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The map label for this gene is cwlJ [H]

Identifier: 52140175

GI number: 52140175

Start: 5198667

End: 5199089

Strand: Direct

Name: cwlJ [H]

Synonym: BCZK5088

Alternate gene names: 52140175

Gene position: 5198667-5199089 (Clockwise)

Preceding gene: 52140174

Following gene: 52140170

Centisome position: 98.07

GC content: 43.74

Gene sequence:

>423_bases
ATGGGCGTTATCGCGTATAACGAAGAAGATGTAAAGTTATTAGCTAGACTGATGCGTGCTGAAGCTGAAGGAGAAGGTCA
ACAGGGAATGTTGATGGTCGGAAACGTTGGAGTAAATCGTGTCCGCGGAAATTGTTTAGATTTTAAAAGTATTCGTAATT
TACGTCAAATGGTCTATCAAAACCCTGGCGGTTTTGAAGCAACACAAAAAGGTTATTTTTATCAGCGTGCGAGAGAACAA
GATATCGCCTTAGCAAGGCGAACGATTCAAGGACAACGTTTCTGGCCTGCCAACTTTGCCTTATGGTTTTTTAGGCCTGA
AGGTGCATGTCCACCAACTTGGTATAACCAGCAAAACTCTGGTCGCTTCAAGAAGCACTGCTTCTTCCAGCCATCTGCTG
GGGATTGCCCTAGCGTATATTAA

Upstream 100 bases:

>100_bases
GAGTCCTTTTTCTCATACGTATGAACTAGTAATTGTTACACACTTCATCTAGAGATAAACCTCTTTCCACAAGGTATTCC
AAAAAGAGAGGTGACACATA

Downstream 100 bases:

>100_bases
GGGATGAAAACAAGGAGGGATTTTTGAATGGCACAGCAACAAAACCCGTATTATGGAACAGGATTTTATCAACCATCTGG
AACTTATGTACAACCGCAAC

Product: cell wall hydrolase; cortex lytic enzyme

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 140; Mature: 139

Protein sequence:

>140_residues
MGVIAYNEEDVKLLARLMRAEAEGEGQQGMLMVGNVGVNRVRGNCLDFKSIRNLRQMVYQNPGGFEATQKGYFYQRAREQ
DIALARRTIQGQRFWPANFALWFFRPEGACPPTWYNQQNSGRFKKHCFFQPSAGDCPSVY

Sequences:

>Translated_140_residues
MGVIAYNEEDVKLLARLMRAEAEGEGQQGMLMVGNVGVNRVRGNCLDFKSIRNLRQMVYQNPGGFEATQKGYFYQRAREQ
DIALARRTIQGQRFWPANFALWFFRPEGACPPTWYNQQNSGRFKKHCFFQPSAGDCPSVY
>Mature_139_residues
GVIAYNEEDVKLLARLMRAEAEGEGQQGMLMVGNVGVNRVRGNCLDFKSIRNLRQMVYQNPGGFEATQKGYFYQRAREQD
IALARRTIQGQRFWPANFALWFFRPEGACPPTWYNQQNSGRFKKHCFFQPSAGDCPSVY

Specific function: Probable spore cortex-lytic enzyme involved in the depolymerization of cortex peptidoglycan during germination [H]

COG id: COG3773

COG function: function code M; Cell wall hydrolyses involved in spore germination

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CwlJ family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011105 [H]

Pfam domain/function: PF07486 Hydrolase_2 [H]

EC number: 3.5.1.28

Molecular weight: Translated: 16097; Mature: 15965

Theoretical pI: Translated: 9.51; Mature: 9.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVIAYNEEDVKLLARLMRAEAEGEGQQGMLMVGNVGVNRVRGNCLDFKSIRNLRQMVYQ
CCEEECCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHC
NPGGFEATQKGYFYQRAREQDIALARRTIQGQRFWPANFALWFFRPEGACPPTWYNQQNS
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEECCCCEEEEEECCCCCCCCCCCCCCCC
GRFKKHCFFQPSAGDCPSVY
CCCCCEEEECCCCCCCCCCC
>Mature Secondary Structure 
GVIAYNEEDVKLLARLMRAEAEGEGQQGMLMVGNVGVNRVRGNCLDFKSIRNLRQMVYQ
CEEECCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHC
NPGGFEATQKGYFYQRAREQDIALARRTIQGQRFWPANFALWFFRPEGACPPTWYNQQNS
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEECCCCEEEEEECCCCCCCCCCCCCCCC
GRFKKHCFFQPSAGDCPSVY
CCCCCEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376 [H]