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The map label for this gene is pheS [H]

Identifier: 51598767

GI number: 51598767

Start: 525306

End: 526868

Strand: Direct

Name: pheS [H]

Synonym: BG0524

Alternate gene names: 51598767

Gene position: 525306-526868 (Clockwise)

Preceding gene: 51598766

Following gene: 51598768

Centisome position: 58.09

GC content: 31.93

Gene sequence:

>1563_bases
ATGAAGGCAGATTTAAATTTAATAAAAACATTACATCCTCTTGAGATGAAAGTAATTTTAAACAATGGAGAAGAGGATGA
TATTTCTGCTTCAATTATTATTGAAAAATTAGGGTTTAATGAAGGTCAGGCAAACAAAACAATTGAATGGTTAAATTCTA
AAAGGATTATTGAAGAGACTTGTAGAAAATTAAATGTATTTTATAAAGCAACAGAAAGAGGCCTTAGCGCTTTAATAGCT
GGGTTTGTTGAGGAAAAAATAATAAACCTGGTATCTCGAAAGGCAGTTTTGGCTTCAAATTTAGCCTTAGAGCTTGATCT
TGATGTTAAAGAAGTGGGAAAAGCGTTTGGAAATTTATTAAAAGAGGGCATTCTTTCTCTTGATTTGAATAAACAGATTA
TTATAAATTGTTTAGATGGCATAGAGGTCAATTATCAAAAAATACGTTTCTTGCTAGAAAGAGCAAAAAGTAGTGATCTT
CTTAGAGAAAGTTTAACAAATGAAGAGCTTTTATTAATATCAAATTTTGCTAAGAAAAAAGGAGCAGATTCTGTATTTTT
TAAAATAATAGAAAAACTTGATTTAACGTTTAGATTGTCTGGTTTTGGATTGGAAGTGAAAAATTTTTTAATGAAAAATA
AATTAACAGGAGATGAGATTGCTAAGCTTACTCCTGAAATTTTAAAAAATAAGACTTATGAGAATAAAAAATTTAGAGCT
TATAATATTCATGTTCCATCAGCTAAAACTTTTATTGGGCGCTTTAATTCTTATTTAGATTATATTTCTAAAATTAAAGA
CAAATTAGTAGGGCTTGGTTTTGAAGAGTTTGACGGTCCTTTAATAGAAACAGAATTTTTCAACAACGATGCTCTTTTTA
TGCCTCAATTTCATCCTTCTCGTGATATTAAGGATGTTTATTACATTTCAGATCCCAGTATGCAAAAATCTTTACCCGAA
CCTTATTTTTCTAATGTAAAATTAGCTCATGAAACAGGATATGCAACAGGTTCAAGAGGTTGGAGATATAGTTTTAGTGA
GGATCTTTCCAAGAGATTGGTTTTAAGAACTCATGGCACCGTTCTTTCTGCAAAACAATTAATTAATGCCAAAAATCCAA
GCAGATATTTTGGAGTTATTAGATGTTTTAGATATGATCAAGTAGATGCAACTCACGGAGTCGATTTTTATCAAACCGAG
GGGATTGTTATTGAAGATGGTGTTAGCATTAAGACTTTGCTAGGCCTTCTTGAAATTTTTGCCAAAGAGTTTGCGGGCGC
TACAGAAGTTAAATATGTTCCCGCATATTTTCCTTTTACTGAGCCCTCGATTGAAATACATGTAAAACATCCTGTTCTTG
GCTGGTTTGAGCTTGGAGGAAGTGGAATTTTTAGGCCAGAAGTTACAAAACCCTTAGGTATTGATTTTCCTGTTATTGCT
TGGGGGATTGGAATAGATAGAATGGCTTTAATGCACTTGGGCCTAAATGATTTGAGAGATCTTTTTACGTATGATATTGG
TGATGTTGTTTTAAGGCGAGGAAAGAGAAGATGCCAAAGGTAG

Upstream 100 bases:

>100_bases
GTGCTGAAGAAATTTCTAGAGCTACTAAATTATCTATTGGGGAAGTTGAGCTTATTTTAGAGCTTGGTATAAGCAATAAA
GGTGATTAAGGGTTATATTT

Downstream 100 bases:

>100_bases
AAATTTACAAAAATCTTTTTTTAGATAAAATAGGAAAAAATTTTACAAATTTAGAGATTTCAGAATTACTTGAACCATTT
AAAGCAGAATTTGATGGCTT

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS [H]

Number of amino acids: Translated: 520; Mature: 520

Protein sequence:

>520_residues
MKADLNLIKTLHPLEMKVILNNGEEDDISASIIIEKLGFNEGQANKTIEWLNSKRIIEETCRKLNVFYKATERGLSALIA
GFVEEKIINLVSRKAVLASNLALELDLDVKEVGKAFGNLLKEGILSLDLNKQIIINCLDGIEVNYQKIRFLLERAKSSDL
LRESLTNEELLLISNFAKKKGADSVFFKIIEKLDLTFRLSGFGLEVKNFLMKNKLTGDEIAKLTPEILKNKTYENKKFRA
YNIHVPSAKTFIGRFNSYLDYISKIKDKLVGLGFEEFDGPLIETEFFNNDALFMPQFHPSRDIKDVYYISDPSMQKSLPE
PYFSNVKLAHETGYATGSRGWRYSFSEDLSKRLVLRTHGTVLSAKQLINAKNPSRYFGVIRCFRYDQVDATHGVDFYQTE
GIVIEDGVSIKTLLGLLEIFAKEFAGATEVKYVPAYFPFTEPSIEIHVKHPVLGWFELGGSGIFRPEVTKPLGIDFPVIA
WGIGIDRMALMHLGLNDLRDLFTYDIGDVVLRRGKRRCQR

Sequences:

>Translated_520_residues
MKADLNLIKTLHPLEMKVILNNGEEDDISASIIIEKLGFNEGQANKTIEWLNSKRIIEETCRKLNVFYKATERGLSALIA
GFVEEKIINLVSRKAVLASNLALELDLDVKEVGKAFGNLLKEGILSLDLNKQIIINCLDGIEVNYQKIRFLLERAKSSDL
LRESLTNEELLLISNFAKKKGADSVFFKIIEKLDLTFRLSGFGLEVKNFLMKNKLTGDEIAKLTPEILKNKTYENKKFRA
YNIHVPSAKTFIGRFNSYLDYISKIKDKLVGLGFEEFDGPLIETEFFNNDALFMPQFHPSRDIKDVYYISDPSMQKSLPE
PYFSNVKLAHETGYATGSRGWRYSFSEDLSKRLVLRTHGTVLSAKQLINAKNPSRYFGVIRCFRYDQVDATHGVDFYQTE
GIVIEDGVSIKTLLGLLEIFAKEFAGATEVKYVPAYFPFTEPSIEIHVKHPVLGWFELGGSGIFRPEVTKPLGIDFPVIA
WGIGIDRMALMHLGLNDLRDLFTYDIGDVVLRRGKRRCQR
>Mature_520_residues
MKADLNLIKTLHPLEMKVILNNGEEDDISASIIIEKLGFNEGQANKTIEWLNSKRIIEETCRKLNVFYKATERGLSALIA
GFVEEKIINLVSRKAVLASNLALELDLDVKEVGKAFGNLLKEGILSLDLNKQIIINCLDGIEVNYQKIRFLLERAKSSDL
LRESLTNEELLLISNFAKKKGADSVFFKIIEKLDLTFRLSGFGLEVKNFLMKNKLTGDEIAKLTPEILKNKTYENKKFRA
YNIHVPSAKTFIGRFNSYLDYISKIKDKLVGLGFEEFDGPLIETEFFNNDALFMPQFHPSRDIKDVYYISDPSMQKSLPE
PYFSNVKLAHETGYATGSRGWRYSFSEDLSKRLVLRTHGTVLSAKQLINAKNPSRYFGVIRCFRYDQVDATHGVDFYQTE
GIVIEDGVSIKTLLGLLEIFAKEFAGATEVKYVPAYFPFTEPSIEIHVKHPVLGWFELGGSGIFRPEVTKPLGIDFPVIA
WGIGIDRMALMHLGLNDLRDLFTYDIGDVVLRRGKRRCQR

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758340, Length=295, Percent_Identity=35.5932203389831, Blast_Score=200, Evalue=3e-51,
Organism=Escherichia coli, GI1788007, Length=269, Percent_Identity=27.5092936802974, Blast_Score=109, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI32563657, Length=294, Percent_Identity=36.734693877551, Blast_Score=206, Evalue=4e-53,
Organism=Caenorhabditis elegans, GI17508957, Length=294, Percent_Identity=36.734693877551, Blast_Score=205, Evalue=4e-53,
Organism=Saccharomyces cerevisiae, GI6321087, Length=457, Percent_Identity=29.7592997811816, Blast_Score=192, Evalue=9e-50,
Organism=Drosophila melanogaster, GI18858079, Length=292, Percent_Identity=35.2739726027397, Blast_Score=186, Evalue=4e-47,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR022917
- InterPro:   IPR002319 [H]

