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The map label for this gene is rsmB [H]

Identifier: 51597957

GI number: 51597957

Start: 4379306

End: 4380595

Strand: Direct

Name: rsmB [H]

Synonym: YPTB3667

Alternate gene names: 51597957

Gene position: 4379306-4380595 (Clockwise)

Preceding gene: 51597956

Following gene: 51597958

Centisome position: 92.3

GC content: 45.97

Gene sequence:

>1290_bases
ATGAAAAATACATATAATCTCCGCAGTATCGCTGCTAAAGCAATTAGTCAGGTATTGGATCAGGGCCAATCGCTCAGTGC
CGTTTTGCCTGAATTACAAAAAAATATTTCCGATAAAGATCGCGCATTGCTGCAGGAACTGTGTTTTGGCACTCTGCGAG
TCTTACCACAGCTAGAATGGTGTATTCAACAACTTATGGCACGCCCGATGACGGGTAAACAACGTGTTTTTCATTACCTG
ATTATGGTTGGGCTCTATCAGTTGATTTACACTCGGATTCCGCCACATGCGGCGTTGGCTGAAACGGTCGAAGGCGCAAC
AGTACTGAAACGTCCACAATTAAAAGGACTTATCAATGGTGTATTACGCCAATTTCAGCGTCAACAAGTGGAATTACTGG
AACGTGCTGTAAATAACGATAGCCACTATCTGCATCCTAGTTGGTTGTTGGCCCGCATTAAACAAGCCTATCCAGCACAG
TGGCAGCAAATTTTGGATGCCAATAACCAAAGACCGCCCATGTGGCTACGTGTAAATCGCCTGCATCATTCTCGTAGTGA
ATACCTCGAACTGTTAACACAGGCTGATATTAATGCGGAGCCTCACCCTATTTATCGTGATGCTGTGCGCCTTATCACTC
CTTGTGCCGTCAATCATCTACCTGGCTTTGAACTTGGTTGGGTAACCGTACAAGATGCTTCGGCTCAAGGTTGTGTTGAC
CTACTCGATCCCCAAAATGGTGAGCAGATCCTTGATTTATGTGCAGCCCCTGGAGGTAAAACCACTCATATTCTGGAAGC
CGCACCAAAGGCTCATGTATTAGCCGTAGATATTGATGAACAGCGACTCAGTCGAGTTAAAGAGAATTTACAACGCCTGC
AATTACAAGCCGTAGTCCGTGTTGGTGATGGCCGAGCACCTGACACATGGTGCGGTGATCAGCAATTTGATCGTATTCTA
TTGGATGCACCTTGTTCTGCAACCGGTGTTATTCGCCGCCATCCAGATATTAAATGGCTGCGTCGGGACCGTGATATTTC
CGAATTGGCCCAACTACAATCAGAAATCATTGAGGCTATTTGGCCTAAACTGAAACATGGTGGAGTACTAGTTTATGCTA
CCTGTTCAATATTGCCGGAAGAGAACCAACAGCAGATTGCCGCTTTCCTACAACGGCATCCCGAAGCACAGTTGACCGAA
ACCGGTACTACAGCAGCGCCTGGTAAACAAAACCTTCCACATCCTGAGGACGGTGATGGTTTCTTCTACGCAAAGATAAT
CAAAAAATAG

Upstream 100 bases:

>100_bases
AGTGTAATCGTTCGGCTCCAATAATACCTTTCATCGCCCAATACTTCGTCATTGGGCTACTTCTCCCTTTATTTGCTGGC
GTTTGTCAGCTTTTTTGGTT

Downstream 100 bases:

>100_bases
TCTGTGGCTTTTGCTATGCCTCAAAACCTAATTTCAGTGTGAAACAGAGAACGCTATGAAAATAATTATTCTTGGGGCAG
GGCAAGTTGGCGGGACCCTG

Product: 16S rRNA methyltransferase B

Products: NA

Alternate protein names: 16S rRNA m5C967 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmB [H]

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MKNTYNLRSIAAKAISQVLDQGQSLSAVLPELQKNISDKDRALLQELCFGTLRVLPQLEWCIQQLMARPMTGKQRVFHYL
IMVGLYQLIYTRIPPHAALAETVEGATVLKRPQLKGLINGVLRQFQRQQVELLERAVNNDSHYLHPSWLLARIKQAYPAQ
WQQILDANNQRPPMWLRVNRLHHSRSEYLELLTQADINAEPHPIYRDAVRLITPCAVNHLPGFELGWVTVQDASAQGCVD
LLDPQNGEQILDLCAAPGGKTTHILEAAPKAHVLAVDIDEQRLSRVKENLQRLQLQAVVRVGDGRAPDTWCGDQQFDRIL
LDAPCSATGVIRRHPDIKWLRRDRDISELAQLQSEIIEAIWPKLKHGGVLVYATCSILPEENQQQIAAFLQRHPEAQLTE
TGTTAAPGKQNLPHPEDGDGFFYAKIIKK

Sequences:

>Translated_429_residues
MKNTYNLRSIAAKAISQVLDQGQSLSAVLPELQKNISDKDRALLQELCFGTLRVLPQLEWCIQQLMARPMTGKQRVFHYL
IMVGLYQLIYTRIPPHAALAETVEGATVLKRPQLKGLINGVLRQFQRQQVELLERAVNNDSHYLHPSWLLARIKQAYPAQ
WQQILDANNQRPPMWLRVNRLHHSRSEYLELLTQADINAEPHPIYRDAVRLITPCAVNHLPGFELGWVTVQDASAQGCVD
LLDPQNGEQILDLCAAPGGKTTHILEAAPKAHVLAVDIDEQRLSRVKENLQRLQLQAVVRVGDGRAPDTWCGDQQFDRIL
LDAPCSATGVIRRHPDIKWLRRDRDISELAQLQSEIIEAIWPKLKHGGVLVYATCSILPEENQQQIAAFLQRHPEAQLTE
TGTTAAPGKQNLPHPEDGDGFFYAKIIKK
>Mature_429_residues
MKNTYNLRSIAAKAISQVLDQGQSLSAVLPELQKNISDKDRALLQELCFGTLRVLPQLEWCIQQLMARPMTGKQRVFHYL
IMVGLYQLIYTRIPPHAALAETVEGATVLKRPQLKGLINGVLRQFQRQQVELLERAVNNDSHYLHPSWLLARIKQAYPAQ
WQQILDANNQRPPMWLRVNRLHHSRSEYLELLTQADINAEPHPIYRDAVRLITPCAVNHLPGFELGWVTVQDASAQGCVD
LLDPQNGEQILDLCAAPGGKTTHILEAAPKAHVLAVDIDEQRLSRVKENLQRLQLQAVVRVGDGRAPDTWCGDQQFDRIL
LDAPCSATGVIRRHPDIKWLRRDRDISELAQLQSEIIEAIWPKLKHGGVLVYATCSILPEENQQQIAAFLQRHPEAQLTE
TGTTAAPGKQNLPHPEDGDGFFYAKIIKK

Specific function: Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=352, Percent_Identity=31.5340909090909, Blast_Score=139, Evalue=7e-33,
Organism=Homo sapiens, GI76150623, Length=352, Percent_Identity=31.5340909090909, Blast_Score=139, Evalue=7e-33,
Organism=Homo sapiens, GI32698918, Length=188, Percent_Identity=34.5744680851064, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI39995082, Length=306, Percent_Identity=26.797385620915, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI11545785, Length=169, Percent_Identity=34.3195266272189, Blast_Score=83, Evalue=5e-16,
Organism=Homo sapiens, GI301336155, Length=280, Percent_Identity=27.1428571428571, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI40316918, Length=163, Percent_Identity=31.9018404907975, Blast_Score=66, Evalue=8e-11,
Organism=Escherichia coli, GI2367212, Length=429, Percent_Identity=73.8927738927739, Blast_Score=658, Evalue=0.0,
Organism=Escherichia coli, GI87081985, Length=312, Percent_Identity=28.8461538461538, Blast_Score=93, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17536757, Length=314, Percent_Identity=29.9363057324841, Blast_Score=128, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI25143471, Length=206, Percent_Identity=34.9514563106796, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI115534021, Length=173, Percent_Identity=31.2138728323699, Blast_Score=73, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71998419, Length=173, Percent_Identity=31.2138728323699, Blast_Score=72, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6324268, Length=342, Percent_Identity=31.2865497076023, Blast_Score=135, Evalue=9e-33,
Organism=Saccharomyces cerevisiae, GI6319447, Length=187, Percent_Identity=29.9465240641711, Blast_Score=70, Evalue=7e-13,
Organism=Drosophila melanogaster, GI22024126, Length=322, Percent_Identity=29.5031055900621, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24668781, Length=250, Percent_Identity=32.4, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI21356513, Length=232, Percent_Identity=33.1896551724138, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21355201, Length=220, Percent_Identity=32.7272727272727, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI19920494, Length=171, Percent_Identity=29.8245614035088, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI21356579, Length=260, Percent_Identity=26.5384615384615, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB [H]

EC number: =2.1.1.176 [H]

Molecular weight: Translated: 48444; Mature: 48444

Theoretical pI: Translated: 7.83; Mature: 7.83

Prosite motif: PS01153 NOL1_NOP2_SUN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNTYNLRSIAAKAISQVLDQGQSLSAVLPELQKNISDKDRALLQELCFGTLRVLPQLEW
CCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
CIQQLMARPMTGKQRVFHYLIMVGLYQLIYTRIPPHAALAETVEGATVLKRPQLKGLING
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHCCHHHHHHHHH
VLRQFQRQQVELLERAVNNDSHYLHPSWLLARIKQAYPAQWQQILDANNQRPPMWLRVNR
HHHHHHHHHHHHHHHHHCCCCCEECHHHHHHHHHHHCHHHHHHHHHCCCCCCCEEEEHHH
LHHSRSEYLELLTQADINAEPHPIYRDAVRLITPCAVNHLPGFELGWVTVQDASAQGCVD
HHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHH
LLDPQNGEQILDLCAAPGGKTTHILEAAPKAHVLAVDIDEQRLSRVKENLQRLQLQAVVR
HCCCCCHHHHHHHHCCCCCCCCHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHH
VGDGRAPDTWCGDQQFDRILLDAPCSATGVIRRHPDIKWLRRDRDISELAQLQSEIIEAI
HCCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHCCCCHHHHHCCCCHHHHHHHHHHHHHHH
WPKLKHGGVLVYATCSILPEENQQQIAAFLQRHPEAQLTETGTTAAPGKQNLPHPEDGDG
HHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCCCCCCC
FFYAKIIKK
EEEHEECCC
>Mature Secondary Structure
MKNTYNLRSIAAKAISQVLDQGQSLSAVLPELQKNISDKDRALLQELCFGTLRVLPQLEW
CCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
CIQQLMARPMTGKQRVFHYLIMVGLYQLIYTRIPPHAALAETVEGATVLKRPQLKGLING
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHCCHHHHHHHHH
VLRQFQRQQVELLERAVNNDSHYLHPSWLLARIKQAYPAQWQQILDANNQRPPMWLRVNR
HHHHHHHHHHHHHHHHHCCCCCEECHHHHHHHHHHHCHHHHHHHHHCCCCCCCEEEEHHH
LHHSRSEYLELLTQADINAEPHPIYRDAVRLITPCAVNHLPGFELGWVTVQDASAQGCVD
HHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHH
LLDPQNGEQILDLCAAPGGKTTHILEAAPKAHVLAVDIDEQRLSRVKENLQRLQLQAVVR
HCCCCCHHHHHHHHCCCCCCCCHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHH
VGDGRAPDTWCGDQQFDRILLDAPCSATGVIRRHPDIKWLRRDRDISELAQLQSEIIEAI
HCCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHCCCCHHHHHCCCCHHHHHHHHHHHHHHH
WPKLKHGGVLVYATCSILPEENQQQIAAFLQRHPEAQLTETGTTAAPGKQNLPHPEDGDG
HHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCCCCCCC
FFYAKIIKK
EEEHEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA