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The map label for this gene is ygfK [H]

Identifier: 50841651

GI number: 50841651

Start: 196341

End: 198128

Strand: Reverse

Name: ygfK [H]

Synonym: PPA0163

Alternate gene names: 50841651

Gene position: 198128-196341 (Counterclockwise)

Preceding gene: 50841653

Following gene: 50841650

Centisome position: 7.74

GC content: 63.26

Gene sequence:

>1788_bases
ATGAGCGCCGAGACTATGACGAGTGACACCACCGACCCAAACGGGTACGTCATCAGGGAAAACCAGGACATCACTGCACG
TCCTGACCTAGCCCGCACGACCGGCCGCGTCACTCCCACCCGTACCCGCAGACCCGTATACCTCGACATGCTTCCTCCCT
GTAATGCTGGCTGCCCAGCCGGCGAAAACATTCAGGAGTGGCTGCGCCTCATCAAGGCCCACGAAGAAGAGAAGGCATGG
CGTCAACTGACCGCCGACAACCCCTTCCCTGCCATCCACGGTCGTGTCTGCTACCACCCCTGCGAAGTAGCCTGTAACCG
CGCCGACCTCGATGGTGCCGTGTCCATTCACTCCGTGGAGCGCTACCTCGGTGACCTTGCCATTGAAAAGGGCTGGACGT
TCACCAGGCCCACGGAGCCCTCCGGGCACCGCGTCCTCGTCATTGGCGCTGGCCCGGCCGGCCTGTCTGCCGCCTACCAC
CTCGCAATGCTTGGCCACGACGTCGAGATCCACGACGCGGGCGACAAAGTCGGCGGCATGATGCGCTACGGCATCCCCGA
ATACCGTCTACCGCGCGAAATCCTCGACGCCGAAGTAGCACGTCTCACCGACCTCGGCATCAAGATCGTCCTCAACCATC
CCGTCAACGACCTCATGGAAGAAAAGGCCAAGGGGCGTTTCGACGCCGTCTTCGTGGCCATCGGCGCCCATCTATCCAAA
CGCGTCGACATCCCGACCGTTGATGCCTCCCGAATGGTCGACGCCGTGAGCTTTTTGCACAACGTCGCCGCCGGCGAGAA
GCCACTCATTGGACGCCGCGTAGCTGTTTACGGCGGCGGCAACACCGCCATGGATGCCGCGCGCGTCGCCAAGCGCCTCG
GCGCCGAGGAGTCCATCGTCGTCTACCGACGCACCGCAGAGCAGATGCCCGCCCACGCCGAAGAGCGCGAAGAAGCCGAG
CGCGAGGGCGTCCAGATGAACTGGCTGCGCACCATCACTGACGTCGGCGACGACCTCACCGTCGAGGTCATGGAACTCGA
CGAGGACGGCAAGCCGCACGGCACCGGACGCTACGAGAAACTCGAAGCCGACACCGTCATCCTTGCCGTCGGCCAGGACG
CCGACACCAGCTTCCTACACAAGATGCCCGGTATGAGGTTCCGCAACGACGTCGTCGACGTCAACCCCTCAACCCTCATG
ACCGACGTCCCCGGTATCTTCGCCGGCGGCGACACCGTCCCCTCCGAGCGCACCGTGACGATCGGCGTTGGCCACGGCAA
ACGCGCCGCCCGTCAGATCGACCTGTGGCTGAACCGCGAGTCCGGTTACCCGACCGCCAAGAACACCACGGTGGGCTTCA
ACGACCTCAACCTGTGGTACTTCGGCGATCACCTGCGTCGTCACCAGCCCGAACTCGACCCGACCCAGCGCGTCGGATTC
GACGAGGTCGTCGGTGGACTCACCGACGATCAGGCCCACTTCGAAGCCGGACGATGCCTATCCTGCGGTAACTGCTTCGA
ATGTGACGGCTGCTACGGTTCCTGCCCTGAGGATGCCATTATCAAGCTCGGCAAGGGCCACCGCTACGAATTCAACTACG
ACAAATGCACCGGATGCGCCACCTGCTTCGACCAGTGCCCAGTTCATGCTATTGAGATGATCCCCGAAGGCACGGACCCG
GCCACCGTACAGGGATCCGGCAACCCCACACCCGGCTGGGCGGTGCGTCCTGAACCTAATCCACAACGGTCAGCTTCACT
GAAGTTCACCGTCGACTCCGAGAACTGA

Upstream 100 bases:

>100_bases
GCACGCCCTTTCACATGTGGTCTAACATCGGCTGTAGGTTCGTTTGCGAACCCCACATTCGCAATCTGAAATGCCGCATT
TCCAACAGGAAGAGCCAGGT

Downstream 100 bases:

>100_bases
GAAGGAGGACTGACCATGGCAAATAGCTCCGACTCCCGCGTCCGCACCATTGCCGACGGCAACACCGCCGCCGCCTCGGT
CGCCTATCGTTGCAACGAAC

Product: putative glutamate synthase (NADPH) small subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 595; Mature: 594

Protein sequence:

>595_residues
MSAETMTSDTTDPNGYVIRENQDITARPDLARTTGRVTPTRTRRPVYLDMLPPCNAGCPAGENIQEWLRLIKAHEEEKAW
RQLTADNPFPAIHGRVCYHPCEVACNRADLDGAVSIHSVERYLGDLAIEKGWTFTRPTEPSGHRVLVIGAGPAGLSAAYH
LAMLGHDVEIHDAGDKVGGMMRYGIPEYRLPREILDAEVARLTDLGIKIVLNHPVNDLMEEKAKGRFDAVFVAIGAHLSK
RVDIPTVDASRMVDAVSFLHNVAAGEKPLIGRRVAVYGGGNTAMDAARVAKRLGAEESIVVYRRTAEQMPAHAEEREEAE
REGVQMNWLRTITDVGDDLTVEVMELDEDGKPHGTGRYEKLEADTVILAVGQDADTSFLHKMPGMRFRNDVVDVNPSTLM
TDVPGIFAGGDTVPSERTVTIGVGHGKRAARQIDLWLNRESGYPTAKNTTVGFNDLNLWYFGDHLRRHQPELDPTQRVGF
DEVVGGLTDDQAHFEAGRCLSCGNCFECDGCYGSCPEDAIIKLGKGHRYEFNYDKCTGCATCFDQCPVHAIEMIPEGTDP
ATVQGSGNPTPGWAVRPEPNPQRSASLKFTVDSEN

Sequences:

>Translated_595_residues
MSAETMTSDTTDPNGYVIRENQDITARPDLARTTGRVTPTRTRRPVYLDMLPPCNAGCPAGENIQEWLRLIKAHEEEKAW
RQLTADNPFPAIHGRVCYHPCEVACNRADLDGAVSIHSVERYLGDLAIEKGWTFTRPTEPSGHRVLVIGAGPAGLSAAYH
LAMLGHDVEIHDAGDKVGGMMRYGIPEYRLPREILDAEVARLTDLGIKIVLNHPVNDLMEEKAKGRFDAVFVAIGAHLSK
RVDIPTVDASRMVDAVSFLHNVAAGEKPLIGRRVAVYGGGNTAMDAARVAKRLGAEESIVVYRRTAEQMPAHAEEREEAE
REGVQMNWLRTITDVGDDLTVEVMELDEDGKPHGTGRYEKLEADTVILAVGQDADTSFLHKMPGMRFRNDVVDVNPSTLM
TDVPGIFAGGDTVPSERTVTIGVGHGKRAARQIDLWLNRESGYPTAKNTTVGFNDLNLWYFGDHLRRHQPELDPTQRVGF
DEVVGGLTDDQAHFEAGRCLSCGNCFECDGCYGSCPEDAIIKLGKGHRYEFNYDKCTGCATCFDQCPVHAIEMIPEGTDP
ATVQGSGNPTPGWAVRPEPNPQRSASLKFTVDSEN
>Mature_594_residues
SAETMTSDTTDPNGYVIRENQDITARPDLARTTGRVTPTRTRRPVYLDMLPPCNAGCPAGENIQEWLRLIKAHEEEKAWR
QLTADNPFPAIHGRVCYHPCEVACNRADLDGAVSIHSVERYLGDLAIEKGWTFTRPTEPSGHRVLVIGAGPAGLSAAYHL
AMLGHDVEIHDAGDKVGGMMRYGIPEYRLPREILDAEVARLTDLGIKIVLNHPVNDLMEEKAKGRFDAVFVAIGAHLSKR
VDIPTVDASRMVDAVSFLHNVAAGEKPLIGRRVAVYGGGNTAMDAARVAKRLGAEESIVVYRRTAEQMPAHAEEREEAER
EGVQMNWLRTITDVGDDLTVEVMELDEDGKPHGTGRYEKLEADTVILAVGQDADTSFLHKMPGMRFRNDVVDVNPSTLMT
DVPGIFAGGDTVPSERTVTIGVGHGKRAARQIDLWLNRESGYPTAKNTTVGFNDLNLWYFGDHLRRHQPELDPTQRVGFD
EVVGGLTDDQAHFEAGRCLSCGNCFECDGCYGSCPEDAIIKLGKGHRYEFNYDKCTGCATCFDQCPVHAIEMIPEGTDPA
TVQGSGNPTPGWAVRPEPNPQRSASLKFTVDSEN

Specific function: Unknown

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=433, Percent_Identity=27.0207852193995, Blast_Score=132, Evalue=1e-30,
Organism=Escherichia coli, GI1789243, Length=522, Percent_Identity=32.3754789272031, Blast_Score=192, Evalue=5e-50,
Organism=Escherichia coli, GI1788468, Length=390, Percent_Identity=31.025641025641, Blast_Score=175, Evalue=6e-45,
Organism=Escherichia coli, GI87082180, Length=439, Percent_Identity=31.6628701594533, Blast_Score=175, Evalue=6e-45,
Organism=Escherichia coli, GI1788811, Length=438, Percent_Identity=31.9634703196347, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI1789606, Length=421, Percent_Identity=31.8289786223278, Blast_Score=172, Evalue=8e-44,
Organism=Caenorhabditis elegans, GI17570289, Length=442, Percent_Identity=26.2443438914027, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI71984108, Length=432, Percent_Identity=28.2407407407407, Blast_Score=121, Evalue=8e-28,
Organism=Saccharomyces cerevisiae, GI6320030, Length=439, Percent_Identity=26.4236902050114, Blast_Score=126, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24665547, Length=439, Percent_Identity=29.1571753986333, Blast_Score=155, Evalue=9e-38,
Organism=Drosophila melanogaster, GI24665543, Length=439, Percent_Identity=29.1571753986333, Blast_Score=155, Evalue=9e-38,
Organism=Drosophila melanogaster, GI28574881, Length=439, Percent_Identity=29.1571753986333, Blast_Score=154, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24665539, Length=439, Percent_Identity=29.1571753986333, Blast_Score=154, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24640763, Length=419, Percent_Identity=27.6849642004773, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI18858217, Length=419, Percent_Identity=27.6849642004773, Blast_Score=120, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR013785
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR017701 [H]

Pfam domain/function: PF07992 Pyr_redox_2 [H]

EC number: NA

Molecular weight: Translated: 65300; Mature: 65169

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAETMTSDTTDPNGYVIRENQDITARPDLARTTGRVTPTRTRRPVYLDMLPPCNAGCPA
CCCCCCCCCCCCCCCEEEECCCCCCCCCCHHHHCCCCCCCCCCCCEEEEECCCCCCCCCC
GENIQEWLRLIKAHEEEKAWRQLTADNPFPAIHGRVCYHPCEVACNRADLDGAVSIHSVE
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECHHHHHCCCCCCCCCEEHHHHH
RYLGDLAIEKGWTFTRPTEPSGHRVLVIGAGPAGLSAAYHLAMLGHDVEIHDAGDKVGGM
HHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEECCCCHHCCH
MRYGIPEYRLPREILDAEVARLTDLGIKIVLNHPVNDLMEEKAKGRFDAVFVAIGAHLSK
HHCCCCCCCCCHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHCCCCEEEEEEEHHHHHC
RVDIPTVDASRMVDAVSFLHNVAAGEKPLIGRRVAVYGGGNTAMDAARVAKRLGAEESIV
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCEEE
VYRRTAEQMPAHAEEREEAEREGVQMNWLRTITDVGDDLTVEVMELDEDGKPHGTGRYEK
EEECCHHHCCCCHHHHHHHHHCCCEEHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCEE
LEADTVILAVGQDADTSFLHKMPGMRFRNDVVDVNPSTLMTDVPGIFAGGDTVPSERTVT
ECCCEEEEEECCCCCHHHHHHCCCCCCCCCEEECCCCHHHCCCCCEEECCCCCCCCCEEE
IGVGHGKRAARQIDLWLNRESGYPTAKNTTVGFNDLNLWYFGDHLRRHQPELDPTQRVGF
EECCCCCHHHEEEEEEEECCCCCCCCCCCEECCCCCEEEEECCHHHHCCCCCCHHHHCCH
DEVVGGLTDDQAHFEAGRCLSCGNCFECDGCYGSCPEDAIIKLGKGHRYEFNYDKCTGCA
HHHHCCCCCCHHHHCCCCEECCCCEEECCCCCCCCCHHHHHCCCCCCEEEECCHHHHHHH
TCFDQCPVHAIEMIPEGTDPATVQGSGNPTPGWAVRPEPNPQRSASLKFTVDSEN
HHHHHCCCHHHHHCCCCCCCCEEECCCCCCCCCEECCCCCCCCCCEEEEEECCCC
>Mature Secondary Structure 
SAETMTSDTTDPNGYVIRENQDITARPDLARTTGRVTPTRTRRPVYLDMLPPCNAGCPA
CCCCCCCCCCCCCCEEEECCCCCCCCCCHHHHCCCCCCCCCCCCEEEEECCCCCCCCCC
GENIQEWLRLIKAHEEEKAWRQLTADNPFPAIHGRVCYHPCEVACNRADLDGAVSIHSVE
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECHHHHHCCCCCCCCCEEHHHHH
RYLGDLAIEKGWTFTRPTEPSGHRVLVIGAGPAGLSAAYHLAMLGHDVEIHDAGDKVGGM
HHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEECCCCHHCCH
MRYGIPEYRLPREILDAEVARLTDLGIKIVLNHPVNDLMEEKAKGRFDAVFVAIGAHLSK
HHCCCCCCCCCHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHCCCCEEEEEEEHHHHHC
RVDIPTVDASRMVDAVSFLHNVAAGEKPLIGRRVAVYGGGNTAMDAARVAKRLGAEESIV
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCEEE
VYRRTAEQMPAHAEEREEAEREGVQMNWLRTITDVGDDLTVEVMELDEDGKPHGTGRYEK
EEECCHHHCCCCHHHHHHHHHCCCEEHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCEE
LEADTVILAVGQDADTSFLHKMPGMRFRNDVVDVNPSTLMTDVPGIFAGGDTVPSERTVT
ECCCEEEEEECCCCCHHHHHHCCCCCCCCCEEECCCCHHHCCCCCEEECCCCCCCCCEEE
IGVGHGKRAARQIDLWLNRESGYPTAKNTTVGFNDLNLWYFGDHLRRHQPELDPTQRVGF
EECCCCCHHHEEEEEEEECCCCCCCCCCCEECCCCCEEEEECCHHHHCCCCCCHHHHCCH
DEVVGGLTDDQAHFEAGRCLSCGNCFECDGCYGSCPEDAIIKLGKGHRYEFNYDKCTGCA
HHHHCCCCCCHHHHCCCCEECCCCEEECCCCCCCCCHHHHHCCCCCCEEEECCHHHHHHH
TCFDQCPVHAIEMIPEGTDPATVQGSGNPTPGWAVRPEPNPQRSASLKFTVDSEN
HHHHHCCCHHHHHCCCCCCCCEEECCCCCCCCCEECCCCCCCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Iron-Sulfur [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]