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The map label for this gene is ntcA [H]

Identifier: 50841715

GI number: 50841715

Start: 276567

End: 277268

Strand: Reverse

Name: ntcA [H]

Synonym: PPA0222

Alternate gene names: 50841715

Gene position: 277268-276567 (Counterclockwise)

Preceding gene: 50841719

Following gene: 50841712

Centisome position: 10.83

GC content: 63.68

Gene sequence:

>702_bases
ATGGCAAATGTGGTGGCAACAGCGGCGACCCAACCGTTCTTCGCAACTCTCGGCGCAGCCTCGGCCGCCCGAATCATGGC
CATGTGTGAGGCGTCAGTCTGCCCGCGAAATACCGTGATCTTCGAGGCCGGAGACTCAGCTGACAACCTGTACCTCGTCG
TCGAAGGAAAGGTGAAGCTCACTCGCTCCACAACTGCCGATGACCTCACTCCCCGCGACTCCCTGTTGTGGCTTATGGGC
CCCAGTGACATGTTTGGGGAGCTGTCGCTGGTTGACGGCGGCACCCGCTCCACGACGGCCACCACTGTGACCCGCTGCAG
CCTGCTCAAGACTCCGGGTGCGCAGATGCGCGAGCTCGTTAAAGGCCGTCACGACGTCGCCCTGGCTATGTTGGGACGCC
TGTCGGAGCGCCTGCGTCGAGCCGACGACAACACCGCCCATTTTGTCTCCGGTGACGTCCCAAGTCGATTGGCGTACACC
CTGCTCGACCTGGCACGACGCTTCGGTACCGCGAACCCGTCAACTGGGCACATCGTGGTGCGTCACGACCTCACCCAGCA
CGAGCTCGCCCAGGCCGTCGGCTCCTCCCGAGAGACCGTCAACAAGGCGCTGACGGATTTCGCCGCTCGGGGATGGATCG
CAGCGCGACCCAAGGTTGTCACCATCATGGAGCCGGAGAAGCTGGCATCGCGGGCCAACTGA

Upstream 100 bases:

>100_bases
GCCCGGTGTCAGCACCGAATGTGACAGCTGCCACAATGTAGGCACACGCCCGGCCACGAATGGTCGAACGATGCACGTGC
TCATGAGGGGAGATGTTCGG

Downstream 100 bases:

>100_bases
GTTCGCGGCACCTCACATCCACAAGTGTGGGGGCCAGGCGTTAACGCCTGGCCCCCACAGTGTTGTTCTGGAACCGGCGC
GATCAGACTTCGACGGTCAG

Product: putative transcriptional regulator

Products: NA

Alternate protein names: DNA-binding protein VF1; Nitrogen-responsive regulatory protein [H]

Number of amino acids: Translated: 233; Mature: 232

Protein sequence:

>233_residues
MANVVATAATQPFFATLGAASAARIMAMCEASVCPRNTVIFEAGDSADNLYLVVEGKVKLTRSTTADDLTPRDSLLWLMG
PSDMFGELSLVDGGTRSTTATTVTRCSLLKTPGAQMRELVKGRHDVALAMLGRLSERLRRADDNTAHFVSGDVPSRLAYT
LLDLARRFGTANPSTGHIVVRHDLTQHELAQAVGSSRETVNKALTDFAARGWIAARPKVVTIMEPEKLASRAN

Sequences:

>Translated_233_residues
MANVVATAATQPFFATLGAASAARIMAMCEASVCPRNTVIFEAGDSADNLYLVVEGKVKLTRSTTADDLTPRDSLLWLMG
PSDMFGELSLVDGGTRSTTATTVTRCSLLKTPGAQMRELVKGRHDVALAMLGRLSERLRRADDNTAHFVSGDVPSRLAYT
LLDLARRFGTANPSTGHIVVRHDLTQHELAQAVGSSRETVNKALTDFAARGWIAARPKVVTIMEPEKLASRAN
>Mature_232_residues
ANVVATAATQPFFATLGAASAARIMAMCEASVCPRNTVIFEAGDSADNLYLVVEGKVKLTRSTTADDLTPRDSLLWLMGP
SDMFGELSLVDGGTRSTTATTVTRCSLLKTPGAQMRELVKGRHDVALAMLGRLSERLRRADDNTAHFVSGDVPSRLAYTL
LDLARRFGTANPSTGHIVVRHDLTQHELAQAVGSSRETVNKALTDFAARGWIAARPKVVTIMEPEKLASRAN

Specific function: Has affinity for the xisA upstream region. Binds to a 66 bp region containing three repeats of the consensus recognition sequence 5'-ACATT-3' [H]

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789756, Length=199, Percent_Identity=25.1256281407035, Blast_Score=79, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR022299
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 25028; Mature: 24897

Theoretical pI: Translated: 8.90; Mature: 8.90

Prosite motif: PS50042 CNMP_BINDING_3 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANVVATAATQPFFATLGAASAARIMAMCEASVCPRNTVIFEAGDSADNLYLVVEGKVKL
CCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCEEEEEECCEEE
TRSTTADDLTPRDSLLWLMGPSDMFGELSLVDGGTRSTTATTVTRCSLLKTPGAQMRELV
EECCCCCCCCCCCCEEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHH
KGRHDVALAMLGRLSERLRRADDNTAHFVSGDVPSRLAYTLLDLARRFGTANPSTGHIVV
CCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCEEEE
RHDLTQHELAQAVGSSRETVNKALTDFAARGWIAARPKVVTIMEPEKLASRAN
EECCCHHHHHHHHCCCHHHHHHHHHHHHHCCEEECCCEEEEEECHHHHHCCCC
>Mature Secondary Structure 
ANVVATAATQPFFATLGAASAARIMAMCEASVCPRNTVIFEAGDSADNLYLVVEGKVKL
CCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCEEEEEECCEEE
TRSTTADDLTPRDSLLWLMGPSDMFGELSLVDGGTRSTTATTVTRCSLLKTPGAQMRELV
EECCCCCCCCCCCCEEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHH
KGRHDVALAMLGRLSERLRRADDNTAHFVSGDVPSRLAYTLLDLARRFGTANPSTGHIVV
CCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCEEEE
RHDLTQHELAQAVGSSRETVNKALTDFAARGWIAARPKVVTIMEPEKLASRAN
EECCCHHHHHHHHCCCHHHHHHHHHHHHHCCEEECCCEEEEEECHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11114927 [H]