Definition Bacillus cereus E33L, complete genome.
Accession NC_006274
Length 5,300,915

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The map label for this gene is 52142899

Identifier: 52142899

GI number: 52142899

Start: 2474047

End: 2474961

Strand: Direct

Name: 52142899

Synonym: BCZK2342

Alternate gene names: NA

Gene position: 2474047-2474961 (Clockwise)

Preceding gene: 52142901

Following gene: 52142897

Centisome position: 46.67

GC content: 36.61

Gene sequence:

>915_bases
ATGTATCGAATTCATAAAGAACATATCATTTATGCAATGTCACCAGAAAATAAACCATGTATGGAAGTAGAAATTGGGAG
TCGCCTTATATTTGAAACATATGATTGCTTTGAAAATCAAATTGATTCTGAAGATGTTGCGTTTCAAGAATTAGATTGGA
ACCGAATTAATCCGGCGACTGGACCTGTATATATCAATGGTGCAGAGCCTGGTGACATATTAGCGGTAACGATTGAAAAG
ATTCAAATTGCAGAGCAAGGTGTTTTAACTACAGGTATGAATCTTGGTGTAATGGGTGAAGAGCTACATGAAAATACAGT
GAAAATTGTCCCAATACATAATAAACATGTTTTGTTTTCGAATGAACTACAAATCCCAATTAATCCAATGATCGGTGTAA
TTGGAACTGCTCCACAAGAAGAGAGTATTTCATGTGGTACACCGCATGATCACGGCGGGAATATGGACTGTAAAGAGATA
AAAGAAGGGACAACATTATTATTACCTGTAAATGTTCCTGGTGCTCTATTAGCATTAGGTGATTTACACGCAGCGATGGG
TGACGGTGAAATTGGTGTTAGTGGAGTAGAGGTTGCTGGTGAGGTGACGGTAACAGTTCATATTATAAAAGGAAAGAAAT
GGCCATTACCAATGGCTATTCAAAAAGAAAAAATAATGACGCTTGCTTCAGAGAAATTGCTAGATGATGCAGCGAATCGT
GCTGTACGTAATATGGTGACATTTTTACATGAAGAATTACAAATGTCTAAAGCTGATGCCACGCTTTTATTATCAGCTGC
CGGTGATTTAAAAGTTTGTCAAGTTGTGGATCCACTTAAAACAGCACGAATGGAATTAAGTATGGATTATGTGGAGAAGC
TAGGTTTTAATTACAGTAAATGTCATATTAAGTAA

Upstream 100 bases:

>100_bases
AAAGCTTGGTGCTTTTTTACATAAAATTAACATTTCAAGAGGAGCTTGTCTGAAAAAAGCTAATTAGTAATAGTAGATAA
ATATAAAAGGGAGAGGTATT

Downstream 100 bases:

>100_bases
GAATAATAGAAAAAGGATGCTTTTGTATAAAAGTATCCTTTTTAAATTTACGGATTAAGTAAAAAAAGGTATTATTTATT
TTATATAATTGTATATACGA

Product: acetamidase

Products: monocarboxylate; NH3

Alternate protein names: Acetamidase/Formamidase Family Protein; Formamidase; Acetamidase; Amidase; Acetamidase/Formamidase Family; Acetamidase/Formamidase Protein; Amidae; Formamidase/Acetamidase

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MYRIHKEHIIYAMSPENKPCMEVEIGSRLIFETYDCFENQIDSEDVAFQELDWNRINPATGPVYINGAEPGDILAVTIEK
IQIAEQGVLTTGMNLGVMGEELHENTVKIVPIHNKHVLFSNELQIPINPMIGVIGTAPQEESISCGTPHDHGGNMDCKEI
KEGTTLLLPVNVPGALLALGDLHAAMGDGEIGVSGVEVAGEVTVTVHIIKGKKWPLPMAIQKEKIMTLASEKLLDDAANR
AVRNMVTFLHEELQMSKADATLLLSAAGDLKVCQVVDPLKTARMELSMDYVEKLGFNYSKCHIK

Sequences:

>Translated_304_residues
MYRIHKEHIIYAMSPENKPCMEVEIGSRLIFETYDCFENQIDSEDVAFQELDWNRINPATGPVYINGAEPGDILAVTIEK
IQIAEQGVLTTGMNLGVMGEELHENTVKIVPIHNKHVLFSNELQIPINPMIGVIGTAPQEESISCGTPHDHGGNMDCKEI
KEGTTLLLPVNVPGALLALGDLHAAMGDGEIGVSGVEVAGEVTVTVHIIKGKKWPLPMAIQKEKIMTLASEKLLDDAANR
AVRNMVTFLHEELQMSKADATLLLSAAGDLKVCQVVDPLKTARMELSMDYVEKLGFNYSKCHIK
>Mature_304_residues
MYRIHKEHIIYAMSPENKPCMEVEIGSRLIFETYDCFENQIDSEDVAFQELDWNRINPATGPVYINGAEPGDILAVTIEK
IQIAEQGVLTTGMNLGVMGEELHENTVKIVPIHNKHVLFSNELQIPINPMIGVIGTAPQEESISCGTPHDHGGNMDCKEI
KEGTTLLLPVNVPGALLALGDLHAAMGDGEIGVSGVEVAGEVTVTVHIIKGKKWPLPMAIQKEKIMTLASEKLLDDAANR
AVRNMVTFLHEELQMSKADATLLLSAAGDLKVCQVVDPLKTARMELSMDYVEKLGFNYSKCHIK

Specific function: Unknown

COG id: COG2421

COG function: function code C; Predicted acetamidase/formamidase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 3.5.1.4

Molecular weight: Translated: 33338; Mature: 33338

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYRIHKEHIIYAMSPENKPCMEVEIGSRLIFETYDCFENQIDSEDVAFQELDWNRINPAT
CCEEECEEEEEEECCCCCCEEEEECCCEEEEEHHHHHHHCCCCCCCEEEECCCCCCCCCC
GPVYINGAEPGDILAVTIEKIQIAEQGVLTTGMNLGVMGEELHENTVKIVPIHNKHVLFS
CCEEEECCCCCCEEEEEEEEEEHHHCCCEECCCCCCCCCHHHCCCCEEEEEECCCEEEEE
NELQIPINPMIGVIGTAPQEESISCGTPHDHGGNMDCKEIKEGTTLLLPVNVPGALLALG
CCCEECCCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHCCCCEEEEEECCCCCEEEHH
DLHAAMGDGEIGVSGVEVAGEVTVTVHIIKGKKWPLPMAIQKEKIMTLASEKLLDDAANR
HHHHHCCCCCCCCCCEEECCEEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
AVRNMVTFLHEELQMSKADATLLLSAAGDLKVCQVVDPLKTARMELSMDYVEKLGFNYSK
HHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEHHCHHHHHHHHHHHHHHHHHCCCCEE
CHIK
EEEC
>Mature Secondary Structure
MYRIHKEHIIYAMSPENKPCMEVEIGSRLIFETYDCFENQIDSEDVAFQELDWNRINPAT
CCEEECEEEEEEECCCCCCEEEEECCCEEEEEHHHHHHHCCCCCCCEEEECCCCCCCCCC
GPVYINGAEPGDILAVTIEKIQIAEQGVLTTGMNLGVMGEELHENTVKIVPIHNKHVLFS
CCEEEECCCCCCEEEEEEEEEEHHHCCCEECCCCCCCCCHHHCCCCEEEEEECCCEEEEE
NELQIPINPMIGVIGTAPQEESISCGTPHDHGGNMDCKEIKEGTTLLLPVNVPGALLALG
CCCEECCCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHCCCCEEEEEECCCCCEEEHH
DLHAAMGDGEIGVSGVEVAGEVTVTVHIIKGKKWPLPMAIQKEKIMTLASEKLLDDAANR
HHHHHCCCCCCCCCCEEECCEEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
AVRNMVTFLHEELQMSKADATLLLSAAGDLKVCQVVDPLKTARMELSMDYVEKLGFNYSK
HHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEHHCHHHHHHHHHHHHHHHHHCCCCEE
CHIK
EEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: monocarboxylic acid amide; H2O

Specific reaction: A monocarboxylic acid amide + H2O = a monocarboxylate + NH3

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA