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The map label for this gene is treB [H]

Identifier: 50365317

GI number: 50365317

Start: 586503

End: 588062

Strand: Reverse

Name: treB [H]

Synonym: Mfl500

Alternate gene names: 50365317

Gene position: 588062-586503 (Counterclockwise)

Preceding gene: 50365318

Following gene: 50365316

Centisome position: 74.14

GC content: 31.99

Gene sequence:

>1560_bases
ATGGGTAAAAAAGAAAAATTACCACAAGTAAAGATTGATTTAAAAGCTTGATCAAAAGAACTTGTTGAATATCTTGGTGG
TTCTGAAAATATTATTTCAGCAACTCACTGTTTGACAAGACTAAGACTTGTTATTAAAGATGAAAATCTAATTAATAAAG
CAAAAGTTGAAACAATGCCAAGTGTTAAAGGTACTTTTAAATCTTCTGGTCAATATCAAATTATCATTGGAACAGAAGTT
GAAAAATACTTTAAAGAGTTTGTAGCTGTTTCAGGGGTTGAAGCTGTTTCTAAGGAAAAAGCTAAAGCCATCGCAAACCA
AAATAATGGTGGGTGATTCTTAAAATCACTTAACTTTTTAGGAGAAGTATTTATTCCAATTATTCCTGTTTTAGTAGCAG
GTGGGATAATCCTAGGATTTAGAAATATTTTAGAAGCAGATTTTAATGGCTTTATAATTGTTAAATCTGGTCAATTCTGA
CAAGGACTTGATGACTTCTTATGAATTCCAGCTCAAGTTTGTTTCTGATGACTTCCAGTTCATTTATGCTGAAGTATTTT
TAGAAAAATGGAAGCTGACCAAGTTATGGGAATTGTTGTTGGACTATGTTTATTAGTTCCACCTTTAATGAACGTTTATG
AAGTTTCTGGTGAAGCTTCACAAGATGGTGGATTTAAATGAATCTGACAAATTATGGCAGGTATGGATGATGGAGCCTTT
GACTGAGGTTTCATGAAATATCCTTGAAAAATTCAATATACAGCGCAAGTTATTCCTGCGTTTGCTATTGGTATTGTTGG
TGCTTATATCAATAAATGAATTAAAAGAACATCACCAGCAGTTGTAAGTCAAATTGTTGTTCCGTTAGTAACAATTTTAC
CAACATTTACATTAGCTATGTTTGTAATTGGTCCTGCAGGATTTATAGTTGCATCAGTTATCAGTTTTGCTATTTCATGA
GCATTTACAAATAACATTGCAAAATACTTCTTCGGATTTATAATTGGTATGGCGTATGCACCAATTGTATTAACAGGAGT
TCACCACTTATTTAATGCAGTGTTTGTACAAGACACAATACAAAATGGAGGAAACTTCTTATTTATTGGAACATGTGCTC
AAGCTATTGCTCAAGGAAGTGCTGTTCTTGGTTGAATATTAGTTAACAAAAAAGATCCAAGAGCAAAAGATGTAGGTATT
CCATCAGTTGTTTCAGCTTATCTAGGAGTTACTGAACCAGCAATGTATGGTGTTAACTTAAAACATATGTATCCATTTGT
AGCAGCTTCAATTGCAACAGGTTTAGGATTAGAATTAGCAGTTATTTCAGGTGTTAGTGCAACTAACTCAGGAAATGGTG
CGTGATTAGGAATTTTAAATGTTCAAGTTGAATCAAAAATTGAAGGTGTTAATACTTGAATTGGAACTGGTTATACATGA
TTTATGATATCAATGATATTAACAGCAGCAGTTTCAATAGGATTAACAATGGTATTCTCAAAAGTTAAATATTTCGAAAA
ATTCAATATCGAAGTAAAAGCAGCAGAATTACTAAAATAA

Upstream 100 bases:

>100_bases
AAGAAAAGAAAATGGAAAATTTCTTCAAAATTATTTAATAGTTTATTTTTTTATATATAATAATAGTGTTGGAAATGTTT
CCAATAGAAAGGCAAAGATT

Downstream 100 bases:

>100_bases
TATTAAAATAATATTAAAATAAAACTAAATAGAAAAAGGGAAAAAATGAGAAATGAAGTAATTTATCAGATATTCCCTTT
AACTTTTTCTGATGGTAAGA

Product: trehalose PTS system IIABC component

Products: NA

Alternate protein names: EIIBC-Tre; EII-Tre; Trehalose-specific phosphotransferase enzyme IIB component; PTS system trehalose-specific EIIB component; Trehalose permease IIC component; PTS system trehalose-specific EIIC component [H]

Number of amino acids: Translated: 519; Mature: 518

Protein sequence:

>519_residues
MGKKEKLPQVKIDLKAWSKELVEYLGGSENIISATHCLTRLRLVIKDENLINKAKVETMPSVKGTFKSSGQYQIIIGTEV
EKYFKEFVAVSGVEAVSKEKAKAIANQNNGGWFLKSLNFLGEVFIPIIPVLVAGGIILGFRNILEADFNGFIIVKSGQFW
QGLDDFLWIPAQVCFWWLPVHLCWSIFRKMEADQVMGIVVGLCLLVPPLMNVYEVSGEASQDGGFKWIWQIMAGMDDGAF
DWGFMKYPWKIQYTAQVIPAFAIGIVGAYINKWIKRTSPAVVSQIVVPLVTILPTFTLAMFVIGPAGFIVASVISFAISW
AFTNNIAKYFFGFIIGMAYAPIVLTGVHHLFNAVFVQDTIQNGGNFLFIGTCAQAIAQGSAVLGWILVNKKDPRAKDVGI
PSVVSAYLGVTEPAMYGVNLKHMYPFVAASIATGLGLELAVISGVSATNSGNGAWLGILNVQVESKIEGVNTWIGTGYTW
FMISMILTAAVSIGLTMVFSKVKYFEKFNIEVKAAELLK

Sequences:

>Translated_519_residues
MGKKEKLPQVKIDLKA*SKELVEYLGGSENIISATHCLTRLRLVIKDENLINKAKVETMPSVKGTFKSSGQYQIIIGTEV
EKYFKEFVAVSGVEAVSKEKAKAIANQNNGG*FLKSLNFLGEVFIPIIPVLVAGGIILGFRNILEADFNGFIIVKSGQF*
QGLDDFL*IPAQVCF**LPVHLC*SIFRKMEADQVMGIVVGLCLLVPPLMNVYEVSGEASQDGGFK*I*QIMAGMDDGAF
D*GFMKYP*KIQYTAQVIPAFAIGIVGAYINK*IKRTSPAVVSQIVVPLVTILPTFTLAMFVIGPAGFIVASVISFAIS*
AFTNNIAKYFFGFIIGMAYAPIVLTGVHHLFNAVFVQDTIQNGGNFLFIGTCAQAIAQGSAVLG*ILVNKKDPRAKDVGI
PSVVSAYLGVTEPAMYGVNLKHMYPFVAASIATGLGLELAVISGVSATNSGNGA*LGILNVQVESKIEGVNT*IGTGYT*
FMISMILTAAVSIGLTMVFSKVKYFEKFNIEVKAAELLK
>Mature_518_residues
GKKEKLPQVKIDLKA*SKELVEYLGGSENIISATHCLTRLRLVIKDENLINKAKVETMPSVKGTFKSSGQYQIIIGTEVE
KYFKEFVAVSGVEAVSKEKAKAIANQNNGG*FLKSLNFLGEVFIPIIPVLVAGGIILGFRNILEADFNGFIIVKSGQF*Q
GLDDFL*IPAQVCF**LPVHLC*SIFRKMEADQVMGIVVGLCLLVPPLMNVYEVSGEASQDGGFK*I*QIMAGMDDGAFD
*GFMKYP*KIQYTAQVIPAFAIGIVGAYINK*IKRTSPAVVSQIVVPLVTILPTFTLAMFVIGPAGFIVASVISFAIS*A
FTNNIAKYFFGFIIGMAYAPIVLTGVHHLFNAVFVQDTIQNGGNFLFIGTCAQAIAQGSAVLG*ILVNKKDPRAKDVGIP
SVVSAYLGVTEPAMYGVNLKHMYPFVAASIATGLGLELAVISGVSATNSGNGA*LGILNVQVESKIEGVNT*IGTGYT*F
MISMILTAAVSIGLTMVFSKVKYFEKFNIEVKAAELLK

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1263

COG function: function code G; Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI2367362, Length=461, Percent_Identity=34.4902386117137, Blast_Score=253, Evalue=3e-68,
Organism=Escherichia coli, GI48994906, Length=498, Percent_Identity=26.1044176706827, Blast_Score=132, Evalue=5e-32,
Organism=Escherichia coli, GI1790159, Length=415, Percent_Identity=24.8192771084337, Blast_Score=118, Evalue=8e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018113
- InterPro:   IPR004719
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013
- InterPro:   IPR011535
- InterPro:   IPR011296 [H]

Pfam domain/function: PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 53428; Mature: 53297

Theoretical pI: Translated: 8.81; Mature: 8.81

Prosite motif: PS01035 PTS_EIIB_TYPE_1_CYS ; PS51098 PTS_EIIB_TYPE_1 ; PS51103 PTS_EIIC_TYPE_1 ; PS00134 TRYPSIN_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKKEKLPQVKIDLKASKELVEYLGGSENIISATHCLTRLRLVIKDENLINKAKVETMPS
CCCCCCCCCEEEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCC
VKGTFKSSGQYQIIIGTEVEKYFKEFVAVSGVEAVSKEKAKAIANQNNGGFLKSLNFLGE
CCCCCCCCCCEEEEECCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
VFIPIIPVLVAGGIILGFRNILEADFNGFIIVKSGQFQGLDDFLIPAQVCFLPVHLCSIF
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHH
RKMEADQVMGIVVGLCLLVPPLMNVYEVSGEASQDGGFKIQIMAGMDDGAFDGFMKYPKI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCHHHHCCCCH
QYTAQVIPAFAIGIVGAYINKIKRTSPAVVSQIVVPLVTILPTFTLAMFVIGPAGFIVAS
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
VISFAISAFTNNIAKYFFGFIIGMAYAPIVLTGVHHLFNAVFVQDTIQNGGNFLFIGTCA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHH
QAIAQGSAVLGILVNKKDPRAKDVGIPSVVSAYLGVTEPAMYGVNLKHMYPFVAASIATG
HHHHCCCEEEEEEEECCCCCCCCCCCHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHHHHC
LGLELAVISGVSATNSGNGALGILNVQVESKIEGVNTIGTGYTFMISMILTAAVSIGLTM
CCEEEEEEECCCCCCCCCCEEEEEEEEEHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
VFSKVKYFEKFNIEVKAAELLK
HHHHHHHHHHCCCEEEHHHHCC
>Mature Secondary Structure 
GKKEKLPQVKIDLKASKELVEYLGGSENIISATHCLTRLRLVIKDENLINKAKVETMPS
CCCCCCCCEEEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCC
VKGTFKSSGQYQIIIGTEVEKYFKEFVAVSGVEAVSKEKAKAIANQNNGGFLKSLNFLGE
CCCCCCCCCCEEEEECCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
VFIPIIPVLVAGGIILGFRNILEADFNGFIIVKSGQFQGLDDFLIPAQVCFLPVHLCSIF
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHH
RKMEADQVMGIVVGLCLLVPPLMNVYEVSGEASQDGGFKIQIMAGMDDGAFDGFMKYPKI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCHHHHCCCCH
QYTAQVIPAFAIGIVGAYINKIKRTSPAVVSQIVVPLVTILPTFTLAMFVIGPAGFIVAS
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
VISFAISAFTNNIAKYFFGFIIGMAYAPIVLTGVHHLFNAVFVQDTIQNGGNFLFIGTCA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHH
QAIAQGSAVLGILVNKKDPRAKDVGIPSVVSAYLGVTEPAMYGVNLKHMYPFVAASIATG
HHHHCCCEEEEEEEECCCCCCCCCCCHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHHHHC
LGLELAVISGVSATNSGNGALGILNVQVESKIEGVNTIGTGYTFMISMILTAAVSIGLTM
CCEEEEEEECCCCCCCCCCEEEEEEEEEHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
VFSKVKYFEKFNIEVKAAELLK
HHHHHHHHHHCCCEEEHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7608078; 7610040; 9278503 [H]