Click here to switch to the map view.

The map label for this gene is nusA [H]

Identifier: 52141674

GI number: 52141674

Start: 3708016

End: 3709122

Strand: Reverse

Name: nusA [H]

Synonym: BCZK3575

Alternate gene names: 52141674

Gene position: 3709122-3708016 (Counterclockwise)

Preceding gene: 52141671

Following gene: 52141673

Centisome position: 69.97

GC content: 38.75

Gene sequence:

>1107_bases
ATGAGCACTGAGTTGTTAGATGCCTTGCTCGTATTAGAGTCAGAAAAAGGAATTAGCAAAGATATTATTATTGATGCGAT
TGAAGCAGCGTTAATCTCTGCTTATAAACGCAATTTTAACCAAGCACAAAACGTTCGTGTGAGCTTTAACCCACAAGTGG
GAACAATCCAAGTTTTAGCACGTAAAGACGTTGTTGATAATGTGTTTGATCCACGTCTTGAAATTTCTGTTGAAGAAGCA
AGACAAATTAATCCAAACTATCAAGATGGTGACGTACTAGAAATTGAAGTAACACCAAAAGATTTCGGTCGTATTGCAGC
ACAAACTGCAAAACAAGTTGTAACACAACGAGTGCGTGAAGCTGAGCGTGGTGTTATTTACTCTGAGTTTAGTGACCGTG
AAGAAGATATCATGGTTGGTATCGTACAACGTCAAGATGCTCGTTTCATCTATGTAAGCTTAGGGAAAGTAGAAGCTTTA
TTACCTGTAAGTGAGCAAATGCCAAACGAACAATACAAACCACATGATCGTATTCGCGTATTCATTACGAAAGTAGAAAA
AACAACAAAAGGACCACAAATTTACGTATCACGTACACATCCTGGTCTTTTAAAACGTTTATTCGAAATGGAAGTTCCTG
AAATCTATGATGGAACGGTTGAAATTCGTTCTGTAGCACGTGAAGCAGGAGATCGTTCTAAAATCTCTGTACATGCAGAG
AACATTGATGTTGATCCAGTAGGTTCTTGTGTAGGACCGAAAGGACAACGTGTACAACGCATTGTAGACGAACTAAAAGG
TGAAAAGATTGACATCGTTCGCTGGTCAAATGATCCAGTAGAATATGTTGCCAATGCTTTAAGCCCATCACAAGTTGTGA
AAGTACTTGTAGATGAAGAAGAAAAAGCAACAACTGTTGTTGTTCCAGACCACCAACTGTCATTAGCTATCGGTAAACGT
GGACAAAATGCACGTCTTGCAGCTAAATTAACAGGTTGGAAAATTGATATTAAGAGTGAGTCTGATGCGAAACAACTTGG
TATTGTAACAGAAGAAGATAGCGTAGTCGCATTCGGATTCGATTCTGTTGAAGACGAAATCGAATAG

Upstream 100 bases:

>100_bases
TATTAACAATTAAAACACGTAAAAAAGAAATCCAAATTCCAATGGACAAAGTTGCAAATGCGCGACTTGCTGTTACGTTT
TAGTTAGAAGGGGGATCTTC

Downstream 100 bases:

>100_bases
AGGTGATGAGTATGAGCAATCGAAAAGTTCCGTTACGAAAATGTGTTGCAACGCAAGAAATGAAATCAAAACGAGAGCTC
GTTCGCATTGTTCGTTCCAA

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 368; Mature: 367

Protein sequence:

>368_residues
MSTELLDALLVLESEKGISKDIIIDAIEAALISAYKRNFNQAQNVRVSFNPQVGTIQVLARKDVVDNVFDPRLEISVEEA
RQINPNYQDGDVLEIEVTPKDFGRIAAQTAKQVVTQRVREAERGVIYSEFSDREEDIMVGIVQRQDARFIYVSLGKVEAL
LPVSEQMPNEQYKPHDRIRVFITKVEKTTKGPQIYVSRTHPGLLKRLFEMEVPEIYDGTVEIRSVAREAGDRSKISVHAE
NIDVDPVGSCVGPKGQRVQRIVDELKGEKIDIVRWSNDPVEYVANALSPSQVVKVLVDEEEKATTVVVPDHQLSLAIGKR
GQNARLAAKLTGWKIDIKSESDAKQLGIVTEEDSVVAFGFDSVEDEIE

Sequences:

>Translated_368_residues
MSTELLDALLVLESEKGISKDIIIDAIEAALISAYKRNFNQAQNVRVSFNPQVGTIQVLARKDVVDNVFDPRLEISVEEA
RQINPNYQDGDVLEIEVTPKDFGRIAAQTAKQVVTQRVREAERGVIYSEFSDREEDIMVGIVQRQDARFIYVSLGKVEAL
LPVSEQMPNEQYKPHDRIRVFITKVEKTTKGPQIYVSRTHPGLLKRLFEMEVPEIYDGTVEIRSVAREAGDRSKISVHAE
NIDVDPVGSCVGPKGQRVQRIVDELKGEKIDIVRWSNDPVEYVANALSPSQVVKVLVDEEEKATTVVVPDHQLSLAIGKR
GQNARLAAKLTGWKIDIKSESDAKQLGIVTEEDSVVAFGFDSVEDEIE
>Mature_367_residues
STELLDALLVLESEKGISKDIIIDAIEAALISAYKRNFNQAQNVRVSFNPQVGTIQVLARKDVVDNVFDPRLEISVEEAR
QINPNYQDGDVLEIEVTPKDFGRIAAQTAKQVVTQRVREAERGVIYSEFSDREEDIMVGIVQRQDARFIYVSLGKVEALL
PVSEQMPNEQYKPHDRIRVFITKVEKTTKGPQIYVSRTHPGLLKRLFEMEVPEIYDGTVEIRSVAREAGDRSKISVHAEN
IDVDPVGSCVGPKGQRVQRIVDELKGEKIDIVRWSNDPVEYVANALSPSQVVKVLVDEEEKATTVVVPDHQLSLAIGKRG
QNARLAAKLTGWKIDIKSESDAKQLGIVTEEDSVVAFGFDSVEDEIE

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=343, Percent_Identity=37.9008746355685, Blast_Score=258, Evalue=4e-70,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 41150; Mature: 41019

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTELLDALLVLESEKGISKDIIIDAIEAALISAYKRNFNQAQNVRVSFNPQVGTIQVLA
CCHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHCCCHHHCEEEEECCCCCEEEEEE
RKDVVDNVFDPRLEISVEEARQINPNYQDGDVLEIEVTPKDFGRIAAQTAKQVVTQRVRE
HHHHHHHHCCCCEEEEHHHHHCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHH
AERGVIYSEFSDREEDIMVGIVQRQDARFIYVSLGKVEALLPVSEQMPNEQYKPHDRIRV
HHCCEEEECCCCCCCCEEEEEEECCCCCEEEEEECCEEEECCCHHHCCCCCCCCCCCEEE
FITKVEKTTKGPQIYVSRTHPGLLKRLFEMEVPEIYDGTVEIRSVAREAGDRSKISVHAE
EEEEEHHCCCCCEEEEECCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEC
NIDVDPVGSCVGPKGQRVQRIVDELKGEKIDIVRWSNDPVEYVANALSPSQVVKVLVDEE
CCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHCCCHHHHHHHEECCC
EKATTVVVPDHQLSLAIGKRGQNARLAAKLTGWKIDIKSESDAKQLGIVTEEDSVVAFGF
CCCEEEEECCCEEEEEECCCCCCCEEEEEEECEEEEECCCCCHHHCCEEECCCCEEEEEC
DSVEDEIE
CCCHHCCC
>Mature Secondary Structure 
STELLDALLVLESEKGISKDIIIDAIEAALISAYKRNFNQAQNVRVSFNPQVGTIQVLA
CHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHCCCHHHCEEEEECCCCCEEEEEE
RKDVVDNVFDPRLEISVEEARQINPNYQDGDVLEIEVTPKDFGRIAAQTAKQVVTQRVRE
HHHHHHHHCCCCEEEEHHHHHCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHH
AERGVIYSEFSDREEDIMVGIVQRQDARFIYVSLGKVEALLPVSEQMPNEQYKPHDRIRV
HHCCEEEECCCCCCCCEEEEEEECCCCCEEEEEECCEEEECCCHHHCCCCCCCCCCCEEE
FITKVEKTTKGPQIYVSRTHPGLLKRLFEMEVPEIYDGTVEIRSVAREAGDRSKISVHAE
EEEEEHHCCCCCEEEEECCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEC
NIDVDPVGSCVGPKGQRVQRIVDELKGEKIDIVRWSNDPVEYVANALSPSQVVKVLVDEE
CCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHCCCHHHHHHHEECCC
EKATTVVVPDHQLSLAIGKRGQNARLAAKLTGWKIDIKSESDAKQLGIVTEEDSVVAFGF
CCCEEEEECCCEEEEEECCCCCCCEEEEEEECEEEEECCCCCHHHCCEEECCCCEEEEEC
DSVEDEIE
CCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]