Definition Bacillus cereus E33L, complete genome.
Accession NC_006274
Length 5,300,915

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The map label for this gene is lip1 [H]

Identifier: 52142888

GI number: 52142888

Start: 2485818

End: 2487059

Strand: Direct

Name: lip1 [H]

Synonym: BCZK2353

Alternate gene names: 52142888

Gene position: 2485818-2487059 (Clockwise)

Preceding gene: 52142889

Following gene: 52142886

Centisome position: 46.89

GC content: 35.91

Gene sequence:

>1242_bases
ATGGGGAAGTTATTTTTTAAAATATGCTTTTTTGCATTAGTGACTGTTTTTTCATTTGCAGTGAAAATAACGTACGCTGA
AGAGAGTCAACAAAATAATTATCCGATCATTTTAGTAAACGGGTTTGCCGGATGGGGTAGAGAGGAAATGCTAGGGGTTA
AATATTGGGGTGGTGTTCATGATATACAAGAAGATTTGAAACGCAATGGTTATACAGTTCATACCGCAGCTGTTGGACCA
GTTTCTAGTAACTGGGACCGTGCATGTGAATTATATGCCCAAATTAACGGTGGAACAGTAGACTATGGTGCCGCACATGC
TGAGAAACATGGACATAATCGTTTTGGTAGAACTTATAGTGGTTTTGCGCTGAATTGGAGTGAAATAAATAAAGTTCATT
TAGTTGGGCATAGCATGGGTGGACAAACGATTAGAACATTAGTACAGCTATTAAAAGAAGGCAGTTTTGAAGAAAAAAAT
TATGTGAAAAATCACCCAAACACCAAAATATCACCACTATTTGAGGGCGAGAAATCATATGTTCATAGTGTTACAACATT
AGCAACTCCTCATAACGGTACAACACTTGCGGATGGTAGTCTTCTACTACCGTTCGTTAAAGATTTACTCATTACAGCTG
CAAGTTTAGGAGGAAACAGTAATTTATCATTATATGATTTCAAATTGGATCAATGGGGGTTAAAAAAGAATGCTGGAGAG
TCCTTTTTCCAATATACTGATCGTATTCTAAATAGTTCACTTTGGAAAAATACAAAAGATATAAGTCAATGGGATTTAAG
TACAGATGGTGCAAAGGAGTTAAATAATTGGGTAAAGACGCAACCGAATGTGTATTACTTATCCTATAGTGGACATGCAT
CACAAGCAGCACCTATAACGGGTTTACATTTGCCTCATATAACGATGAATAAAGTGTTAATGGGGAATACATTTTTCTTA
GGTTCCTATGCAAGGTATGAAGAAAATCGTCCATTAATTGATACTACTTGGTGGCAAAATGATGGTGTAGTAAATACAAG
TTCTATGATTGCACCTTCTTCTAATGCTACTGTAAATAATAACGAGTCATTACAGATTGGGAAATGGAATCATATCGAAA
CGAAAGCAAATTGGGACCATCTCGACATGGTAGGATTAAGTGTTTCAGACACGTTAGGTTTTTCTAATATTCAAGAGTAT
TATAGAACAATTGCTGAAAAGCTATCACGTTTACCGAAATAG

Upstream 100 bases:

>100_bases
TACAACGTATATAAAATATTTTACATTATGTTTAAGTTAAAAACTGATTGTATCAATTTTTTTCAAGGGAATGAAAGCGT
TTAATTAGGGGGGAGAAAGT

Downstream 100 bases:

>100_bases
ATAATAATAAAGCGCTCTCGATAGAATTCGGGGGCGCTTTATTATATATTAAAAGAGGGATTGGATAGAAAAATATGGGG
GGATATTATGAAAGTTAAAG

Product: lipase

Products: NA

Alternate protein names: Glycerol ester hydrolase 1 [H]

Number of amino acids: Translated: 413; Mature: 412

Protein sequence:

>413_residues
MGKLFFKICFFALVTVFSFAVKITYAEESQQNNYPIILVNGFAGWGREEMLGVKYWGGVHDIQEDLKRNGYTVHTAAVGP
VSSNWDRACELYAQINGGTVDYGAAHAEKHGHNRFGRTYSGFALNWSEINKVHLVGHSMGGQTIRTLVQLLKEGSFEEKN
YVKNHPNTKISPLFEGEKSYVHSVTTLATPHNGTTLADGSLLLPFVKDLLITAASLGGNSNLSLYDFKLDQWGLKKNAGE
SFFQYTDRILNSSLWKNTKDISQWDLSTDGAKELNNWVKTQPNVYYLSYSGHASQAAPITGLHLPHITMNKVLMGNTFFL
GSYARYEENRPLIDTTWWQNDGVVNTSSMIAPSSNATVNNNESLQIGKWNHIETKANWDHLDMVGLSVSDTLGFSNIQEY
YRTIAEKLSRLPK

Sequences:

>Translated_413_residues
MGKLFFKICFFALVTVFSFAVKITYAEESQQNNYPIILVNGFAGWGREEMLGVKYWGGVHDIQEDLKRNGYTVHTAAVGP
VSSNWDRACELYAQINGGTVDYGAAHAEKHGHNRFGRTYSGFALNWSEINKVHLVGHSMGGQTIRTLVQLLKEGSFEEKN
YVKNHPNTKISPLFEGEKSYVHSVTTLATPHNGTTLADGSLLLPFVKDLLITAASLGGNSNLSLYDFKLDQWGLKKNAGE
SFFQYTDRILNSSLWKNTKDISQWDLSTDGAKELNNWVKTQPNVYYLSYSGHASQAAPITGLHLPHITMNKVLMGNTFFL
GSYARYEENRPLIDTTWWQNDGVVNTSSMIAPSSNATVNNNESLQIGKWNHIETKANWDHLDMVGLSVSDTLGFSNIQEY
YRTIAEKLSRLPK
>Mature_412_residues
GKLFFKICFFALVTVFSFAVKITYAEESQQNNYPIILVNGFAGWGREEMLGVKYWGGVHDIQEDLKRNGYTVHTAAVGPV
SSNWDRACELYAQINGGTVDYGAAHAEKHGHNRFGRTYSGFALNWSEINKVHLVGHSMGGQTIRTLVQLLKEGSFEEKNY
VKNHPNTKISPLFEGEKSYVHSVTTLATPHNGTTLADGSLLLPFVKDLLITAASLGGNSNLSLYDFKLDQWGLKKNAGES
FFQYTDRILNSSLWKNTKDISQWDLSTDGAKELNNWVKTQPNVYYLSYSGHASQAAPITGLHLPHITMNKVLMGNTFFLG
SYARYEENRPLIDTTWWQNDGVVNTSSMIAPSSNATVNNNESLQIGKWNHIETKANWDHLDMVGLSVSDTLGFSNIQEYY
RTIAEKLSRLPK

Specific function: Unknown

COG id: COG1075

COG function: function code R; Predicted acetyltransferases and hydrolases with the alpha/beta hydrolase fold

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Lipase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005877 [H]

Pfam domain/function: PF04650 YSIRK_signal [H]

EC number: =3.1.1.3 [H]

Molecular weight: Translated: 46254; Mature: 46123

Theoretical pI: Translated: 7.27; Mature: 7.27

Prosite motif: PS00120 LIPASE_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKLFFKICFFALVTVFSFAVKITYAEESQQNNYPIILVNGFAGWGREEMLGVKYWGGVH
CCHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCEEEEECCCCCCHHHEEEEEECCCHH
DIQEDLKRNGYTVHTAAVGPVSSNWDRACELYAQINGGTVDYGAAHAEKHGHNRFGRTYS
HHHHHHHHCCEEEEEEEECCCCCCHHHHEEEEEEECCCEECCCCHHHHHCCCCCCCCCCC
GFALNWSEINKVHLVGHSMGGQTIRTLVQLLKEGSFEEKNYVKNHPNTKISPLFEGEKSY
CEEEEHHHCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCCCCHHH
VHSVTTLATPHNGTTLADGSLLLPFVKDLLITAASLGGNSNLSLYDFKLDQWGLKKNAGE
HHHHHEEECCCCCCEECCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCH
SFFQYTDRILNSSLWKNTKDISQWDLSTDGAKELNNWVKTQPNVYYLSYSGHASQAAPIT
HHHHHHHHHHHHHHHCCCCCHHHCCCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCCC
GLHLPHITMNKVLMGNTFFLGSYARYEENRPLIDTTWWQNDGVVNTSSMIAPSSNATVNN
CCCCCCEECCCEEECCEEEECCHHHHCCCCCEEEEEEECCCCEECCCCEECCCCCCEECC
NESLQIGKWNHIETKANWDHLDMVGLSVSDTLGFSNIQEYYRTIAEKLSRLPK
CCCEEECCCCCCCCCCCCCCEEEEECCHHHHCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GKLFFKICFFALVTVFSFAVKITYAEESQQNNYPIILVNGFAGWGREEMLGVKYWGGVH
CHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCEEEEECCCCCCHHHEEEEEECCCHH
DIQEDLKRNGYTVHTAAVGPVSSNWDRACELYAQINGGTVDYGAAHAEKHGHNRFGRTYS
HHHHHHHHCCEEEEEEEECCCCCCHHHHEEEEEEECCCEECCCCHHHHHCCCCCCCCCCC
GFALNWSEINKVHLVGHSMGGQTIRTLVQLLKEGSFEEKNYVKNHPNTKISPLFEGEKSY
CEEEEHHHCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCCCCHHH
VHSVTTLATPHNGTTLADGSLLLPFVKDLLITAASLGGNSNLSLYDFKLDQWGLKKNAGE
HHHHHEEECCCCCCEECCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCH
SFFQYTDRILNSSLWKNTKDISQWDLSTDGAKELNNWVKTQPNVYYLSYSGHASQAAPIT
HHHHHHHHHHHHHHHCCCCCHHHCCCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCCC
GLHLPHITMNKVLMGNTFFLGSYARYEENRPLIDTTWWQNDGVVNTSSMIAPSSNATVNN
CCCCCCEECCCEEECCEEEECCHHHHCCCCCEEEEEEECCCCEECCCCEECCCCCCEECC
NESLQIGKWNHIETKANWDHLDMVGLSVSDTLGFSNIQEYYRTIAEKLSRLPK
CCCEEECCCCCCCCCCCCCCEEEEECCHHHHCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA