Click here to switch to the map view.

The map label for this gene is cysG

Identifier: 51598033

GI number: 51598033

Start: 4442876

End: 4444297

Strand: Direct

Name: cysG

Synonym: YPTB3743

Alternate gene names: 51598033

Gene position: 4442876-4444297 (Clockwise)

Preceding gene: 51598032

Following gene: 229310100

Centisome position: 93.64

GC content: 53.38

Gene sequence:

>1422_bases
ATGGACTACTTCCCGATCTTCTGCCAACTGCAACACAAAGCCTGCCTGCTGGTTGGCGGTGGAGAAATTGCTGAACGCAA
AGCGCGGCTGTTATTGGATGCAGGTGCGTTAGTCACCGTCAATGCCTGCGAGTTCACCCCCCAGTTTCACCATTGGGCAG
ATCAGGGGCAACTTTCATTAATCAGCGGTGAATTTGTGCCCGAACTGTTGGCCGATAAATGGCTGGTCATTGCCGCCACC
GATCAGTTGTCCGTCAATGCATTAGTCTATCAAAGCGCCAACCAGCAACGGATCTTCTGCAATGTGGTTGATGATCCGAA
ACGCACCAGCTTTATCATGCCATCTATTATTGATCGCTCCCCCATCATGATCGCGGTCTCCTCTGGCGGTAAAGCGCCCG
TGTTGGCCCGGTTGTTGCGCGAAAAGCTGGAAGCCCTGTTACCGCAACATTTGGGGCAACTGGCGCAACTGGCGGGGAAC
TTACGCCAGCGGGTGAAGCAGCATTTTGCCGCGATGACCGAACGTCGCCGGTTCTGGGAAAAGTTGCTGACCCACGACCG
TCTGGCACAATCTTTAGCCAATAACGATCACGTGCAAGCCGATCAACATGTTGAACAACTGTTTAGTGCTCCACTCACTG
ATCGTGGCGAAGTGGTGTTGGTCGGTGCAGGGCCGGGAGATGCTGGTTTATTGACCTTAAAAGGGTTGCAGCAGATCCAA
CAGGCCGATGTGGTGGTCTATGACCGGCTGGTCTCCGATGAGGTGATGAATTTGGTCCGGCGTGATGCCGAGCGCATCTT
TGTCGGCAAACAATCGGGCCACCACTGTGTGCCGCAAGAGCAAATCAACCAAATCCTGTTACAGCAGGCACAGTCAGGTA
AGCGGGTAGTGCGTTTGAAAGGTGGCGACCCGTTTATTTTTGGTCGGGGCGGCGAAGAGTTAGAAGAGTTGGCGGGCTAC
GGTATTCCTTTCTCCGTGGTCCCAGGCATTACCGCCGCATCCGGTTGCTCGGCTTACAGTGGTATTCCACTGACTCACCG
TGACCATGCGCAAAGCGTGCGACTGGTCACCGGTCATGCCAAAAAAGAGGGCCAGTTAGATTGGGCAAATCTGGCGGCAG
AGAAACAAACATTAGTTTTTTATATGGGGCTATCGCAGGCTGGAGAGATCCAACAGCAATTGATTCAACATGGTATGCCA
GCGACCACTCAGGTTGCCTTAGTCGAAAACGGCACCTCACGGCATCAGCGGGTTGTGAGTGGCGAGTTAAGCCAATTGGC
CTTACTCTCCCAACAGGTCAGTAGCCCTAGCCTGATTATCGTCGGTAGCGTGGTCAGCCTACGGGAAAAACTGAATTGGT
TTTCCAGCCGCCATCACGATGATCAGCCCAAAGTGACTGAATGTGTGGCTCATGTTGGTTAA

Upstream 100 bases:

>100_bases
CGGCAACGATTAACGCCCCACACAGCGCAAAATCATAATAATTTGGCAGCGCTGTGATAACTCGTTGCCCTGCAACAGAC
TGAAAACTGAAGGATTGCCG

Downstream 100 bases:

>100_bases
GGTTAGCGGTGTAGTTAAGGTTAGCTATGCAGTTAAGGTTAGCTATGCAGTTAAGGCTGGCTGTGTGAGTAATATGGGTT
AATGCTGATTAATGAGGGGG

Product: siroheme synthase

Products: NA

Alternate protein names: Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM; Precorrin-2 dehydrogenase; Sirohydrochlorin ferrochelatase [H]

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MDYFPIFCQLQHKACLLVGGGEIAERKARLLLDAGALVTVNACEFTPQFHHWADQGQLSLISGEFVPELLADKWLVIAAT
DQLSVNALVYQSANQQRIFCNVVDDPKRTSFIMPSIIDRSPIMIAVSSGGKAPVLARLLREKLEALLPQHLGQLAQLAGN
LRQRVKQHFAAMTERRRFWEKLLTHDRLAQSLANNDHVQADQHVEQLFSAPLTDRGEVVLVGAGPGDAGLLTLKGLQQIQ
QADVVVYDRLVSDEVMNLVRRDAERIFVGKQSGHHCVPQEQINQILLQQAQSGKRVVRLKGGDPFIFGRGGEELEELAGY
GIPFSVVPGITAASGCSAYSGIPLTHRDHAQSVRLVTGHAKKEGQLDWANLAAEKQTLVFYMGLSQAGEIQQQLIQHGMP
ATTQVALVENGTSRHQRVVSGELSQLALLSQQVSSPSLIIVGSVVSLREKLNWFSSRHHDDQPKVTECVAHVG

Sequences:

>Translated_473_residues
MDYFPIFCQLQHKACLLVGGGEIAERKARLLLDAGALVTVNACEFTPQFHHWADQGQLSLISGEFVPELLADKWLVIAAT
DQLSVNALVYQSANQQRIFCNVVDDPKRTSFIMPSIIDRSPIMIAVSSGGKAPVLARLLREKLEALLPQHLGQLAQLAGN
LRQRVKQHFAAMTERRRFWEKLLTHDRLAQSLANNDHVQADQHVEQLFSAPLTDRGEVVLVGAGPGDAGLLTLKGLQQIQ
QADVVVYDRLVSDEVMNLVRRDAERIFVGKQSGHHCVPQEQINQILLQQAQSGKRVVRLKGGDPFIFGRGGEELEELAGY
GIPFSVVPGITAASGCSAYSGIPLTHRDHAQSVRLVTGHAKKEGQLDWANLAAEKQTLVFYMGLSQAGEIQQQLIQHGMP
ATTQVALVENGTSRHQRVVSGELSQLALLSQQVSSPSLIIVGSVVSLREKLNWFSSRHHDDQPKVTECVAHVG
>Mature_473_residues
MDYFPIFCQLQHKACLLVGGGEIAERKARLLLDAGALVTVNACEFTPQFHHWADQGQLSLISGEFVPELLADKWLVIAAT
DQLSVNALVYQSANQQRIFCNVVDDPKRTSFIMPSIIDRSPIMIAVSSGGKAPVLARLLREKLEALLPQHLGQLAQLAGN
LRQRVKQHFAAMTERRRFWEKLLTHDRLAQSLANNDHVQADQHVEQLFSAPLTDRGEVVLVGAGPGDAGLLTLKGLQQIQ
QADVVVYDRLVSDEVMNLVRRDAERIFVGKQSGHHCVPQEQINQILLQQAQSGKRVVRLKGGDPFIFGRGGEELEELAGY
GIPFSVVPGITAASGCSAYSGIPLTHRDHAQSVRLVTGHAKKEGQLDWANLAAEKQTLVFYMGLSQAGEIQQQLIQHGMP
ATTQVALVENGTSRHQRVVSGELSQLALLSQQVSSPSLIIVGSVVSLREKLNWFSSRHHDDQPKVTECVAHVG

Specific function: Multifunctional enzyme that catalyzes the SAM-dependent methylation of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 and then position C-12 or C-18 to form trimethylpyrrocorphin 2. It also catalyzes the conversion of precorrin-2 into si

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=456, Percent_Identity=73.0263157894737, Blast_Score=691, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6322922, Length=247, Percent_Identity=35.2226720647773, Blast_Score=142, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR016040
- InterPro:   IPR019478
- InterPro:   IPR006367
- InterPro:   IPR003043 [H]

Pfam domain/function: PF10414 CysG_dimeriser; PF00590 TP_methylase [H]

EC number: =2.1.1.107; =1.3.1.76; =4.99.1.4 [H]

Molecular weight: Translated: 51919; Mature: 51919

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYFPIFCQLQHKACLLVGGGEIAERKARLLLDAGALVTVNACEFTPQFHHWADQGQLSL
CCCCCEEEEECCCEEEEECCCHHHHHHHHHEEECCCEEEEEEHCCCCHHHHHCCCCCEEE
ISGEFVPELLADKWLVIAATDQLSVNALVYQSANQQRIFCNVVDDPKRTSFIMPSIIDRS
ECCHHHHHHHHCCEEEEEECCCCEEEEEEEECCCCCEEEEEECCCCCCCCEECHHHCCCC
PIMIAVSSGGKAPVLARLLREKLEALLPQHLGQLAQLAGNLRQRVKQHFAAMTERRRFWE
CEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLLTHDRLAQSLANNDHVQADQHVEQLFSAPLTDRGEVVLVGAGPGDAGLLTLKGLQQIQ
HHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCCCEEEHHHHHHHH
QADVVVYDRLVSDEVMNLVRRDAERIFVGKQSGHHCVPQEQINQILLQQAQSGKRVVRLK
HCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCHHHHHHHHHHHHCCCCEEEEEE
GGDPFIFGRGGEELEELAGYGIPFSVVPGITAASGCSAYSGIPLTHRDHAQSVRLVTGHA
CCCEEEECCCCHHHHHHHCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCC
KKEGQLDWANLAAEKQTLVFYMGLSQAGEIQQQLIQHGMPATTQVALVENGTSRHQRVVS
CCCCCCCHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHH
GELSQLALLSQQVSSPSLIIVGSVVSLREKLNWFSSRHHDDQPKVTECVAHVG
HHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MDYFPIFCQLQHKACLLVGGGEIAERKARLLLDAGALVTVNACEFTPQFHHWADQGQLSL
CCCCCEEEEECCCEEEEECCCHHHHHHHHHEEECCCEEEEEEHCCCCHHHHHCCCCCEEE
ISGEFVPELLADKWLVIAATDQLSVNALVYQSANQQRIFCNVVDDPKRTSFIMPSIIDRS
ECCHHHHHHHHCCEEEEEECCCCEEEEEEEECCCCCEEEEEECCCCCCCCEECHHHCCCC
PIMIAVSSGGKAPVLARLLREKLEALLPQHLGQLAQLAGNLRQRVKQHFAAMTERRRFWE
CEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLLTHDRLAQSLANNDHVQADQHVEQLFSAPLTDRGEVVLVGAGPGDAGLLTLKGLQQIQ
HHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCCCEEEHHHHHHHH
QADVVVYDRLVSDEVMNLVRRDAERIFVGKQSGHHCVPQEQINQILLQQAQSGKRVVRLK
HCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCHHHHHHHHHHHHCCCCEEEEEE
GGDPFIFGRGGEELEELAGYGIPFSVVPGITAASGCSAYSGIPLTHRDHAQSVRLVTGHA
CCCEEEECCCCHHHHHHHCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCC
KKEGQLDWANLAAEKQTLVFYMGLSQAGEIQQQLIQHGMPATTQVALVENGTSRHQRVVS
CCCCCCCHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHH
GELSQLALLSQQVSSPSLIIVGSVVSLREKLNWFSSRHHDDQPKVTECVAHVG
HHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA