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The map label for this gene is thiG

Identifier: 50842003

GI number: 50842003

Start: 571872

End: 572705

Strand: Reverse

Name: thiG

Synonym: PPA0519

Alternate gene names: 50842003

Gene position: 572705-571872 (Counterclockwise)

Preceding gene: 50842004

Following gene: 50842002

Centisome position: 22.37

GC content: 62.71

Gene sequence:

>834_bases
GTGAGCTGTGACGAAAAGGCGGCGACGTCTTCCGACGAACCGCTGCGCATAGGTGGTCTAAGGCTCACATCACGACTCAT
CATGGGCACCGGTGGTAGCACGAGCATGGACACCCTCGAGCGAGCACTGGTTGCCTCGGGGACTCAACTTACGACCGTGG
CTATGCGTCGATTCGCCGCCGGGCCTCGTCAATCGGTGTTCGAGATCCTGCAACGGCACCGGATCACCGCCCTACCGAAC
ACGGCCGGCTGCTACACGGCACGAGACGCTATTCTCACCGCGAATCTGGCCAGGGAAGCCCTGGATACCAACCTCGTCAA
ACTCGAGGTCATCGCTGATGAGGACACCCTGCTGCCCGACCCCGTTGAGCTCGTGAACGCCGCCGAACAACTCGTGGCCA
ATGATTTCGTCGTGTTGGCCTATACCAATGACGATCCAGCAATAGCTAAGCGTCTGGAAGATCTCGGTTGCGCTGCGGTG
ATGCCGGCCGGAGCCCCGATCGGTACCGGGCTAGGAATCCTTAACCCGCACAATATCGAGTTGATTGTCGACCGGGCGAA
CGTCCCCGTCATCCTGGACGCCGGGATCGGCACGGCCTCGGAGGCCACCTTGGCCATGGAACTGGGGTGCGACGCGGTAC
TCCTGGCGTCCGCCGTGACCCGCGCTCACAATCCGGTGGGCATGGCCCAGGCGATGAAGATGGCTGTGGTGGCTGGACGG
ATGGCTCGTACATCGGGACGCATCCCGCGTCGACGTCTGGCGCGAGCCAGCTCGCCGTTCGACGGGATCATCACTGGGCG
AGTCCGTGGTCCGAGGGAGAACGACAGTCTGTGA

Upstream 100 bases:

>100_bases
GGGCGGCAACCCCGGTGCGAGGAGTCATCGACGTCGTGACGGCGGTACAAGGCGGATGATGACGGAAGAAACAGACGTGG
CGAACGTGAAGGAGACGATA

Downstream 100 bases:

>100_bases
GCGCTCTAAACCCGATGGACGACGCGGTATCTCGTGCTACCAACGGCCACGATGCCGTAGAAGACATGGGTTCCACGACG
TCGCTAATCAACCGTGACCT

Product: thiazole synthase

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 277; Mature: 276

Protein sequence:

>277_residues
MSCDEKAATSSDEPLRIGGLRLTSRLIMGTGGSTSMDTLERALVASGTQLTTVAMRRFAAGPRQSVFEILQRHRITALPN
TAGCYTARDAILTANLAREALDTNLVKLEVIADEDTLLPDPVELVNAAEQLVANDFVVLAYTNDDPAIAKRLEDLGCAAV
MPAGAPIGTGLGILNPHNIELIVDRANVPVILDAGIGTASEATLAMELGCDAVLLASAVTRAHNPVGMAQAMKMAVVAGR
MARTSGRIPRRRLARASSPFDGIITGRVRGPRENDSL

Sequences:

>Translated_277_residues
MSCDEKAATSSDEPLRIGGLRLTSRLIMGTGGSTSMDTLERALVASGTQLTTVAMRRFAAGPRQSVFEILQRHRITALPN
TAGCYTARDAILTANLAREALDTNLVKLEVIADEDTLLPDPVELVNAAEQLVANDFVVLAYTNDDPAIAKRLEDLGCAAV
MPAGAPIGTGLGILNPHNIELIVDRANVPVILDAGIGTASEATLAMELGCDAVLLASAVTRAHNPVGMAQAMKMAVVAGR
MARTSGRIPRRRLARASSPFDGIITGRVRGPRENDSL
>Mature_276_residues
SCDEKAATSSDEPLRIGGLRLTSRLIMGTGGSTSMDTLERALVASGTQLTTVAMRRFAAGPRQSVFEILQRHRITALPNT
AGCYTARDAILTANLAREALDTNLVKLEVIADEDTLLPDPVELVNAAEQLVANDFVVLAYTNDDPAIAKRLEDLGCAAVM
PAGAPIGTGLGILNPHNIELIVDRANVPVILDAGIGTASEATLAMELGCDAVLLASAVTRAHNPVGMAQAMKMAVVAGRM
ARTSGRIPRRRLARASSPFDGIITGRVRGPRENDSL

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family

Homologues:

Organism=Escherichia coli, GI48994993, Length=253, Percent_Identity=48.2213438735178, Blast_Score=217, Evalue=7e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIG_PROAC (Q6AAE4)

Other databases:

- EMBL:   AE017283
- RefSeq:   YP_055230.1
- ProteinModelPortal:   Q6AAE4
- SMR:   Q6AAE4
- GeneID:   2932456
- GenomeReviews:   AE017283_GR
- KEGG:   pac:PPA0519
- NMPDR:   fig|267747.1.peg.507
- HOGENOM:   HBG296821
- OMA:   VAIRRTN
- ProtClustDB:   PRK00208
- BioCyc:   PACN267747:PPA0519-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00443
- InterPro:   IPR013785
- InterPro:   IPR008867
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF05690 ThiG; SSF110399 ThiG

EC number: NA

Molecular weight: Translated: 29163; Mature: 29032

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: NA

Important sites: ACT_SITE 107-107 BINDING 168-168

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSCDEKAATSSDEPLRIGGLRLTSRLIMGTGGSTSMDTLERALVASGTQLTTVAMRRFAA
CCCCCCCCCCCCCCEEECCCEEEEEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHCC
GPRQSVFEILQRHRITALPNTAGCYTARDAILTANLAREALDTNLVKLEVIADEDTLLPD
CCHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCC
PVELVNAAEQLVANDFVVLAYTNDDPAIAKRLEDLGCAAVMPAGAPIGTGLGILNPHNIE
HHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCEE
LIVDRANVPVILDAGIGTASEATLAMELGCDAVLLASAVTRAHNPVGMAQAMKMAVVAGR
EEEECCCCCEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
MARTSGRIPRRRLARASSPFDGIITGRVRGPRENDSL
HHHHCCCCCHHHHHHCCCCCCCEEECCCCCCCCCCCC
>Mature Secondary Structure 
SCDEKAATSSDEPLRIGGLRLTSRLIMGTGGSTSMDTLERALVASGTQLTTVAMRRFAA
CCCCCCCCCCCCCEEECCCEEEEEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHCC
GPRQSVFEILQRHRITALPNTAGCYTARDAILTANLAREALDTNLVKLEVIADEDTLLPD
CCHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCC
PVELVNAAEQLVANDFVVLAYTNDDPAIAKRLEDLGCAAVMPAGAPIGTGLGILNPHNIE
HHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCEE
LIVDRANVPVILDAGIGTASEATLAMELGCDAVLLASAVTRAHNPVGMAQAMKMAVVAGR
EEEECCCCCEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
MARTSGRIPRRRLARASSPFDGIITGRVRGPRENDSL
HHHHCCCCCHHHHHHCCCCCCCEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA