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The map label for this gene is pilJ [H]

Identifier: 51893903

GI number: 51893903

Start: 2983340

End: 2984596

Strand: Reverse

Name: pilJ [H]

Synonym: STH2765

Alternate gene names: 51893903

Gene position: 2984596-2983340 (Counterclockwise)

Preceding gene: 51893905

Following gene: 51893901

Centisome position: 83.69

GC content: 72.0

Gene sequence:

>1257_bases
GTGAAACAATCCCCGTGGTCGCGAGGAGGGATCCCCGTGTTCTGGCGCGCCTCGGCCAGCAGTCTGGTCACCGGGGGCAT
CGTGGCGGTTGCCTTTCTTATTCCTTCACCGATGGCTCGGTACTCCCTGCTTGCGGCCGCCCTGCTGGTGTCCGTGCTGG
GCGGGCTGTGGGTGGGGCGTGCGGCGCAGGCCGGAGCCGCCGCGCCTCCGGATATGCAGCTCTCCCCGCCGGAGACGGGG
AGCGTCGCCGTCTCCGTGTCCGCCGCCGCGGAGGCCGCTGCGGGCCTGCAGCCCCACGCGGACACGCACCGCACCGGGAG
CGCCGACCGGGCCGACCTGGCCCGGGCCTGTGACGGGCTCATCATCGGTGCACAACGCACGCAGGAGGCGGTACAGGAGG
TCTTCCGCCTCACGGGCCGGGTCAACGACGTGCTCAACCAGCTGCACGGCGCTTCCCGGGATCAGCTGGACGACCTCGAC
CGGACCCGCGGGCTGGCCCATCAGGTGGTGGCGGTGTTTGAGGACATGATCGCGGCCGCCCGGGCGGCCCGGGAGGAGGC
GGCGCGCCACCGCCAGGCGGCGGAGGCCGTGCAGCAGGCCTTGGGCCAGATCGGCGCGGGTATGGAGGGGATTCGCGCCG
CCACCGACGCATCGGCACGGGCTCTGCGGGATCTGGACGCGCAGTCCGGCGAGATCGGCGCGATCGTGAAGCTGATCCGG
GACGTGGCCGACCAGACCAACCTGCTGTCGCTGAACGCAGCCATCGAGGCGGCGCGGGCCGGCGAGGCCGGCCGCGGCTT
CGCCGTGGTGGCCTCCGAGGTGCGCGCCCTGGCCGATCGTTCCCGCAACGCCACCCGGCAGATCCAGGAGCTGGTGGCCA
AGATCCAGGCCGGCACTGCCGCCGCGATGACGGCCATGCAGGAGTCGCAGGAGGAAGTCAACCGGGGCGCCGCGACCGTG
GAGTCGACCCGCGCGTCCATCGTTCAGGTGCTGCAGTCGTTCGAGGGGCTCACTATGACGGTCGAGGGCTTCGGCGAACG
GGCTGAGGCCACGGCCGGGGAGATGGCGGAGCTCGTGAAGGCGGTGGAGGAGGCGACACGGCTCGCCAATCAGAACACCA
CCATGGCGAACGAGCTGGCCGAGGCCGACTGGTTCTCGCGGGCGATCCGGGAGGCTGAGCAGCGGGCCGGCGAACTGGTC
GCCGAGGCCAGGCGGCTCGAGGTCTACACGCGCTTGCCTTCCGGTGGCTCCTGGTAG

Upstream 100 bases:

>100_bases
CCCAGCGTTTACTGATCCGTCATCATTTTAACACATTAGATGAGTCGAGACGGGGGTGCAGGAAGATTCCTGCAGGGTGC
TGAAACAATAAACAATCATC

Downstream 100 bases:

>100_bases
AGGTGGGGGAACTGCTTCCGCCAGCCGAAGCGCCAGAGGAGCCACCAGGGCAGCCAGCCCAGCCCGGCGGTCCCGGCTGC
GGCAGCGCCCGCGCCCCCTG

Product: methyl-accepting chemotaxis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MKQSPWSRGGIPVFWRASASSLVTGGIVAVAFLIPSPMARYSLLAAALLVSVLGGLWVGRAAQAGAAAPPDMQLSPPETG
SVAVSVSAAAEAAAGLQPHADTHRTGSADRADLARACDGLIIGAQRTQEAVQEVFRLTGRVNDVLNQLHGASRDQLDDLD
RTRGLAHQVVAVFEDMIAAARAAREEAARHRQAAEAVQQALGQIGAGMEGIRAATDASARALRDLDAQSGEIGAIVKLIR
DVADQTNLLSLNAAIEAARAGEAGRGFAVVASEVRALADRSRNATRQIQELVAKIQAGTAAAMTAMQESQEEVNRGAATV
ESTRASIVQVLQSFEGLTMTVEGFGERAEATAGEMAELVKAVEEATRLANQNTTMANELAEADWFSRAIREAEQRAGELV
AEARRLEVYTRLPSGGSW

Sequences:

>Translated_418_residues
MKQSPWSRGGIPVFWRASASSLVTGGIVAVAFLIPSPMARYSLLAAALLVSVLGGLWVGRAAQAGAAAPPDMQLSPPETG
SVAVSVSAAAEAAAGLQPHADTHRTGSADRADLARACDGLIIGAQRTQEAVQEVFRLTGRVNDVLNQLHGASRDQLDDLD
RTRGLAHQVVAVFEDMIAAARAAREEAARHRQAAEAVQQALGQIGAGMEGIRAATDASARALRDLDAQSGEIGAIVKLIR
DVADQTNLLSLNAAIEAARAGEAGRGFAVVASEVRALADRSRNATRQIQELVAKIQAGTAAAMTAMQESQEEVNRGAATV
ESTRASIVQVLQSFEGLTMTVEGFGERAEATAGEMAELVKAVEEATRLANQNTTMANELAEADWFSRAIREAEQRAGELV
AEARRLEVYTRLPSGGSW
>Mature_418_residues
MKQSPWSRGGIPVFWRASASSLVTGGIVAVAFLIPSPMARYSLLAAALLVSVLGGLWVGRAAQAGAAAPPDMQLSPPETG
SVAVSVSAAAEAAAGLQPHADTHRTGSADRADLARACDGLIIGAQRTQEAVQEVFRLTGRVNDVLNQLHGASRDQLDDLD
RTRGLAHQVVAVFEDMIAAARAAREEAARHRQAAEAVQQALGQIGAGMEGIRAATDASARALRDLDAQSGEIGAIVKLIR
DVADQTNLLSLNAAIEAARAGEAGRGFAVVASEVRALADRSRNATRQIQELVAKIQAGTAAAMTAMQESQEEVNRGAATV
ESTRASIVQVLQSFEGLTMTVEGFGERAEATAGEMAELVKAVEEATRLANQNTTMANELAEADWFSRAIREAEQRAGELV
AEARRLEVYTRLPSGGSW

Specific function: May be a part of a signal-transduction system that regulates twitching motility by controlling pilus function (extension and retraction) [H]

COG id: COG0840

COG function: function code NT; Methyl-accepting chemotaxis protein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 methyl-accepting transducer domain [H]

Homologues:

Organism=Escherichia coli, GI1789453, Length=162, Percent_Identity=35.8024691358025, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1788194, Length=223, Percent_Identity=32.7354260089686, Blast_Score=85, Evalue=8e-18,
Organism=Escherichia coli, GI2367378, Length=160, Percent_Identity=36.875, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1788195, Length=195, Percent_Identity=32.3076923076923, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1787690, Length=154, Percent_Identity=31.8181818181818, Blast_Score=77, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004090
- InterPro:   IPR004089
- InterPro:   IPR003660 [H]

Pfam domain/function: PF00015 MCPsignal [H]

EC number: NA

Molecular weight: Translated: 43862; Mature: 43862

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS50111 CHEMOTAXIS_TRANSDUC_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQSPWSRGGIPVFWRASASSLVTGGIVAVAFLIPSPMARYSLLAAALLVSVLGGLWVGR
CCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
AAQAGAAAPPDMQLSPPETGSVAVSVSAAAEAAAGLQPHADTHRTGSADRADLARACDGL
HHCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCE
IIGAQRTQEAVQEVFRLTGRVNDVLNQLHGASRDQLDDLDRTRGLAHQVVAVFEDMIAAA
EECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RAAREEAARHRQAAEAVQQALGQIGAGMEGIRAATDASARALRDLDAQSGEIGAIVKLIR
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
DVADQTNLLSLNAAIEAARAGEAGRGFAVVASEVRALADRSRNATRQIQELVAKIQAGTA
HHHCCCCEEEHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHH
AAMTAMQESQEEVNRGAATVESTRASIVQVLQSFEGLTMTVEGFGERAEATAGEMAELVK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHCCCCHHHHHHHHHHHHHH
AVEEATRLANQNTTMANELAEADWFSRAIREAEQRAGELVAEARRLEVYTRLPSGGSW
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKQSPWSRGGIPVFWRASASSLVTGGIVAVAFLIPSPMARYSLLAAALLVSVLGGLWVGR
CCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
AAQAGAAAPPDMQLSPPETGSVAVSVSAAAEAAAGLQPHADTHRTGSADRADLARACDGL
HHCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCE
IIGAQRTQEAVQEVFRLTGRVNDVLNQLHGASRDQLDDLDRTRGLAHQVVAVFEDMIAAA
EECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RAAREEAARHRQAAEAVQQALGQIGAGMEGIRAATDASARALRDLDAQSGEIGAIVKLIR
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
DVADQTNLLSLNAAIEAARAGEAGRGFAVVASEVRALADRSRNATRQIQELVAKIQAGTA
HHHCCCCEEEHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHH
AAMTAMQESQEEVNRGAATVESTRASIVQVLQSFEGLTMTVEGFGERAEATAGEMAELVK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHCCCCHHHHHHHHHHHHHH
AVEEATRLANQNTTMANELAEADWFSRAIREAEQRAGELVAEARRLEVYTRLPSGGSW
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7908398; 10984043 [H]