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The map label for this gene is thiO/thiG [H]

Identifier: 50842004

GI number: 50842004

Start: 573023

End: 574270

Strand: Reverse

Name: thiO/thiG [H]

Synonym: PPA0520

Alternate gene names: 50842004

Gene position: 574270-573023 (Counterclockwise)

Preceding gene: 50842005

Following gene: 50842003

Centisome position: 22.43

GC content: 62.66

Gene sequence:

>1248_bases
GTGGGCACTGCTGCCACACGACGAGAGTTCGCTGGATCCACTGTCGTGGGTCACGTCGATACGAAGAGCCACAATCACGC
CGCCGAGACTTCGACTATTCCACCGTCGGAGCGTGGTCGTCCCTCCTCTGTCGTCATCGCCGGAGCCGGAATTATCGGTC
TGACGACGGCCTGGCGACTGCGTCGTCGCGGGATAGAGGTCACCGTCTTGGATGACGCCCCAGTTTCCGGGGCCAGTTTC
GCTGCTGCCGGGATGCTCGCCCCGGTCTCCGAGGTCATCTGGGATCAGCCGACCCTCTACCCCCTCATGGTGGAATCGGG
CCGGATCTATCGCGACTTCGCTGCCGCCATAGCCCACGACGTTGGCCACGACATCGGGTATCTGGAAAGCTCCACCTTCG
TCTGCGCTGGAGATTCCGCCGACCGCCAGACCCTCAATGAACTGCTCGAACTGCAGCACCGGATGGGCATGACAGTCAAC
CGAATCAGTGTGTCCCAGGCACGGGCGGCGGAACCCGCGCTGGGCCCAGGATGCGTCGGGGTCGTCGACATCCCCGGCGA
CCATCAAGTTGATCCCCGACGGCTGTGCGGCGCCATTCTCGAATTCCTTGGCGACAGAGTCGTACCCACCCGAGCCAGTG
AGGTTCTCTTCAATGAGGATCATCGCGCGATCGGCCTGCGTGGTGCCAACGGCAAAAGCTACCGTGCCGATCACGTCGTA
CTCGCCGCTGGGGTGGACACCGGTGCGATCACCGGCATTCCGCAGGCCCTCACTGATCTACTGCGCCCGGTACGAGGTGA
GGTGTTGCGACTCCGCGTCCCCGAAGCCCTGCGGCCATTGGTAACAAGAACCATCCGCGGCGTCGTTCAGGGAAATGCCG
TTTACATCGTCCCGCGCGCCGACGGCACCATCGTCCTAGGCGCAACAACTCGAGAAGACGACATGGTCGGAGTGCGTGCG
GAGGGTGTTCACCAGTTGCTGCGCGACGCCCACCGTTTGGTACCCGGAATCCTCGACTGCGAAATCTACGAGATGACTGC
CAAGGCCCGTCCCGGTTCCCCCGACGACGTACCCATGGTTGGACGGGTAGGCGATGGGCTCACTGTCTCAATGGGGTACT
TCCGGCACGGCATTCTCCTGGCTGCGATCGGCTCGAGGATTGGCGCAGATATCGTCTGCGGCACCGAAAAGGACAACAAT
CCGGCATTGCTAGCGGCCGTCAATCCATTCCGATTCGGCAACCGGTAA

Upstream 100 bases:

>100_bases
GTTTCCGCGGGAGCCGCACAGAACAGGATGCGGCTGAGAGGGCATATTCGCCGACCGCTTTCACCTGATGCGGGTCATGC
CGCCGGAGGGAGGACATGCT

Downstream 100 bases:

>100_bases
AAAGCCATGATGACAGCGAACAATGGTGAGACGACTGGCCACTACCCCACGACGACGGTCAACGGGGAGCCGTACGATCC
CAGCGGTAATGAGACGATTC

Product: FAD dependent oxidoreductase

Products: NA

Alternate protein names: Probable FAD-dependent glycine oxidase; Thiazole synthase [H]

Number of amino acids: Translated: 415; Mature: 414

Protein sequence:

>415_residues
MGTAATRREFAGSTVVGHVDTKSHNHAAETSTIPPSERGRPSSVVIAGAGIIGLTTAWRLRRRGIEVTVLDDAPVSGASF
AAAGMLAPVSEVIWDQPTLYPLMVESGRIYRDFAAAIAHDVGHDIGYLESSTFVCAGDSADRQTLNELLELQHRMGMTVN
RISVSQARAAEPALGPGCVGVVDIPGDHQVDPRRLCGAILEFLGDRVVPTRASEVLFNEDHRAIGLRGANGKSYRADHVV
LAAGVDTGAITGIPQALTDLLRPVRGEVLRLRVPEALRPLVTRTIRGVVQGNAVYIVPRADGTIVLGATTREDDMVGVRA
EGVHQLLRDAHRLVPGILDCEIYEMTAKARPGSPDDVPMVGRVGDGLTVSMGYFRHGILLAAIGSRIGADIVCGTEKDNN
PALLAAVNPFRFGNR

Sequences:

>Translated_415_residues
MGTAATRREFAGSTVVGHVDTKSHNHAAETSTIPPSERGRPSSVVIAGAGIIGLTTAWRLRRRGIEVTVLDDAPVSGASF
AAAGMLAPVSEVIWDQPTLYPLMVESGRIYRDFAAAIAHDVGHDIGYLESSTFVCAGDSADRQTLNELLELQHRMGMTVN
RISVSQARAAEPALGPGCVGVVDIPGDHQVDPRRLCGAILEFLGDRVVPTRASEVLFNEDHRAIGLRGANGKSYRADHVV
LAAGVDTGAITGIPQALTDLLRPVRGEVLRLRVPEALRPLVTRTIRGVVQGNAVYIVPRADGTIVLGATTREDDMVGVRA
EGVHQLLRDAHRLVPGILDCEIYEMTAKARPGSPDDVPMVGRVGDGLTVSMGYFRHGILLAAIGSRIGADIVCGTEKDNN
PALLAAVNPFRFGNR
>Mature_414_residues
GTAATRREFAGSTVVGHVDTKSHNHAAETSTIPPSERGRPSSVVIAGAGIIGLTTAWRLRRRGIEVTVLDDAPVSGASFA
AAGMLAPVSEVIWDQPTLYPLMVESGRIYRDFAAAIAHDVGHDIGYLESSTFVCAGDSADRQTLNELLELQHRMGMTVNR
ISVSQARAAEPALGPGCVGVVDIPGDHQVDPRRLCGAILEFLGDRVVPTRASEVLFNEDHRAIGLRGANGKSYRADHVVL
AAGVDTGAITGIPQALTDLLRPVRGEVLRLRVPEALRPLVTRTIRGVVQGNAVYIVPRADGTIVLGATTREDDMVGVRAE
GVHQLLRDAHRLVPGILDCEIYEMTAKARPGSPDDVPMVGRVGDGLTVSMGYFRHGILLAAIGSRIGADIVCGTEKDNNP
ALLAAVNPFRFGNR

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the thiG family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006076
- InterPro:   IPR012727
- InterPro:   IPR008867 [H]

Pfam domain/function: PF01266 DAO; PF05690 ThiG [H]

EC number: NA

Molecular weight: Translated: 44109; Mature: 43978

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTAATRREFAGSTVVGHVDTKSHNHAAETSTIPPSERGRPSSVVIAGAGIIGLTTAWRL
CCCCHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHH
RRRGIEVTVLDDAPVSGASFAAAGMLAPVSEVIWDQPTLYPLMVESGRIYRDFAAAIAHD
HHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHH
VGHDIGYLESSTFVCAGDSADRQTLNELLELQHRMGMTVNRISVSQARAAEPALGPGCVG
HCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCEEEEEEHHHHHCCCCCCCCCCEE
VVDIPGDHQVDPRRLCGAILEFLGDRVVPTRASEVLFNEDHRAIGLRGANGKSYRADHVV
EEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHEECCCCCEEEEECCCCCCCCCCEEE
LAAGVDTGAITGIPQALTDLLRPVRGEVLRLRVPEALRPLVTRTIRGVVQGNAVYIVPRA
EEECCCCCCCCCCHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHCCCCEEEEEECC
DGTIVLGATTREDDMVGVRAEGVHQLLRDAHRLVPGILDCEIYEMTAKARPGSPDDVPMV
CCEEEEEECCCCCCEEECCHHHHHHHHHHHHHHCCCHHCCEEEEEECCCCCCCCCCCCEE
GRVGDGLTVSMGYFRHGILLAAIGSRIGADIVCGTEKDNNPALLAAVNPFRFGNR
EECCCCEEEEHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCEEEEEECCCCCCCC
>Mature Secondary Structure 
GTAATRREFAGSTVVGHVDTKSHNHAAETSTIPPSERGRPSSVVIAGAGIIGLTTAWRL
CCCHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHH
RRRGIEVTVLDDAPVSGASFAAAGMLAPVSEVIWDQPTLYPLMVESGRIYRDFAAAIAHD
HHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHH
VGHDIGYLESSTFVCAGDSADRQTLNELLELQHRMGMTVNRISVSQARAAEPALGPGCVG
HCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCEEEEEEHHHHHCCCCCCCCCCEE
VVDIPGDHQVDPRRLCGAILEFLGDRVVPTRASEVLFNEDHRAIGLRGANGKSYRADHVV
EEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHEECCCCCEEEEECCCCCCCCCCEEE
LAAGVDTGAITGIPQALTDLLRPVRGEVLRLRVPEALRPLVTRTIRGVVQGNAVYIVPRA
EEECCCCCCCCCCHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHCCCCEEEEEECC
DGTIVLGATTREDDMVGVRAEGVHQLLRDAHRLVPGILDCEIYEMTAKARPGSPDDVPMV
CCEEEEEECCCCCCEEECCHHHHHHHHHHHHHHCCCHHCCEEEEEECCCCCCCCCCCCEE
GRVGDGLTVSMGYFRHGILLAAIGSRIGADIVCGTEKDNNPALLAAVNPFRFGNR
EECCCCEEEEHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA