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The map label for this gene is exoA [H]

Identifier: 51598785

GI number: 51598785

Start: 545228

End: 545995

Strand: Direct

Name: exoA [H]

Synonym: BG0542

Alternate gene names: 51598785

Gene position: 545228-545995 (Clockwise)

Preceding gene: 51598784

Following gene: 51598786

Centisome position: 60.3

GC content: 29.69

Gene sequence:

>768_bases
ATGAAATTAATTTCATGGAATGTAAATGGAATTAGAGCTGTTTTAAAGAAAGGTTTTCTTGAGTTTGTAAAAGAATATAC
CCCAGATATTTTATGTGTTCAAGAAACTAAAGCTTTGAAAGAGCAGTTGCCAAGGGATTTAATTCTTGAAAATTATTATT
CTTATTTTTCAAAGTCAAAGATTAAAGGTTATAGTGGTGTTTGTATTTACTCTAAAGTTAAGCCTATTTCTGTAAATTTA
CTTGGAAAAGAAATCTTTGACAATGAAGGTAGGGGGCTTATAGCATACTATGATAATTTTGTATTGATTAACGGTTATTT
TCCTAATTCTCAAGCTTTAAGAAGAAGGCTTGTTTATAAACTTAATTTTTTATCTTATGTTGAGAATCTTATAGATTCTC
TTGTAAATGGTGGGAAAAATGTAGTAATTTGTGGTGATTTTAATATTGCTCATACCAAGATTGACCTTATAAGTCCTGAT
TCTAATAGAGACTCTCCCGGATATTATATTGAAGAAACGACTTGGCTAGATAGTTTTTTAAACAAAGGATATGTGGATAC
ATTTAGGATATTTAATAAGGAACCCGGTTATTATACTTGGTGGAGCTATAGAACAAGAGCAAGGGAGAGAAATATGGGTT
GGAGAATTGATTATTTTGTTGTGAATGAATTTTTTAAGAGAAATGTTAAAAAATCTCTAATTTTAGACAAAGTAATGGGA
AGCGACCATTGTCCTGTTTTTTTAGAGTTGGCTGATGTAGTTAGTTAA

Upstream 100 bases:

>100_bases
ATATAATGCATGAAGAATTTGATTATTTAGAAAAAGACAATATCTATTTAGCTTATGATGGATTAAAGATTTATTTTTGA
TTTAAATTTTGAGAGGATTT

Downstream 100 bases:

>100_bases
AGGGAGGAAGCTAGATTGAAAAAAAATATTGCAATTTTTTATTTAATATTTAGTGCTATTTTTAGTTTTGGCATTAATTT
AAATGATTTTGATTTTTACC

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MKLISWNVNGIRAVLKKGFLEFVKEYTPDILCVQETKALKEQLPRDLILENYYSYFSKSKIKGYSGVCIYSKVKPISVNL
LGKEIFDNEGRGLIAYYDNFVLINGYFPNSQALRRRLVYKLNFLSYVENLIDSLVNGGKNVVICGDFNIAHTKIDLISPD
SNRDSPGYYIEETTWLDSFLNKGYVDTFRIFNKEPGYYTWWSYRTRARERNMGWRIDYFVVNEFFKRNVKKSLILDKVMG
SDHCPVFLELADVVS

Sequences:

>Translated_255_residues
MKLISWNVNGIRAVLKKGFLEFVKEYTPDILCVQETKALKEQLPRDLILENYYSYFSKSKIKGYSGVCIYSKVKPISVNL
LGKEIFDNEGRGLIAYYDNFVLINGYFPNSQALRRRLVYKLNFLSYVENLIDSLVNGGKNVVICGDFNIAHTKIDLISPD
SNRDSPGYYIEETTWLDSFLNKGYVDTFRIFNKEPGYYTWWSYRTRARERNMGWRIDYFVVNEFFKRNVKKSLILDKVMG
SDHCPVFLELADVVS
>Mature_255_residues
MKLISWNVNGIRAVLKKGFLEFVKEYTPDILCVQETKALKEQLPRDLILENYYSYFSKSKIKGYSGVCIYSKVKPISVNL
LGKEIFDNEGRGLIAYYDNFVLINGYFPNSQALRRRLVYKLNFLSYVENLIDSLVNGGKNVVICGDFNIAHTKIDLISPD
SNRDSPGYYIEETTWLDSFLNKGYVDTFRIFNKEPGYYTWWSYRTRARERNMGWRIDYFVVNEFFKRNVKKSLILDKVMG
SDHCPVFLELADVVS

