Definition Bacillus cereus E33L, complete genome.
Accession NC_006274
Length 5,300,915

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The map label for this gene is glpT [H]

Identifier: 52140305

GI number: 52140305

Start: 5048461

End: 5049795

Strand: Reverse

Name: glpT [H]

Synonym: BCZK4955

Alternate gene names: 52140305

Gene position: 5049795-5048461 (Counterclockwise)

Preceding gene: 52140304

Following gene: 52140308

Centisome position: 95.26

GC content: 37.38

Gene sequence:

>1335_bases
ATGTTTAAATGGCTCAAGCCAGCACCTGCAATTGAAAGATTGCCTGCGGATATGATCGACCGTGTGTATCGATTACTACG
TATTCGTGTGTTAATCGGAATTTCAGTTGGATATGCTGCTTATTATTTAGTTCGTAGTAACTTTACGTTATCAAGTACGT
ACTTAGTACAAGAATATGGTTTTAGTACAGCTGAAATTGGTCTACTAGGTTCAGTAATGGCAATTGTTTATGGTTTTAGT
AAGTTCTTTATGGGGAATTTATCAGATAAAGCTTTCGCCCAGCGCTTTATCGCAGTCGGTTTATTTTTATCAGGGCTTGT
AAATATTTGCTTCGGTTTTGCATCTTCATTTGGGATGATTGTTACATTACTTGTCGTTAACGGTATTGTACAAGGTATGG
GAGCACCACCTTGTAGTATTGTTATGACGAAATGGTTCTCGAAGAAAGAGCGCGGTACGAAAACAGGTATTTGGAATATT
TCACATAACGTTGGTGGAATGCTTGTACCACCACTTGTTGGGATTGGTGTAGGTATTTTTGGTGAAAATCATTGGCAAGG
CGGCGTGTTTATTTTCCCGGCGATTATCGCAATGATAATTTCAGTTCTTGTTTGGATTAATGCGAAGGATACACCGGAAT
CAGAAGGTCTTCCTCCAATTGATGAGTATCGTAATGACTATGAAAATCTTGAAAAAGCAGACAACGCTAACAAGATGTCA
CCAAAAGAAATTTTAATGAAGTATGTAGTGAAAAATAAATTTGTATGGTTCTTATGTATTGCAAATGCATTTGTATATTT
AATTCGCTTCGGTGTTATTAACTGGGTTCCACTGTATTTAACGACAGTTAAAGGTTTCTCAAAAAATGAAGCACACGCGG
CGTACGCAATCTTTGAAGGTATGGCAATTCCAAGTTCATTAATCGTTGGTCTTTTAAGTGATAAATTATTTAAAGGAAAA
CGTATGCCATTATGTATCATTAGTATGGTCGGAGTTGTTATTGGTACGTTTGTATATTGGCAAGCAACTAGCATACTTGT
TGTAAGTATTGCAGTTTCTATTATCGGTTGTTTAATTTATGTACCACAGTTCTTAATCGGTTTAAGTGCGATGGAATTAG
TACCGAAATTTGCAGTAGGTACGACAGTTGGTATGTGTGGTCTGTTCGGTTATGTAGGCGGAAGTCTTGTAGCAAACGCA
GCAATCGGTGTTATTGTTGATCGTTCTGGCTGGGATGGTTGTTTCATCTTACTATTAACAGGTGCCATTTTATCAACAAT
CTTCTTGTTCATCGTTCAACGTGGACATGAGAGCAAAGCTTCTAAAGTAGCATAA

Upstream 100 bases:

>100_bases
CCGGTTAGAAGATCAAACCAAAATATATCAAATAGCGGAATAATCGAGATATGGAATAGACAACGTCTCTCTCAGAAGTG
AGAAAAGGAGACGAAAGAAT

Downstream 100 bases:

>100_bases
TATTTCATTTTAAAAAGCTATCCAGTTTTTATACTGGATAGCTTTTATATTTTTATACGAGGAAAATCAGTATGTAAAAT
TGCAGTTTAATATGTAAATG

Product: glycerol-3-phosphate transporter

Products: Proton [Cytoplasm]; glycerol-3-phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
MFKWLKPAPAIERLPADMIDRVYRLLRIRVLIGISVGYAAYYLVRSNFTLSSTYLVQEYGFSTAEIGLLGSVMAIVYGFS
KFFMGNLSDKAFAQRFIAVGLFLSGLVNICFGFASSFGMIVTLLVVNGIVQGMGAPPCSIVMTKWFSKKERGTKTGIWNI
SHNVGGMLVPPLVGIGVGIFGENHWQGGVFIFPAIIAMIISVLVWINAKDTPESEGLPPIDEYRNDYENLEKADNANKMS
PKEILMKYVVKNKFVWFLCIANAFVYLIRFGVINWVPLYLTTVKGFSKNEAHAAYAIFEGMAIPSSLIVGLLSDKLFKGK
RMPLCIISMVGVVIGTFVYWQATSILVVSIAVSIIGCLIYVPQFLIGLSAMELVPKFAVGTTVGMCGLFGYVGGSLVANA
AIGVIVDRSGWDGCFILLLTGAILSTIFLFIVQRGHESKASKVA

Sequences:

>Translated_444_residues
MFKWLKPAPAIERLPADMIDRVYRLLRIRVLIGISVGYAAYYLVRSNFTLSSTYLVQEYGFSTAEIGLLGSVMAIVYGFS
KFFMGNLSDKAFAQRFIAVGLFLSGLVNICFGFASSFGMIVTLLVVNGIVQGMGAPPCSIVMTKWFSKKERGTKTGIWNI
SHNVGGMLVPPLVGIGVGIFGENHWQGGVFIFPAIIAMIISVLVWINAKDTPESEGLPPIDEYRNDYENLEKADNANKMS
PKEILMKYVVKNKFVWFLCIANAFVYLIRFGVINWVPLYLTTVKGFSKNEAHAAYAIFEGMAIPSSLIVGLLSDKLFKGK
RMPLCIISMVGVVIGTFVYWQATSILVVSIAVSIIGCLIYVPQFLIGLSAMELVPKFAVGTTVGMCGLFGYVGGSLVANA
AIGVIVDRSGWDGCFILLLTGAILSTIFLFIVQRGHESKASKVA
>Mature_444_residues
MFKWLKPAPAIERLPADMIDRVYRLLRIRVLIGISVGYAAYYLVRSNFTLSSTYLVQEYGFSTAEIGLLGSVMAIVYGFS
KFFMGNLSDKAFAQRFIAVGLFLSGLVNICFGFASSFGMIVTLLVVNGIVQGMGAPPCSIVMTKWFSKKERGTKTGIWNI
SHNVGGMLVPPLVGIGVGIFGENHWQGGVFIFPAIIAMIISVLVWINAKDTPESEGLPPIDEYRNDYENLEKADNANKMS
PKEILMKYVVKNKFVWFLCIANAFVYLIRFGVINWVPLYLTTVKGFSKNEAHAAYAIFEGMAIPSSLIVGLLSDKLFKGK
RMPLCIISMVGVVIGTFVYWQATSILVVSIAVSIIGCLIYVPQFLIGLSAMELVPKFAVGTTVGMCGLFGYVGGSLVANA
AIGVIVDRSGWDGCFILLLTGAILSTIFLFIVQRGHESKASKVA

Specific function: The phosphoglycerate transporter protein is a part of the PGT transport system. It is the membrane bound transporter for phosphoglycerate into salmonella [H]

COG id: COG2271

COG function: function code G; Sugar phosphate permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Organophosphate:Pi antiporter (OPA) (TC 2.A.1.4) family [H]

Homologues:

Organism=Homo sapiens, GI256219598, Length=441, Percent_Identity=24.7165532879819, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI4503847, Length=441, Percent_Identity=24.7165532879819, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI256219483, Length=441, Percent_Identity=24.7165532879819, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI256219543, Length=463, Percent_Identity=23.9740820734341, Blast_Score=115, Evalue=6e-26,
Organism=Homo sapiens, GI223717983, Length=398, Percent_Identity=24.1206030150754, Blast_Score=92, Evalue=9e-19,
Organism=Homo sapiens, GI38093649, Length=398, Percent_Identity=24.1206030150754, Blast_Score=92, Evalue=9e-19,
Organism=Homo sapiens, GI256219564, Length=366, Percent_Identity=23.7704918032787, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI46361976, Length=425, Percent_Identity=24.2352941176471, Blast_Score=69, Evalue=8e-12,
Organism=Escherichia coli, GI1788573, Length=449, Percent_Identity=43.4298440979955, Blast_Score=371, Evalue=1e-104,
Organism=Escherichia coli, GI87082310, Length=433, Percent_Identity=33.4872979214781, Blast_Score=248, Evalue=4e-67,
Organism=Escherichia coli, GI2367259, Length=449, Percent_Identity=30.2895322939866, Blast_Score=199, Evalue=2e-52,
Organism=Escherichia coli, GI2367379, Length=361, Percent_Identity=24.3767313019391, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1789515, Length=446, Percent_Identity=21.9730941704036, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI87082320, Length=263, Percent_Identity=25.4752851711027, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI1789152, Length=413, Percent_Identity=22.2760290556901, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17564204, Length=375, Percent_Identity=25.6, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17542316, Length=410, Percent_Identity=22.9268292682927, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17564202, Length=370, Percent_Identity=25.6756756756757, Blast_Score=73, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI133930953, Length=301, Percent_Identity=23.5880398671096, Blast_Score=69, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24656866, Length=492, Percent_Identity=20.9349593495935, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI45550478, Length=492, Percent_Identity=20.9349593495935, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24656861, Length=492, Percent_Identity=20.9349593495935, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000849
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR021159 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 48463; Mature: 48463

Theoretical pI: Translated: 9.44; Mature: 9.44

Prosite motif: PS50850 MFS ; PS00942 GLPT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; glycerol-3-phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + glycerol-3-phosphate [Periplasm] = Proton [Cytoplasm] + glycerol-3-phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677609; 3042749 [H]