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The map label for this gene is radA [H]

Identifier: 50841799

GI number: 50841799

Start: 363162

End: 364559

Strand: Reverse

Name: radA [H]

Synonym: PPA0312

Alternate gene names: 50841799

Gene position: 364559-363162 (Counterclockwise)

Preceding gene: 50841800

Following gene: 229259642

Centisome position: 14.24

GC content: 61.73

Gene sequence:

>1398_bases
ATGGCGAGGAAGACGCAAACTCCGACGTGGGTATGCACCGAGTGCGGCTGGACGACGACCAAATGGGTTGGCCGATGCGG
CGAGTGTCAAACCTGGGGTTCGGTAGTTGAAAAGGGGGCACCGAAGCTCACTGAGGTCACCTCATCGACGCCCACGTCGA
AGGCTGTGCCCATCGGTCAAGTCAGTGAACAAGCCGCTAATCGTCATCTCACCGGGATCAGCGAACTCGACCGGGTACTG
GGTGGTGGTCTAGTGCCTGGAGTCGTGGTTTTGCTCGCCGGAGAGCCAGGAGTCGGAAAATCGACCCTGTTGCTCGACGT
TGCGGCCAAATGGGCTAAAGCGGGCAGGCGCACCCTTTACGTCACTGGGGAGGAGTCGGCTGCCCAGGTAAGGCTGCGGG
CTGGTCGCACGAAGTCGCTAGCCGACGAACTCTACCTAGCTTCTGAGACCGATCTGGGTACGGTGCTTGGACATATCGAG
CAAACCGAGCCCTCCCTCATGGTGTTGGACTCCGTACAAACGGTTGGCACCAGCCAGGCTGACGGCTCCCCCGGCGGCGT
TTCACAGGTTAGGGAGGTCACCGGGGCCCTGGTGCGGGTGGCGAAGAGACGCGGGATGGCAGTCATCATCGTCGGCCATG
TCACCAAAGAAGGGTCGATCGCTGGCCCGCGCACCATGGAACATCTCGTTGACGTCGTCCTCAACTTCGAGGGTGACCGC
CATTCCGGATTCCGTATGGTCCGCGCCACGAAAAACCGCTACGGCCCAGCCGACGAGGTGGGCTGCTTCGAGATGACCGA
CGCCGGAATCATGGAGGTTCCGGACCCGTCTGGCCTATTCACCTCAGACAACGCCGATCCTCAGCCGGGCACCTGTGTGA
CGGTGACGATAGAGGGACGCCGTCCCCTACTCTCAGAGGTGCAGGCGTTGGTAGCTCCGTCAGCTTCCGAGAAATACCCG
CGTCGCATCACCCATGGGGTCGAGGGCGGTCGGGTGGCCATGATTCTGGCCGTGCTGGAACGTAAAGCAGGTTTGCCGTT
GAGCAACCGAGACGTCTACGTCTCGACGGTGGGTGGCGCCCGGGTGTCCGACCCGTCAGCTGACCTAGCGATCGCGGTCT
CGGTCGCCTCCGCAGTTCTTGATACCAATTTCCCGACCCGAGTCGTTGCTCTGGGAGAAGTCGGTCTGGCGGGAGATTTG
CGCCGCGTTCCAGGACTAGAGAGACGAGTGGGTGAGGCGGCACGGTTGGGGTTCGACCTGGCAGTGGTGCCACGCAACTC
TCGGGAGGCACATACCAAGATCCCGACGATGCACGGGTTGCACGTCATCGAAGTCGGGAGTGTGGCCGAAGCTCTTTCGG
TGCTTAACCTGCGCCGACAGCGGAAAGTAAACCAGTGA

Upstream 100 bases:

>100_bases
CCATGTGGTGGCCGCATTACCCGCCGGTGGGCTGACCGGGACACAGCGTGGAACACCGTCGTAGTCGAACCTGTCGGTTC
CTGCTGGCAGGGTAAACGCC

Downstream 100 bases:

>100_bases
TAGTAAGGACATCTGAGGTGAGAACCGCGGCACAGCGAACTAGAGTCGACTCATGAACGACGTCCAGCCCGATCCCGAGA
CGTCCCGGGAGATGCAGGCG

Product: DNA repair protein RadA

Products: NA

Alternate protein names: DNA repair protein sms homolog [H]

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MARKTQTPTWVCTECGWTTTKWVGRCGECQTWGSVVEKGAPKLTEVTSSTPTSKAVPIGQVSEQAANRHLTGISELDRVL
GGGLVPGVVVLLAGEPGVGKSTLLLDVAAKWAKAGRRTLYVTGEESAAQVRLRAGRTKSLADELYLASETDLGTVLGHIE
QTEPSLMVLDSVQTVGTSQADGSPGGVSQVREVTGALVRVAKRRGMAVIIVGHVTKEGSIAGPRTMEHLVDVVLNFEGDR
HSGFRMVRATKNRYGPADEVGCFEMTDAGIMEVPDPSGLFTSDNADPQPGTCVTVTIEGRRPLLSEVQALVAPSASEKYP
RRITHGVEGGRVAMILAVLERKAGLPLSNRDVYVSTVGGARVSDPSADLAIAVSVASAVLDTNFPTRVVALGEVGLAGDL
RRVPGLERRVGEAARLGFDLAVVPRNSREAHTKIPTMHGLHVIEVGSVAEALSVLNLRRQRKVNQ

Sequences:

>Translated_465_residues
MARKTQTPTWVCTECGWTTTKWVGRCGECQTWGSVVEKGAPKLTEVTSSTPTSKAVPIGQVSEQAANRHLTGISELDRVL
GGGLVPGVVVLLAGEPGVGKSTLLLDVAAKWAKAGRRTLYVTGEESAAQVRLRAGRTKSLADELYLASETDLGTVLGHIE
QTEPSLMVLDSVQTVGTSQADGSPGGVSQVREVTGALVRVAKRRGMAVIIVGHVTKEGSIAGPRTMEHLVDVVLNFEGDR
HSGFRMVRATKNRYGPADEVGCFEMTDAGIMEVPDPSGLFTSDNADPQPGTCVTVTIEGRRPLLSEVQALVAPSASEKYP
RRITHGVEGGRVAMILAVLERKAGLPLSNRDVYVSTVGGARVSDPSADLAIAVSVASAVLDTNFPTRVVALGEVGLAGDL
RRVPGLERRVGEAARLGFDLAVVPRNSREAHTKIPTMHGLHVIEVGSVAEALSVLNLRRQRKVNQ
>Mature_464_residues
ARKTQTPTWVCTECGWTTTKWVGRCGECQTWGSVVEKGAPKLTEVTSSTPTSKAVPIGQVSEQAANRHLTGISELDRVLG
GGLVPGVVVLLAGEPGVGKSTLLLDVAAKWAKAGRRTLYVTGEESAAQVRLRAGRTKSLADELYLASETDLGTVLGHIEQ
TEPSLMVLDSVQTVGTSQADGSPGGVSQVREVTGALVRVAKRRGMAVIIVGHVTKEGSIAGPRTMEHLVDVVLNFEGDRH
SGFRMVRATKNRYGPADEVGCFEMTDAGIMEVPDPSGLFTSDNADPQPGTCVTVTIEGRRPLLSEVQALVAPSASEKYPR
RITHGVEGGRVAMILAVLERKAGLPLSNRDVYVSTVGGARVSDPSADLAIAVSVASAVLDTNFPTRVVALGEVGLAGDLR
RVPGLERRVGEAARLGFDLAVVPRNSREAHTKIPTMHGLHVIEVGSVAEALSVLNLRRQRKVNQ

Specific function: May play a role in the repair of endogenous alkylation damage [H]

COG id: COG1066

COG function: function code O; Predicted ATP-dependent serine protease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family. RadA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790850, Length=456, Percent_Identity=42.3245614035088, Blast_Score=359, Evalue=1e-100,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR014774
- InterPro:   IPR004504
- InterPro:   IPR008269
- InterPro:   IPR020568 [H]

Pfam domain/function: PF06745 KaiC; PF05362 Lon_C [H]

EC number: NA

Molecular weight: Translated: 49220; Mature: 49089

Theoretical pI: Translated: 8.38; Mature: 8.38

Prosite motif: PS50162 RECA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARKTQTPTWVCTECGWTTTKWVGRCGECQTWGSVVEKGAPKLTEVTSSTPTSKAVPIGQ
CCCCCCCCCEEEECCCCCHHHHHCCCCCCHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCH
VSEQAANRHLTGISELDRVLGGGLVPGVVVLLAGEPGVGKSTLLLDVAAKWAKAGRRTLY
HHHHHHHCCCCCHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEE
VTGEESAAQVRLRAGRTKSLADELYLASETDLGTVLGHIEQTEPSLMVLDSVQTVGTSQA
EECCCCCCEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEEECHHHCCCCCC
DGSPGGVSQVREVTGALVRVAKRRGMAVIIVGHVTKEGSIAGPRTMEHLVDVVLNFEGDR
CCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCC
HSGFRMVRATKNRYGPADEVGCFEMTDAGIMEVPDPSGLFTSDNADPQPGTCVTVTIEGR
CCCEEEEEEHHCCCCCCCCCCCEEECCCCCEECCCCCCCCCCCCCCCCCCCEEEEEECCC
RPLLSEVQALVAPSASEKYPRRITHGVEGGRVAMILAVLERKAGLPLSNRDVYVSTVGGA
CCHHHHHHHHHCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCC
RVSDPSADLAIAVSVASAVLDTNFPTRVVALGEVGLAGDLRRVPGLERRVGEAARLGFDL
CCCCCCCCEEEHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHCCCHHHHHHHHHHCCCEE
AVVPRNSREAHTKIPTMHGLHVIEVGSVAEALSVLNLRRQRKVNQ
EEECCCCCCHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ARKTQTPTWVCTECGWTTTKWVGRCGECQTWGSVVEKGAPKLTEVTSSTPTSKAVPIGQ
CCCCCCCCEEEECCCCCHHHHHCCCCCCHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCH
VSEQAANRHLTGISELDRVLGGGLVPGVVVLLAGEPGVGKSTLLLDVAAKWAKAGRRTLY
HHHHHHHCCCCCHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEE
VTGEESAAQVRLRAGRTKSLADELYLASETDLGTVLGHIEQTEPSLMVLDSVQTVGTSQA
EECCCCCCEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEEECHHHCCCCCC
DGSPGGVSQVREVTGALVRVAKRRGMAVIIVGHVTKEGSIAGPRTMEHLVDVVLNFEGDR
CCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCC
HSGFRMVRATKNRYGPADEVGCFEMTDAGIMEVPDPSGLFTSDNADPQPGTCVTVTIEGR
CCCEEEEEEHHCCCCCCCCCCCEEECCCCCEECCCCCCCCCCCCCCCCCCCEEEEEECCC
RPLLSEVQALVAPSASEKYPRRITHGVEGGRVAMILAVLERKAGLPLSNRDVYVSTVGGA
CCHHHHHHHHHCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCC
RVSDPSADLAIAVSVASAVLDTNFPTRVVALGEVGLAGDLRRVPGLERRVGEAARLGFDL
CCCCCCCCEEEHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHCCCHHHHHHHHHHCCCEE
AVVPRNSREAHTKIPTMHGLHVIEVGSVAEALSVLNLRRQRKVNQ
EEECCCCCCHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]