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The map label for this gene is miaA

Identifier: 52141773

GI number: 52141773

Start: 3597902

End: 3598855

Strand: Reverse

Name: miaA

Synonym: BCZK3473

Alternate gene names: 52141773

Gene position: 3598855-3597902 (Counterclockwise)

Preceding gene: 52141775

Following gene: 52141772

Centisome position: 67.89

GC content: 36.79

Gene sequence:

>954_bases
ATGGGAGAAGTGCAACGTGAAAAAGTTGCTGTCATCATTGGACCAACTGCTGTTGGAAAAACGAAGTTAAGTATTGATCT
TGCTAAAGCGTTGAACGGTGAAATTATAAGTGGAGATTCCATGCAGATTTATCGTACGATGGATATTGGAACTGCAAAGG
TGACGAAAGAAGAGATGGATGGAATTCCACATTATATGGTCGATATAAAAAATCCGGAAGAATCATTTTCAGTTGCCGAA
TTTCAAGAACGTGTTCGTAAGCATATTCGAGAGATTACAGAGCGTGGTAAATTACCAATTATCGTTGGTGGAACTGGTCT
GTATATACAGTCTGTTTTATTCGATTATCAGTTTACGGATGATGCTGGTGATGCTATATACCGAGAACAAATGGAAAAGT
TAGCATTAGAACGTGGTGTGGAATATGTACATAAGAAATTACAAGAAGTAGACCCAGAAAGCGCTGAGCGTATTCATGCA
AATAATGTGCGTCGTGTCATTAGAGCGTTAGAAATTTTTCACACGTCGGGTGAAAAAATGAGTGATCAACTTGAAAAACA
AGAAAACGAGTTATTATACGATGTTTCCTTAATTGGCTTGACAATGGATCGTGAAATGCTATACGATCGCATTAACTTAC
GAGTTGATATAATGATGGACCAAGGTTTATTAGAAGAAGTAGAAGGATTGTATAATAGAGGGATACGAGATTGTCAATCT
ATTCAAGCGATTGGGTATAAAGAGATATATGATTATTTTGAGGATCGTGTCTCTTTAGAAGAAGCGGTATCACAATTAAA
GACGAATTCACGTCGTTATGCAAAACGTCAATTAACGTGGTTCCGTAATAAGATGGATGTCACATGGTTTGATGTTACAG
ACGGTGAAAAAACGTCAGAAATTTTACGATACATAGAAGGAAAGCTACAACTAAAGTCGAATAATAGTAAGTAG

Upstream 100 bases:

>100_bases
CATGTACCGTAATGAAAGAACTTGCAATCATGCTGTTAAAATTGGTAAGATTGGATATAATCAATAGATGGAGTACATTA
CATAAAGAGAGTTGAATGAT

Downstream 100 bases:

>100_bases
AGAAAAAGAGGAGGATTCGACATGAAGCAATCAATCAATATTCAAGATCAGTTTTTAAATCAACTCCGTAAAGAGAATAC
GTTTGTTACGCTGTACTTAT

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 317; Mature: 316

Protein sequence:

>317_residues
MGEVQREKVAVIIGPTAVGKTKLSIDLAKALNGEIISGDSMQIYRTMDIGTAKVTKEEMDGIPHYMVDIKNPEESFSVAE
FQERVRKHIREITERGKLPIIVGGTGLYIQSVLFDYQFTDDAGDAIYREQMEKLALERGVEYVHKKLQEVDPESAERIHA
NNVRRVIRALEIFHTSGEKMSDQLEKQENELLYDVSLIGLTMDREMLYDRINLRVDIMMDQGLLEEVEGLYNRGIRDCQS
IQAIGYKEIYDYFEDRVSLEEAVSQLKTNSRRYAKRQLTWFRNKMDVTWFDVTDGEKTSEILRYIEGKLQLKSNNSK

Sequences:

>Translated_317_residues
MGEVQREKVAVIIGPTAVGKTKLSIDLAKALNGEIISGDSMQIYRTMDIGTAKVTKEEMDGIPHYMVDIKNPEESFSVAE
FQERVRKHIREITERGKLPIIVGGTGLYIQSVLFDYQFTDDAGDAIYREQMEKLALERGVEYVHKKLQEVDPESAERIHA
NNVRRVIRALEIFHTSGEKMSDQLEKQENELLYDVSLIGLTMDREMLYDRINLRVDIMMDQGLLEEVEGLYNRGIRDCQS
IQAIGYKEIYDYFEDRVSLEEAVSQLKTNSRRYAKRQLTWFRNKMDVTWFDVTDGEKTSEILRYIEGKLQLKSNNSK
>Mature_316_residues
GEVQREKVAVIIGPTAVGKTKLSIDLAKALNGEIISGDSMQIYRTMDIGTAKVTKEEMDGIPHYMVDIKNPEESFSVAEF
QERVRKHIREITERGKLPIIVGGTGLYIQSVLFDYQFTDDAGDAIYREQMEKLALERGVEYVHKKLQEVDPESAERIHAN
NVRRVIRALEIFHTSGEKMSDQLEKQENELLYDVSLIGLTMDREMLYDRINLRVDIMMDQGLLEEVEGLYNRGIRDCQSI
QAIGYKEIYDYFEDRVSLEEAVSQLKTNSRRYAKRQLTWFRNKMDVTWFDVTDGEKTSEILRYIEGKLQLKSNNSK

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=314, Percent_Identity=31.8471337579618, Blast_Score=155, Evalue=3e-38,
Organism=Escherichia coli, GI1790613, Length=302, Percent_Identity=38.4105960264901, Blast_Score=216, Evalue=2e-57,
Organism=Caenorhabditis elegans, GI25144712, Length=307, Percent_Identity=32.8990228013029, Blast_Score=135, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6324848, Length=307, Percent_Identity=34.2019543973941, Blast_Score=179, Evalue=4e-46,
Organism=Drosophila melanogaster, GI24649830, Length=341, Percent_Identity=31.3782991202346, Blast_Score=167, Evalue=7e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_BACAH (A0RH88)

Other databases:

- EMBL:   CP000485
- RefSeq:   YP_896088.1
- ProteinModelPortal:   A0RH88
- SMR:   A0RH88
- STRING:   A0RH88
- EnsemblBacteria:   EBBACT00000069290
- GeneID:   4544978
- GenomeReviews:   CP000485_GR
- KEGG:   btl:BALH_3341
- eggNOG:   COG0324
- GeneTree:   EBGT00050000001497
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- ProtClustDB:   PRK00091
- BioCyc:   BTHU412694:BALH_3341-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 36652; Mature: 36521

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGEVQREKVAVIIGPTAVGKTKLSIDLAKALNGEIISGDSMQIYRTMDIGTAKVTKEEMD
CCCCCCCCEEEEECCCCCCCEEHHEEHHHHCCCCEECCCCEEEEEEECCCCHHCCHHHHC
GIPHYMVDIKNPEESFSVAEFQERVRKHIREITERGKLPIIVGGTGLYIQSVLFDYQFTD
CCCEEEEEECCCHHHCCHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHCEECC
DAGDAIYREQMEKLALERGVEYVHKKLQEVDPESAERIHANNVRRVIRALEIFHTSGEKM
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHH
SDQLEKQENELLYDVSLIGLTMDREMLYDRINLRVDIMMDQGLLEEVEGLYNRGIRDCQS
HHHHHHHHHHEEEEHEEHEEEHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHCCHHHHHH
IQAIGYKEIYDYFEDRVSLEEAVSQLKTNSRRYAKRQLTWFRNKMDVTWFDVTDGEKTSE
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHH
ILRYIEGKLQLKSNNSK
HHHHHCCCEEEECCCCC
>Mature Secondary Structure 
GEVQREKVAVIIGPTAVGKTKLSIDLAKALNGEIISGDSMQIYRTMDIGTAKVTKEEMD
CCCCCCCEEEEECCCCCCCEEHHEEHHHHCCCCEECCCCEEEEEEECCCCHHCCHHHHC
GIPHYMVDIKNPEESFSVAEFQERVRKHIREITERGKLPIIVGGTGLYIQSVLFDYQFTD
CCCEEEEEECCCHHHCCHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHCEECC
DAGDAIYREQMEKLALERGVEYVHKKLQEVDPESAERIHANNVRRVIRALEIFHTSGEKM
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHH
SDQLEKQENELLYDVSLIGLTMDREMLYDRINLRVDIMMDQGLLEEVEGLYNRGIRDCQS
HHHHHHHHHHEEEEHEEHEEEHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHCCHHHHHH
IQAIGYKEIYDYFEDRVSLEEAVSQLKTNSRRYAKRQLTWFRNKMDVTWFDVTDGEKTSE
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHH
ILRYIEGKLQLKSNNSK
HHHHHCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA