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The map label for this gene is obgE [H]

Identifier: 50365339

GI number: 50365339

Start: 613864

End: 615162

Strand: Reverse

Name: obgE [H]

Synonym: Mfl522

Alternate gene names: 50365339

Gene position: 615162-613864 (Counterclockwise)

Preceding gene: 50365340

Following gene: 50365338

Centisome position: 77.55

GC content: 30.64

Gene sequence:

>1299_bases
ATGAAATTTATTGATACAGCTAAGTTTACTATTAAAGCCGGTAATGGAGGGAATGGAGCAGTTAGTTTTCATACTGCGCT
TTTCGTACCTAATGGTGGTCCAAACGGTGGAGATGGTGGAAATGGTGGAAGTGTAATATTTGAAGCTGATGGTGGAAAAC
ATTCTCTTTTAGATTTAAAACTTCAAAAACAACTTAGTGCACAAGATGGATTTAAAGGTGATATAAAAAATATGCATGGT
GCTCAAGGTAAAGATCTTATCGTTCGAGTTCCCGTGGGTACATTGATTATAGAAAATAAAACTGGAACAATTTTAGCTGA
CATGGATGAAGATAAAAAACAAGTCTTAGTTGCTAAAGGTGGAAAAGGTGGAAAAGGGAACGCAAGATTTGCAAACTCAA
GAAATAAAGCTCCAACCATATTTGAAGCTGGTGAAATAGGACAATTTTATGAAGTTAAAGCTGAATTAAAAGTTTTAGCT
GATGTAGGTTTTGTTGGATTGCCCAATGCTGGTAAATCAACTTTACTTAGAGCAATTTCAAATTCAAAACCTGAAGTTGC
TGACTATGCTTTTACAACTTTAAATCCTCAATTAGGAGTTTCAAGAGCTAAAGATGGTTCAACATTTGTTGTTGCAGATT
TACCAGGTTTAATTGAAGGAGCTAGTTTAGGAAAAGGATTAGGTCATCAATTTTTAAAACATATTGAAAGATGTAGAGTT
ATATGCCATGTTTTAGATATGAGTGGAAATTATGGTCAAGAAGATGTAATCAAAAACTATGAATTAATAAGAAGTGAATT
AGTAAAATATAATTATAAACTGGATGAAAGACCAGAAATAATTGTTGCTAATAAAATGGATACAGATGAAGCACAATTAA
ATATGATGGAAGAAGACATAAAAAAATATTTCAAAGATAAAAAAGTTGTTTTTGTATCAGGATTATTAAAAGATAATGTT
GATGAATTATTATTAAAAATCGCAAAAGAACTTGAAACTGCTAAATATGTTCCACTATGAGAAATGGAACAAGATATTTA
TGAAGGTATCAAAGTTTATAGATTAGAAGAAGACGAAGAAGATATTCAAATAATTAATAAAGGTAATGGTAGATGAGAAG
TTGCCGGAGATACAATTTACAAAATTTATCAAAAGTTCCCAATTACAACAGATGATAACTTATTATTATTTAATGAAAAA
CTTAAAAAATCTGGTGTTTATGACATGTTAAGAGAACGTGGAGTTGGAGCTGGAGATATTGTAAAAGTATTTGAATATGA
ACTTGAGTGAATGGATTAA

Upstream 100 bases:

>100_bases
TTCGAGGATATTAATTTTGATAATCAAGAACCAACATTCTTATGTGATGAATGTTGAAATCAAATGATTTGATAAAGTAG
AAAGAAAGGAGCTGGAAAAT

Downstream 100 bases:

>100_bases
TTTGTAAAGGAGTAATATTATGGAATTAAAAGAATATTTAGATTATTTAGTTGAATTTATTAAAGAAACAGTTAAAAAAG
CTAATGCAAAAGGTGTAGTT

Product: GTPase ObgE

Products: NA

Alternate protein names: GTP-binding protein obg [H]

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MKFIDTAKFTIKAGNGGNGAVSFHTALFVPNGGPNGGDGGNGGSVIFEADGGKHSLLDLKLQKQLSAQDGFKGDIKNMHG
AQGKDLIVRVPVGTLIIENKTGTILADMDEDKKQVLVAKGGKGGKGNARFANSRNKAPTIFEAGEIGQFYEVKAELKVLA
DVGFVGLPNAGKSTLLRAISNSKPEVADYAFTTLNPQLGVSRAKDGSTFVVADLPGLIEGASLGKGLGHQFLKHIERCRV
ICHVLDMSGNYGQEDVIKNYELIRSELVKYNYKLDERPEIIVANKMDTDEAQLNMMEEDIKKYFKDKKVVFVSGLLKDNV
DELLLKIAKELETAKYVPLWEMEQDIYEGIKVYRLEEDEEDIQIINKGNGRWEVAGDTIYKIYQKFPITTDDNLLLFNEK
LKKSGVYDMLRERGVGAGDIVKVFEYELEWMD

Sequences:

>Translated_432_residues
MKFIDTAKFTIKAGNGGNGAVSFHTALFVPNGGPNGGDGGNGGSVIFEADGGKHSLLDLKLQKQLSAQDGFKGDIKNMHG
AQGKDLIVRVPVGTLIIENKTGTILADMDEDKKQVLVAKGGKGGKGNARFANSRNKAPTIFEAGEIGQFYEVKAELKVLA
DVGFVGLPNAGKSTLLRAISNSKPEVADYAFTTLNPQLGVSRAKDGSTFVVADLPGLIEGASLGKGLGHQFLKHIERCRV
ICHVLDMSGNYGQEDVIKNYELIRSELVKYNYKLDERPEIIVANKMDTDEAQLNMMEEDIKKYFKDKKVVFVSGLLKDNV
DELLLKIAKELETAKYVPL*EMEQDIYEGIKVYRLEEDEEDIQIINKGNGR*EVAGDTIYKIYQKFPITTDDNLLLFNEK
LKKSGVYDMLRERGVGAGDIVKVFEYELE*MD
>Mature_432_residues
MKFIDTAKFTIKAGNGGNGAVSFHTALFVPNGGPNGGDGGNGGSVIFEADGGKHSLLDLKLQKQLSAQDGFKGDIKNMHG
AQGKDLIVRVPVGTLIIENKTGTILADMDEDKKQVLVAKGGKGGKGNARFANSRNKAPTIFEAGEIGQFYEVKAELKVLA
DVGFVGLPNAGKSTLLRAISNSKPEVADYAFTTLNPQLGVSRAKDGSTFVVADLPGLIEGASLGKGLGHQFLKHIERCRV
ICHVLDMSGNYGQEDVIKNYELIRSELVKYNYKLDERPEIIVANKMDTDEAQLNMMEEDIKKYFKDKKVVFVSGLLKDNV
DELLLKIAKELETAKYVPL*EMEQDIYEGIKVYRLEEDEEDIQIINKGNGR*EVAGDTIYKIYQKFPITTDDNLLLFNEK
LKKSGVYDMLRERGVGAGDIVKVFEYELE*MD

Specific function: An essential GTPase which binds GTP, GDP and possibly (p)ppGpp with moderate affinity, with high nucleotide exchange rates and a fairly low GTP hydrolysis rate. It may play a role in control of the cell cycle, stress response, ribosome biogenesis and in t

COG id: COG0536

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI24308117, Length=328, Percent_Identity=42.0731707317073, Blast_Score=190, Evalue=2e-48,
Organism=Homo sapiens, GI111955139, Length=236, Percent_Identity=40.6779661016949, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI111955063, Length=187, Percent_Identity=40.1069518716578, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI4758796, Length=128, Percent_Identity=41.40625, Blast_Score=82, Evalue=9e-16,
Organism=Homo sapiens, GI58761500, Length=128, Percent_Identity=32.8125, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI4557537, Length=99, Percent_Identity=38.3838383838384, Blast_Score=66, Evalue=6e-11,
Organism=Escherichia coli, GI1789574, Length=287, Percent_Identity=49.1289198606272, Blast_Score=242, Evalue=4e-65,
Organism=Escherichia coli, GI1787454, Length=138, Percent_Identity=33.3333333333333, Blast_Score=71, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17508313, Length=322, Percent_Identity=39.1304347826087, Blast_Score=167, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17552324, Length=306, Percent_Identity=32.0261437908497, Blast_Score=141, Evalue=6e-34,
Organism=Caenorhabditis elegans, GI17555344, Length=119, Percent_Identity=40.3361344537815, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71981008, Length=119, Percent_Identity=37.8151260504202, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17509631, Length=139, Percent_Identity=30.2158273381295, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6321962, Length=183, Percent_Identity=38.7978142076503, Blast_Score=114, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6319281, Length=127, Percent_Identity=36.2204724409449, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6321612, Length=168, Percent_Identity=32.1428571428571, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI20129375, Length=338, Percent_Identity=38.4615384615385, Blast_Score=184, Evalue=1e-46,
Organism=Drosophila melanogaster, GI24585318, Length=298, Percent_Identity=36.5771812080537, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI17981711, Length=256, Percent_Identity=32.8125, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21356473, Length=127, Percent_Identity=34.6456692913386, Blast_Score=66, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014100
- InterPro:   IPR015349
- InterPro:   IPR006074
- InterPro:   IPR006073
- InterPro:   IPR006169
- InterPro:   IPR002917
- InterPro:   IPR005225 [H]

Pfam domain/function: PF09269 DUF1967; PF01018 GTP1_OBG; PF01926 MMR_HSR1 [H]

EC number: NA

Molecular weight: Translated: 47086; Mature: 47086

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS00905 GTP1_OBG

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFIDTAKFTIKAGNGGNGAVSFHTALFVPNGGPNGGDGGNGGSVIFEADGGKHSLLDLK
CCCEECEEEEEEECCCCCCEEEEEEEEEEECCCCCCCCCCCCCEEEEECCCCCCEEEEHH
LQKQLSAQDGFKGDIKNMHGAQGKDLIVRVPVGTLIIENKTGTILADMDEDKKQVLVAKG
HHHHHHHCCCCCCCHHHCCCCCCCCEEEEECCCEEEEECCCCEEEEECCCCCEEEEEEEC
GKGGKGNARFANSRNKAPTIFEAGEIGQFYEVKAELKVLADVGFVGLPNAGKSTLLRAIS
CCCCCCCCCCCCCCCCCCCEEECCCCCCCEEHHHHHHEEECCCEEECCCCCHHHHHHHHH
NSKPEVADYAFTTLNPQLGVSRAKDGSTFVVADLPGLIEGASLGKGLGHQFLKHIERCRV
CCCCCHHHEEEEECCCCCCCCCCCCCCEEEEECCCCHHCCHHHCCHHHHHHHHHHHHHHH
ICHVLDMSGNYGQEDVIKNYELIRSELVKYNYKLDERPEIIVANKMDTDEAQLNMMEEDI
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHH
KKYFKDKKVVFVSGLLKDNVDELLLKIAKELETAKYVPLEMEQDIYEGIKVYRLEEDEED
HHHHCCCCEEEEECHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHCCEEEEEECCCCCC
IQIINKGNGREVAGDTIYKIYQKFPITTDDNLLLFNEKLKKSGVYDMLRERGVGAGDIVK
EEEEECCCCCEECHHHHHHHHHHCCCCCCCCEEEEEHHHHHCCHHHHHHHCCCCCHHHEE
VFEYELEMD
EEEEEECCC
>Mature Secondary Structure
MKFIDTAKFTIKAGNGGNGAVSFHTALFVPNGGPNGGDGGNGGSVIFEADGGKHSLLDLK
CCCEECEEEEEEECCCCCCEEEEEEEEEEECCCCCCCCCCCCCEEEEECCCCCCEEEEHH
LQKQLSAQDGFKGDIKNMHGAQGKDLIVRVPVGTLIIENKTGTILADMDEDKKQVLVAKG
HHHHHHHCCCCCCCHHHCCCCCCCCEEEEECCCEEEEECCCCEEEEECCCCCEEEEEEEC
GKGGKGNARFANSRNKAPTIFEAGEIGQFYEVKAELKVLADVGFVGLPNAGKSTLLRAIS
CCCCCCCCCCCCCCCCCCCEEECCCCCCCEEHHHHHHEEECCCEEECCCCCHHHHHHHHH
NSKPEVADYAFTTLNPQLGVSRAKDGSTFVVADLPGLIEGASLGKGLGHQFLKHIERCRV
CCCCCHHHEEEEECCCCCCCCCCCCCCEEEEECCCCHHCCHHHCCHHHHHHHHHHHHHHH
ICHVLDMSGNYGQEDVIKNYELIRSELVKYNYKLDERPEIIVANKMDTDEAQLNMMEEDI
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHH
KKYFKDKKVVFVSGLLKDNVDELLLKIAKELETAKYVPLEMEQDIYEGIKVYRLEEDEED
HHHHCCCCEEEEECHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHCCEEEEEECCCCCC
IQIINKGNGREVAGDTIYKIYQKFPITTDDNLLLFNEKLKKSGVYDMLRERGVGAGDIVK
EEEEECCCCCEECHHHHHHHHHHCCCCCCCCEEEEEHHHHHCCHHHHHHHCCCCCHHHEE
VFEYELEMD
EEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA