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The map label for this gene is def2 [H]

Identifier: 50365386

GI number: 50365386

Start: 661596

End: 662198

Strand: Direct

Name: def2 [H]

Synonym: Mfl569

Alternate gene names: 50365386

Gene position: 661596-662198 (Clockwise)

Preceding gene: 50365361

Following gene: 50365387

Centisome position: 83.41

GC content: 29.85

Gene sequence:

>603_bases
ATGAGTATAAAAAATTATTTATTACAAAATGAAATACCAACTAACGAATGATTAACAAAAGATGATAACAAAGAAGTAAT
TAGATCTGTTTCTACAAAAGTAGCAGATGTTAATAACTTATCAAAAGATGAAGAACTAACAATGAAAAAATTAATTGATT
TTGTTGTAGCTAGTCAAGACGAAGAATTTAATTATGAAGGTAAAGAAGACTATTTACGTCCCGCTGTAGGGCTTGCCGCA
CCTCAAATTGGTGTAAATAAAGATATGTTTTATATTAGATTTAATTTACCAAACAACCAAATAGAAGAGTATGCAATGAT
TAACACAGAATACCTGGCAAAATCATCAAGAATGGCAGCATTAGAAGAAGGCGAAGGATGTTTAAGTGTTGATGAAGACA
AACATGGAATTGTCCCAAGATCTTGAATAATTTCTGTTAAGGGGTTTGACTGATTAAAAAAAGAATGAGTCGAATTAAAA
CTTAAAGACTATCGCTCTATTGTTTTTCAACATGAAATGGACCACAATATTGGTAACTTATACTATGACCATATTAATGT
TAATGAACCTGAATTTATTGGCGAAGATTGAGTTGTTCTTTAA

Upstream 100 bases:

>100_bases
AGTAGTTATCGATGAACCTAATTATTTTTTAAAATGATTTTAAGATATAATAATAAATAACGAGAATACAATAAGTTTTT
TAACATGAAAGGATGATATT

Downstream 100 bases:

>100_bases
TTTATGATATTATCAATTTAACAAAAAACAAATTACATATTAAATGTTAAGTCATTATATTTTAATTAATAATCATAAAA
ATGAAAATTTAATAGTTAAA

Product: polypeptide deformylase

Products: NA

Alternate protein names: PDF 2; Polypeptide deformylase 2 [H]

Number of amino acids: Translated: 200; Mature: 199

Protein sequence:

>200_residues
MSIKNYLLQNEIPTNEWLTKDDNKEVIRSVSTKVADVNNLSKDEELTMKKLIDFVVASQDEEFNYEGKEDYLRPAVGLAA
PQIGVNKDMFYIRFNLPNNQIEEYAMINTEYLAKSSRMAALEEGEGCLSVDEDKHGIVPRSWIISVKGFDWLKKEWVELK
LKDYRSIVFQHEMDHNIGNLYYDHINVNEPEFIGEDWVVL

Sequences:

>Translated_200_residues
MSIKNYLLQNEIPTNE*LTKDDNKEVIRSVSTKVADVNNLSKDEELTMKKLIDFVVASQDEEFNYEGKEDYLRPAVGLAA
PQIGVNKDMFYIRFNLPNNQIEEYAMINTEYLAKSSRMAALEEGEGCLSVDEDKHGIVPRS*IISVKGFD*LKKE*VELK
LKDYRSIVFQHEMDHNIGNLYYDHINVNEPEFIGED*VVL
>Mature_199_residues
SIKNYLLQNEIPTNE*LTKDDNKEVIRSVSTKVADVNNLSKDEELTMKKLIDFVVASQDEEFNYEGKEDYLRPAVGLAAP
QIGVNKDMFYIRFNLPNNQIEEYAMINTEYLAKSSRMAALEEGEGCLSVDEDKHGIVPRS*IISVKGFD*LKKE*VELKL
KDYRSIVFQHEMDHNIGNLYYDHINVNEPEFIGED*VVL

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Escherichia coli, GI1789682, Length=117, Percent_Identity=34.1880341880342, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 22293; Mature: 22162

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIKNYLLQNEIPTNELTKDDNKEVIRSVSTKVADVNNLSKDEELTMKKLIDFVVASQDE
CCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCC
EFNYEGKEDYLRPAVGLAAPQIGVNKDMFYIRFNLPNNQIEEYAMINTEYLAKSSRMAAL
CCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
EEGEGCLSVDEDKHGIVPRSIISVKGFDLKKEVELKLKDYRSIVFQHEMDHNIGNLYYDH
HCCCCCEECCCCCCCCCCHHEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEE
INVNEPEFIGEDVVL
CCCCCCCCCCCCCCC
>Mature Secondary Structure 
SIKNYLLQNEIPTNELTKDDNKEVIRSVSTKVADVNNLSKDEELTMKKLIDFVVASQDE
CHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCC
EFNYEGKEDYLRPAVGLAAPQIGVNKDMFYIRFNLPNNQIEEYAMINTEYLAKSSRMAAL
CCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
EEGEGCLSVDEDKHGIVPRSIISVKGFDLKKEVELKLKDYRSIVFQHEMDHNIGNLYYDH
HCCCCCEECCCCCCCCCCHHEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEE
INVNEPEFIGEDVVL
CCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]