Specific function: Major Apurinic-Apyrimidinic Endonuclease Of E.Coli. It Removes The Damaged DNA At Cytosines And Guanines By Cleaving On The 3' Side Of The Ap Site By A Beta-Elimination Reaction. It Exhibits 3'-5'-Exonuclease, 3'-Phosphomonoesterase, 3'-Repair Diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=259, Percent_Identity=38.6100386100386, Blast_Score=181, Evalue=6e-46,
Organism=Homo sapiens, GI18375503, Length=259, Percent_Identity=38.6100386100386, Blast_Score=181, Evalue=6e-46,
Organism=Homo sapiens, GI18375501, Length=259, Percent_Identity=38.6100386100386, Blast_Score=181, Evalue=6e-46,
Organism=Homo sapiens, GI18375507, Length=316, Percent_Identity=30.379746835443, Blast_Score=125, Evalue=5e-29,
Organism=Escherichia coli, GI1788046, Length=266, Percent_Identity=28.1954887218045, Blast_Score=106, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI71989536, Length=253, Percent_Identity=35.1778656126482, Blast_Score=158, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI71989539, Length=115, Percent_Identity=38.2608695652174, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6319452, Length=204, Percent_Identity=27.4509803921569, Blast_Score=64, Evalue=2e-11,
Organism=Drosophila melanogaster, GI221330655, Length=255, Percent_Identity=43.1372549019608, Blast_Score=194, Evalue=6e-50,
Organism=Drosophila melanogaster, GI17136678, Length=255, Percent_Identity=43.1372549019608, Blast_Score=193, Evalue=9e-50,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29764; Mature: 29764

Theoretical pI: Translated: 9.10; Mature: 9.10

Prosite motif: PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLISWNVNGIRAVLKKGFLEFVKEYTPDILCVQETKALKEQLPRDLILENYYSYFSKSK
CEEEEECHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHCCHHHHHHHHHHHHHHHH
IKGYSGVCIYSKVKPISVNLLGKEIFDNEGRGLIAYYDNFVLINGYFPNSQALRRRLVYK
CCCCCCEEEEECCCEEEEEEECHHHHCCCCCEEEEEECCEEEEEEECCCHHHHHHHHHHH
LNFLSYVENLIDSLVNGGKNVVICGDFNIAHTKIDLISPDSNRDSPGYYIEETTWLDSFL
HHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEEEEECCCCCCCCCCEEEEHHHHHHHHH
NKGYVDTFRIFNKEPGYYTWWSYRTRARERNMGWRIDYFVVNEFFKRNVKKSLILDKVMG
CCCCHHHHHEECCCCCEEEEEHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHC
SDHCPVFLELADVVS
CCCCCHHEEHHHHCC
>Mature Secondary Structure
MKLISWNVNGIRAVLKKGFLEFVKEYTPDILCVQETKALKEQLPRDLILENYYSYFSKSK
CEEEEECHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHCCHHHHHHHHHHHHHHHH
IKGYSGVCIYSKVKPISVNLLGKEIFDNEGRGLIAYYDNFVLINGYFPNSQALRRRLVYK
CCCCCCEEEEECCCEEEEEEECHHHHCCCCCEEEEEECCEEEEEEECCCHHHHHHHHHHH
LNFLSYVENLIDSLVNGGKNVVICGDFNIAHTKIDLISPDSNRDSPGYYIEETTWLDSFL
HHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEEEEECCCCCCCCCCEEEEHHHHHHHHH
NKGYVDTFRIFNKEPGYYTWWSYRTRARERNMGWRIDYFVVNEFFKRNVKKSLILDKVMG
CCCCHHHHHEECCCCCEEEEEHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHC
SDHCPVFLELADVVS
CCCCCHHEEHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]