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The map label for this gene is nadE [H]

Identifier: 50365338

GI number: 50365338

Start: 613110

End: 613844

Strand: Reverse

Name: nadE [H]

Synonym: Mfl521

Alternate gene names: 50365338

Gene position: 613844-613110 (Counterclockwise)

Preceding gene: 50365339

Following gene: 50365337

Centisome position: 77.39

GC content: 30.34

Gene sequence:

>735_bases
ATGGAATTAAAAGAATATTTAGATTATTTAGTTGAATTTATTAAAGAAACAGTTAAAAAAGCTAATGCAAAAGGTGTAGT
TATTGGAATAAGTGGTGGAATTGATTCTGCAGTTGTTGCTTGTTTAGCTAAAAAGGCCTTTCCTAACGATTACACTGCTG
TTTGAATGCCGATTGAATCAAGTGATGAAGATTACAAATGTAAACAAGAGTTGATTGATCAATGTGGTATTAAAGCAATT
GATGTAGAATTAAAAGAAACTTTTTTATCTTTTAAAAAAGCTATTAAAGATTCAACAACACCTGAACATAAATTGGCTAT
TGCAAATGCAAAAGCACGTTTAAGAATGACAACACTTTATACTGTTGCTCAAACAAATTCATATTTAGTTCTGGGAACTG
ACAATCTTGATGAGTGACATATTGGTTACTTTACTAAATTCGGTGATGGTGGAGTTGATATGGTTCCATTAGTACATTTA
TTAAAAAGAGAAGTTAGAGAAGCAGCGAGAATATTAGGCGTACCAACTTCAATTATAAATCGTGCACCAACAGCAAGTTT
ATGAGAAGACCAAACTGATGAGAGTGAGTTAGGTATTACTTATGATCAAATAGATGCTTATTTAGCTGGTGAAATTAATG
ATGAAAATGTAAAATCAAGAGTTGATCATTTACATAAAATTAGTGAACATAAAAGAAATGGTGCTGTTGCACCAAAAGAA
TTTAAAAGAAAATAA

Upstream 100 bases:

>100_bases
TATGACATGTTAAGAGAACGTGGAGTTGGAGCTGGAGATATTGTAAAAGTATTTGAATATGAACTTGAGTGAATGGATTA
ATTTGTAAAGGAGTAATATT

Downstream 100 bases:

>100_bases
ATACAAGGAGAAATTAACATGAAAAAAGTAATTATGAGAGGTGCTGAAATTTTATTTACAGCCGAATCTGACAATCAACA
AGTTTTAGATCAAATGCTTT

Product: NH3-dependent NAD+ synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MELKEYLDYLVEFIKETVKKANAKGVVIGISGGIDSAVVACLAKKAFPNDYTAVWMPIESSDEDYKCKQELIDQCGIKAI
DVELKETFLSFKKAIKDSTTPEHKLAIANAKARLRMTTLYTVAQTNSYLVLGTDNLDEWHIGYFTKFGDGGVDMVPLVHL
LKREVREAARILGVPTSIINRAPTASLWEDQTDESELGITYDQIDAYLAGEINDENVKSRVDHLHKISEHKRNGAVAPKE
FKRK

Sequences:

>Translated_244_residues
MELKEYLDYLVEFIKETVKKANAKGVVIGISGGIDSAVVACLAKKAFPNDYTAV*MPIESSDEDYKCKQELIDQCGIKAI
DVELKETFLSFKKAIKDSTTPEHKLAIANAKARLRMTTLYTVAQTNSYLVLGTDNLDE*HIGYFTKFGDGGVDMVPLVHL
LKREVREAARILGVPTSIINRAPTASL*EDQTDESELGITYDQIDAYLAGEINDENVKSRVDHLHKISEHKRNGAVAPKE
FKRK
>Mature_244_residues
MELKEYLDYLVEFIKETVKKANAKGVVIGISGGIDSAVVACLAKKAFPNDYTAV*MPIESSDEDYKCKQELIDQCGIKAI
DVELKETFLSFKKAIKDSTTPEHKLAIANAKARLRMTTLYTVAQTNSYLVLGTDNLDE*HIGYFTKFGDGGVDMVPLVHL
LKREVREAARILGVPTSIINRAPTASL*EDQTDESELGITYDQIDAYLAGEINDENVKSRVDHLHKISEHKRNGAVAPKE
FKRK

Specific function: This NAD Synthase Uses Nh(3) In Preference To Glutamine. [C]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1788036, Length=244, Percent_Identity=31.5573770491803, Blast_Score=115, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17538756, Length=184, Percent_Identity=31.5217391304348, Blast_Score=70, Evalue=8e-13,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR022926
- InterPro:   IPR014729 [H]

Pfam domain/function: PF02540 NAD_synthase [H]

EC number: =6.3.1.5 [H]

Molecular weight: Translated: 26725; Mature: 26725

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELKEYLDYLVEFIKETVKKANAKGVVIGISGGIDSAVVACLAKKAFPNDYTAVMPIESS
CCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCEEEEECCCC
DEDYKCKQELIDQCGIKAIDVELKETFLSFKKAIKDSTTPEHKLAIANAKARLRMTTLYT
CCCHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHEEHHHEEE
VAQTNSYLVLGTDNLDEHIGYFTKFGDGGVDMVPLVHLLKREVREAARILGVPTSIINRA
EECCCCEEEEECCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCC
PTASLEDQTDESELGITYDQIDAYLAGEINDENVKSRVDHLHKISEHKRNGAVAPKEFKR
CCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHCC
K
C
>Mature Secondary Structure
MELKEYLDYLVEFIKETVKKANAKGVVIGISGGIDSAVVACLAKKAFPNDYTAVMPIESS
CCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCEEEEECCCC
DEDYKCKQELIDQCGIKAIDVELKETFLSFKKAIKDSTTPEHKLAIANAKARLRMTTLYT
CCCHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHEEHHHEEE
VAQTNSYLVLGTDNLDEHIGYFTKFGDGGVDMVPLVHLLKREVREAARILGVPTSIINRA
EECCCCEEEEECCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCC
PTASLEDQTDESELGITYDQIDAYLAGEINDENVKSRVDHLHKISEHKRNGAVAPKEFKR
CCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHCC
K
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA