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The map label for this gene is rnjA [H]

Identifier: 50365387

GI number: 50365387

Start: 662347

End: 664113

Strand: Direct

Name: rnjA [H]

Synonym: Mfl570

Alternate gene names: 50365387

Gene position: 662347-664113 (Clockwise)

Preceding gene: 50365386

Following gene: 50365391

Centisome position: 83.5

GC content: 29.26

Gene sequence:

>1767_bases
ATGGAAAAGGAAAAAATAACTTCTACTAATCAAATTATTGAAAAAGAAGATTTTGAACCTTTTGTTTTTAAACAAAAAGA
AGTTCAAAGAAAATATATTAAAACTGAATCACCAACTAAAGTTTTTGCTTTAGGTGGACTTGAAGAAATTGGTAAAAATA
CTTACTGTATAGAACATGAAAACGAAATTATTATGATTGATGCTGGAGTTAAGTTCCCAGATGATTCTATGTTAGGTATT
AATGCAGTTATCCCTGATTATTCATATTTAAAAGAAAATGAATCAAAAATAAAAGCGCTTTTCATAACTCATGGACATGA
AGATCACATTGGTGGAATTCACTATTTAGTTAAACAAGTTAATATACCTGTTATTTATGCACCTGAATTAGCTGCTGCTT
TAATTAGAGATAGATTGAAAGAACATAAATTAACTGACAAAACAATTGTTAAAGAATACGTTGCTGATGATATATGAGCA
ACTAAAAATTTACGTGTAAGTTATGCAGCTTTAAACCACTCTATTCCTGATGCTTTTGGTATTTTAGTAGAAACACCAAA
TGGAAATATTTTTTCAACAGGGGATTATAAATTTGACTGATCACCTTTAGGACACTTTGCTGAATTAAGTAAATTAGCCA
GCATGGGTGATAAAGGAATTGAACTTTTAATGTCTGATTCAACAAATGCTGAAGTTGAAGGTTATACACCTGGTGAAAAA
GGAATTATTGAAAATATCGATAAATTGTTCTTAAAAGCAAAAGGTAGAATTTTTATTACAACTTTTGCTTCTAACGTTCA
CCGTATTCAACAAATTGTTGAGTTAGCTAACAAATATGATAAAAAAGTTGTTATCCTTGGAAGATCAATTGAAAGAATTA
TTAAAATAATCCGTCAAATGGGTCACTTAAAAATTAATGATAAAATGTTTATTAAAGCAAATGACATTGATAAACATCCA
GCAAATAAAATAATGATTATTTCTACAGGTAGCCAAGGTGAACCAATGGCAGCTCTTTCAAGAATTGCTACAAATAGACA
CCAATCTATTTCTATTATTCCTGGTGATACAGTTATTTTCTCTTCATCACCAATACCTGGAAATAGAGCTGATGTTGAAA
TCTTAATTAATAGACTAACTAGAATTGGTGCTAATGTTATTGAATCTTCACCAAGCAACAGAATTCATACTTCAGGTCAC
GCTAGTCAAGAAGAGCAAAAATTATTATTTACATTATTAAGACCAAATTACTTTATGCCAATGCATGGTGAGTTCAGAAT
GTTAAAAAAACATATTGAAACAGCTGAATCTGTTAACCTACAAAAAGGGCATGGATTTGTTATGGCAAATGGTGATCAAT
TAGAATTATTACAAGGTAATGCTCAAATTGGTAAACGTGTTGATGCTGATGCTATTTATGTAGATGGAAAAGATATGACA
GCTCATGCTTCAAACATAATTAGAGAACGTGACATATTAAGTAAAGACGGTTTAATTTCAGTTGTTGTTTCAATTGATTC
TCAAACTAATAAATTATTATGTGCGCCCAGAATTATTTCACGTGGAAGTTTTTATGTAAAAGATTCAGGTAATGTAATTA
GTGAAGCAATTACAATTGTTACAGAAGCTATAAATGAAGTGCTAAATTCTAATAAACCTACATTTGGTGCAATAAAAAAA
GCGATTAAGCAATCACTTTCTCCATATATATTTAAAACTAAACGTAGAAATCCATTAATCATTCCAGTTATATTAAACAA
AAAATAA

Upstream 100 bases:

>100_bases
AAGTCATTATATTTTAATTAATAATCATAAAAATGAAAATTTAATAGTTAAAACATAAGTAATATTTATGTATGTTTATT
TTATATAAAATGGAGGAAAA

Downstream 100 bases:

>100_bases
GAGTTTTTAAACTCTTATTTTTTTAATAATTAATGTTTGTGATTTTTAACTTTTTAATATTTTTTGAATATTTAATAGAA
TTCTTTAAGTCTTTTCGTGT

Product: putative metallo-beta lactamase hydrolase

Products: NA

Alternate protein names: RNase J1 [H]

Number of amino acids: Translated: 588; Mature: 588

Protein sequence:

>588_residues
MEKEKITSTNQIIEKEDFEPFVFKQKEVQRKYIKTESPTKVFALGGLEEIGKNTYCIEHENEIIMIDAGVKFPDDSMLGI
NAVIPDYSYLKENESKIKALFITHGHEDHIGGIHYLVKQVNIPVIYAPELAAALIRDRLKEHKLTDKTIVKEYVADDIWA
TKNLRVSYAALNHSIPDAFGILVETPNGNIFSTGDYKFDWSPLGHFAELSKLASMGDKGIELLMSDSTNAEVEGYTPGEK
GIIENIDKLFLKAKGRIFITTFASNVHRIQQIVELANKYDKKVVILGRSIERIIKIIRQMGHLKINDKMFIKANDIDKHP
ANKIMIISTGSQGEPMAALSRIATNRHQSISIIPGDTVIFSSSPIPGNRADVEILINRLTRIGANVIESSPSNRIHTSGH
ASQEEQKLLFTLLRPNYFMPMHGEFRMLKKHIETAESVNLQKGHGFVMANGDQLELLQGNAQIGKRVDADAIYVDGKDMT
AHASNIIRERDILSKDGLISVVVSIDSQTNKLLCAPRIISRGSFYVKDSGNVISEAITIVTEAINEVLNSNKPTFGAIKK
AIKQSLSPYIFKTKRRNPLIIPVILNKK

Sequences:

>Translated_588_residues
MEKEKITSTNQIIEKEDFEPFVFKQKEVQRKYIKTESPTKVFALGGLEEIGKNTYCIEHENEIIMIDAGVKFPDDSMLGI
NAVIPDYSYLKENESKIKALFITHGHEDHIGGIHYLVKQVNIPVIYAPELAAALIRDRLKEHKLTDKTIVKEYVADDI*A
TKNLRVSYAALNHSIPDAFGILVETPNGNIFSTGDYKFD*SPLGHFAELSKLASMGDKGIELLMSDSTNAEVEGYTPGEK
GIIENIDKLFLKAKGRIFITTFASNVHRIQQIVELANKYDKKVVILGRSIERIIKIIRQMGHLKINDKMFIKANDIDKHP
ANKIMIISTGSQGEPMAALSRIATNRHQSISIIPGDTVIFSSSPIPGNRADVEILINRLTRIGANVIESSPSNRIHTSGH
ASQEEQKLLFTLLRPNYFMPMHGEFRMLKKHIETAESVNLQKGHGFVMANGDQLELLQGNAQIGKRVDADAIYVDGKDMT
AHASNIIRERDILSKDGLISVVVSIDSQTNKLLCAPRIISRGSFYVKDSGNVISEAITIVTEAINEVLNSNKPTFGAIKK
AIKQSLSPYIFKTKRRNPLIIPVILNKK
>Mature_588_residues
MEKEKITSTNQIIEKEDFEPFVFKQKEVQRKYIKTESPTKVFALGGLEEIGKNTYCIEHENEIIMIDAGVKFPDDSMLGI
NAVIPDYSYLKENESKIKALFITHGHEDHIGGIHYLVKQVNIPVIYAPELAAALIRDRLKEHKLTDKTIVKEYVADDI*A
TKNLRVSYAALNHSIPDAFGILVETPNGNIFSTGDYKFD*SPLGHFAELSKLASMGDKGIELLMSDSTNAEVEGYTPGEK
GIIENIDKLFLKAKGRIFITTFASNVHRIQQIVELANKYDKKVVILGRSIERIIKIIRQMGHLKINDKMFIKANDIDKHP
ANKIMIISTGSQGEPMAALSRIATNRHQSISIIPGDTVIFSSSPIPGNRADVEILINRLTRIGANVIESSPSNRIHTSGH
ASQEEQKLLFTLLRPNYFMPMHGEFRMLKKHIETAESVNLQKGHGFVMANGDQLELLQGNAQIGKRVDADAIYVDGKDMT
AHASNIIRERDILSKDGLISVVVSIDSQTNKLLCAPRIISRGSFYVKDSGNVISEAITIVTEAINEVLNSNKPTFGAIKK
AIKQSLSPYIFKTKRRNPLIIPVILNKK

Specific function: Cleaves the 5'-leader sequence of certain mRNAs. May play a role in maturation and stability of specific mRNAs. Is also required for the maturation of the 16S rRNA. Acts preferentially on 16S rRNA precursors after association of the 30S and 50S ribosomal

COG id: COG0595

COG function: function code R; Predicted hydrolase of the metallo-beta-lactamase superfamily

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. RNase J subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR011108
- InterPro:   IPR004613
- InterPro:   IPR001587 [H]

Pfam domain/function: PF00753 Lactamase_B; PF07521 RMMBL [H]

EC number: NA

Molecular weight: Translated: 65313; Mature: 65313

Theoretical pI: Translated: 9.14; Mature: 9.14

Prosite motif: PS00136 SUBTILASE_ASP ; PS01292 UPF0036

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKEKITSTNQIIEKEDFEPFVFKQKEVQRKYIKTESPTKVFALGGLEEIGKNTYCIEHE
CCCCCCCCHHHHHHHCCCCCCEECHHHHHHHHHCCCCCCEEEEECCHHHHCCCEEEEECC
NEIIMIDAGVKFPDDSMLGINAVIPDYSYLKENESKIKALFITHGHEDHIGGIHYLVKQV
CCEEEEECCCCCCCCCCEEEEEECCCHHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHHC
NIPVIYAPELAAALIRDRLKEHKLTDKTIVKEYVADDIATKNLRVSYAALNHSIPDAFGI
CCCEEECHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEE
LVETPNGNIFSTGDYKFDSPLGHFAELSKLASMGDKGIELLMSDSTNAEVEGYTPGEKGI
EEECCCCCEEECCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCEEECCCCCCCCH
IENIDKLFLKAKGRIFITTFASNVHRIQQIVELANKYDKKVVILGRSIERIIKIIRQMGH
HHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHCC
LKINDKMFIKANDIDKHPANKIMIISTGSQGEPMAALSRIATNRHQSISIIPGDTVIFSS
EEECCEEEEEECCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCEEEEECCCEEEEEC
SPIPGNRADVEILINRLTRIGANVIESSPSNRIHTSGHASQEEQKLLFTLLRPNYFMPMH
CCCCCCCCHHHHHHHHHHHHCHHHHHCCCCCCEECCCCCCHHHHHHHHHHHCCCEECCCC
GEFRMLKKHIETAESVNLQKGHGFVMANGDQLELLQGNAQIGKRVDADAIYVDGKDMTAH
CHHHHHHHHHHHHHHCCEECCCEEEEECCCEEEEEECCHHHCCCCCCCEEEECCCCCHHH
ASNIIRERDILSKDGLISVVVSIDSQTNKLLCAPRIISRGSFYVKDSGNVISEAITIVTE
HHHHHHHHHHCCCCCEEEEEEEECCCCCCEEECCHHHCCCCEEEECCCCHHHHHHHHHHH
AINEVLNSNKPTFGAIKKAIKQSLSPYIFKTKRRNPLIIPVILNKK
HHHHHHHCCCCCHHHHHHHHHHCCCCEEEEECCCCCEEEEEEECCC
>Mature Secondary Structure
MEKEKITSTNQIIEKEDFEPFVFKQKEVQRKYIKTESPTKVFALGGLEEIGKNTYCIEHE
CCCCCCCCHHHHHHHCCCCCCEECHHHHHHHHHCCCCCCEEEEECCHHHHCCCEEEEECC
NEIIMIDAGVKFPDDSMLGINAVIPDYSYLKENESKIKALFITHGHEDHIGGIHYLVKQV
CCEEEEECCCCCCCCCCEEEEEECCCHHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHHC
NIPVIYAPELAAALIRDRLKEHKLTDKTIVKEYVADDIATKNLRVSYAALNHSIPDAFGI
CCCEEECHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEE
LVETPNGNIFSTGDYKFDSPLGHFAELSKLASMGDKGIELLMSDSTNAEVEGYTPGEKGI
EEECCCCCEEECCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCEEECCCCCCCCH
IENIDKLFLKAKGRIFITTFASNVHRIQQIVELANKYDKKVVILGRSIERIIKIIRQMGH
HHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHCC
LKINDKMFIKANDIDKHPANKIMIISTGSQGEPMAALSRIATNRHQSISIIPGDTVIFSS
EEECCEEEEEECCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCEEEEECCCEEEEEC
SPIPGNRADVEILINRLTRIGANVIESSPSNRIHTSGHASQEEQKLLFTLLRPNYFMPMH
CCCCCCCCHHHHHHHHHHHHCHHHHHCCCCCCEECCCCCCHHHHHHHHHHHCCCEECCCC
GEFRMLKKHIETAESVNLQKGHGFVMANGDQLELLQGNAQIGKRVDADAIYVDGKDMTAH
CHHHHHHHHHHHHHHCCEECCCEEEEECCCEEEEEECCHHHCCCCCCCEEEECCCCCHHH
ASNIIRERDILSKDGLISVVVSIDSQTNKLLCAPRIISRGSFYVKDSGNVISEAITIVTE
HHHHHHHHHHCCCCCEEEEEEEECCCCCCEEECCHHHCCCCEEEECCCCHHHHHHHHHHH
AINEVLNSNKPTFGAIKKAIKQSLSPYIFKTKRRNPLIIPVILNKK
HHHHHHHCCCCCHHHHHHHHHHCCCCEEEEECCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969500; 9384377 [H]