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The map label for this gene is mscL [H]

Identifier: 50841974

GI number: 50841974

Start: 541896

End: 542264

Strand: Reverse

Name: mscL [H]

Synonym: PPA0491

Alternate gene names: 50841974

Gene position: 542264-541896 (Counterclockwise)

Preceding gene: 50841975

Following gene: 50841966

Centisome position: 21.18

GC content: 55.28

Gene sequence:

>369_bases
ATGAAGGGATTCAAAGATTTTCTGATGCGCGGTAATCTCATTGAACTAGCCGTCGCATTCATCCTTGGCGGCGCATTTTC
CACCGTCGTCAAATCGTTTACGACAATCATCATGGACTTGATCGGCAAGCTGGGCGGCACCCCGAACTTCTCTGGCTGGG
TTCCAGGCGGGGTCCACGTCGGTGCCTTCATCACCGACGTCATCTCCTTCTTCGTCATGTCCTTCGTCATTTATTTCGGG
CTCATTAAGCCCATCGAGCTGGCTACGGAGAAGCTCAAGCGCACCAAGGACGAACCAGTGGCTGCCCCCACCACCGCCGA
CCTACTCATCGAGATTCGCGACGAACTGCGTTCCCGTCCGGTCAAGTGA

Upstream 100 bases:

>100_bases
ACCAGACACAGCGAAGAGTATTAGCGACGCCTTCCCAGTACCGTTACGGTTTTAGCAGGCCCGACGTTTGCGGGCAGCAC
ACCAACCCGAAGGGAGCCCT

Downstream 100 bases:

>100_bases
GGACACGCCAGAGCTGCCAGACTCAGTGCAATAAGCTGTGCGGTGGACGCTCGGCTAACAGACGAGCTTCGTCTGGCCCA
ATGACCACCGCGCCCCGGTG

Product: large conductance mechanosensitive channel

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 122; Mature: 122

Protein sequence:

>122_residues
MKGFKDFLMRGNLIELAVAFILGGAFSTVVKSFTTIIMDLIGKLGGTPNFSGWVPGGVHVGAFITDVISFFVMSFVIYFG
LIKPIELATEKLKRTKDEPVAAPTTADLLIEIRDELRSRPVK

Sequences:

>Translated_122_residues
MKGFKDFLMRGNLIELAVAFILGGAFSTVVKSFTTIIMDLIGKLGGTPNFSGWVPGGVHVGAFITDVISFFVMSFVIYFG
LIKPIELATEKLKRTKDEPVAAPTTADLLIEIRDELRSRPVK
>Mature_122_residues
MKGFKDFLMRGNLIELAVAFILGGAFSTVVKSFTTIIMDLIGKLGGTPNFSGWVPGGVHVGAFITDVISFFVMSFVIYFG
LIKPIELATEKLKRTKDEPVAAPTTADLLIEIRDELRSRPVK

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell [H]

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family [H]

Homologues:

Organism=Escherichia coli, GI1789686, Length=134, Percent_Identity=39.5522388059701, Blast_Score=74, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019823
- InterPro:   IPR001185 [H]

Pfam domain/function: PF01741 MscL [H]

EC number: NA

Molecular weight: Translated: 13351; Mature: 13351

Theoretical pI: Translated: 9.76; Mature: 9.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGFKDFLMRGNLIELAVAFILGGAFSTVVKSFTTIIMDLIGKLGGTPNFSGWVPGGVHV
CCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHH
GAFITDVISFFVMSFVIYFGLIKPIELATEKLKRTKDEPVAAPTTADLLIEIRDELRSRP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCC
VK
CC
>Mature Secondary Structure
MKGFKDFLMRGNLIELAVAFILGGAFSTVVKSFTTIIMDLIGKLGGTPNFSGWVPGGVHV
CCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHH
GAFITDVISFFVMSFVIYFGLIKPIELATEKLKRTKDEPVAAPTTADLLIEIRDELRSRP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCC
VK
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA