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The map label for this gene is dacA [H]

Identifier: 52140174

GI number: 52140174

Start: 5197220

End: 5198476

Strand: Direct

Name: dacA [H]

Synonym: BCZK5087

Alternate gene names: 52140174

Gene position: 5197220-5198476 (Clockwise)

Preceding gene: 52140176

Following gene: 52140175

Centisome position: 98.04

GC content: 35.0

Gene sequence:

>1257_bases
ATGAAGAAAATCTTGTCTATTTTACTAGCACTTTTATTGTCAATTTCTTCCTTAGGAGTAACAACATCCCATGCGGAAGA
GAAAATACACATTGAGGCGGCGGCTGCACTCCTATTTGATGCAGATACAGGAAAAATACTGCATGAACAAAATCCTGATG
AATTACTAGCTATCGCTAGTATGTCAAAGTTAATTGTTGTTTATGCTGTATTAGAAGCAATTAAAGAAGGAAAAATCACA
TGGGATACAAAGGTAAACATCTCTGATTATGCTTATGAAGTTTCACGTAATAACGAATTCTCTAACGTACCGTTCGAAAA
AGGACGTCAATATACTGTAAGAGAACTATATCATTCTATCGTTATATTCTCTGCAAACGGATCTAGTATTGCCTTAGCAG
AACTTCTTGCAGGAAGTGAGAAAAACTTCTTAAATCTTGCAAATGAACATGCAAAAAAATTAGGGTTAAAGAAATATAAA
TTTGTAAACGCTACTGGTTTAAATAACGCTGACTTAAAAGGAAAACATCCTGAGGGTACTGATCCAAATGCAGAAAACTC
TATGTCAGCTCGCGATATGGGTATACTTTCAAAAGCAATGATTACAAAGTATCCAGAAATGCTAGAGGATACAAAACAAA
GATTTAGAAACTTCCCAGATAATCATCCGAAACCAATTCGTATGGAAAACTGGAACTGGATGTTACCAGGAGCTGCTTTC
GCTTATGAAGGTACTGATGGTTTAAAAACCGGAAGTTCTGATACAGCTGGATATGGATTTACTATTACTGCTAAACGTGG
TGATGTACGTCTTATTTCGGTTATTATTAAAACAAAATCAATGGACGAGCGCTTCACAGAATCTCGTGAATTAATCGAAT
ACGGGTTTAATAACTTCGAAAAACAAAAATTAAAGATTAATAAAAACAATACAATCTCTGTAGTACAAGGGAAAGAAGAC
CAAGTCACTGTTACACCTGAAAAAGAAATAACAGTAATTGCGAAGAAAGGTAGTAAAGATCCTTATAAAATTGGGACTGA
GGTAGATAAATCCCTCGCTGAAGATGGACATTTAGTTGCCCCTGTTAAGAAAGGTGCCAAAGTAGGATCGATTACTTTAG
AATCAACTGATAAATATGGTTTCTTAGACGGTAATAAAAGTATGAAAGTTACCGCAAAAACGACAGAAGAAGTTGAAAAA
GCAAACTGGTTTGTGTTATCAATGCGCTCTATTGGAGATTTCTTCTCAAACTTATGA

Upstream 100 bases:

>100_bases
CTTTTCAAATTTCCTATAAAATTGTAACCTTTTCATATCCAAATTTATGCTAAAATGGTACGAGCTACATGCTAATAACG
ATGCGAAGGTGATAATCTAT

Downstream 100 bases:

>100_bases
TCTAAAGTATTTTAATAATGAAAAGCTAGCTCACGCTAGCTTTTTTTATATTCTTCTCTCAATGTAAAACTTCGTTTGGT
TGTCATATCTGAGTCCTTTT

Product: D-alanyl-D-alanine carboxypeptidase (serine-type D-Ala-D-Ala carboxypeptidase)

Products: NA

Alternate protein names: CPase; DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5; PBP-5 [H]

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MKKILSILLALLLSISSLGVTTSHAEEKIHIEAAAALLFDADTGKILHEQNPDELLAIASMSKLIVVYAVLEAIKEGKIT
WDTKVNISDYAYEVSRNNEFSNVPFEKGRQYTVRELYHSIVIFSANGSSIALAELLAGSEKNFLNLANEHAKKLGLKKYK
FVNATGLNNADLKGKHPEGTDPNAENSMSARDMGILSKAMITKYPEMLEDTKQRFRNFPDNHPKPIRMENWNWMLPGAAF
AYEGTDGLKTGSSDTAGYGFTITAKRGDVRLISVIIKTKSMDERFTESRELIEYGFNNFEKQKLKINKNNTISVVQGKED
QVTVTPEKEITVIAKKGSKDPYKIGTEVDKSLAEDGHLVAPVKKGAKVGSITLESTDKYGFLDGNKSMKVTAKTTEEVEK
ANWFVLSMRSIGDFFSNL

Sequences:

>Translated_418_residues
MKKILSILLALLLSISSLGVTTSHAEEKIHIEAAAALLFDADTGKILHEQNPDELLAIASMSKLIVVYAVLEAIKEGKIT
WDTKVNISDYAYEVSRNNEFSNVPFEKGRQYTVRELYHSIVIFSANGSSIALAELLAGSEKNFLNLANEHAKKLGLKKYK
FVNATGLNNADLKGKHPEGTDPNAENSMSARDMGILSKAMITKYPEMLEDTKQRFRNFPDNHPKPIRMENWNWMLPGAAF
AYEGTDGLKTGSSDTAGYGFTITAKRGDVRLISVIIKTKSMDERFTESRELIEYGFNNFEKQKLKINKNNTISVVQGKED
QVTVTPEKEITVIAKKGSKDPYKIGTEVDKSLAEDGHLVAPVKKGAKVGSITLESTDKYGFLDGNKSMKVTAKTTEEVEK
ANWFVLSMRSIGDFFSNL
>Mature_418_residues
MKKILSILLALLLSISSLGVTTSHAEEKIHIEAAAALLFDADTGKILHEQNPDELLAIASMSKLIVVYAVLEAIKEGKIT
WDTKVNISDYAYEVSRNNEFSNVPFEKGRQYTVRELYHSIVIFSANGSSIALAELLAGSEKNFLNLANEHAKKLGLKKYK
FVNATGLNNADLKGKHPEGTDPNAENSMSARDMGILSKAMITKYPEMLEDTKQRFRNFPDNHPKPIRMENWNWMLPGAAF
AYEGTDGLKTGSSDTAGYGFTITAKRGDVRLISVIIKTKSMDERFTESRELIEYGFNNFEKQKLKINKNNTISVVQGKED
QVTVTPEKEITVIAKKGSKDPYKIGTEVDKSLAEDGHLVAPVKKGAKVGSITLESTDKYGFLDGNKSMKVTAKTTEEVEK
ANWFVLSMRSIGDFFSNL

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted, cell wall [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=409, Percent_Identity=30.3178484107579, Blast_Score=145, Evalue=6e-36,
Organism=Escherichia coli, GI1786851, Length=410, Percent_Identity=28.5365853658537, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI87082022, Length=429, Percent_Identity=27.039627039627, Blast_Score=121, Evalue=7e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 46363; Mature: 46363

Theoretical pI: Translated: 8.56; Mature: 8.56

Prosite motif: PS00018 EF_HAND_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKILSILLALLLSISSLGVTTSHAEEKIHIEAAAALLFDADTGKILHEQNPDELLAIAS
CHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEEEEECCCCCEECCCCHHHHHHHHH
MSKLIVVYAVLEAIKEGKITWDTKVNISDYAYEVSRNNEFSNVPFEKGRQYTVRELYHSI
HHHHHHHHHHHHHHHCCCEEEEEEECCHHHEEEECCCCCCCCCCHHCCCCHHHHHHHCEE
VIFSANGSSIALAELLAGSEKNFLNLANEHAKKLGLKKYKFVNATGLNNADLKGKHPEGT
EEEECCCCCCHHHHHHCCCCCHHHHHHHHHHHHCCCHHEEEEECCCCCCCCCCCCCCCCC
DPNAENSMSARDMGILSKAMITKYPEMLEDTKQRFRNFPDNHPKPIRMENWNWMLPGAAF
CCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCEEECCCCEECCCCEE
AYEGTDGLKTGSSDTAGYGFTITAKRGDVRLISVIIKTKSMDERFTESRELIEYGFNNFE
EECCCCCCCCCCCCCCCCCEEEEECCCCEEEEEEEEHHCCHHHHHHHHHHHHHHCCCCCC
KQKLKINKNNTISVVQGKEDQVTVTPEKEITVIAKKGSKDPYKIGTEVDKSLAEDGHLVA
CEEEEECCCCEEEEEECCCCCEEECCCCCEEEEEECCCCCCCCCCCHHHHHHHCCCCEEE
PVKKGAKVGSITLESTDKYGFLDGNKSMKVTAKTTEEVEKANWFVLSMRSIGDFFSNL
EHHCCCEEEEEEEECCCCEEEECCCCCEEEEECCHHHHHHCCEEEEEHHHHHHHHHCC
>Mature Secondary Structure
MKKILSILLALLLSISSLGVTTSHAEEKIHIEAAAALLFDADTGKILHEQNPDELLAIAS
CHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEEEEECCCCCEECCCCHHHHHHHHH
MSKLIVVYAVLEAIKEGKITWDTKVNISDYAYEVSRNNEFSNVPFEKGRQYTVRELYHSI
HHHHHHHHHHHHHHHCCCEEEEEEECCHHHEEEECCCCCCCCCCHHCCCCHHHHHHHCEE
VIFSANGSSIALAELLAGSEKNFLNLANEHAKKLGLKKYKFVNATGLNNADLKGKHPEGT
EEEECCCCCCHHHHHHCCCCCHHHHHHHHHHHHCCCHHEEEEECCCCCCCCCCCCCCCCC
DPNAENSMSARDMGILSKAMITKYPEMLEDTKQRFRNFPDNHPKPIRMENWNWMLPGAAF
CCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCEEECCCCEECCCCEE
AYEGTDGLKTGSSDTAGYGFTITAKRGDVRLISVIIKTKSMDERFTESRELIEYGFNNFE
EECCCCCCCCCCCCCCCCCEEEEECCCCEEEEEEEEHHCCHHHHHHHHHHHHHHCCCCCC
KQKLKINKNNTISVVQGKEDQVTVTPEKEITVIAKKGSKDPYKIGTEVDKSLAEDGHLVA
CEEEEECCCCEEEEEECCCCCEEECCCCCEEEEEECCCCCCCCCCCHHHHHHHCCCCEEE
PVKKGAKVGSITLESTDKYGFLDGNKSMKVTAKTTEEVEKANWFVLSMRSIGDFFSNL
EHHCCCEEEEEEEECCCCEEEECCCCCEEEEECCHHHHHHCCEEEEEHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8370539; 6780559; 111240 [H]