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The map label for this gene is pncA [H]

Identifier: 50365156

GI number: 50365156

Start: 396060

End: 396551

Strand: Direct

Name: pncA [H]

Synonym: Mfl340

Alternate gene names: 50365156

Gene position: 396060-396551 (Clockwise)

Preceding gene: 50365153

Following gene: 50365157

Centisome position: 49.93

GC content: 27.24

Gene sequence:

>492_bases
ATGAATAAAGCTTTAATAATAGTTGATTATCAGTATGATTTTGTTGATTCAAATGGAAAATTGTATGTGCATGCAGCTGA
AAGTAAAAAAACTTATATAGAATATTTAATTAAAATTTTCAATGAAAACAATGATTTAGTTGTTGCTACAAAGGATATGC
ACCCACTTGATCATTATTCTTTTGCTCAATGAGGACCACATTGTGTTGTTGGAACTAAGGGTACTGATTTATACTTTGAT
TCTTCTTCAATTGATAAGATAATTGAAAAAGGCAAAAATAAAAAAACTGAAAGTTATAGTGCCTTTTTTGATGAAAAAGG
GAATTCTAACTTTCTTGACGAATATTTAAGAGAAAATAATATTCAAGAATTAACTATTGTGGGAGTTGCTTTAGAAGTTT
GTGTTAAAGCAACTTTTGAGCATGCAATTGAGTTGGGTTATAAAACTAATTTAGATTTAAAAGGTTGTGCAGGATTTCAA
GATAAAAAATAA

Upstream 100 bases:

>100_bases
TTTTTAAATCATGATTTGAAACTTTAGTAATGTATAATTTTAGTAAATAACCCTAATTTTTACCACTATTATAATTTATA
TTAAAAACAAAGGAAAATTT

Downstream 100 bases:

>100_bases
AAATACTATTTTATTTAAAACTAATTGATATAATAAAGTTAGTATTTGAACAAATCGAATCTTAAAATTTTAACTTTTAG
TTCATAATTTTATCCATTTG

Product: nicotinamidase/pyrazinamidase

Products: NA

Alternate protein names: PZAase; Nicotine deamidase; NAMase [H]

Number of amino acids: Translated: 163; Mature: 163

Protein sequence:

>163_residues
MNKALIIVDYQYDFVDSNGKLYVHAAESKKTYIEYLIKIFNENNDLVVATKDMHPLDHYSFAQWGPHCVVGTKGTDLYFD
SSSIDKIIEKGKNKKTESYSAFFDEKGNSNFLDEYLRENNIQELTIVGVALEVCVKATFEHAIELGYKTNLDLKGCAGFQ
DKK

Sequences:

>Translated_163_residues
MNKALIIVDYQYDFVDSNGKLYVHAAESKKTYIEYLIKIFNENNDLVVATKDMHPLDHYSFAQ*GPHCVVGTKGTDLYFD
SSSIDKIIEKGKNKKTESYSAFFDEKGNSNFLDEYLRENNIQELTIVGVALEVCVKATFEHAIELGYKTNLDLKGCAGFQ
DKK
>Mature_163_residues
MNKALIIVDYQYDFVDSNGKLYVHAAESKKTYIEYLIKIFNENNDLVVATKDMHPLDHYSFAQ*GPHCVVGTKGTDLYFD
SSSIDKIIEKGKNKKTESYSAFFDEKGNSNFLDEYLRENNIQELTIVGVALEVCVKATFEHAIELGYKTNLDLKGCAGFQ
DKK

Specific function: NAD biosynthesis; nicotinamide to NaMN; first step. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pncA family [H]

Homologues:

Organism=Escherichia coli, GI87081970, Length=178, Percent_Identity=34.2696629213483, Blast_Score=99, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6321401, Length=193, Percent_Identity=33.6787564766839, Blast_Score=77, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: =3.5.1.19 [H]

Molecular weight: Translated: 18365; Mature: 18365

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKALIIVDYQYDFVDSNGKLYVHAAESKKTYIEYLIKIFNENNDLVVATKDMHPLDHYS
CCCEEEEEEEEEEEECCCCEEEEEECCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCC
FAQGPHCVVGTKGTDLYFDSSSIDKIIEKGKNKKTESYSAFFDEKGNSNFLDEYLRENNI
CCCCCEEEEECCCCEEEECCHHHHHHHHCCCCCCCCCHHHHHCCCCCCHHHHHHHHHCCC
QELTIVGVALEVCVKATFEHAIELGYKTNLDLKGCAGFQDKK
CEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNKALIIVDYQYDFVDSNGKLYVHAAESKKTYIEYLIKIFNENNDLVVATKDMHPLDHYS
CCCEEEEEEEEEEEECCCCEEEEEECCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCC
FAQGPHCVVGTKGTDLYFDSSSIDKIIEKGKNKKTESYSAFFDEKGNSNFLDEYLRENNI
CCCCCEEEEECCCCEEEECCHHHHHHHHCCCCCCCCCHHHHHCCCCCCHHHHHHHHHCCC
QELTIVGVALEVCVKATFEHAIELGYKTNLDLKGCAGFQDKK
CEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2670682; 8726014; 9097039; 9278503 [H]