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The map label for this gene is tgt [H]

Identifier: 51599061

GI number: 51599061

Start: 856759

End: 857886

Strand: Direct

Name: tgt [H]

Synonym: BG0835

Alternate gene names: 51599061

Gene position: 856759-857886 (Clockwise)

Preceding gene: 51599060

Following gene: 51599062

Centisome position: 94.75

GC content: 31.29

Gene sequence:

>1128_bases
ATGTTTAGCGTAATTAAGAATGACAAACATTCTAATGCAAGGGTTGGATTTCTAAATCTTCCTCATGGCAGAGTAGATAC
TCCTTGCTTTATGCCAGTTGGTACTTTAGGAGCAATGAAAGGCTTAAAGCATTCTGTTCTTGAGAAGTTAAAATGTAATT
TAATGCTTGCAAATACGTATCATTTATATTTAAGACCGGGCATTAAAACTATTGAAAAATATGGTGGTCTTCATAATTTT
ACAACTTGGAATAAAAATTTTTTAACCGATTCTGGTGGATTTCAGGTGTTTTCTCTTTCTAGTCTAAGAAAAATTGATCT
AAAAGGTGTGCGTTTTAAATCTCATCTAGATGGATCTTATCATTATTTTACTCCTGAGGGAGTATTTGCTATGCAAGAAA
TTTTTGGCAGCGATATTATTATGCCACTTGATATTTGTAGTTCTTATGGTATTGATTATAATGAAGCCAATTTGTATACA
AATATTACAACCAATTGGGCTCGTAGCACATTTAAGGCTTATAAAAACAGAAAAGAGGGATACAACGGGCTTTTATTTTT
AATAACTCAGGGAAACTTTTTTAAAGATTTAAGAAAAAGAAGCATCAATGATATATTAGAATTAGACAGTCCAGGTATTG
CTATTGGGGGCATTTCTGTTGGAGAACCAAGAGAAAAATATTTAGAAATTCTTGAGTATAGTTCTTTGTTAATGCCAAAA
GAAAAACCAAGATATGTAATGGGTATTGGTACTCCCCATTACATACTTGATGCCATATACCATGGCATTGATATTTTTGA
TTGTGTTAATCCTGCAAGAATTGCTAGGCACGGGTCTCTTTTAACAGATAATGGAATAATGCGCATTAGTAGAAAAGAGT
ATAAAGATGATACTTCCCAGGTAGAAAAGAATTGTGGCTGTACTTTATGTACAAGATATTCAAGAGGATATTTAAGGCAT
TTGATAAAATCTAAAGAGCTTTTTGGAATAATTTTGGCAAGTGAGCATAATATTTACTATATGTTCCGATTGATTTCAAA
GATCAGAACCGCAATTCTAAATGATAATTTTTTAAACTTTAGAACTTCATATTTAAAAAAGTATGAAGAGGAAAATTTCG
ATGAATAA

Upstream 100 bases:

>100_bases
TGGCAAGCTCTTTTACAACTATATTTTTTTTGATTATCAATTTTTTTGTTTATAGGATTGTTAGGAAATAATATATATAA
TAATTGTTTAGGATATTTAT

Downstream 100 bases:

>100_bases
ATATGTTATTTCTACAGTTTTGGTCATGATTTCTACCTTTTTTTCAAGAATAATGGGGTTTATAAAGGTAAAGATTTTCT
CTTATTATTTTGGAGCAAAT

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MFSVIKNDKHSNARVGFLNLPHGRVDTPCFMPVGTLGAMKGLKHSVLEKLKCNLMLANTYHLYLRPGIKTIEKYGGLHNF
TTWNKNFLTDSGGFQVFSLSSLRKIDLKGVRFKSHLDGSYHYFTPEGVFAMQEIFGSDIIMPLDICSSYGIDYNEANLYT
NITTNWARSTFKAYKNRKEGYNGLLFLITQGNFFKDLRKRSINDILELDSPGIAIGGISVGEPREKYLEILEYSSLLMPK
EKPRYVMGIGTPHYILDAIYHGIDIFDCVNPARIARHGSLLTDNGIMRISRKEYKDDTSQVEKNCGCTLCTRYSRGYLRH
LIKSKELFGIILASEHNIYYMFRLISKIRTAILNDNFLNFRTSYLKKYEEENFDE

Sequences:

>Translated_375_residues
MFSVIKNDKHSNARVGFLNLPHGRVDTPCFMPVGTLGAMKGLKHSVLEKLKCNLMLANTYHLYLRPGIKTIEKYGGLHNF
TTWNKNFLTDSGGFQVFSLSSLRKIDLKGVRFKSHLDGSYHYFTPEGVFAMQEIFGSDIIMPLDICSSYGIDYNEANLYT
NITTNWARSTFKAYKNRKEGYNGLLFLITQGNFFKDLRKRSINDILELDSPGIAIGGISVGEPREKYLEILEYSSLLMPK
EKPRYVMGIGTPHYILDAIYHGIDIFDCVNPARIARHGSLLTDNGIMRISRKEYKDDTSQVEKNCGCTLCTRYSRGYLRH
LIKSKELFGIILASEHNIYYMFRLISKIRTAILNDNFLNFRTSYLKKYEEENFDE
>Mature_375_residues
MFSVIKNDKHSNARVGFLNLPHGRVDTPCFMPVGTLGAMKGLKHSVLEKLKCNLMLANTYHLYLRPGIKTIEKYGGLHNF
TTWNKNFLTDSGGFQVFSLSSLRKIDLKGVRFKSHLDGSYHYFTPEGVFAMQEIFGSDIIMPLDICSSYGIDYNEANLYT
NITTNWARSTFKAYKNRKEGYNGLLFLITQGNFFKDLRKRSINDILELDSPGIAIGGISVGEPREKYLEILEYSSLLMPK
EKPRYVMGIGTPHYILDAIYHGIDIFDCVNPARIARHGSLLTDNGIMRISRKEYKDDTSQVEKNCGCTLCTRYSRGYLRH
LIKSKELFGIILASEHNIYYMFRLISKIRTAILNDNFLNFRTSYLKKYEEENFDE

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=367, Percent_Identity=38.9645776566758, Blast_Score=273, Evalue=2e-73,
Organism=Escherichia coli, GI1786607, Length=359, Percent_Identity=38.9972144846797, Blast_Score=288, Evalue=4e-79,
Organism=Caenorhabditis elegans, GI17544680, Length=357, Percent_Identity=36.9747899159664, Blast_Score=244, Evalue=6e-65,
Organism=Caenorhabditis elegans, GI25146502, Length=197, Percent_Identity=26.9035532994924, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19920510, Length=367, Percent_Identity=38.4196185286104, Blast_Score=276, Evalue=2e-74,
Organism=Drosophila melanogaster, GI21355311, Length=141, Percent_Identity=31.9148936170213, Blast_Score=78, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 43064; Mature: 43064

Theoretical pI: Translated: 9.22; Mature: 9.22

Prosite motif: PS01109 RIBOSOMAL_L10

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSVIKNDKHSNARVGFLNLPHGRVDTPCFMPVGTLGAMKGLKHSVLEKLKCNLMLANTY
CCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEE
HLYLRPGIKTIEKYGGLHNFTTWNKNFLTDSGGFQVFSLSSLRKIDLKGVRFKSHLDGSY
EEEECCCHHHHHHHCCCCCCCCCCCCEEECCCCEEEEEECCCCEECCCCEEEHHCCCCCE
HYFTPEGVFAMQEIFGSDIIMPLDICSSYGIDYNEANLYTNITTNWARSTFKAYKNRKEG
EEECCCHHHHHHHHHCCCCEEHHHHHHHCCCCCCCCEEEEEEECHHHHHHHHHHHHHCCC
YNGLLFLITQGNFFKDLRKRSINDILELDSPGIAIGGISVGEPREKYLEILEYSSLLMPK
CCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCC
EKPRYVMGIGTPHYILDAIYHGIDIFDCVNPARIARHGSLLTDNGIMRISRKEYKDDTSQ
CCCCEEEECCCHHHHHHHHHHCCHHHHCCCHHHHHHCCCEEECCCEEEECHHHHCCHHHH
VEKNCGCTLCTRYSRGYLRHLIKSKELFGIILASEHNIYYMFRLISKIRTAILNDNFLNF
HHHCCCCEEEHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHH
RTSYLKKYEEENFDE
HHHHHHHHHHCCCCC
>Mature Secondary Structure
MFSVIKNDKHSNARVGFLNLPHGRVDTPCFMPVGTLGAMKGLKHSVLEKLKCNLMLANTY
CCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEE
HLYLRPGIKTIEKYGGLHNFTTWNKNFLTDSGGFQVFSLSSLRKIDLKGVRFKSHLDGSY
EEEECCCHHHHHHHCCCCCCCCCCCCEEECCCCEEEEEECCCCEECCCCEEEHHCCCCCE
HYFTPEGVFAMQEIFGSDIIMPLDICSSYGIDYNEANLYTNITTNWARSTFKAYKNRKEG
EEECCCHHHHHHHHHCCCCEEHHHHHHHCCCCCCCCEEEEEEECHHHHHHHHHHHHHCCC
YNGLLFLITQGNFFKDLRKRSINDILELDSPGIAIGGISVGEPREKYLEILEYSSLLMPK
CCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCC
EKPRYVMGIGTPHYILDAIYHGIDIFDCVNPARIARHGSLLTDNGIMRISRKEYKDDTSQ
CCCCEEEECCCHHHHHHHHHHCCHHHHCCCHHHHHHCCCEEECCCEEEECHHHHCCHHHH
VEKNCGCTLCTRYSRGYLRHLIKSKELFGIILASEHNIYYMFRLISKIRTAILNDNFLNF
HHHCCCCEEEHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHH
RTSYLKKYEEENFDE
HHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9403685 [H]