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The map label for this gene is rlmB

Identifier: 51594785

GI number: 51594785

Start: 518924

End: 519664

Strand: Direct

Name: rlmB

Synonym: YPTB0433

Alternate gene names: 51594785

Gene position: 518924-519664 (Clockwise)

Preceding gene: 51594784

Following gene: 51594786

Centisome position: 10.94

GC content: 53.98

Gene sequence:

>741_bases
ATGAGCGAAATTATTTACGGTATTCATGCCGTTAAAGCCCTATTAGAACGTGATCCGCAGCGTTTCCTGGAAGTCTTTAT
TCTGAAAGGCCGCGACGATCGACGTTTGCAACCGCTAATCGCAGAGCTGGAAGCCGCCGGTTTAGTGATTCAGGTGGCTA
GCCGCCAGTGGCTGGATTCACAGGTTGAGGGGGGCGTCCATCAGGGGATCGTGGCGCGGGTACGTGAAGGGCGTCAGTAT
CAGGAAAATGACTTACCTGCACTACTGGAAAGTGTCGAAACACCTTTCCTGCTCGTGTTGGATGGCGTCACCGATCCACA
CAATCTGGGGGCATGTTTGCGTAGCGCAGATGCCGCGGGGGTCCATGCGGTTATCGTGCCACGGGATCGCTCAGCACAGC
TAAATGCGATTGCCAAGAAAGTGGCGAGTGGTGCGGCTGAAAACGTGCCATTGATCAAAGTCACCAATTTGGCACGTACC
CTGCGAGTATTGCAGGAACATAACGTCTGGATTGTGGGAACGGCGGGTGAAGCCGATCACACCCTGTATCAAAGCAAAAT
GACCGGTCCGATGGCATTGGTGATGGGGGCAGAAGGTGAAGGTATGCGCCGCTTAACCCGCGAACACTGCGACGAACTGA
TCAGTATTCCAATGGCCGGGAGTGTTTCCTCCCTCAACGTTTCTGTCGCGACAGGTGTCTGTTTATTTGAAGTCGTGCGC
CAGCGTGGATTGAAAGCCTGA

Upstream 100 bases:

>100_bases
TGGTGGTGTGCTACTTGCCGGGCGGCTTTTGTCGCCCGCATATAATTTAATTATTGGTGGGTAAGTCATATTGTCTGCCA
ACACATGCAAAAAGAGCATC

Downstream 100 bases:

>100_bases
TAGCCTAGTCTTGCGGTCGACGCCCCCTTCGTTGTGGTTTTACTCCGCAGCCTAAAGCCAACATCTTTGTATGTTGGCTC
AGACTGCTGACAAACCCCGA

Product: 23S rRNA (guanosine-2'-O-)-methyltransferase

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase

Number of amino acids: Translated: 246; Mature: 245

Protein sequence:

>246_residues
MSEIIYGIHAVKALLERDPQRFLEVFILKGRDDRRLQPLIAELEAAGLVIQVASRQWLDSQVEGGVHQGIVARVREGRQY
QENDLPALLESVETPFLLVLDGVTDPHNLGACLRSADAAGVHAVIVPRDRSAQLNAIAKKVASGAAENVPLIKVTNLART
LRVLQEHNVWIVGTAGEADHTLYQSKMTGPMALVMGAEGEGMRRLTREHCDELISIPMAGSVSSLNVSVATGVCLFEVVR
QRGLKA

Sequences:

>Translated_246_residues
MSEIIYGIHAVKALLERDPQRFLEVFILKGRDDRRLQPLIAELEAAGLVIQVASRQWLDSQVEGGVHQGIVARVREGRQY
QENDLPALLESVETPFLLVLDGVTDPHNLGACLRSADAAGVHAVIVPRDRSAQLNAIAKKVASGAAENVPLIKVTNLART
LRVLQEHNVWIVGTAGEADHTLYQSKMTGPMALVMGAEGEGMRRLTREHCDELISIPMAGSVSSLNVSVATGVCLFEVVR
QRGLKA
>Mature_245_residues
SEIIYGIHAVKALLERDPQRFLEVFILKGRDDRRLQPLIAELEAAGLVIQVASRQWLDSQVEGGVHQGIVARVREGRQYQ
ENDLPALLESVETPFLLVLDGVTDPHNLGACLRSADAAGVHAVIVPRDRSAQLNAIAKKVASGAAENVPLIKVTNLARTL
RVLQEHNVWIVGTAGEADHTLYQSKMTGPMALVMGAEGEGMRRLTREHCDELISIPMAGSVSSLNVSVATGVCLFEVVRQ
RGLKA

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily

Homologues:

Organism=Homo sapiens, GI40068479, Length=222, Percent_Identity=31.0810810810811, Blast_Score=84, Evalue=9e-17,
Organism=Escherichia coli, GI1790623, Length=242, Percent_Identity=81.8181818181818, Blast_Score=416, Evalue=1e-118,
Organism=Escherichia coli, GI1788935, Length=240, Percent_Identity=28.3333333333333, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1790083, Length=161, Percent_Identity=30.4347826086957, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17555908, Length=259, Percent_Identity=27.027027027027, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324775, Length=165, Percent_Identity=31.5151515151515, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMB_YERPE (Q8ZIV4)

Other databases:

- EMBL:   AL590842
- EMBL:   AE009952
- EMBL:   AE017042
- PIR:   AE0047
- RefSeq:   NP_667975.1
- RefSeq:   NP_991930.1
- RefSeq:   YP_002345459.1
- ProteinModelPortal:   Q8ZIV4
- SMR:   Q8ZIV4
- GeneID:   1145585
- GeneID:   1173227
- GeneID:   2764181
- GenomeReviews:   AE009952_GR
- GenomeReviews:   AE017042_GR
- GenomeReviews:   AL590842_GR
- KEGG:   ype:YPO0381
- KEGG:   ypk:y0638
- KEGG:   ypm:YP_0537
- HOGENOM:   HBG714057
- OMA:   VEGVHQG
- ProtClustDB:   PRK11181
- BioCyc:   YPES187410:Y0638-MONOMER
- BioCyc:   YPES214092:YPO0381-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01887
- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123
- SMART:   SM00967
- TIGRFAMs:   TIGR00186

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26722; Mature: 26591

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: BINDING 196-196 BINDING 216-216 BINDING 225-225

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEIIYGIHAVKALLERDPQRFLEVFILKGRDDRRLQPLIAELEAAGLVIQVASRQWLDS
CCHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCEEEEEHHHHHHHH
QVEGGVHQGIVARVREGRQYQENDLPALLESVETPFLLVLDGVTDPHNLGACLRSADAAG
HHCCCHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCCCCC
VHAVIVPRDRSAQLNAIAKKVASGAAENVPLIKVTNLARTLRVLQEHNVWIVGTAGEADH
EEEEEEECCCCHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHCCEEEEECCCCCHH
TLYQSKMTGPMALVMGAEGEGMRRLTREHCDELISIPMAGSVSSLNVSVATGVCLFEVVR
HHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHHH
QRGLKA
HCCCCC
>Mature Secondary Structure 
SEIIYGIHAVKALLERDPQRFLEVFILKGRDDRRLQPLIAELEAAGLVIQVASRQWLDS
CHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCEEEEEHHHHHHHH
QVEGGVHQGIVARVREGRQYQENDLPALLESVETPFLLVLDGVTDPHNLGACLRSADAAG
HHCCCHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCCCCC
VHAVIVPRDRSAQLNAIAKKVASGAAENVPLIKVTNLARTLRVLQEHNVWIVGTAGEADH
EEEEEEECCCCHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHCCEEEEECCCCCHH
TLYQSKMTGPMALVMGAEGEGMRRLTREHCDELISIPMAGSVSSLNVSVATGVCLFEVVR
HHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHHH
QRGLKA
HCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430