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The map label for this gene is wcaL [H]

Identifier: 50841619

GI number: 50841619

Start: 156026

End: 157288

Strand: Reverse

Name: wcaL [H]

Synonym: PPA0130

Alternate gene names: 50841619

Gene position: 157288-156026 (Counterclockwise)

Preceding gene: 50841622

Following gene: 50841613

Centisome position: 6.14

GC content: 61.44

Gene sequence:

>1263_bases
ATGGCACATCGAGAGTCGACGTTGATCCTCCTGACGAACACCTACCCGCTGTCACGCGGGGAGGAGTTCCTTGAGAACGA
GATCGACCATGTGGGGCGCGCCTTCGATCAGGTGGTGATCATCCCAATCCAGGCTGGTCCCGCCGATGTGCAGACACGAG
CCATCCCGAGCAACGCTCAGGTCATGCACATTGCCCGGTCTTCACCCCGGGGCCTCGATCGCGCCCTCGCCGCCGTGAAA
GGGCTAGTTCGGCTGCCACCCAGCGGAGTGGACTGGGCAGCGACACGACGACAGCCCCGCCTACTCGTCTCTGACGCTCA
CTTCGAGGCTTGCGCCCAAACAGCCCTCGACAGCATCCTTGCCAAACTCCCGAACCTCAGGCTGACCTCGGAATCCCATG
TCACAATCTATTCCTTCTGGCTCCATATCACAGCCCGCACCGCAACCCTGCTAGCTGAGCAGTTGCGCGCCCAAGGAGTC
ACGGTGGATCGCGTAATCTCCCGTGCCCATCGCTACGACTTGTACCCCAGCGTTGCCCCTCGTCACCACATCCCAGAACG
TCGCCTGCTATTGCAGTCTCTTGACGGGATCTGCCCGGTCTCCGAGCAGGGGACTCGGGAACTGCAAACAACGTGGCCCC
AGTACGCCGGGAAGATACACACTCGTTACCTGGGCACTTCCGACCCCGGACCGACGGCCCACTGCCGACGTGACCCATTC
ACCATTGTCAGCTGTGCCCATCTTGTCCCAGTCAAGAGAATGATCCGGATGCCCGCGATCCTGGCCAGGGTGCGAGCTAG
CGGCATCGACGCACGCTGGACTCATCTGGGTGACGGCCCACAGATGGACACTCTGCGCGAGGAGGTCACCGTTCACCGCG
TCACGGATGCTGTCACCCTGCTCGGTCACGTCGACAACACCCAGGTCATGGCGACCCAGCGCGATCTCAAACCGTCAGTA
TTCGTCAACCTCTCCTCCTCGGAAGGACTTCCTGTATCAATGATGGAGGTTGCCTCCCTCGGTATCCCCATTATCGCGAC
TGGCGTCGGCGGAGTAGGAGAAATCGTCTCGTCTGACAACGGGCATCTATTGCCTGCCGAGTTCACCGACGCCCAGGCAT
CTGACGCGTTAGTGCAGCTGGCACGTCTGTCTGAGGACGAGTACCAGCAGGTGTGTCAGGCCTCCCGCCAGGTGTGGGAA
GAAAAGTTCCGCGCCTCTGTCGTCTACCCCGAATTCTGTCGCGAGGTGCTGGGCGGACGCTGA

Upstream 100 bases:

>100_bases
GCACGGGCTCCCTCCACCAACCGTATTGCCACACGTAGTCGACCAGGCGACCACCCCACGCCCCGCGAGGATGACGACCC
TGGCACGTTAGGATCACCAC

Downstream 100 bases:

>100_bases
TCAGTCCACGCCACGCTCGACAGGGTGACCACCCACCACCACATACTCACGCCGAAGTTTTTGACCAAGTAAGTATCAAC
GAGGAAAGCTGGCAACGTGG

Product: putative glycosyl transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 420; Mature: 419

Protein sequence:

>420_residues
MAHRESTLILLTNTYPLSRGEEFLENEIDHVGRAFDQVVIIPIQAGPADVQTRAIPSNAQVMHIARSSPRGLDRALAAVK
GLVRLPPSGVDWAATRRQPRLLVSDAHFEACAQTALDSILAKLPNLRLTSESHVTIYSFWLHITARTATLLAEQLRAQGV
TVDRVISRAHRYDLYPSVAPRHHIPERRLLLQSLDGICPVSEQGTRELQTTWPQYAGKIHTRYLGTSDPGPTAHCRRDPF
TIVSCAHLVPVKRMIRMPAILARVRASGIDARWTHLGDGPQMDTLREEVTVHRVTDAVTLLGHVDNTQVMATQRDLKPSV
FVNLSSSEGLPVSMMEVASLGIPIIATGVGGVGEIVSSDNGHLLPAEFTDAQASDALVQLARLSEDEYQQVCQASRQVWE
EKFRASVVYPEFCREVLGGR

Sequences:

>Translated_420_residues
MAHRESTLILLTNTYPLSRGEEFLENEIDHVGRAFDQVVIIPIQAGPADVQTRAIPSNAQVMHIARSSPRGLDRALAAVK
GLVRLPPSGVDWAATRRQPRLLVSDAHFEACAQTALDSILAKLPNLRLTSESHVTIYSFWLHITARTATLLAEQLRAQGV
TVDRVISRAHRYDLYPSVAPRHHIPERRLLLQSLDGICPVSEQGTRELQTTWPQYAGKIHTRYLGTSDPGPTAHCRRDPF
TIVSCAHLVPVKRMIRMPAILARVRASGIDARWTHLGDGPQMDTLREEVTVHRVTDAVTLLGHVDNTQVMATQRDLKPSV
FVNLSSSEGLPVSMMEVASLGIPIIATGVGGVGEIVSSDNGHLLPAEFTDAQASDALVQLARLSEDEYQQVCQASRQVWE
EKFRASVVYPEFCREVLGGR
>Mature_419_residues
AHRESTLILLTNTYPLSRGEEFLENEIDHVGRAFDQVVIIPIQAGPADVQTRAIPSNAQVMHIARSSPRGLDRALAAVKG
LVRLPPSGVDWAATRRQPRLLVSDAHFEACAQTALDSILAKLPNLRLTSESHVTIYSFWLHITARTATLLAEQLRAQGVT
VDRVISRAHRYDLYPSVAPRHHIPERRLLLQSLDGICPVSEQGTRELQTTWPQYAGKIHTRYLGTSDPGPTAHCRRDPFT
IVSCAHLVPVKRMIRMPAILARVRASGIDARWTHLGDGPQMDTLREEVTVHRVTDAVTLLGHVDNTQVMATQRDLKPSVF
VNLSSSEGLPVSMMEVASLGIPIIATGVGGVGEIVSSDNGHLLPAEFTDAQASDALVQLARLSEDEYQQVCQASRQVWEE
KFRASVVYPEFCREVLGGR

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG0438

COG function: function code M; Glycosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1788357, Length=275, Percent_Identity=26.5454545454545, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296 [H]

Pfam domain/function: PF00534 Glycos_transf_1 [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 46316; Mature: 46184

Theoretical pI: Translated: 7.26; Mature: 7.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHRESTLILLTNTYPLSRGEEFLENEIDHVGRAFDQVVIIPIQAGPADVQTRAIPSNAQ
CCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCCEEEECCCCCE
VMHIARSSPRGLDRALAAVKGLVRLPPSGVDWAATRRQPRLLVSDAHFEACAQTALDSIL
EEEEECCCCCHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCEEEECHHHHHHHHHHHHHHH
AKLPNLRLTSESHVTIYSFWLHITARTATLLAEQLRAQGVTVDRVISRAHRYDLYPSVAP
HHCCCCEECCCCCEEEEEEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCC
RHHIPERRLLLQSLDGICPVSEQGTRELQTTWPQYAGKIHTRYLGTSDPGPTAHCRRDPF
CCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCC
TIVSCAHLVPVKRMIRMPAILARVRASGIDARWTHLGDGPQMDTLREEVTVHRVTDAVTL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHH
LGHVDNTQVMATQRDLKPSVFVNLSSSEGLPVSMMEVASLGIPIIATGVGGVGEIVSSDN
HHCCCCCEEEEECCCCCCEEEEEECCCCCCCHHHHHHHHCCCCEEEECCCCHHHHHCCCC
GHLLPAEFTDAQASDALVQLARLSEDEYQQVCQASRQVWEEKFRASVVYPEFCREVLGGR
CCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCC
>Mature Secondary Structure 
AHRESTLILLTNTYPLSRGEEFLENEIDHVGRAFDQVVIIPIQAGPADVQTRAIPSNAQ
CCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCCEEEECCCCCE
VMHIARSSPRGLDRALAAVKGLVRLPPSGVDWAATRRQPRLLVSDAHFEACAQTALDSIL
EEEEECCCCCHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCEEEECHHHHHHHHHHHHHHH
AKLPNLRLTSESHVTIYSFWLHITARTATLLAEQLRAQGVTVDRVISRAHRYDLYPSVAP
HHCCCCEECCCCCEEEEEEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCC
RHHIPERRLLLQSLDGICPVSEQGTRELQTTWPQYAGKIHTRYLGTSDPGPTAHCRRDPF
CCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCC
TIVSCAHLVPVKRMIRMPAILARVRASGIDARWTHLGDGPQMDTLREEVTVHRVTDAVTL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHH
LGHVDNTQVMATQRDLKPSVFVNLSSSEGLPVSMMEVASLGIPIIATGVGGVGEIVSSDN
HHCCCCCEEEEECCCCCCEEEEEECCCCCCCHHHHHHHHCCCCEEEECCCCHHHHHCCCC
GHLLPAEFTDAQASDALVQLARLSEDEYQQVCQASRQVWEEKFRASVVYPEFCREVLGGR
CCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]