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The map label for this gene is pheS [H]

Identifier: 50365217

GI number: 50365217

Start: 459100

End: 460152

Strand: Reverse

Name: pheS [H]

Synonym: Mfl399

Alternate gene names: 50365217

Gene position: 460152-459100 (Counterclockwise)

Preceding gene: 50365218

Following gene: 50365216

Centisome position: 58.01

GC content: 28.68

Gene sequence:

>1053_bases
ATGTTAAAAAAAATTAATAAGATTAAAGATCAATTTATATCTGATTTAAATAAAGTAAAAACAATTGAAGAAGCTGAAAA
ATTGAAAAAGTCTTTATTAGGTAAAGAATCAGAATTAAGTGAAATTTTGAAATCTTTAAAAGATTCTGATACTAATGATA
AACAAGCTATTGGAATTGCCAGTCATGAGTTAAGAACATTTATTGCATCAACAATTGATGAATTTGTAGCAAGAATTAAA
AAAGAAGCGCTTGATGCAAAATTAGCTAGTGAAAAAATTGACGTTTCACTAACAGGTACATTAGCAAGATTTGGAACTAA
ACACCCTCTTAATATTGTTGTTGAAGAGATTACACAAATCTTTACAGAAATTGGTTTTGATGTATTAAATGGAAATGATG
TTGAAAGCGATGAATATTGTTTTCAAAAATTAAACTTGCCAGTGGGGCACCCTGCTAGAGATATGCAAGACACTTTCTAT
ATAGATGAAGAAACTGTTTTAAGTACTCACTGTACACATATGACAGCTAGAGTTTTAACACAAATGGCTAAAGACGAAAA
CTTTGAAGGTAACTATGCCTGTGTTGCTATTGGTAATGTTTATCGAAGAGATGACGATGATGCAACTCATTCACACCAGT
TCATGCAATTAGATTTATTATGTATTGGTAAAAAAATTACTTTTGCTAACTTAAAATGAGTTTTAAAATACATGTGTAAA
AGATTATTTGGTGAAGAAGTTAATATTAGATTAAGACCAAGTCTATTCCCATTCACCGAACCAAGTGTAGAAGTTGATGT
AAGTTGTTTTAAATGTTCTGGTAAAGGTTGTTCAATTTGTAAATATTCAGGTTGAATTGAAATATTAGGATCAGGAATTA
TTAATGAACAAGTTATGCTATTAAATGGAATGGATCCAGAAAAAAATACTGCGTTAGCTTTTGGTGCAGGTATTGAAAGA
ATAGCAATGCTTAAATTTGGAATTTCAAACATTAGAAACTTATATGAAAATAATGTTCAATTTTTAGAACAATTTAAATT
TTATGGAGAGTAG

Upstream 100 bases:

>100_bases
TATAGTTATTCAAGCGTATGAAGAAAATTTAAAAGAAAATGAAATGAAGTTAATTGTTTTAAAACAAGAAGCTTTAATAA
AGTAGCAAAAGGAGTTTTTT

Downstream 100 bases:

>100_bases
GTAGAACATGATTATTACAAGAAAATGATTAGAAAAATACTTAGATCTTTCTAATATTACAAATGAACAAATTTCAGTTT
GTTTAAACTCTTTAGGATTT

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS [H]

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MLKKINKIKDQFISDLNKVKTIEEAEKLKKSLLGKESELSEILKSLKDSDTNDKQAIGIASHELRTFIASTIDEFVARIK
KEALDAKLASEKIDVSLTGTLARFGTKHPLNIVVEEITQIFTEIGFDVLNGNDVESDEYCFQKLNLPVGHPARDMQDTFY
IDEETVLSTHCTHMTARVLTQMAKDENFEGNYACVAIGNVYRRDDDDATHSHQFMQLDLLCIGKKITFANLKWVLKYMCK
RLFGEEVNIRLRPSLFPFTEPSVEVDVSCFKCSGKGCSICKYSGWIEILGSGIINEQVMLLNGMDPEKNTALAFGAGIER
IAMLKFGISNIRNLYENNVQFLEQFKFYGE

Sequences:

>Translated_350_residues
MLKKINKIKDQFISDLNKVKTIEEAEKLKKSLLGKESELSEILKSLKDSDTNDKQAIGIASHELRTFIASTIDEFVARIK
KEALDAKLASEKIDVSLTGTLARFGTKHPLNIVVEEITQIFTEIGFDVLNGNDVESDEYCFQKLNLPVGHPARDMQDTFY
IDEETVLSTHCTHMTARVLTQMAKDENFEGNYACVAIGNVYRRDDDDATHSHQFMQLDLLCIGKKITFANLK*VLKYMCK
RLFGEEVNIRLRPSLFPFTEPSVEVDVSCFKCSGKGCSICKYSG*IEILGSGIINEQVMLLNGMDPEKNTALAFGAGIER
IAMLKFGISNIRNLYENNVQFLEQFKFYGE
>Mature_350_residues
MLKKINKIKDQFISDLNKVKTIEEAEKLKKSLLGKESELSEILKSLKDSDTNDKQAIGIASHELRTFIASTIDEFVARIK
KEALDAKLASEKIDVSLTGTLARFGTKHPLNIVVEEITQIFTEIGFDVLNGNDVESDEYCFQKLNLPVGHPARDMQDTFY
IDEETVLSTHCTHMTARVLTQMAKDENFEGNYACVAIGNVYRRDDDDATHSHQFMQLDLLCIGKKITFANLK*VLKYMCK
RLFGEEVNIRLRPSLFPFTEPSVEVDVSCFKCSGKGCSICKYSG*IEILGSGIINEQVMLLNGMDPEKNTALAFGAGIER
IAMLKFGISNIRNLYENNVQFLEQFKFYGE

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758340, Length=280, Percent_Identity=29.6428571428571, Blast_Score=105, Evalue=5e-23,
Organism=Escherichia coli, GI1788007, Length=330, Percent_Identity=33.9393939393939, Blast_Score=206, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI17508957, Length=280, Percent_Identity=29.2857142857143, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI32563657, Length=280, Percent_Identity=29.2857142857143, Blast_Score=108, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6321087, Length=277, Percent_Identity=31.0469314079422, Blast_Score=109, Evalue=5e-25,
Organism=Drosophila melanogaster, GI18858079, Length=277, Percent_Identity=29.6028880866426, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI17137424, Length=307, Percent_Identity=26.3843648208469, Blast_Score=96, Evalue=3e-20,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978 [H]

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 39193; Mature: 39193

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKKINKIKDQFISDLNKVKTIEEAEKLKKSLLGKESELSEILKSLKDSDTNDKQAIGIA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCEECHH
SHELRTFIASTIDEFVARIKKEALDAKLASEKIDVSLTGTLARFGTKHPLNIVVEEITQI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEHHHHHCCCCCCHHHHHHHHHHH
FTEIGFDVLNGNDVESDEYCFQKLNLPVGHPARDMQDTFYIDEETVLSTHCTHMTARVLT
HHHHCCEEECCCCCCCHHHHHHHCCCCCCCCCCCCCHHEEECCHHHHHHHHHHHHHHHHH
QMAKDENFEGNYACVAIGNVYRRDDDDATHSHQFMQLDLLCIGKKITFANLKVLKYMCKR
HHHHCCCCCCCEEEEEECCHHCCCCCCCHHHHHHHEEEEEHCCCCCHHHHHHHHHHHHHH
LFGEEVNIRLRPSLFPFTEPSVEVDVSCFKCSGKGCSICKYSGIEILGSGIINEQVMLLN
HCCCCCCEEECCCCCCCCCCCCEEEEEEEEECCCCCCEEEECCHHHHCCCCCCCEEEEEE
GMDPEKNTALAFGAGIERIAMLKFGISNIRNLYENNVQFLEQFKFYGE
CCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLKKINKIKDQFISDLNKVKTIEEAEKLKKSLLGKESELSEILKSLKDSDTNDKQAIGIA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCEECHH
SHELRTFIASTIDEFVARIKKEALDAKLASEKIDVSLTGTLARFGTKHPLNIVVEEITQI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEHHHHHCCCCCCHHHHHHHHHHH
FTEIGFDVLNGNDVESDEYCFQKLNLPVGHPARDMQDTFYIDEETVLSTHCTHMTARVLT
HHHHCCEEECCCCCCCHHHHHHHCCCCCCCCCCCCCHHEEECCHHHHHHHHHHHHHHHHH
QMAKDENFEGNYACVAIGNVYRRDDDDATHSHQFMQLDLLCIGKKITFANLKVLKYMCKR
HHHHCCCCCCCEEEEEECCHHCCCCCCCHHHHHHHEEEEEHCCCCCHHHHHHHHHHHHHH
LFGEEVNIRLRPSLFPFTEPSVEVDVSCFKCSGKGCSICKYSGIEILGSGIINEQVMLLN
HCCCCCCEEECCCCCCCCCCCCEEEEEEEEECCCCCCEEEECCHHHHCCCCCCCEEEEEE
GMDPEKNTALAFGAGIERIAMLKFGISNIRNLYENNVQFLEQFKFYGE
CCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA