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The map label for this gene is emrB [C]

Identifier: 50841774

GI number: 50841774

Start: 334570

End: 336057

Strand: Direct

Name: emrB [C]

Synonym: PPA0285

Alternate gene names: 50841774

Gene position: 334570-336057 (Clockwise)

Preceding gene: 50841773

Following gene: 50841775

Centisome position: 13.07

GC content: 58.67

Gene sequence:

>1488_bases
TTGTCGCCTGAGCAGTCGTCCGTTGCCCATGTCGAACACCTTGCCCGGGACAGAGACGGGCACGTCGTCACTGAGGAGCA
GGCCTGGAGCGCCCTGTGGGCCCTTCTTATCGGCTTCTTTATGATCCTCGTAGATACGTCGATCGTCACGACCGCTCTGC
CGGCGACCATCCGCGCGCTCAACGCCAACCTCAACCAAGGCGTGTGGATCACGAGTGCTTACCTACTCACTTATGCCGTC
CCGCTGCTGATTACCGGACGTATGGGTGATCGCTGGGGACCGCATCGTATGTACATCATCGGCATGCTCGTCTTCGGAGT
TTCTTCACTGGCATGTGGTCTGTCCCCGACGATTGGCGTCCTCATCGCTGCTCGGGCGGTTCAGGGCATCGGAGCTGCCC
TTATGACCCCACAGTCTCTGTCGATGATTACCCGACTTTTCCCACCGGAGAAGCGCGGTGCTGCCATGGGCCTGTGGGGA
GCCACTGCCGGTGTCGCGAGTTTCGTCGGTCCTGTGCTCGGCGGCATCCTTGTTGACACCCTGGGCTGGGAGTGGATCTT
CTTCGTCAACGTCCCGGTTACCGTCATCGGGTTGTGGCTGGCTATCCGTAATGTGCCTGCTCTCGAAACCCACGGCCATG
CCTTCGATTGGTGTGGCGTCGTCTTGTCGGGTATCGGCATGACCCTGCTGGTTTTCGGTATTCAGGAGGGAGAACAGTAC
AACTGGGGGACGATCACCGGCATCATTTCCGTGCCGCTGCTCATCGTCCTCGGTGTGGTCTTTATGGTGCTCTTCGTCGT
CTCACAGACGCAGTGGGCGCAATCCCGCTTGCATGCTCGTTCGACCAACTTTGAACCACTGGTTCCGCTGCGACTGTTTA
CCAAGCGCAATTTCACTCTCGCCAATATCGCGATTTTCCTCGTCGGCATGCTTGTAACTTCCTACTTTCTGCCGACGGCC
ATCTATCTGCAGAACGTGCGTGGCAAGTCCCCGACGGTGGCCGCCCTGTTGTTGATTCCAACCGCTGTCGTCTCCGGCGT
GCTGTCTCCGGTTGTTGGACGGGTCCTCCAGCACCGTCGGTCTGGCCGGATGGCTGCGATTGGCGTCTCGCTATATGTGA
TTTCCTCCATCGGATGGTGGCTGTTCACAGTGCCGTCCACCGGTCTGTGGGTGTTCCCGCTGCTATCTGTCATCACCGGC
GTTGGTGCGGCTCTTACCTGGAGTCCGATTTCCCTGGTTGGTACCGATGACCTCGGTCAGGCCGACGCTGGAGCCGGTTC
AGGTGTTTATAACGCCACCCGTCAGGTCGGGTCAGCGCTGGGATCGGCTTTTATCGCGATGATGATGGATGCCCGAATCA
TGGCTCGCCACGATCTGGGGCGAGGTCTCGGTGAGTCGATGTTTATCACGATTACTGCTGGAACGATCTGCGTAATCGTC
TGTGCATTCTTTGCGAACTTTCAGCGTTCGCGCCAGCGCAATTCTTGA

Upstream 100 bases:

>100_bases
AATCGTGCAGGTTTCAGTGACGATGCGGTACGTGTCCCCATCGACCTGCGGGAGGTACGATCGTCTCTTGGCCTAACTTC
TTCTTTCCCTAGGAGACCCC

Downstream 100 bases:

>100_bases
GCGTACTCATCTGTGAGGCCGGTGGACCGCGCAGGGACGCCCTTCGCTGGACCCGTAGGATGACCATATGAGGATTGCCA
TCGGAGGGTTCGCCGGTCTT

Product: putative sugar transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 495; Mature: 494

Protein sequence:

>495_residues
MSPEQSSVAHVEHLARDRDGHVVTEEQAWSALWALLIGFFMILVDTSIVTTALPATIRALNANLNQGVWITSAYLLTYAV
PLLITGRMGDRWGPHRMYIIGMLVFGVSSLACGLSPTIGVLIAARAVQGIGAALMTPQSLSMITRLFPPEKRGAAMGLWG
ATAGVASFVGPVLGGILVDTLGWEWIFFVNVPVTVIGLWLAIRNVPALETHGHAFDWCGVVLSGIGMTLLVFGIQEGEQY
NWGTITGIISVPLLIVLGVVFMVLFVVSQTQWAQSRLHARSTNFEPLVPLRLFTKRNFTLANIAIFLVGMLVTSYFLPTA
IYLQNVRGKSPTVAALLLIPTAVVSGVLSPVVGRVLQHRRSGRMAAIGVSLYVISSIGWWLFTVPSTGLWVFPLLSVITG
VGAALTWSPISLVGTDDLGQADAGAGSGVYNATRQVGSALGSAFIAMMMDARIMARHDLGRGLGESMFITITAGTICVIV
CAFFANFQRSRQRNS

Sequences:

>Translated_495_residues
MSPEQSSVAHVEHLARDRDGHVVTEEQAWSALWALLIGFFMILVDTSIVTTALPATIRALNANLNQGVWITSAYLLTYAV
PLLITGRMGDRWGPHRMYIIGMLVFGVSSLACGLSPTIGVLIAARAVQGIGAALMTPQSLSMITRLFPPEKRGAAMGLWG
ATAGVASFVGPVLGGILVDTLGWEWIFFVNVPVTVIGLWLAIRNVPALETHGHAFDWCGVVLSGIGMTLLVFGIQEGEQY
NWGTITGIISVPLLIVLGVVFMVLFVVSQTQWAQSRLHARSTNFEPLVPLRLFTKRNFTLANIAIFLVGMLVTSYFLPTA
IYLQNVRGKSPTVAALLLIPTAVVSGVLSPVVGRVLQHRRSGRMAAIGVSLYVISSIGWWLFTVPSTGLWVFPLLSVITG
VGAALTWSPISLVGTDDLGQADAGAGSGVYNATRQVGSALGSAFIAMMMDARIMARHDLGRGLGESMFITITAGTICVIV
CAFFANFQRSRQRNS
>Mature_494_residues
SPEQSSVAHVEHLARDRDGHVVTEEQAWSALWALLIGFFMILVDTSIVTTALPATIRALNANLNQGVWITSAYLLTYAVP
LLITGRMGDRWGPHRMYIIGMLVFGVSSLACGLSPTIGVLIAARAVQGIGAALMTPQSLSMITRLFPPEKRGAAMGLWGA
TAGVASFVGPVLGGILVDTLGWEWIFFVNVPVTVIGLWLAIRNVPALETHGHAFDWCGVVLSGIGMTLLVFGIQEGEQYN
WGTITGIISVPLLIVLGVVFMVLFVVSQTQWAQSRLHARSTNFEPLVPLRLFTKRNFTLANIAIFLVGMLVTSYFLPTAI
YLQNVRGKSPTVAALLLIPTAVVSGVLSPVVGRVLQHRRSGRMAAIGVSLYVISSIGWWLFTVPSTGLWVFPLLSVITGV
GAALTWSPISLVGTDDLGQADAGAGSGVYNATRQVGSALGSAFIAMMMDARIMARHDLGRGLGESMFITITAGTICVIVC
AFFANFQRSRQRNS

Specific function: Translocase That Confers Resistance To Substances Of High Hydrophobicity. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=427, Percent_Identity=28.1030444964871, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI1790195, Length=477, Percent_Identity=29.1404612159329, Blast_Score=161, Evalue=8e-41,
Organism=Escherichia coli, GI1788710, Length=437, Percent_Identity=26.7734553775744, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI87081983, Length=432, Percent_Identity=26.6203703703704, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1788392, Length=427, Percent_Identity=28.5714285714286, Blast_Score=130, Evalue=3e-31,
Organism=Escherichia coli, GI87082231, Length=217, Percent_Identity=24.8847926267281, Blast_Score=64, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6324954, Length=454, Percent_Identity=23.1277533039648, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6319770, Length=394, Percent_Identity=24.6192893401015, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6325455, Length=366, Percent_Identity=25.4098360655738, Blast_Score=81, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6323520, Length=266, Percent_Identity=25.5639097744361, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323935, Length=260, Percent_Identity=26.1538461538462, Blast_Score=78, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6322958, Length=370, Percent_Identity=22.4324324324324, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323735, Length=336, Percent_Identity=23.8095238095238, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6321663, Length=149, Percent_Identity=27.5167785234899, Blast_Score=72, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 53067; Mature: 52936

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPEQSSVAHVEHLARDRDGHVVTEEQAWSALWALLIGFFMILVDTSIVTTALPATIRAL
CCCCHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NANLNQGVWITSAYLLTYAVPLLITGRMGDRWGPHRMYIIGMLVFGVSSLACGLSPTIGV
CCCCCCCCHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHH
LIAARAVQGIGAALMTPQSLSMITRLFPPEKRGAAMGLWGATAGVASFVGPVLGGILVDT
HHHHHHHHHCCHHHCCCHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHH
LGWEWIFFVNVPVTVIGLWLAIRNVPALETHGHAFDWCGVVLSGIGMTLLVFGIQEGEQY
CCCCEEEEEECHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
NWGTITGIISVPLLIVLGVVFMVLFVVSQTQWAQSRLHARSTNFEPLVPLRLFTKRNFTL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCHHHHHHCCCCHH
ANIAIFLVGMLVTSYFLPTAIYLQNVRGKSPTVAALLLIPTAVVSGVLSPVVGRVLQHRR
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGRMAAIGVSLYVISSIGWWLFTVPSTGLWVFPLLSVITGVGAALTWSPISLVGTDDLGQ
CCCEEEHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHCCCCEEEECCEEEECCCCCCC
ADAGAGSGVYNATRQVGSALGSAFIAMMMDARIMARHDLGRGLGESMFITITAGTICVIV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHH
CAFFANFQRSRQRNS
HHHHHHHHHHHCCCC
>Mature Secondary Structure 
SPEQSSVAHVEHLARDRDGHVVTEEQAWSALWALLIGFFMILVDTSIVTTALPATIRAL
CCCHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NANLNQGVWITSAYLLTYAVPLLITGRMGDRWGPHRMYIIGMLVFGVSSLACGLSPTIGV
CCCCCCCCHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHH
LIAARAVQGIGAALMTPQSLSMITRLFPPEKRGAAMGLWGATAGVASFVGPVLGGILVDT
HHHHHHHHHCCHHHCCCHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHH
LGWEWIFFVNVPVTVIGLWLAIRNVPALETHGHAFDWCGVVLSGIGMTLLVFGIQEGEQY
CCCCEEEEEECHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
NWGTITGIISVPLLIVLGVVFMVLFVVSQTQWAQSRLHARSTNFEPLVPLRLFTKRNFTL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCHHHHHHCCCCHH
ANIAIFLVGMLVTSYFLPTAIYLQNVRGKSPTVAALLLIPTAVVSGVLSPVVGRVLQHRR
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGRMAAIGVSLYVISSIGWWLFTVPSTGLWVFPLLSVITGVGAALTWSPISLVGTDDLGQ
CCCEEEHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHCCCCEEEECCEEEECCCCCCC
ADAGAGSGVYNATRQVGSALGSAFIAMMMDARIMARHDLGRGLGESMFITITAGTICVIV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHH
CAFFANFQRSRQRNS
HHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]