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The map label for this gene is hemB [H]

Identifier: 50841790

GI number: 50841790

Start: 352402

End: 353448

Strand: Reverse

Name: hemB [H]

Synonym: PPA0302

Alternate gene names: 50841790

Gene position: 353448-352402 (Counterclockwise)

Preceding gene: 50841791

Following gene: 50841789

Centisome position: 13.81

GC content: 61.89

Gene sequence:

>1047_bases
ATGAACCCGTACCTGCCTGATGCCAGTGATCCCCGCATGGCTGGGGTCAAGCCATTGCCCGAGGGGCTGCGTCCTGTTTC
TCGTCCGCGTCGACTACGCACCACTCCTGCGATGCGCCGGATGGTCGCCGAAACCCGCGTCGCCCCCGCCCAGTTAATGC
TGCCGGCGTTCGTCAAAGAGGGGATCGATTCCCCCGTCGAGATCACCAGCCTGCCCGGCCAATTCCAGCACTCCACCGAG
TCGATCAAGGAGCTCGCCACCCAAGCTGCCGAGGCCGGTATTGGTGGTATTGACCTGTTTGGTATCCCGGAGCACAAAGA
CGAGATCGGTTCTCAGGCCTGGGACCTGAAAGGCATCCTCAACGCCGGCATCTCCGCGGTGCGCGAGGCCGTCGGCGACG
ATCTGGTGATCTGTGCCGACACCTGTCTAGACGAGTTCACCGATCATGGACACTGCGGAGCTCTGACCCCTGATGGTCGC
GTCGATAATGACTCCACTGTGCCGCTATATGCCGCGATGGCGGTCTCCCAAGCGCGCGCCGGAGCCCACATGGTCTCCCC
GTCGGGGATGATGGACGGTCAGATCGCTGTCATTCGCGATACTCTGGATCGCGAGGGATTCACCGATGTGTCCATCATGG
CCTATTCAGCTAAGTACGCTTCAGCCTTCTTTGGCCCCTTCCGCGACGCCGTCAACTGTTCCCTTAAGGGTGATCGCAAG
ACTTACCAGCAGGATCCACCGAACCGCCGGGAAGGCCTGCGCGAAACCCTTCTCGACCTCGCCGAGGGAGCCGACCTGGT
CATGGTGAAGCCCGCCTCCCACTACCTCGACGTGTTGTCCGATGTCGCCGAGGTGTCGCAGGTACCGGTTGCCGCTTACC
AGGTCTCTGGAGAATACGCCATGCTCGAGGCCGCTGCCGCAAACGGTTGGATCGATCGTCGACGTTGCATCGGAGAGTCC
CTGACCTCGATCGTGCGTGCCGGTGCCGATCTTGTCTTGACCTACTGGGCAATCGAGGCGGCGCAGATGTTTCGCGAGGA
CCTCTGA

Upstream 100 bases:

>100_bases
CAGCGCATGCGGTGGCCTCCTCCCCCACTCCTGAAGCCGTCCTTGAGGCGGTTGAGTCCTTGGTCGCCTGACCGACGGCC
GTTCGCGAAGGAAGTTGATC

Downstream 100 bases:

>100_bases
CCCGTCGTGATCATGCTCACCACAAACCCCCACACCGGAAGGAAGAAATGACCTCCAACGCCGAACTGTTCTCCGCCGCC
CAGGCCGTCATTCCCGGCGG

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase [H]

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MNPYLPDASDPRMAGVKPLPEGLRPVSRPRRLRTTPAMRRMVAETRVAPAQLMLPAFVKEGIDSPVEITSLPGQFQHSTE
SIKELATQAAEAGIGGIDLFGIPEHKDEIGSQAWDLKGILNAGISAVREAVGDDLVICADTCLDEFTDHGHCGALTPDGR
VDNDSTVPLYAAMAVSQARAGAHMVSPSGMMDGQIAVIRDTLDREGFTDVSIMAYSAKYASAFFGPFRDAVNCSLKGDRK
TYQQDPPNRREGLRETLLDLAEGADLVMVKPASHYLDVLSDVAEVSQVPVAAYQVSGEYAMLEAAAANGWIDRRRCIGES
LTSIVRAGADLVLTYWAIEAAQMFREDL

Sequences:

>Translated_348_residues
MNPYLPDASDPRMAGVKPLPEGLRPVSRPRRLRTTPAMRRMVAETRVAPAQLMLPAFVKEGIDSPVEITSLPGQFQHSTE
SIKELATQAAEAGIGGIDLFGIPEHKDEIGSQAWDLKGILNAGISAVREAVGDDLVICADTCLDEFTDHGHCGALTPDGR
VDNDSTVPLYAAMAVSQARAGAHMVSPSGMMDGQIAVIRDTLDREGFTDVSIMAYSAKYASAFFGPFRDAVNCSLKGDRK
TYQQDPPNRREGLRETLLDLAEGADLVMVKPASHYLDVLSDVAEVSQVPVAAYQVSGEYAMLEAAAANGWIDRRRCIGES
LTSIVRAGADLVLTYWAIEAAQMFREDL
>Mature_348_residues
MNPYLPDASDPRMAGVKPLPEGLRPVSRPRRLRTTPAMRRMVAETRVAPAQLMLPAFVKEGIDSPVEITSLPGQFQHSTE
SIKELATQAAEAGIGGIDLFGIPEHKDEIGSQAWDLKGILNAGISAVREAVGDDLVICADTCLDEFTDHGHCGALTPDGR
VDNDSTVPLYAAMAVSQARAGAHMVSPSGMMDGQIAVIRDTLDREGFTDVSIMAYSAKYASAFFGPFRDAVNCSLKGDRK
TYQQDPPNRREGLRETLLDLAEGADLVMVKPASHYLDVLSDVAEVSQVPVAAYQVSGEYAMLEAAAANGWIDRRRCIGES
LTSIVRAGADLVLTYWAIEAAQMFREDL

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen [H]

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family [H]

Homologues:

Organism=Homo sapiens, GI189083849, Length=310, Percent_Identity=38.3870967741935, Blast_Score=204, Evalue=1e-52,
Organism=Escherichia coli, GI87081728, Length=317, Percent_Identity=47.0031545741325, Blast_Score=290, Evalue=1e-79,
Organism=Saccharomyces cerevisiae, GI6321398, Length=319, Percent_Identity=38.871473354232, Blast_Score=213, Evalue=3e-56,
Organism=Drosophila melanogaster, GI21358291, Length=294, Percent_Identity=38.0952380952381, Blast_Score=193, Evalue=2e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001731
- InterPro:   IPR013785 [H]

Pfam domain/function: PF00490 ALAD [H]

EC number: =4.2.1.24 [H]

Molecular weight: Translated: 37567; Mature: 37567

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPYLPDASDPRMAGVKPLPEGLRPVSRPRRLRTTPAMRRMVAETRVAPAQLMLPAFVKE
CCCCCCCCCCCCCCCCCCCCHHCCCHHCCHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHH
GIDSPVEITSLPGQFQHSTESIKELATQAAEAGIGGIDLFGIPEHKDEIGSQAWDLKGIL
CCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHCCHHHHHHHHH
NAGISAVREAVGDDLVICADTCLDEFTDHGHCGALTPDGRVDNDSTVPLYAAMAVSQARA
HHHHHHHHHHHCCCEEEEHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHC
GAHMVSPSGMMDGQIAVIRDTLDREGFTDVSIMAYSAKYASAFFGPFRDAVNCSLKGDRK
CCEEECCCCCCCCCEEEEEHHHCCCCCCCHHHEEHHHHHHHHHHHHHHHHHCCCCCCCCH
TYQQDPPNRREGLRETLLDLAEGADLVMVKPASHYLDVLSDVAEVSQVPVAAYQVSGEYA
HHCCCCCHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHCCCEEEEECCCHH
MLEAAAANGWIDRRRCIGESLTSIVRAGADLVLTYWAIEAAQMFREDL
HHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNPYLPDASDPRMAGVKPLPEGLRPVSRPRRLRTTPAMRRMVAETRVAPAQLMLPAFVKE
CCCCCCCCCCCCCCCCCCCCHHCCCHHCCHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHH
GIDSPVEITSLPGQFQHSTESIKELATQAAEAGIGGIDLFGIPEHKDEIGSQAWDLKGIL
CCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHCCHHHHHHHHH
NAGISAVREAVGDDLVICADTCLDEFTDHGHCGALTPDGRVDNDSTVPLYAAMAVSQARA
HHHHHHHHHHHCCCEEEEHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHC
GAHMVSPSGMMDGQIAVIRDTLDREGFTDVSIMAYSAKYASAFFGPFRDAVNCSLKGDRK
CCEEECCCCCCCCCEEEEEHHHCCCCCCCHHHEEHHHHHHHHHHHHHHHHHCCCCCCCCH
TYQQDPPNRREGLRETLLDLAEGADLVMVKPASHYLDVLSDVAEVSQVPVAAYQVSGEYA
HHCCCCCHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHCCCEEEEECCCHH
MLEAAAANGWIDRRRCIGESLTSIVRAGADLVLTYWAIEAAQMFREDL
HHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12000953 [H]