRRISAQEAKEYGLVEFVVPAHLLEEKAIEIAEKIASNGPIAVRLAKEAISNGIQVDLHTGLQMEKQAYEGVIH
TKDRIEGLQAFKEKRTPMYKGE

Specific function: Could Possibly Oxidizes Fatty Acids Using Specific Components (By Similarity). [C]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI4502327, Length=256, Percent_Identity=48.4375, Blast_Score=207, Evalue=8e-54,
Organism=Homo sapiens, GI194097323, Length=244, Percent_Identity=42.6229508196721, Blast_Score=187, Evalue=1e-47,
Organism=Homo sapiens, GI150378541, Length=251, Percent_Identity=38.6454183266932, Blast_Score=161, Evalue=7e-40,
Organism=Homo sapiens, GI31542718, Length=251, Percent_Identity=34.2629482071713, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI70995211, Length=254, Percent_Identity=30.3149606299213, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI68989263, Length=201, Percent_Identity=35.8208955223881, Blast_Score=111, Evalue=7e-25,
Organism=Homo sapiens, GI20127408, Length=210, Percent_Identity=35.2380952380952, Blast_Score=107, Evalue=9e-24,
Organism=Homo sapiens, GI261878539, Length=115, Percent_Identity=44.3478260869565, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI37594471, Length=196, Percent_Identity=30.1020408163265, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI37594469, Length=195, Percent_Identity=29.7435897435897, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI157694516, Length=220, Percent_Identity=27.7272727272727, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI213417737, Length=219, Percent_Identity=27.8538812785388, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI221307494, Length=214, Percent_Identity=28.0373831775701, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI221136756, Length=238, Percent_Identity=25.2100840336134, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI221136753, Length=238, Percent_Identity=25.2100840336134, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI25453481, Length=242, Percent_Identity=25.2066115702479, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI51538573, Length=242, Percent_Identity=25.2066115702479, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI4757966, Length=235, Percent_Identity=25.531914893617, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI51538581, Length=235, Percent_Identity=25.531914893617, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI157694520, Length=185, Percent_Identity=30.2702702702703, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI4757968, Length=242, Percent_Identity=24.7933884297521, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI49355787, Length=242, Percent_Identity=24.7933884297521, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI116235487, Length=177, Percent_Identity=26.5536723163842, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI45643119, Length=214, Percent_Identity=25.2336448598131, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI260275230, Length=214, Percent_Identity=25.2336448598131, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI260274832, Length=214, Percent_Identity=25.2336448598131, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI62530384, Length=233, Percent_Identity=29.6137339055794, Blast_Score=72, Evalue=5e-13,
Organism=Escherichia coli, GI1787659, Length=248, Percent_Identity=41.1290322580645, Blast_Score=177, Evalue=9e-46,
Organism=Escherichia coli, GI221142681, Length=250, Percent_Identity=36, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI1788597, Length=260, Percent_Identity=31.1538461538462, Blast_Score=134, Evalue=9e-33,
Organism=Escherichia coli, GI1788682, Length=209, Percent_Identity=38.2775119617225, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1787660, Length=268, Percent_Identity=33.2089552238806, Blast_Score=117, Evalue=5e-28,
Organism=Escherichia coli, GI87082183, Length=251, Percent_Identity=28.6852589641434, Blast_Score=114, Evalue=6e-27,
Organism=Escherichia coli, GI1790281, Length=193, Percent_Identity=33.6787564766839, Blast_Score=113, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17540714, Length=217, Percent_Identity=47.4654377880184, Blast_Score=210, Evalue=7e-55,
Organism=Caenorhabditis elegans, GI25145438, Length=245, Percent_Identity=43.265306122449, Blast_Score=195, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI17554946, Length=248, Percent_Identity=40.3225806451613, Blast_Score=186, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI17560910, Length=241, Percent_Identity=30.7053941908714, Blast_Score=114, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17534483, Length=228, Percent_Identity=31.140350877193, Blast_Score=112, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17536985, Length=275, Percent_Identity=25.4545454545455, Blast_Score=110, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI17558304, Length=225, Percent_Identity=32, Blast_Score=103, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI17549921, Length=239, Percent_Identity=26.7782426778243, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17535521, Length=206, Percent_Identity=31.0679611650485, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI25144276, Length=234, Percent_Identity=32.0512820512821, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17508953, Length=234, Percent_Identity=32.0512820512821, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17508951, Length=234, Percent_Identity=32.0512820512821, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI25141363, Length=230, Percent_Identity=26.9565217391304, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI25144160, Length=192, Percent_Identity=30.7291666666667, Blast_Score=84, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI25144157, Length=192, Percent_Identity=30.7291666666667, Blast_Score=84, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17540306, Length=249, Percent_Identity=27.710843373494, Blast_Score=77, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6320241, Length=183, Percent_Identity=25.6830601092896, Blast_Score=76, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24653139, Length=248, Percent_Identity=51.6129032258064, Blast_Score=246, Evalue=1e-65,
Organism=Drosophila melanogaster, GI20129971, Length=244, Percent_Identity=41.3934426229508, Blast_Score=182, Evalue=2e-46,
Organism=Drosophila melanogaster, GI24653477, Length=244, Percent_Identity=41.3934426229508, Blast_Score=182, Evalue=2e-46,
Organism=Drosophila melanogaster, GI19920382, Length=228, Percent_Identity=30.2631578947368, Blast_Score=108, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24650670, Length=194, Percent_Identity=35.5670103092784, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI21357171, Length=190, Percent_Identity=31.5789473684211, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI19922422, Length=246, Percent_Identity=26.0162601626016, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24583077, Length=226, Percent_Identity=31.858407079646, Blast_Score=84, Evalue=8e-17,
Organism=Drosophila melanogaster, GI24583079, Length=226, Percent_Identity=31.858407079646, Blast_Score=84, Evalue=8e-17,
Organism=Drosophila melanogaster, GI19921000, Length=226, Percent_Identity=31.858407079646, Blast_Score=84, Evalue=8e-17,
Organism=Drosophila melanogaster, GI28571730, Length=198, Percent_Identity=29.7979797979798, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI28571729, Length=198, Percent_Identity=29.7979797979798, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24654903, Length=173, Percent_Identity=24.8554913294798, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19921018, Length=185, Percent_Identity=29.7297297297297, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24583165, Length=206, Percent_Identity=27.6699029126214, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.55

Molecular weight: Translated: 28465; Mature: 28465

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQLQNISVDYVTPHVVKISLNRERQANSLSLALLEELQNILTQINEEANTRVVILTGAG
CEEECCCCEEEECCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCC
EKAFCAGADLKERAGMNEEQVRHAVSMIRTTMEMVEQLPQPVIAAVNGIALGGGTELCLA
CCEEECCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCEEECCCCCEEEE
CDFRIAAESASLGLTETTLAIIPGAGGTQRLPRLIGVGRAKELIYTGRRISAQEAKEYGL
ECCEEEECCCCCCCEEEEEEEEECCCCHHHHHHHHCCCCHHHHHHCCCCCCHHHHHHCCC
VEFVVPAHLLEEKAIEIAEKIASNGPIAVRLAKEAISNGIQVDLHTGLQMEKQAYEGVIH
CHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHCCCEEEEECCCHHHHHHHCCHHC
TKDRIEGLQAFKEKRTPMYKGE
CHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MLQLQNISVDYVTPHVVKISLNRERQANSLSLALLEELQNILTQINEEANTRVVILTGAG
CEEECCCCEEEECCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCC
EKAFCAGADLKERAGMNEEQVRHAVSMIRTTMEMVEQLPQPVIAAVNGIALGGGTELCLA
CCEEECCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCEEECCCCCEEEE
CDFRIAAESASLGLTETTLAIIPGAGGTQRLPRLIGVGRAKELIYTGRRISAQEAKEYGL
ECCEEEECCCCCCCEEEEEEEEECCCCHHHHHHHHCCCCHHHHHHCCCCCCHHHHHHCCC
VEFVVPAHLLEEKAIEIAEKIASNGPIAVRLAKEAISNGIQVDLHTGLQMEKQAYEGVIH
CHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHCCCEEEEECCCHHHHHHHCCHHC
TKDRIEGLQAFKEKRTPMYKGE
CHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (3R)-3-hydroxybutanoyl-CoA

Specific reaction: (3R)-3-hydroxybutanoyl-CoA = crotonoyl-CoA + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377 [H]