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The map label for this gene is grpE

Identifier: 50365234

GI number: 50365234

Start: 487188

End: 487751

Strand: Reverse

Name: grpE

Synonym: Mfl416

Alternate gene names: 50365234

Gene position: 487751-487188 (Counterclockwise)

Preceding gene: 50365235

Following gene: 50365233

Centisome position: 61.49

GC content: 27.66

Gene sequence:

>564_bases
ATGAATAACGAAAAAGAATTAAAAAAAGAAGAAACTTCAGTTGAGAATAAAGAGAAAAAAGTTGCAACTGAAGAAATCAA
AAAAGAGAAAAAAGATTATAAAAAAATAATTCAAGATCTTGAAAAGCAAATTGAGAGTTGTCAAAAAGAAATTGAATTTC
AAAAAAGTTTAAGAAATGCTGATATTGCAAATCTTACAAAAAAAAGAAATGAACAAGAAGCTTTAGTTAGAAAATATGGT
TCAAGCAATTTAGCTGAGGACTTAATAAAACCAATTGACTTACTAAAAAAAGTGGTTGAGACACCTACAGATATTCCAGA
GTTACAAAATTATTTGATGGGATTTAAAATGATCATTAGTCAAATTGAAAATGCATTTGAAACTAACGGAATAAAAGCAA
TGGGTGTAAAAGCGGGGGATGAATTTGATTCAAGCTTTCATGAAGCAAATGAATCACTGGAAAACAGTGGAATGGAATCA
AATAAAATAGTATCTGTAATATCAGATGGATATATGATTCATGATAGAGTATTAATTCACGCAATTGTAAAAGTAGCAAA
ATAG

Upstream 100 bases:

>100_bases
ACAATGTTAACTTTGGTTGGGCCTAAAAGAGTTGATTATTCTCAAGCTAATCAATTAATTAACTTGATAGTAGAAATAAT
AAATGGAGATGATCATAAAA

Downstream 100 bases:

>100_bases
TATTAAGAGGAGAAATAAATTATGGCAAAAGAAAGAATTATAGGAATAGACTTAGGTACAACTAACTCAGTTGTTTCAAT
TATGGAAGGTGGGCAACCAA

Product: hsp70 cofactor

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 187; Mature: 187

Protein sequence:

>187_residues
MNNEKELKKEETSVENKEKKVATEEIKKEKKDYKKIIQDLEKQIESCQKEIEFQKSLRNADIANLTKKRNEQEALVRKYG
SSNLAEDLIKPIDLLKKVVETPTDIPELQNYLMGFKMIISQIENAFETNGIKAMGVKAGDEFDSSFHEANESLENSGMES
NKIVSVISDGYMIHDRVLIHAIVKVAK

Sequences:

>Translated_187_residues
MNNEKELKKEETSVENKEKKVATEEIKKEKKDYKKIIQDLEKQIESCQKEIEFQKSLRNADIANLTKKRNEQEALVRKYG
SSNLAEDLIKPIDLLKKVVETPTDIPELQNYLMGFKMIISQIENAFETNGIKAMGVKAGDEFDSSFHEANESLENSGMES
NKIVSVISDGYMIHDRVLIHAIVKVAK
>Mature_187_residues
MNNEKELKKEETSVENKEKKVATEEIKKEKKDYKKIIQDLEKQIESCQKEIEFQKSLRNADIANLTKKRNEQEALVRKYG
SSNLAEDLIKPIDLLKKVVETPTDIPELQNYLMGFKMIISQIENAFETNGIKAMGVKAGDEFDSSFHEANESLENSGMES
NKIVSVISDGYMIHDRVLIHAIVKVAK

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GRPE_MESFL (Q6F148)

Other databases:

- EMBL:   AE017263
- RefSeq:   YP_053659.1
- ProteinModelPortal:   Q6F148
- GeneID:   2897591
- GenomeReviews:   AE017263_GR
- KEGG:   mfl:Mfl416
- HOGENOM:   HBG129479
- OMA:   IDQFEIA
- ProtClustDB:   CLSK745849
- BioCyc:   MFLO265311:MFL416-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 21363; Mature: 21363

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNEKELKKEETSVENKEKKVATEEIKKEKKDYKKIIQDLEKQIESCQKEIEFQKSLRNA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DIANLTKKRNEQEALVRKYGSSNLAEDLIKPIDLLKKVVETPTDIPELQNYLMGFKMIIS
HHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
QIENAFETNGIKAMGVKAGDEFDSSFHEANESLENSGMESNKIVSVISDGYMIHDRVLIH
HHHHHHHHCCCCEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHH
AIVKVAK
HHHHHCC
>Mature Secondary Structure
MNNEKELKKEETSVENKEKKVATEEIKKEKKDYKKIIQDLEKQIESCQKEIEFQKSLRNA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DIANLTKKRNEQEALVRKYGSSNLAEDLIKPIDLLKKVVETPTDIPELQNYLMGFKMIIS
HHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
QIENAFETNGIKAMGVKAGDEFDSSFHEANESLENSGMESNKIVSVISDGYMIHDRVLIH
HHHHHHHHCCCCEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHH
AIVKVAK
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA