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The map label for this gene is nadE [H]

Identifier: 51598774

GI number: 51598774

Start: 534365

End: 535903

Strand: Direct

Name: nadE [H]

Synonym: BG0531

Alternate gene names: 51598774

Gene position: 534365-535903 (Clockwise)

Preceding gene: 51598769

Following gene: 51598775

Centisome position: 59.1

GC content: 29.89

Gene sequence:

>1539_bases
ATGAAAATAAGCATTGTTCAAACGAAATACAGAGTTTTAGATTTTAATCAATGTATTGATGATTTTAAAATTAATTATGA
AGAAGCCTTGCTAAGGGGATCAGAAGTTTTAGTTTTTCCTTCTATGTTTTTAGATAAGACTAAGCATAGTGAGTTGTTTG
AGCGTTCTCAATATTCTCAAAATATATGTAAGTTCATTGAGTTTATAAGAGAACAGGTTGATGATAGAATTTTAGTTGTT
TTTGGGCATAGTGTTTATAAGGGTGGCGAATTTGTAAATATTATTTCTGTAATCAGTAATCATAAAGTTATTCTAACTAT
ATCACAATGCAACTTTCCCGGGTTTTTTAGTTATAAGGACAATGTATTTGCGGTATTAAATTTTGAAGATGCTTTATTGT
TTAATGAATTAAGTCCCAAATTTGGTGAGAATTTAAAGAAGGCTCAGTACTTAATAGTACCATCCAAATCTTATTTTACT
AAGGAAAAGAATAATCTTAGGTTAAAATTCTTTGAAAGAGTTGCTGTTGAAAACAATCTAGATGTTGTTTATTCAAATTT
TTATGGGGCTGAAGATTCTGCAGTTTATGATGGGTGTAGCTTTTTTATTAATAGTTTTGGGAAGATAAAACAAGCCAAAG
GATTTGAATTTGATTTTATATCTAATGATGCATTTCAAGATTTAAAATTTGATACATATAATGATTTTTTTGAAAAAATT
ATTATAGCAATATCTCTTGTCTTAAGAGAATATGTTCGTTTTTCAGGATTTTCTAAAGTGCATTTGGGGGTATCTGGGGG
TATTGATTCTGCCCTTGTTGCGTGTCTTGCATGTTTTGCTTTGGGCGCTGATAATGTTGTTGGAATTTCTATGCCTAGTA
AATTTTCATCAACAGGCTCTATTTCTGATGCTAAAGAGCTTTCTAGAAAATTAGGTTTTAAATTGATTGAAATGCCAATA
AAAGACTTGTTTGAGGTAAGCAGTAGATTTCTTGAAGGGCATTTTGATGTTAAAGGCGTTACCGAAGAAAATTTACAAGC
CCGGTTAAGGGGACTTTTATTAATGTCTTATAGTAATTCTCAAAATTCTTTACTTTTAAATACTGGCAATAAAAGCGAGA
TTGCAGTTGGTTATTGCACCCTTTATGGGGATGCTTGTGGGGGACTTGCCTTGATTGGAGATCTTTTTAAGACTGAGGTT
TATGATCTTGTAAAGTACATTAATGCAAAGTTTGACCAATGTATTATTCCTGTTAATATTATTTTAAAGGAGCCTTCAGC
TGAGTTAAGATTTGACCAAAAAGATAGCGATTATCTTCCAAAATATGAATTTCTTGATGTTATTTTAAGTAGATATTTGA
TTGACAATGAATCTGTAGATTCAATTTACCTTCATTTTGAAAAAGGTGTAGTCGATAAAGTTTTAACTCTATATTTTAAA
AATGAATACAAAAGAAGACAAGGAGCTCCTATAGTTAAAGTTTCTAAAAAAACCTTTGGGTTTGAACTTTCAATTCCTAT
TTTAAATAATATGATTTGA

Upstream 100 bases:

>100_bases
TCTTAATTTTAAGGTATTATTAAAATATGGATGAGTATTGGCTTTCCGAATCTGTTAAAATTTTGTTGTAATTTAATGTA
TTATTGCTTATTATTTACCT

Downstream 100 bases:

>100_bases
AATTATGTAAAGCTTAGTTTTTTAAATTCTTTTCCTTTTTTTTAAATTTTATTGATAATATTTTTTATGGGGAAAATTGG
AGATTTAATATTATTTAATG

Product: NH(3)-dependent NAD+ synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 512; Mature: 512

Protein sequence:

>512_residues
MKISIVQTKYRVLDFNQCIDDFKINYEEALLRGSEVLVFPSMFLDKTKHSELFERSQYSQNICKFIEFIREQVDDRILVV
FGHSVYKGGEFVNIISVISNHKVILTISQCNFPGFFSYKDNVFAVLNFEDALLFNELSPKFGENLKKAQYLIVPSKSYFT
KEKNNLRLKFFERVAVENNLDVVYSNFYGAEDSAVYDGCSFFINSFGKIKQAKGFEFDFISNDAFQDLKFDTYNDFFEKI
IIAISLVLREYVRFSGFSKVHLGVSGGIDSALVACLACFALGADNVVGISMPSKFSSTGSISDAKELSRKLGFKLIEMPI
KDLFEVSSRFLEGHFDVKGVTEENLQARLRGLLLMSYSNSQNSLLLNTGNKSEIAVGYCTLYGDACGGLALIGDLFKTEV
YDLVKYINAKFDQCIIPVNIILKEPSAELRFDQKDSDYLPKYEFLDVILSRYLIDNESVDSIYLHFEKGVVDKVLTLYFK
NEYKRRQGAPIVKVSKKTFGFELSIPILNNMI

Sequences:

>Translated_512_residues
MKISIVQTKYRVLDFNQCIDDFKINYEEALLRGSEVLVFPSMFLDKTKHSELFERSQYSQNICKFIEFIREQVDDRILVV
FGHSVYKGGEFVNIISVISNHKVILTISQCNFPGFFSYKDNVFAVLNFEDALLFNELSPKFGENLKKAQYLIVPSKSYFT
KEKNNLRLKFFERVAVENNLDVVYSNFYGAEDSAVYDGCSFFINSFGKIKQAKGFEFDFISNDAFQDLKFDTYNDFFEKI
IIAISLVLREYVRFSGFSKVHLGVSGGIDSALVACLACFALGADNVVGISMPSKFSSTGSISDAKELSRKLGFKLIEMPI
KDLFEVSSRFLEGHFDVKGVTEENLQARLRGLLLMSYSNSQNSLLLNTGNKSEIAVGYCTLYGDACGGLALIGDLFKTEV
YDLVKYINAKFDQCIIPVNIILKEPSAELRFDQKDSDYLPKYEFLDVILSRYLIDNESVDSIYLHFEKGVVDKVLTLYFK
NEYKRRQGAPIVKVSKKTFGFELSIPILNNMI
>Mature_512_residues
MKISIVQTKYRVLDFNQCIDDFKINYEEALLRGSEVLVFPSMFLDKTKHSELFERSQYSQNICKFIEFIREQVDDRILVV
FGHSVYKGGEFVNIISVISNHKVILTISQCNFPGFFSYKDNVFAVLNFEDALLFNELSPKFGENLKKAQYLIVPSKSYFT
KEKNNLRLKFFERVAVENNLDVVYSNFYGAEDSAVYDGCSFFINSFGKIKQAKGFEFDFISNDAFQDLKFDTYNDFFEKI
IIAISLVLREYVRFSGFSKVHLGVSGGIDSALVACLACFALGADNVVGISMPSKFSSTGSISDAKELSRKLGFKLIEMPI
KDLFEVSSRFLEGHFDVKGVTEENLQARLRGLLLMSYSNSQNSLLLNTGNKSEIAVGYCTLYGDACGGLALIGDLFKTEV
YDLVKYINAKFDQCIIPVNIILKEPSAELRFDQKDSDYLPKYEFLDVILSRYLIDNESVDSIYLHFEKGVVDKVLTLYFK
NEYKRRQGAPIVKVSKKTFGFELSIPILNNMI

Specific function: It is not known if this NAD synthase uses NH(3) or glutamine as the N donor [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 58315; Mature: 58315

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKISIVQTKYRVLDFNQCIDDFKINYEEALLRGSEVLVFPSMFLDKTKHSELFERSQYSQ
CEEEEEEHHHHEEEHHHHHHHHCCCHHHHHHCCCEEEEECHHHHCCCHHHHHHHHHHHHH
NICKFIEFIREQVDDRILVVFGHSVYKGGEFVNIISVISNHKVILTISQCNFPGFFSYKD
HHHHHHHHHHHHCCCEEEEEECCCHHCCCCHHHHHHHHCCCEEEEEEECCCCCCEECCCC
NVFAVLNFEDALLFNELSPKFGENLKKAQYLIVPSKSYFTKEKNNLRLKFFERVAVENNL
CEEEEEECCHHHHHHHCCCHHCCCCCCCEEEEEECCHHHCCCCCCCEEEEEHHHHHCCCC
DVVYSNFYGAEDSAVYDGCSFFINSFGKIKQAKGFEFDFISNDAFQDLKFDTYNDFFEKI
EEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHCCCCCHHHHHHHHH
IIAISLVLREYVRFSGFSKVHLGVSGGIDSALVACLACFALGADNVVGISMPSKFSSTGS
HHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCC
ISDAKELSRKLGFKLIEMPIKDLFEVSSRFLEGHFDVKGVTEENLQARLRGLLLMSYSNS
CCHHHHHHHHHCCCEEHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHEEEEEEECCC
QNSLLLNTGNKSEIAVGYCTLYGDACGGLALIGDLFKTEVYDLVKYINAKFDQCIIPVNI
CCEEEEECCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEE
ILKEPSAELRFDQKDSDYLPKYEFLDVILSRYLIDNESVDSIYLHFEKGVVDKVLTLYFK
EEECCCCCCEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHH
NEYKRRQGAPIVKVSKKTFGFELSIPILNNMI
HHHHHHCCCCEEEEECCCCCEEEECHHHCCCC
>Mature Secondary Structure
MKISIVQTKYRVLDFNQCIDDFKINYEEALLRGSEVLVFPSMFLDKTKHSELFERSQYSQ
CEEEEEEHHHHEEEHHHHHHHHCCCHHHHHHCCCEEEEECHHHHCCCHHHHHHHHHHHHH
NICKFIEFIREQVDDRILVVFGHSVYKGGEFVNIISVISNHKVILTISQCNFPGFFSYKD
HHHHHHHHHHHHCCCEEEEEECCCHHCCCCHHHHHHHHCCCEEEEEEECCCCCCEECCCC
NVFAVLNFEDALLFNELSPKFGENLKKAQYLIVPSKSYFTKEKNNLRLKFFERVAVENNL
CEEEEEECCHHHHHHHCCCHHCCCCCCCEEEEEECCHHHCCCCCCCEEEEEHHHHHCCCC
DVVYSNFYGAEDSAVYDGCSFFINSFGKIKQAKGFEFDFISNDAFQDLKFDTYNDFFEKI
EEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHCCCCCHHHHHHHHH
IIAISLVLREYVRFSGFSKVHLGVSGGIDSALVACLACFALGADNVVGISMPSKFSSTGS
HHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCC
ISDAKELSRKLGFKLIEMPIKDLFEVSSRFLEGHFDVKGVTEENLQARLRGLLLMSYSNS
CCHHHHHHHHHCCCEEHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHEEEEEEECCC
QNSLLLNTGNKSEIAVGYCTLYGDACGGLALIGDLFKTEVYDLVKYINAKFDQCIIPVNI
CCEEEEECCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEE
ILKEPSAELRFDQKDSDYLPKYEFLDVILSRYLIDNESVDSIYLHFEKGVVDKVLTLYFK
EEECCCCCCEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHH
NEYKRRQGAPIVKVSKKTFGFELSIPILNNMI
HHHHHHCCCCEEEEECCCCCEEEECHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8431308; 8195100 [H]