Pfam domain/function: PF01409 tRNA-synt_2d [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 59096; Mature: 59096

Theoretical pI: Translated: 8.26; Mature: 8.26

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKADLNLIKTLHPLEMKVILNNGEEDDISASIIIEKLGFNEGQANKTIEWLNSKRIIEET
CCCCHHHHHHCCCCEEEEEECCCCCCCCHHEEEEEECCCCCCCCCCHHHHHCCCHHHHHH
CRKLNVFYKATERGLSALIAGFVEEKIINLVSRKAVLASNLALELDLDVKEVGKAFGNLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHH
KEGILSLDLNKQIIINCLDGIEVNYQKIRFLLERAKSSDLLRESLTNEELLLISNFAKKK
HHHHEEECCCHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHC
GADSVFFKIIEKLDLTFRLSGFGLEVKNFLMKNKLTGDEIAKLTPEILKNKTYENKKFRA
CCHHHHHHHHHHHCCEEEEECCCHHHHHHHHHCCCCHHHHHHHCHHHHCCCCCCCCCEEE
YNIHVPSAKTFIGRFNSYLDYISKIKDKLVGLGFEEFDGPLIETEFFNNDALFMPQFHPS
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCEEEEEEECCCEEECCCCCCC
RDIKDVYYISDPSMQKSLPEPYFSNVKLAHETGYATGSRGWRYSFSEDLSKRLVLRTHGT
CCCCEEEEECCCCHHHCCCCCHHCCEEEEECCCCCCCCCCCCCCHHHHHHHHEEHEECCC
VLSAKQLINAKNPSRYFGVIRCFRYDQVDATHGVDFYQTEGIVIEDGVSIKTLLGLLEIF
HHHHHHHHCCCCCHHHHHHHHHEECCCCCCCCCCCEEECCCEEEECCCCHHHHHHHHHHH
AKEFAGATEVKYVPAYFPFTEPSIEIHVKHPVLGWFELGGSGIFRPEVTKPLGIDFPVIA
HHHHCCCCCEEEEEEECCCCCCCEEEEECCCCEEEEEECCCCCCCCCCCCCCCCCCCHHH
WGIGIDRMALMHLGLNDLRDLFTYDIGDVVLRRGKRRCQR
HHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKADLNLIKTLHPLEMKVILNNGEEDDISASIIIEKLGFNEGQANKTIEWLNSKRIIEET
CCCCHHHHHHCCCCEEEEEECCCCCCCCHHEEEEEECCCCCCCCCCHHHHHCCCHHHHHH
CRKLNVFYKATERGLSALIAGFVEEKIINLVSRKAVLASNLALELDLDVKEVGKAFGNLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHH
KEGILSLDLNKQIIINCLDGIEVNYQKIRFLLERAKSSDLLRESLTNEELLLISNFAKKK
HHHHEEECCCHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHC
GADSVFFKIIEKLDLTFRLSGFGLEVKNFLMKNKLTGDEIAKLTPEILKNKTYENKKFRA
CCHHHHHHHHHHHCCEEEEECCCHHHHHHHHHCCCCHHHHHHHCHHHHCCCCCCCCCEEE
YNIHVPSAKTFIGRFNSYLDYISKIKDKLVGLGFEEFDGPLIETEFFNNDALFMPQFHPS
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCEEEEEEECCCEEECCCCCCC
RDIKDVYYISDPSMQKSLPEPYFSNVKLAHETGYATGSRGWRYSFSEDLSKRLVLRTHGT
CCCCEEEEECCCCHHHCCCCCHHCCEEEEECCCCCCCCCCCCCCHHHHHHHHEEHEECCC
VLSAKQLINAKNPSRYFGVIRCFRYDQVDATHGVDFYQTEGIVIEDGVSIKTLLGLLEIF
HHHHHHHHCCCCCHHHHHHHHHEECCCCCCCCCCCEEECCCEEEECCCCHHHHHHHHHHH
AKEFAGATEVKYVPAYFPFTEPSIEIHVKHPVLGWFELGGSGIFRPEVTKPLGIDFPVIA
HHHHCCCCCEEEEEEECCCCCCCEEEEECCCCEEEEEECCCCCCCCCCCCCCCCCCCHHH
WGIGIDRMALMHLGLNDLRDLFTYDIGDVVLRRGKRRCQR
HHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9403685 [H]