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The map label for this gene is pheT [H]

Identifier: 50365216

GI number: 50365216

Start: 456711

End: 459092

Strand: Reverse

Name: pheT [H]

Synonym: Mfl398

Alternate gene names: 50365216

Gene position: 459092-456711 (Counterclockwise)

Preceding gene: 50365217

Following gene: 50365215

Centisome position: 57.88

GC content: 23.93

Gene sequence:

>2382_bases
ATGATTATTACAAGAAAATGATTAGAAAAATACTTAGATCTTTCTAATATTACAAATGAACAAATTTCAGTTTGTTTAAA
CTCTTTAGGATTTGAAGTTGAACAAGAAATAGATTATTCAAAATTGAATACAAAATTAGTTATAGGACACATCGAAGAGT
CAGATCCAATTGAAGGAACAAAACTAAAGAAAACTAAAACTCGTATTGGTGAAAACAAATATGCAACAATTTTATCAACA
TCAAGAAACATTGAAGAAGATAAATTTGCAATAGTTGCTTTACCTGGGGCTAAATTAGCTAATGGTCTAGAATTAGAAAA
TAGAGAAATTTTAGGTATACTTTCAGAAGGAATGTTTTGTGGTTTTAATGAGATTGGATTACCTAATTCAGTTTTAACAG
AAGATGAACAACAAGATGTATACTACATTGATTCAATCTATAAAAACATGGATGAAATGGTTGGAAGAGAAATTAGTGAA
GTTTTAAATTTTGATGATTACACTTTTGAAGTAGATTTAACTTTAAATAGAAGTGATGCTTTAGCAGCAAAACAATTAGT
AAAAGAAATAGCTAACTATTTTGATTTAAAAATTAATAACAATGAACATAAAGTTAAATTTGAAAAACCAACTGAATCAA
TAACTATAGATTTAAATAAAAAAGTTGAAGATGAAGTAAATACTGTTTCACATACTTTTATTGGTTTAAAAAATACTAAT
ACACCTTTCGATTCATCTCATGACGTATGATTAAAACATTCAAATGTTAAATCAGCTCAAAACAAATTTGAAAATATAGC
TAATATGGCAACTTTAATTTCAGGTCAACCTTTTATTTTAATTGATGCTGATAAAGTTAATGGAGAATTAACTTTAACAA
AACAAACAATAGATGAAAAAGAATTAATTGTTTTAAAATCAGGCAACTCAATTGTTAATATTTTAGGACTAAAAACTGAA
GATAAATTTAAAGTTACTGAAGAAACAGTTACAATGCTTGTTGTAATGTTAAATTTAGACCAAATATCAATGCGTAAACA
ACAAAGAAACTTAAATGTTAGCACAGTTGATACTCAAAGATATGCAAAACCATTAAATCCAAACCTTTATGATCAAGGAA
TAGATGAATTAGTTTCAATATTAGATGAATATAAAATGTTAGAAGATGTTCAAAAAGTTGTAACAACAGTTCAAAAACAA
AATTATGACAATGTATATTCAATTACTTTAGAAAAAGTTCAAGATATTCTAGGTATTGAAATAACTGTTGAAGAAATAAT
AAGTTTATTTAGAACATTAGATATTGAAATATCTGTTAAAAAGTCTGAATTAACATTTACTGTTGATAAAAATAGAACTG
ATTTATATGGCAAGAATGATTTATGCGAAGAGATAGCTAGATTATATGGTTATGACAATATTATAGAACAACCTTTAGAA
TATGTAAGTTTCAAAAAAACTAAAAACTTAGATAAAAAACTTAAAGATAAATTAAGCGATTATTTAGTTGGTGCAGGTTT
TAACAATATTAAAACATATTCTTTAACTGATATAGAATCAAATAAAACTTGAAACTTATTTAAAGTTAAAGATCCTGTTG
TTTTAATGAGTCCTTTATCAATTCAACGTGAAACATTTAGAAATAATTTATCAAAATCTATGATAGATACAATTATTTTT
AATGCTAATAATGGAAACAAATCAGTAAAGTTTTTTGAAATGGCTGATATCTTTGCACTAAATGGATTCAGACAATCAAA
TTTATGTTTTGGTGTTTCAGGAGAAGCAATTACTGATTCATTAAGTCAAGTACATATTAAATCAAATTATGCTTATATAA
GTTCAATTTTAATGAGCGTTTTAGATTTATATAAAGTTGATGTAACAAATGTTACATTTGAAGTTAACGATAAAGCTATT
GATGAAATTCATCCTTATATTAATGCAACTGTAAAATATAAAAATAAAAAAATTGGATTTATATATAAATTAAATCCTGC
ATTTGAACAAGCTAATAAAATTTATCCTACATTCATTTGTGAAGTAAATTTAAACTTATTACTTGAGATTGCTGATAAAG
TGCATGTAACTAGTGAAATTTCTAAATTCCAAAAATCAACAAGAGATATTTCTTTTGAATTAAGTAATGATGTTAATTTT
GATACAATCGTTAAAGCAATGTTAAAAGATCTAAATTATTTAACAAATTACAAAGTAATAGACAAATATTCAGATGAACA
AATGGATAAAGAAAATATTTCATCATTAACTGTTAAATTATCATTTAATAGTTTAGATCACCAGTTAACTGAAAAAGAGA
TTAACGATGATTTTGAAAAAATATTAAATAATTTAAAAGAATTAAAAGTTAAAGTTAGATAG

Upstream 100 bases:

>100_bases
ATAGCAATGCTTAAATTTGGAATTTCAAACATTAGAAACTTATATGAAAATAATGTTCAATTTTTAGAACAATTTAAATT
TTATGGAGAGTAGGTAGAAC

Downstream 100 bases:

>100_bases
TACTATGAATAAACAATATTTTGAAAAACAAGTACATACAGAATTAACAGAAATAATAAATGATTTGGAATCAATAAAAC
TTAATAACATTTTAGTGAAA

Product: phenylalanyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase beta chain; PheRS [H]

Number of amino acids: Translated: 793; Mature: 793

Protein sequence:

>793_residues
MIITRKWLEKYLDLSNITNEQISVCLNSLGFEVEQEIDYSKLNTKLVIGHIEESDPIEGTKLKKTKTRIGENKYATILST
SRNIEEDKFAIVALPGAKLANGLELENREILGILSEGMFCGFNEIGLPNSVLTEDEQQDVYYIDSIYKNMDEMVGREISE
VLNFDDYTFEVDLTLNRSDALAAKQLVKEIANYFDLKINNNEHKVKFEKPTESITIDLNKKVEDEVNTVSHTFIGLKNTN
TPFDSSHDVWLKHSNVKSAQNKFENIANMATLISGQPFILIDADKVNGELTLTKQTIDEKELIVLKSGNSIVNILGLKTE
DKFKVTEETVTMLVVMLNLDQISMRKQQRNLNVSTVDTQRYAKPLNPNLYDQGIDELVSILDEYKMLEDVQKVVTTVQKQ
NYDNVYSITLEKVQDILGIEITVEEIISLFRTLDIEISVKKSELTFTVDKNRTDLYGKNDLCEEIARLYGYDNIIEQPLE
YVSFKKTKNLDKKLKDKLSDYLVGAGFNNIKTYSLTDIESNKTWNLFKVKDPVVLMSPLSIQRETFRNNLSKSMIDTIIF
NANNGNKSVKFFEMADIFALNGFRQSNLCFGVSGEAITDSLSQVHIKSNYAYISSILMSVLDLYKVDVTNVTFEVNDKAI
DEIHPYINATVKYKNKKIGFIYKLNPAFEQANKIYPTFICEVNLNLLLEIADKVHVTSEISKFQKSTRDISFELSNDVNF
DTIVKAMLKDLNYLTNYKVIDKYSDEQMDKENISSLTVKLSFNSLDHQLTEKEINDDFEKILNNLKELKVKVR

Sequences:

>Translated_793_residues
MIITRK*LEKYLDLSNITNEQISVCLNSLGFEVEQEIDYSKLNTKLVIGHIEESDPIEGTKLKKTKTRIGENKYATILST
SRNIEEDKFAIVALPGAKLANGLELENREILGILSEGMFCGFNEIGLPNSVLTEDEQQDVYYIDSIYKNMDEMVGREISE
VLNFDDYTFEVDLTLNRSDALAAKQLVKEIANYFDLKINNNEHKVKFEKPTESITIDLNKKVEDEVNTVSHTFIGLKNTN
TPFDSSHDV*LKHSNVKSAQNKFENIANMATLISGQPFILIDADKVNGELTLTKQTIDEKELIVLKSGNSIVNILGLKTE
DKFKVTEETVTMLVVMLNLDQISMRKQQRNLNVSTVDTQRYAKPLNPNLYDQGIDELVSILDEYKMLEDVQKVVTTVQKQ
NYDNVYSITLEKVQDILGIEITVEEIISLFRTLDIEISVKKSELTFTVDKNRTDLYGKNDLCEEIARLYGYDNIIEQPLE
YVSFKKTKNLDKKLKDKLSDYLVGAGFNNIKTYSLTDIESNKT*NLFKVKDPVVLMSPLSIQRETFRNNLSKSMIDTIIF
NANNGNKSVKFFEMADIFALNGFRQSNLCFGVSGEAITDSLSQVHIKSNYAYISSILMSVLDLYKVDVTNVTFEVNDKAI
DEIHPYINATVKYKNKKIGFIYKLNPAFEQANKIYPTFICEVNLNLLLEIADKVHVTSEISKFQKSTRDISFELSNDVNF
DTIVKAMLKDLNYLTNYKVIDKYSDEQMDKENISSLTVKLSFNSLDHQLTEKEINDDFEKILNNLKELKVKVR
>Mature_793_residues
MIITRK*LEKYLDLSNITNEQISVCLNSLGFEVEQEIDYSKLNTKLVIGHIEESDPIEGTKLKKTKTRIGENKYATILST
SRNIEEDKFAIVALPGAKLANGLELENREILGILSEGMFCGFNEIGLPNSVLTEDEQQDVYYIDSIYKNMDEMVGREISE
VLNFDDYTFEVDLTLNRSDALAAKQLVKEIANYFDLKINNNEHKVKFEKPTESITIDLNKKVEDEVNTVSHTFIGLKNTN
TPFDSSHDV*LKHSNVKSAQNKFENIANMATLISGQPFILIDADKVNGELTLTKQTIDEKELIVLKSGNSIVNILGLKTE
DKFKVTEETVTMLVVMLNLDQISMRKQQRNLNVSTVDTQRYAKPLNPNLYDQGIDELVSILDEYKMLEDVQKVVTTVQKQ
NYDNVYSITLEKVQDILGIEITVEEIISLFRTLDIEISVKKSELTFTVDKNRTDLYGKNDLCEEIARLYGYDNIIEQPLE
YVSFKKTKNLDKKLKDKLSDYLVGAGFNNIKTYSLTDIESNKT*NLFKVKDPVVLMSPLSIQRETFRNNLSKSMIDTIIF
NANNGNKSVKFFEMADIFALNGFRQSNLCFGVSGEAITDSLSQVHIKSNYAYISSILMSVLDLYKVDVTNVTFEVNDKAI
DEIHPYINATVKYKNKKIGFIYKLNPAFEQANKIYPTFICEVNLNLLLEIADKVHVTSEISKFQKSTRDISFELSNDVNF
DTIVKAMLKDLNYLTNYKVIDKYSDEQMDKENISSLTVKLSFNSLDHQLTEKEINDDFEKILNNLKELKVKVR

Specific function: Unknown

COG id: COG0072

COG function: function code J; Phenylalanyl-tRNA synthetase beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788006, Length=758, Percent_Identity=24.2744063324538, Blast_Score=174, Evalue=2e-44,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005146
- InterPro:   IPR005147
- InterPro:   IPR009061
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004532
- InterPro:   IPR020825
- InterPro:   IPR005121
- InterPro:   IPR002547 [H]

Pfam domain/function: PF03483 B3_4; PF03484 B5; PF03147 FDX-ACB; PF01588 tRNA_bind [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 90143; Mature: 90143

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS50886 TRBD ; PS00214 FABP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIITRKLEKYLDLSNITNEQISVCLNSLGFEVEQEIDYSKLNTKLVIGHIEESDPIEGTK
CCCHHHHHHHHCHHCCCHHHHHHHHHHHCCCCHHCCCHHHCCCEEEEEECCCCCCCCCCC
LKKTKTRIGENKYATILSTSRNIEEDKFAIVALPGAKLANGLELENREILGILSEGMFCG
CHHHHHHCCCCCEEEEEECCCCCCCCCEEEEEECCHHHCCCCCCCCCCEEEEHHCCCEEC
FNEIGLPNSVLTEDEQQDVYYIDSIYKNMDEMVGREISEVLNFDDYTFEVDLTLNRSDAL
CCCCCCCCHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCHHH
AAKQLVKEIANYFDLKINNNEHKVKFEKPTESITIDLNKKVEDEVNTVSHTFIGLKNTNT
HHHHHHHHHHHHEEEEECCCCCEEEEECCCCEEEEEECCCHHHHHHHHHHEEEEECCCCC
PFDSSHDVLKHSNVKSAQNKFENIANMATLISGQPFILIDADKVNGELTLTKQTIDEKEL
CCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEECCCCCCEE
IVLKSGNSIVNILGLKTEDKFKVTEETVTMLVVMLNLDQISMRKQQRNLNVSTVDTQRYA
EEEECCCCEEEEEECCCCCCCEEHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEECHHHHC
KPLNPNLYDQGIDELVSILDEYKMLEDVQKVVTTVQKQNYDNVYSITLEKVQDILGIEIT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHCCEEE
VEEIISLFRTLDIEISVKKSELTFTVDKNRTDLYGKNDLCEEIARLYGYDNIIEQPLEYV
HHHHHHHHHHCCEEEEEEECEEEEEEECCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHH
SFKKTKNLDKKLKDKLSDYLVGAGFNNIKTYSLTDIESNKTNLFKVKDPVVLMSPLSIQR
HHHHHCCHHHHHHHHHHHHEECCCCCCCEEEEEEECCCCCCEEEEECCCEEEECCCHHHH
ETFRNNLSKSMIDTIIFNANNGNKSVKFFEMADIFALNGFRQSNLCFGVSGEAITDSLSQ
HHHHHHHHHHHHHHEEEECCCCCCCEEEEEHHHHHHCCCCCCCCEEEECCCHHHHHHHHH
VHIKSNYAYISSILMSVLDLYKVDVTNVTFEVNDKAIDEIHPYINATVKYKNKKIGFIYK
EEEECCHHHHHHHHHHHHHHHEEEEEEEEEEECCHHHHHHHHHHEEEEEECCCEEEEEEE
LNPAFEQANKIYPTFICEVNLNLLLEIADKVHVTSEISKFQKSTRDISFELSNDVNFDTI
ECHHHHHCCCCCEEEEEEECHHEEEEHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHH
VKAMLKDLNYLTNYKVIDKYSDEQMDKENISSLTVKLSFNSLDHQLTEKEINDDFEKILN
HHHHHHHHHHHCCCEEEECCCCHHCCCCCCCEEEEEEEECCHHHHHHHHHCCHHHHHHHH
NLKELKVKVR
HHHHCEEECC
>Mature Secondary Structure
MIITRKLEKYLDLSNITNEQISVCLNSLGFEVEQEIDYSKLNTKLVIGHIEESDPIEGTK
CCCHHHHHHHHCHHCCCHHHHHHHHHHHCCCCHHCCCHHHCCCEEEEEECCCCCCCCCCC
LKKTKTRIGENKYATILSTSRNIEEDKFAIVALPGAKLANGLELENREILGILSEGMFCG
CHHHHHHCCCCCEEEEEECCCCCCCCCEEEEEECCHHHCCCCCCCCCCEEEEHHCCCEEC
FNEIGLPNSVLTEDEQQDVYYIDSIYKNMDEMVGREISEVLNFDDYTFEVDLTLNRSDAL
CCCCCCCCHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCHHH
AAKQLVKEIANYFDLKINNNEHKVKFEKPTESITIDLNKKVEDEVNTVSHTFIGLKNTNT
HHHHHHHHHHHHEEEEECCCCCEEEEECCCCEEEEEECCCHHHHHHHHHHEEEEECCCCC
PFDSSHDVLKHSNVKSAQNKFENIANMATLISGQPFILIDADKVNGELTLTKQTIDEKEL
CCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEECCCCCCEE
IVLKSGNSIVNILGLKTEDKFKVTEETVTMLVVMLNLDQISMRKQQRNLNVSTVDTQRYA
EEEECCCCEEEEEECCCCCCCEEHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEECHHHHC
KPLNPNLYDQGIDELVSILDEYKMLEDVQKVVTTVQKQNYDNVYSITLEKVQDILGIEIT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHCCEEE
VEEIISLFRTLDIEISVKKSELTFTVDKNRTDLYGKNDLCEEIARLYGYDNIIEQPLEYV
HHHHHHHHHHCCEEEEEEECEEEEEEECCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHH
SFKKTKNLDKKLKDKLSDYLVGAGFNNIKTYSLTDIESNKTNLFKVKDPVVLMSPLSIQR
HHHHHCCHHHHHHHHHHHHEECCCCCCCEEEEEEECCCCCCEEEEECCCEEEECCCHHHH
ETFRNNLSKSMIDTIIFNANNGNKSVKFFEMADIFALNGFRQSNLCFGVSGEAITDSLSQ
HHHHHHHHHHHHHHEEEECCCCCCCEEEEEHHHHHHCCCCCCCCEEEECCCHHHHHHHHH
VHIKSNYAYISSILMSVLDLYKVDVTNVTFEVNDKAIDEIHPYINATVKYKNKKIGFIYK
EEEECCHHHHHHHHHHHHHHHEEEEEEEEEEECCHHHHHHHHHHEEEEEECCCEEEEEEE
LNPAFEQANKIYPTFICEVNLNLLLEIADKVHVTSEISKFQKSTRDISFELSNDVNFDTI
ECHHHHHCCCCCEEEEEEECHHEEEEHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHH
VKAMLKDLNYLTNYKVIDKYSDEQMDKENISSLTVKLSFNSLDHQLTEKEINDDFEKILN
HHHHHHHHHHHCCCEEEECCCCHHCCCCCCCEEEEEEEECCHHHHHHHHHCCHHHHHHHH
NLKELKVKVR
HHHHCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA