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The map label for this gene is trmD [H]

Identifier: 51598952

GI number: 51598952

Start: 739626

End: 740345

Strand: Direct

Name: trmD [H]

Synonym: BG0721

Alternate gene names: 51598952

Gene position: 739626-740345 (Clockwise)

Preceding gene: 51598951

Following gene: 51598953

Centisome position: 81.79

GC content: 30.14

Gene sequence:

>720_bases
ATGAAATTTACCGTTATATCTCTTTTCCCAGCAATAATTAAACCATTTTTTGAAAATTCAATAATGAAAAAAGCTATTAA
CAAGGGAATAGTAAGTTTTGAGCTTATTGATGTTAGAGATTTTTCAAAAGATAAACATAAAAGGTGTGACGATTTGTCTT
ACGGAGGCGGTGCTGGGATGGTGTTAAAAGCTGAGCCCATGTCTTTTGCTCTTGAGCATGTCGAAGCTACTAAGAAAACA
ACAATATTTTTAAGTCCTTCTGGTATAAAGTATACTCAAGAATTGGCATATTCCTTGTCAAAAAAAGAAGAAATTGTTAT
AATTTGTGGAAGATATGAAGGAATTGATCAACGTATTATAGATTTATATGTTGATTTTGAGATTTCTATTGGAGATTATG
TTCTATCTTCAGGGGAGATTGCAGCTCTTGTTTTAATAGATAGTGTATATAGGTTGCTAGATGGAGTAATAAATCCTAAT
TCTTTATTGGAAGAATCATTTGGGATGAAAAATGGGTTACTTGAATATCCTCATTACACCAGACCTTATAATTTTATGGG
GATAAAGGTTCCAGAAGTTCTTGTTTCAGGACATCATGAAAATATAAAGAATTGGAGACTTTTTAAAGCTAGAGAAAAAA
CTAAGAAAAATAGATATGATTTATACCTTAAATATTTAGAGATAATAGGAGAAGATAATGGATTTGATAAGAAAAATTGA

Upstream 100 bases:

>100_bases
TGGGTATTAAATTTTTTTTTATTCCCTTTTTGAGCATTTATATTGGAGATATAGATGCAAGAGAAAAAACAATTGAGCTT
AAGGTTTTAGATCTTTTAAG

Downstream 100 bases:

>100_bases
AGCCCAGAATAAGAAAAGCGAGGCTTTTGTCTTTAATGTGGGGGATACTGTAAGGGTTATTTATAAAATTATTGAAGGTA
GTAATGAAAGGTTGCAGAGT

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MKFTVISLFPAIIKPFFENSIMKKAINKGIVSFELIDVRDFSKDKHKRCDDLSYGGGAGMVLKAEPMSFALEHVEATKKT
TIFLSPSGIKYTQELAYSLSKKEEIVIICGRYEGIDQRIIDLYVDFEISIGDYVLSSGEIAALVLIDSVYRLLDGVINPN
SLLEESFGMKNGLLEYPHYTRPYNFMGIKVPEVLVSGHHENIKNWRLFKAREKTKKNRYDLYLKYLEIIGEDNGFDKKN

Sequences:

>Translated_239_residues
MKFTVISLFPAIIKPFFENSIMKKAINKGIVSFELIDVRDFSKDKHKRCDDLSYGGGAGMVLKAEPMSFALEHVEATKKT
TIFLSPSGIKYTQELAYSLSKKEEIVIICGRYEGIDQRIIDLYVDFEISIGDYVLSSGEIAALVLIDSVYRLLDGVINPN
SLLEESFGMKNGLLEYPHYTRPYNFMGIKVPEVLVSGHHENIKNWRLFKAREKTKKNRYDLYLKYLEIIGEDNGFDKKN
>Mature_239_residues
MKFTVISLFPAIIKPFFENSIMKKAINKGIVSFELIDVRDFSKDKHKRCDDLSYGGGAGMVLKAEPMSFALEHVEATKKT
TIFLSPSGIKYTQELAYSLSKKEEIVIICGRYEGIDQRIIDLYVDFEISIGDYVLSSGEIAALVLIDSVYRLLDGVINPN
SLLEESFGMKNGLLEYPHYTRPYNFMGIKVPEVLVSGHHENIKNWRLFKAREKTKKNRYDLYLKYLEIIGEDNGFDKKN

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=225, Percent_Identity=40.4444444444444, Blast_Score=179, Evalue=1e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 27328; Mature: 27328

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: PS00211 ABC_TRANSPORTER_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFTVISLFPAIIKPFFENSIMKKAINKGIVSFELIDVRDFSKDKHKRCDDLSYGGGAGM
CEEEHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECHHCCCHHHCCCCCCCCCCCCCE
VLKAEPMSFALEHVEATKKTTIFLSPSGIKYTQELAYSLSKKEEIVIICGRYEGIDQRII
EEEECHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCEEE
DLYVDFEISIGDYVLSSGEIAALVLIDSVYRLLDGVINPNSLLEESFGMKNGLLEYPHYT
EEEEEEEEECCCEEECCCCEEEHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCC
RPYNFMGIKVPEVLVSGHHENIKNWRLFKAREKTKKNRYDLYLKYLEIIGEDNGFDKKN
CCCCEEEEEHHHHHHCCCCCCCCCEEEEHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKFTVISLFPAIIKPFFENSIMKKAINKGIVSFELIDVRDFSKDKHKRCDDLSYGGGAGM
CEEEHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECHHCCCHHHCCCCCCCCCCCCCE
VLKAEPMSFALEHVEATKKTTIFLSPSGIKYTQELAYSLSKKEEIVIICGRYEGIDQRII
EEEECHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCEEE
DLYVDFEISIGDYVLSSGEIAALVLIDSVYRLLDGVINPNSLLEESFGMKNGLLEYPHYT
EEEEEEEEECCCEEECCCCEEEHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCC
RPYNFMGIKVPEVLVSGHHENIKNWRLFKAREKTKKNRYDLYLKYLEIIGEDNGFDKKN
CCCCEEEEEHHHHHHCCCCCCCCCEEEEHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9403685 [H]