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The map label for this gene is mdeA [H]

Identifier: 51893920

GI number: 51893920

Start: 2999868

End: 3001148

Strand: Reverse

Name: mdeA [H]

Synonym: STH2782

Alternate gene names: 51893920

Gene position: 3001148-2999868 (Counterclockwise)

Preceding gene: 51893921

Following gene: 51893919

Centisome position: 84.16

GC content: 68.7

Gene sequence:

>1281_bases
TTGGCAGAACGGAAGTACCGGCTCGAGACACTGGCCATTCACGCGGGCCACGAGATCGATCCCACGGGGGCGCGGGCGGT
CCCGCTGTACATGACCACCTCCTACGTGTTCCGGGACACGGCGCACGCGGCCCGGCTCTTCAGCCTGCAGGAGCCGGGCA
ACATCTACACCCGCATCATGAACCCGACCACGGACGTGTTCGAGAAGCGGATGGCCGCCCTGGAGGGGGGCGTGGGGGCC
CTCGCCGTGGCCTCGGGGCAGAATGCGGAACTGTACGCCATCCTGAACCTGGCCCGGGCGGGCGACGAGATCGTCGCCGC
CACGAGCCTGTACGGCGGCACGTATGCCCTGTTCGCCCACAACCTGCCCCGCCTGGGCATCACGGTGAAGTTCGTCGAGC
CTTCCGACCCCGAAAACTTCCGTGCGGCCATCACGGACCGCACCCGGGCGCTTTACATTGAGACCATCGGCAATCCCCGG
TGCGACGTGCCGGACATCGCGGCGATTGCCGCCGTCGCCCACGAGCACGGGCTGCCGCTCATCGTCGACAACACCTTCGC
CTCGCCGTACCTCTGCCGTCCCATCGAGCACGGCGCCGACATCGTGATTCACTCGGCCACCAAGTTCATCGGCGGCCACG
GCACCGCGATCGGCGGCGTGATCGTCGACGGCGGCCGGTTCGACTGGGGCGCCGGCAGGCATCCGCTCTACACGGAGCCC
GATCCGGCCTATCACGGCCTGCGCTACGCCGACCTGGGCGAGCAGGCGTACATCCTGCGGGCCCGGGTGAGCCTTCTGCG
CGACATGGGCGGCGCCCTCTCGCCTTTCAACGCGTTCCTGTTCCTGCAGGGGCTGGAGACGCTGCACCTGCGGATGGAGC
GGCATTCGGCCAACGCCCTGGCCGTCGCCCGGTTCCTGGAGGCGCACCCCAAGGTGGAGTGGGTCACCTACCCCGGGTTG
GAGTCGAGCCCGTATTACGGGAACGCCAAGCGGTACCTGCCCCGGGGGCAGGGGGCCATCCTCGCCTTCGGCATCCGCGG
CGGGCTGGAGGCAGGGCGCCGCTTCATCGAGTCGCTCCGGCTGTTCTCCCACCTGGCCAACGTGGGCGATTCCAAGTCGC
TGGCGATTCACCCTGCTTCAACCACCCACGAGCAGCTGTCCGACGAGGAGCGTCGGGCAGCAGGTGTCACGCCCGAGATG
GTGCGGCTCTCGGTGGGCACCGAGCACATCGACGATATCATCGAGGACGTGGCCCAGGCCCTGGAGCGGGCGACGAACTG
A

Upstream 100 bases:

>100_bases
CCCCAACTGTATGCATATCGTAGTACAGTTGCTGGATCCGCCGGCAGGGCCGTGTTAGGATACAGTCACCTTCGAGTGCG
AAGAGTGGGGAGTGGTGGCG

Downstream 100 bases:

>100_bases
CGCGGCGGCAGCAGCGGGAGGGGCGTGCCGGTTCGACAGACGGTGCGTCCCTCCCGGTTATGTCGTTGAACGCGAAATAA
TCTGTCAACAGTTGACTCGC

Product: O-acetylhomoserine aminocarboxypropyltransferase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MAERKYRLETLAIHAGHEIDPTGARAVPLYMTTSYVFRDTAHAARLFSLQEPGNIYTRIMNPTTDVFEKRMAALEGGVGA
LAVASGQNAELYAILNLARAGDEIVAATSLYGGTYALFAHNLPRLGITVKFVEPSDPENFRAAITDRTRALYIETIGNPR
CDVPDIAAIAAVAHEHGLPLIVDNTFASPYLCRPIEHGADIVIHSATKFIGGHGTAIGGVIVDGGRFDWGAGRHPLYTEP
DPAYHGLRYADLGEQAYILRARVSLLRDMGGALSPFNAFLFLQGLETLHLRMERHSANALAVARFLEAHPKVEWVTYPGL
ESSPYYGNAKRYLPRGQGAILAFGIRGGLEAGRRFIESLRLFSHLANVGDSKSLAIHPASTTHEQLSDEERRAAGVTPEM
VRLSVGTEHIDDIIEDVAQALERATN

Sequences:

>Translated_426_residues
MAERKYRLETLAIHAGHEIDPTGARAVPLYMTTSYVFRDTAHAARLFSLQEPGNIYTRIMNPTTDVFEKRMAALEGGVGA
LAVASGQNAELYAILNLARAGDEIVAATSLYGGTYALFAHNLPRLGITVKFVEPSDPENFRAAITDRTRALYIETIGNPR
CDVPDIAAIAAVAHEHGLPLIVDNTFASPYLCRPIEHGADIVIHSATKFIGGHGTAIGGVIVDGGRFDWGAGRHPLYTEP
DPAYHGLRYADLGEQAYILRARVSLLRDMGGALSPFNAFLFLQGLETLHLRMERHSANALAVARFLEAHPKVEWVTYPGL
ESSPYYGNAKRYLPRGQGAILAFGIRGGLEAGRRFIESLRLFSHLANVGDSKSLAIHPASTTHEQLSDEERRAAGVTPEM
VRLSVGTEHIDDIIEDVAQALERATN
>Mature_425_residues
AERKYRLETLAIHAGHEIDPTGARAVPLYMTTSYVFRDTAHAARLFSLQEPGNIYTRIMNPTTDVFEKRMAALEGGVGAL
AVASGQNAELYAILNLARAGDEIVAATSLYGGTYALFAHNLPRLGITVKFVEPSDPENFRAAITDRTRALYIETIGNPRC
DVPDIAAIAAVAHEHGLPLIVDNTFASPYLCRPIEHGADIVIHSATKFIGGHGTAIGGVIVDGGRFDWGAGRHPLYTEPD
PAYHGLRYADLGEQAYILRARVSLLRDMGGALSPFNAFLFLQGLETLHLRMERHSANALAVARFLEAHPKVEWVTYPGLE
SSPYYGNAKRYLPRGQGAILAFGIRGGLEAGRRFIESLRLFSHLANVGDSKSLAIHPASTTHEQLSDEERRAAGVTPEMV
RLSVGTEHIDDIIEDVAQALERATN

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=416, Percent_Identity=36.2980769230769, Blast_Score=228, Evalue=8e-60,
Organism=Homo sapiens, GI299473759, Length=416, Percent_Identity=32.9326923076923, Blast_Score=187, Evalue=2e-47,
Organism=Homo sapiens, GI25453487, Length=415, Percent_Identity=31.3253012048193, Blast_Score=170, Evalue=2e-42,
Organism=Escherichia coli, GI1790375, Length=419, Percent_Identity=31.980906921241, Blast_Score=197, Evalue=8e-52,
Organism=Escherichia coli, GI1789383, Length=439, Percent_Identity=25.2847380410023, Blast_Score=117, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17538055, Length=422, Percent_Identity=34.5971563981043, Blast_Score=216, Evalue=2e-56,
Organism=Caenorhabditis elegans, GI71980708, Length=408, Percent_Identity=33.3333333333333, Blast_Score=211, Evalue=5e-55,
Organism=Caenorhabditis elegans, GI17560056, Length=420, Percent_Identity=34.5238095238095, Blast_Score=210, Evalue=1e-54,
Organism=Saccharomyces cerevisiae, GI6323333, Length=432, Percent_Identity=50.6944444444444, Blast_Score=397, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6319307, Length=428, Percent_Identity=34.8130841121495, Blast_Score=214, Evalue=2e-56,
Organism=Saccharomyces cerevisiae, GI6321254, Length=443, Percent_Identity=26.86230248307, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6321170, Length=298, Percent_Identity=27.8523489932886, Blast_Score=119, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6321904, Length=277, Percent_Identity=28.158844765343, Blast_Score=94, Evalue=5e-20,
Organism=Drosophila melanogaster, GI19922554, Length=429, Percent_Identity=33.5664335664336, Blast_Score=218, Evalue=5e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 46387; Mature: 46256

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAERKYRLETLAIHAGHEIDPTGARAVPLYMTTSYVFRDTAHAARLFSLQEPGNIYTRIM
CCCCCEEEEEEEEECCCCCCCCCCCEEEEEEEEHHHHHHHHHHHHHEECCCCCCEEEEEE
NPTTDVFEKRMAALEGGVGALAVASGQNAELYAILNLARAGDEIVAATSLYGGTYALFAH
CCCHHHHHHHHHHHCCCCCEEEEECCCCCEEEEEEEHHHCCCCEEEEEECCCCEEEEECC
NLPRLGITVKFVEPSDPENFRAAITDRTRALYIETIGNPRCDVPDIAAIAAVAHEHGLPL
CCCCCCEEEEEECCCCCCCHHEEEECCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCEE
IVDNTFASPYLCRPIEHGADIVIHSATKFIGGHGTAIGGVIVDGGRFDWGAGRHPLYTEP
EEECCCCCCEEECCCCCCCCEEEEECHHHHCCCCCEECEEEEECCEECCCCCCCCCCCCC
DPAYHGLRYADLGEQAYILRARVSLLRDMGGALSPFNAFLFLQGLETLHLRMERHSANAL
CCHHCCCEEECCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHH
AVARFLEAHPKVEWVTYPGLESSPYYGNAKRYLPRGQGAILAFGIRGGLEAGRRFIESLR
HHHHHHHHCCCEEEEECCCCCCCCCCCCCHHCCCCCCCCEEEEEECCCHHHHHHHHHHHH
LFSHLANVGDSKSLAIHPASTTHEQLSDEERRAAGVTPEMVRLSVGTEHIDDIIEDVAQA
HHHHHHCCCCCCEEEEECCCCCHHHHCCHHHHHCCCCHHHEEEECCHHHHHHHHHHHHHH
LERATN
HHHHCC
>Mature Secondary Structure 
AERKYRLETLAIHAGHEIDPTGARAVPLYMTTSYVFRDTAHAARLFSLQEPGNIYTRIM
CCCCEEEEEEEEECCCCCCCCCCCEEEEEEEEHHHHHHHHHHHHHEECCCCCCEEEEEE
NPTTDVFEKRMAALEGGVGALAVASGQNAELYAILNLARAGDEIVAATSLYGGTYALFAH
CCCHHHHHHHHHHHCCCCCEEEEECCCCCEEEEEEEHHHCCCCEEEEEECCCCEEEEECC
NLPRLGITVKFVEPSDPENFRAAITDRTRALYIETIGNPRCDVPDIAAIAAVAHEHGLPL
CCCCCCEEEEEECCCCCCCHHEEEECCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCEE
IVDNTFASPYLCRPIEHGADIVIHSATKFIGGHGTAIGGVIVDGGRFDWGAGRHPLYTEP
EEECCCCCCEEECCCCCCCCEEEEECHHHHCCCCCEECEEEEECCEECCCCCCCCCCCCC
DPAYHGLRYADLGEQAYILRARVSLLRDMGGALSPFNAFLFLQGLETLHLRMERHSANAL
CCHHCCCEEECCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHH
AVARFLEAHPKVEWVTYPGLESSPYYGNAKRYLPRGQGAILAFGIRGGLEAGRRFIESLR
HHHHHHHHCCCEEEEECCCCCCCCCCCCCHHCCCCCCCCEEEEEECCCHHHHHHHHHHHH
LFSHLANVGDSKSLAIHPASTTHEQLSDEERRAAGVTPEMVRLSVGTEHIDDIIEDVAQA
HHHHHHCCCCCCEEEEECCCCCHHHHCCHHHHHCCCCHHHEEEECCHHHHHHHHHHHHHH
LERATN
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]