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The map label for this gene is amiB [H]

Identifier: 51594774

GI number: 51594774

Start: 504490

End: 506271

Strand: Direct

Name: amiB [H]

Synonym: YPTB0422

Alternate gene names: 51594774

Gene position: 504490-506271 (Clockwise)

Preceding gene: 51594773

Following gene: 51594775

Centisome position: 10.63

GC content: 50.9

Gene sequence:

>1782_bases
ATGGTTATTGGTTTATTAACGCTCAGTTTATTCGCTCTGCCTCCCGCATTTGCCATGAAACTCACTGACATTAAGGTGAC
CAACGGGGCGAGTGAAAGTAAGGTTACTCTCAGCTTTGATGGTAAGCCAATTTATGCATTTTTCTCGCTAAACTCGCCGG
AGCGGGTGGTCTTGGATGTCCGCCAAAGTGGCAATATCTCAGGGTTGCCGCTGGAGTTCAGTGGGCAGAACCTGTTGAAA
CGAATTCGCTCCAGCACTCCGAAAGATGAACAAAGTACCCGCCTGGTACTGGAACTGACGCAGAAAGTAAAAACCCGTGC
TGTCACCCAACAGTCGGGCAATAACTACACGGTAGTGCTTACCATGACGGCCGTCGCCAGTGCCCCTGTTCGTCAGGCGC
AGGCCTCATTAAATCAGACGAATACCCCGAGTCCTAATGCCGGGCGTGTGGTGCCTCAAGTCACGAGCAAGGATTCAGTA
GCTAAAAATCCGTTTAATAATAAGCCCGTCGTCGTGGTCAGTAGCGAAAACGTGACCACGAATACTGCCCGTCAGATTAA
AACTGTGAGTGCGGCTAACAGTAGCCGTGTTGTGGTGGCCATAGATGCCGGTCATGGTGGTCAGGACCCAGGCGCTATCG
GGCAAAATGGTCTGAAAGAGAAAAATGTCACGATCTCCATTGCCCGCCGGCTTGAGGCACTGCTCAACCGGGATCCGATG
TTTAAACCGGTGCTGACCCGCAATGGTGATTACTTTATCTCGGTCATGGGGCGCTCTGATGTTGCACGTAAACAGGGGGC
TAATGTCTTGATTTCTATTCATGCTGATGCCGCGCCAAATCGCAGTGCGACAGGGGCATCGGTTTGGGTGTTATCAAACC
GCCGGGCTAATAGTGAAATGGGGAACTGGCTGGAGCAACATGAAAAGCAATCAGAACTGCTCGGGGGCGCTGGTGATGTA
CTGGCAAACACGGCTTCAGACCCTTATTTGAGTCAGGCGGTTCTGGATTTACAATTTGGTCACTCCCAGAGAGTGGGTTA
TGACGTCGCCACTAAAGTGCTGCGTGAGCTACAAACGGTGGGTGATATTCATAAACGCAAGCCGGAGCACGCCAGCCTTG
GGGTGTTACGTTCACCGGATATTCCGTCGCTATTAGTGGAAACCGGCTTTATCAGTAATAGTACGGAGGAACGGCTGCTT
GGCAGTAGCGCGTATCAGGAGAAGATTGCTCAGGCCATTTATAAAGGCTTGCGCAGTTATTTCCTCGCGAATCCGTTACA
AGCCGACCCAAAGGTCGAAAGCCGTCCGCTCATTGAAACAGCGGCGGTTGACTCCTCAACACAGCGTTCAGGTATTAGTC
AGCCGGAACCTATTGTGAATAATATAAGCAGTAATGCGCAAAGTGGCCGGTCATCGGCTACTCTGCCTGCAGGTAAGAGC
AAAATCCACAACGTGCAGCGTGGGGAGACGTTATCGGGTATTGCCAGCCAATACGGCGTCAGCATGGCTGTTTTACGACA
AAATAATACGCTGAAAAATGATGTAGTCTGGGTAGGCCAGCGGTTGAAAGTGCCCGCGTCGGGCAGTACGGTAACGGCAG
TCAAGCCTAAAACAGCCGCTGTAGCAAAAAGTAAACCTATTAAGCATCAGGTGAAACGCGGTGATACGCTGTCAGCCATT
GCGGCGAGATACGGTGTATCAATGAGTGAGATTGAGCGGGTGAATAAAATCAAGTCAGGTAATGTTCAGCTTGGGCAAAC
GCTCACTATTCCACAATCGTAA

Upstream 100 bases:

>100_bases
GAAACACGGTTTCGCTACGATTCGCCATTTCACTACGATTTGCCATTTCACTACTGTCCGCCGCGTCATTACTGCTCGTC
GTTTCGCCACGGTCACGCGG

Downstream 100 bases:

>100_bases
GCGCAAGGAATTGTTATGCCGATTCAGATTCTGCCTCCACAGCTTGCCAACCAGATTGCCGCCGGTGAGGTGGTAGAGCG
GCCTGCATCGGTAGTCAAAG

Product: N-acetylmuramoyl-L-alanine amidase-family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 593; Mature: 593

Protein sequence:

>593_residues
MVIGLLTLSLFALPPAFAMKLTDIKVTNGASESKVTLSFDGKPIYAFFSLNSPERVVLDVRQSGNISGLPLEFSGQNLLK
RIRSSTPKDEQSTRLVLELTQKVKTRAVTQQSGNNYTVVLTMTAVASAPVRQAQASLNQTNTPSPNAGRVVPQVTSKDSV
AKNPFNNKPVVVVSSENVTTNTARQIKTVSAANSSRVVVAIDAGHGGQDPGAIGQNGLKEKNVTISIARRLEALLNRDPM
FKPVLTRNGDYFISVMGRSDVARKQGANVLISIHADAAPNRSATGASVWVLSNRRANSEMGNWLEQHEKQSELLGGAGDV
LANTASDPYLSQAVLDLQFGHSQRVGYDVATKVLRELQTVGDIHKRKPEHASLGVLRSPDIPSLLVETGFISNSTEERLL
GSSAYQEKIAQAIYKGLRSYFLANPLQADPKVESRPLIETAAVDSSTQRSGISQPEPIVNNISSNAQSGRSSATLPAGKS
KIHNVQRGETLSGIASQYGVSMAVLRQNNTLKNDVVWVGQRLKVPASGSTVTAVKPKTAAVAKSKPIKHQVKRGDTLSAI
AARYGVSMSEIERVNKIKSGNVQLGQTLTIPQS

Sequences:

>Translated_593_residues
MVIGLLTLSLFALPPAFAMKLTDIKVTNGASESKVTLSFDGKPIYAFFSLNSPERVVLDVRQSGNISGLPLEFSGQNLLK
RIRSSTPKDEQSTRLVLELTQKVKTRAVTQQSGNNYTVVLTMTAVASAPVRQAQASLNQTNTPSPNAGRVVPQVTSKDSV
AKNPFNNKPVVVVSSENVTTNTARQIKTVSAANSSRVVVAIDAGHGGQDPGAIGQNGLKEKNVTISIARRLEALLNRDPM
FKPVLTRNGDYFISVMGRSDVARKQGANVLISIHADAAPNRSATGASVWVLSNRRANSEMGNWLEQHEKQSELLGGAGDV
LANTASDPYLSQAVLDLQFGHSQRVGYDVATKVLRELQTVGDIHKRKPEHASLGVLRSPDIPSLLVETGFISNSTEERLL
GSSAYQEKIAQAIYKGLRSYFLANPLQADPKVESRPLIETAAVDSSTQRSGISQPEPIVNNISSNAQSGRSSATLPAGKS
KIHNVQRGETLSGIASQYGVSMAVLRQNNTLKNDVVWVGQRLKVPASGSTVTAVKPKTAAVAKSKPIKHQVKRGDTLSAI
AARYGVSMSEIERVNKIKSGNVQLGQTLTIPQS
>Mature_593_residues
MVIGLLTLSLFALPPAFAMKLTDIKVTNGASESKVTLSFDGKPIYAFFSLNSPERVVLDVRQSGNISGLPLEFSGQNLLK
RIRSSTPKDEQSTRLVLELTQKVKTRAVTQQSGNNYTVVLTMTAVASAPVRQAQASLNQTNTPSPNAGRVVPQVTSKDSV
AKNPFNNKPVVVVSSENVTTNTARQIKTVSAANSSRVVVAIDAGHGGQDPGAIGQNGLKEKNVTISIARRLEALLNRDPM
FKPVLTRNGDYFISVMGRSDVARKQGANVLISIHADAAPNRSATGASVWVLSNRRANSEMGNWLEQHEKQSELLGGAGDV
LANTASDPYLSQAVLDLQFGHSQRVGYDVATKVLRELQTVGDIHKRKPEHASLGVLRSPDIPSLLVETGFISNSTEERLL
GSSAYQEKIAQAIYKGLRSYFLANPLQADPKVESRPLIETAAVDSSTQRSGISQPEPIVNNISSNAQSGRSSATLPAGKS
KIHNVQRGETLSGIASQYGVSMAVLRQNNTLKNDVVWVGQRLKVPASGSTVTAVKPKTAAVAKSKPIKHQVKRGDTLSAI
AARYGVSMSEIERVNKIKSGNVQLGQTLTIPQS

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division. Can also act as powerful autolysin in the presence of murein synthesis inhibitors [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1790611, Length=430, Percent_Identity=63.0232558139535, Blast_Score=536, Evalue=1e-153,
Organism=Escherichia coli, GI87082163, Length=434, Percent_Identity=30.4147465437788, Blast_Score=181, Evalue=9e-47,
Organism=Escherichia coli, GI1788776, Length=224, Percent_Identity=42.8571428571429, Blast_Score=181, Evalue=1e-46,
Organism=Escherichia coli, GI1786405, Length=111, Percent_Identity=36.9369369369369, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508 [H]

Pfam domain/function: PF01520 Amidase_3 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 63478; Mature: 63478

Theoretical pI: Translated: 10.66; Mature: 10.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVIGLLTLSLFALPPAFAMKLTDIKVTNGASESKVTLSFDGKPIYAFFSLNSPERVVLDV
CEEHHHHHHHHHCCHHHHEEEEEEEEECCCCCCEEEEEECCCEEEEEEECCCCCEEEEEE
RQSGNISGLPLEFSGQNLLKRIRSSTPKDEQSTRLVLELTQKVKTRAVTQQSGNNYTVVL
ECCCCCCCCEEEECCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
TMTAVASAPVRQAQASLNQTNTPSPNAGRVVPQVTSKDSVAKNPFNNKPVVVVSSENVTT
EEEHHHCCHHHHHHHHHCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCEEEEECCCCCC
NTARQIKTVSAANSSRVVVAIDAGHGGQDPGAIGQNGLKEKNVTISIARRLEALLNRDPM
CHHHHHEEEECCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHCCCCC
FKPVLTRNGDYFISVMGRSDVARKQGANVLISIHADAAPNRSATGASVWVLSNRRANSEM
CCCCEECCCCEEEEEECCCHHHHHCCCEEEEEEECCCCCCCCCCCCEEEEEECCCCCCHH
GNWLEQHEKQSELLGGAGDVLANTASDPYLSQAVLDLQFGHSQRVGYDVATKVLRELQTV
HHHHHHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
GDIHKRKPEHASLGVLRSPDIPSLLVETGFISNSTEERLLGSSAYQEKIAQAIYKGLRSY
HHHHHCCCCCCCCCEECCCCCCHHHHHHCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHH
FLANPLQADPKVESRPLIETAAVDSSTQRSGISQPEPIVNNISSNAQSGRSSATLPAGKS
HHCCCCCCCCCCCCCCCCHHHHCCCCHHHCCCCCCCHHHHHHHCCCCCCCCCCCCCCCHH
KIHNVQRGETLSGIASQYGVSMAVLRQNNTLKNDVVWVGQRLKVPASGSTVTAVKPKTAA
HHHHCCCCCHHHHHHHHHCCEEEEEECCCCCCCCEEEECCEEECCCCCCEEEEECCCHHH
VAKSKPIKHQVKRGDTLSAIAARYGVSMSEIERVNKIKSGNVQLGQTLTIPQS
HHCCCCHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHCCCCEEECEEEECCCC
>Mature Secondary Structure
MVIGLLTLSLFALPPAFAMKLTDIKVTNGASESKVTLSFDGKPIYAFFSLNSPERVVLDV
CEEHHHHHHHHHCCHHHHEEEEEEEEECCCCCCEEEEEECCCEEEEEEECCCCCEEEEEE
RQSGNISGLPLEFSGQNLLKRIRSSTPKDEQSTRLVLELTQKVKTRAVTQQSGNNYTVVL
ECCCCCCCCEEEECCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
TMTAVASAPVRQAQASLNQTNTPSPNAGRVVPQVTSKDSVAKNPFNNKPVVVVSSENVTT
EEEHHHCCHHHHHHHHHCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCEEEEECCCCCC
NTARQIKTVSAANSSRVVVAIDAGHGGQDPGAIGQNGLKEKNVTISIARRLEALLNRDPM
CHHHHHEEEECCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHCCCCC
FKPVLTRNGDYFISVMGRSDVARKQGANVLISIHADAAPNRSATGASVWVLSNRRANSEM
CCCCEECCCCEEEEEECCCHHHHHCCCEEEEEEECCCCCCCCCCCCEEEEEECCCCCCHH
GNWLEQHEKQSELLGGAGDVLANTASDPYLSQAVLDLQFGHSQRVGYDVATKVLRELQTV
HHHHHHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
GDIHKRKPEHASLGVLRSPDIPSLLVETGFISNSTEERLLGSSAYQEKIAQAIYKGLRSY
HHHHHCCCCCCCCCEECCCCCCHHHHHHCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHH
FLANPLQADPKVESRPLIETAAVDSSTQRSGISQPEPIVNNISSNAQSGRSSATLPAGKS
HHCCCCCCCCCCCCCCCCHHHHCCCCHHHCCCCCCCHHHHHHHCCCCCCCCCCCCCCCHH
KIHNVQRGETLSGIASQYGVSMAVLRQNNTLKNDVVWVGQRLKVPASGSTVTAVKPKTAA
HHHHCCCCCHHHHHHHHHCCEEEEEECCCCCCCCEEEECCEEECCCCCCEEEEECCCHHH
VAKSKPIKHQVKRGDTLSAIAARYGVSMSEIERVNKIKSGNVQLGQTLTIPQS
HHCCCCHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHCCCCEEECEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7511774; 7610040; 9278503; 1594459 [H]