Click here to switch to the map view.

The map label for this gene is estA [H]

Identifier: 52142767

GI number: 52142767

Start: 2610052

End: 2610915

Strand: Direct

Name: estA [H]

Synonym: BCZK2473

Alternate gene names: 52142767

Gene position: 2610052-2610915 (Clockwise)

Preceding gene: 52142769

Following gene: 52142765

Centisome position: 49.24

GC content: 36.46

Gene sequence:

>864_bases
ATGACCGAAAAGATATTTAAAATAAACGGGATTGATATATGTACAGAAAGTTTTGGGAATCCTAAAAACCCAGCTATTTT
ATTAATTATGGGTGCAACATGTTCAATGATTTATTGGGATGAAGAGTTTTGTGAACGGTTGGCTAACAGCGGTAAATTTG
TTATTCGTTTTGATAACCGTGATGTCGGACGCTCCGTTGCATATGAACCAGGAACTTCCAATTATACCGTTACAGATATG
GCTGAAGATGCAATTGGGGTATTAGATGCTTATCATATTGATAAGGCGCATCTATTTGGTATGTCATTAGGTGGTATGAT
TGCTCAAATCGCTGCTGTAAAACATCCCGAAAGAATTTTAACGTTAACATTACTAGCTACAAGTATCATAGGTTCTGACA
ATAATACTCGTGATTTACCACCGATGGATGAAAGAATTTTAAAACATCACGCAAATGGCACGTATTTAGATTGGACAAAC
GAAAATGTTATAGCAGAATATTTAGTTTCAGGCTCCCGTCTATTATGCGGGTCCAAACGAACATTTGATGAAAAAAGAGT
CTATAATCAAGTCAAACAAGAAATTGAACGAGCTAACAATTTATTAAGCATGTTTAATCACGCTTTACTTCAAGGCGATA
ATGCATATGAAGGGGTGCTTCACTCCATACAAGCACCTACATTAGTCATTCACGGAACAGACGATACTGCACTCCCTTTT
GAACATGGTCTCGCACTTATTGATGAAATTCCTAACTCAGTTCTATTAACCCTTGAAGGGACAGGACATGAAAATCATCC
TGATGATTGGGAAGACATCATTCATGCTGTCACTGAACATACAGCTAAAATAATAGATAAATAA

Upstream 100 bases:

>100_bases
TTTGGGGGTAATTATGAAAAAAACAAAGCAAATCTTATGCACCGTAGAGTCATTCAAAATTATATTTTAAATTAAATTCA
AAAGAAATGGAGAGAACTCT

Downstream 100 bases:

>100_bases
CGAATAAAGGGGCACGCCACAATTTGTGCCCCTTCTATGCGGTACAGTAAAAAGCCCATCACTGTATCCGTATGTATAGT
GATGGGCGATACTTATATTT

Product: carboxylesterase

Products: alcohol; carboxylate

Alternate protein names: Aminohydrolase [H]

Number of amino acids: Translated: 287; Mature: 286

Protein sequence:

>287_residues
MTEKIFKINGIDICTESFGNPKNPAILLIMGATCSMIYWDEEFCERLANSGKFVIRFDNRDVGRSVAYEPGTSNYTVTDM
AEDAIGVLDAYHIDKAHLFGMSLGGMIAQIAAVKHPERILTLTLLATSIIGSDNNTRDLPPMDERILKHHANGTYLDWTN
ENVIAEYLVSGSRLLCGSKRTFDEKRVYNQVKQEIERANNLLSMFNHALLQGDNAYEGVLHSIQAPTLVIHGTDDTALPF
EHGLALIDEIPNSVLLTLEGTGHENHPDDWEDIIHAVTEHTAKIIDK

Sequences:

>Translated_287_residues
MTEKIFKINGIDICTESFGNPKNPAILLIMGATCSMIYWDEEFCERLANSGKFVIRFDNRDVGRSVAYEPGTSNYTVTDM
AEDAIGVLDAYHIDKAHLFGMSLGGMIAQIAAVKHPERILTLTLLATSIIGSDNNTRDLPPMDERILKHHANGTYLDWTN
ENVIAEYLVSGSRLLCGSKRTFDEKRVYNQVKQEIERANNLLSMFNHALLQGDNAYEGVLHSIQAPTLVIHGTDDTALPF
EHGLALIDEIPNSVLLTLEGTGHENHPDDWEDIIHAVTEHTAKIIDK
>Mature_286_residues
TEKIFKINGIDICTESFGNPKNPAILLIMGATCSMIYWDEEFCERLANSGKFVIRFDNRDVGRSVAYEPGTSNYTVTDMA
EDAIGVLDAYHIDKAHLFGMSLGGMIAQIAAVKHPERILTLTLLATSIIGSDNNTRDLPPMDERILKHHANGTYLDWTNE
NVIAEYLVSGSRLLCGSKRTFDEKRVYNQVKQEIERANNLLSMFNHALLQGDNAYEGVLHSIQAPTLVIHGTDDTALPFE
HGLALIDEIPNSVLLTLEGTGHENHPDDWEDIIHAVTEHTAKIIDK

Specific function: May increase the rate of spontaneous hydrolysis of aminoacrylate to malonic semialdehyde. Required to remove a toxic intermediate produce in the pyrimidine nitrogen degradation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Hydrolase RutD family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR019913 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.1.1.1

Molecular weight: Translated: 31947; Mature: 31815

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEKIFKINGIDICTESFGNPKNPAILLIMGATCSMIYWDEEFCERLANSGKFVIRFDNR
CCCCEEEECCCEEEHHHCCCCCCCEEEEEECCCEEEEEECHHHHHHHHCCCCEEEEECCC
DVGRSVAYEPGTSNYTVTDMAEDAIGVLDAYHIDKAHLFGMSLGGMIAQIAAVKHPERIL
CCCCEEEECCCCCCEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHH
TLTLLATSIIGSDNNTRDLPPMDERILKHHANGTYLDWTNENVIAEYLVSGSRLLCGSKR
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCEEEECCCHHHHHHHHCCCEEEECCCC
TFDEKRVYNQVKQEIERANNLLSMFNHALLQGDNAYEGVLHSIQAPTLVIHGTDDTALPF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEECCCCCCCCH
EHGLALIDEIPNSVLLTLEGTGHENHPDDWEDIIHAVTEHTAKIIDK
HHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TEKIFKINGIDICTESFGNPKNPAILLIMGATCSMIYWDEEFCERLANSGKFVIRFDNR
CCCEEEECCCEEEHHHCCCCCCCEEEEEECCCEEEEEECHHHHHHHHCCCCEEEEECCC
DVGRSVAYEPGTSNYTVTDMAEDAIGVLDAYHIDKAHLFGMSLGGMIAQIAAVKHPERIL
CCCCEEEECCCCCCEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHH
TLTLLATSIIGSDNNTRDLPPMDERILKHHANGTYLDWTNENVIAEYLVSGSRLLCGSKR
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCEEEECCCHHHHHHHHCCCEEEECCCC
TFDEKRVYNQVKQEIERANNLLSMFNHALLQGDNAYEGVLHSIQAPTLVIHGTDDTALPF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEECCCCCCCCH
EHGLALIDEIPNSVLLTLEGTGHENHPDDWEDIIHAVTEHTAKIIDK
HHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: carboxylic ester; H2O

Specific reaction: A carboxylic ester + H2O = an alcohol + a carboxylate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA