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The map label for this gene is dinB [H]

Identifier: 50365144

GI number: 50365144

Start: 386130

End: 387242

Strand: Reverse

Name: dinB [H]

Synonym: Mfl328

Alternate gene names: 50365144

Gene position: 387242-386130 (Counterclockwise)

Preceding gene: 50365145

Following gene: 50365142

Centisome position: 48.82

GC content: 25.07

Gene sequence:

>1113_bases
ATGAATCAAAAAGTAATTTTGCATCTTGATATGGATGCTTTTTTTGCAAGTTGTGAGCAAGCTCATGATAAGACTTTAAA
AAATAAACCAGTTGTTGTAGCAAGAAATAATGATAGAAGTATTATTGTTGCAGCTTCATATGATGCTAGAAAATATGGGA
TAAAAGCAGGAACGCCTATATTTGAAGCAAAACAACTAGCTTATGGTGAATTAATAATTAAAGAAGGTAATAGAGATCTA
TATGAAGAATATAGCGAAAGAATATTTAATTTAATAAAAGAACAGTTTACATATAAGGTAGAGGTTTTAGGTATCGATGA
ATGTTTTATTGACGCTACTAATATTTGAGAGAAATATGGAAATGTTCAAAATTTATGTTTAGCTATTCAAGCTGCAGTTT
ATCAAGAAACAGGTTTAACTTGTAGTATAGGTGCTAGTTTTACAAGGTTATTTTCAAAAATGGGTAGTGATATGAAAAAA
CCTAATGGAATAACAATAATAACAAAAGAAAATTTTAAAACTTTAATATGAAATAAATCAATCAAAGATGTTATTGGTGT
TGGTAGTTCATCTTTGGAAAAAATGGAGTTATTAAACGTTAAAACAGTAAATGATTTTGTGAAACTTGATGAAAAAATAA
TAGCTGATAGTTTTGGTTTATCTGGACAAAAAATGTATCAAAAATTACATGGAATTAAAAATGATGAAATAGCATATTGA
GAAACATCAGTTAAATCAATATCTAAAGAAAAAACTTTTGATAAGAAAAATATGGCAATTGAAGATATTGAAGAAATATT
GTTTAATTTAGCAGAAAAAGTTGTATTGAGAATGCAAAAGAACAATATTCTTGCAAAAACTGTAACATTGTCAATTAAGT
TTTATAATCAAGAAATTAATTTTGACAAAAAAGAACAAAAAAAAAGAAGAACATATAGTGAAACTTTTAACGAATATACA
GATAGTTTAGAAAAATTATACTCAAGAGTTAAAGTTATTTTAGATGATATTTATGATGGTGAATCTATTTCTTTAATTGG
AATTGGAGTAACTAATTTAATCGAAAAGGATAAACTGGTAAAACAACAAAGTTTTGAAAATATAATTATTTAA

Upstream 100 bases:

>100_bases
TTTAACAAAAACAGAAAAACAAAAGAATTGAAAAGATGAAGAACTAAACAAAGTTGTTAATCAATCTTGAAAATACTTAA
CATAAAAAGGAGGCATCCAC

Downstream 100 bases:

>100_bases
AACTATAAATAATTTAATTTTTATTTTATTGGTATTTTTCATAAAAGAAAGATAGTTAATAAAACTATTTAGAAAATAAC
TAAATTTTTATTTACTATGA

Product: DNA polymerase IV (PolIV)

Products: NA

Alternate protein names: Pol IV [H]

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MNQKVILHLDMDAFFASCEQAHDKTLKNKPVVVARNNDRSIIVAASYDARKYGIKAGTPIFEAKQLAYGELIIKEGNRDL
YEEYSERIFNLIKEQFTYKVEVLGIDECFIDATNIWEKYGNVQNLCLAIQAAVYQETGLTCSIGASFTRLFSKMGSDMKK
PNGITIITKENFKTLIWNKSIKDVIGVGSSSLEKMELLNVKTVNDFVKLDEKIIADSFGLSGQKMYQKLHGIKNDEIAYW
ETSVKSISKEKTFDKKNMAIEDIEEILFNLAEKVVLRMQKNNILAKTVTLSIKFYNQEINFDKKEQKKRRTYSETFNEYT
DSLEKLYSRVKVILDDIYDGESISLIGIGVTNLIEKDKLVKQQSFENIII

Sequences:

>Translated_370_residues
MNQKVILHLDMDAFFASCEQAHDKTLKNKPVVVARNNDRSIIVAASYDARKYGIKAGTPIFEAKQLAYGELIIKEGNRDL
YEEYSERIFNLIKEQFTYKVEVLGIDECFIDATNI*EKYGNVQNLCLAIQAAVYQETGLTCSIGASFTRLFSKMGSDMKK
PNGITIITKENFKTLI*NKSIKDVIGVGSSSLEKMELLNVKTVNDFVKLDEKIIADSFGLSGQKMYQKLHGIKNDEIAY*
ETSVKSISKEKTFDKKNMAIEDIEEILFNLAEKVVLRMQKNNILAKTVTLSIKFYNQEINFDKKEQKKRRTYSETFNEYT
DSLEKLYSRVKVILDDIYDGESISLIGIGVTNLIEKDKLVKQQSFENIII
>Mature_370_residues
MNQKVILHLDMDAFFASCEQAHDKTLKNKPVVVARNNDRSIIVAASYDARKYGIKAGTPIFEAKQLAYGELIIKEGNRDL
YEEYSERIFNLIKEQFTYKVEVLGIDECFIDATNI*EKYGNVQNLCLAIQAAVYQETGLTCSIGASFTRLFSKMGSDMKK
PNGITIITKENFKTLI*NKSIKDVIGVGSSSLEKMELLNVKTVNDFVKLDEKIIADSFGLSGQKMYQKLHGIKNDEIAY*
ETSVKSISKEKTFDKKNMAIEDIEEILFNLAEKVVLRMQKNNILAKTVTLSIKFYNQEINFDKKEQKKRRTYSETFNEYT
DSLEKLYSRVKVILDDIYDGESISLIGIGVTNLIEKDKLVKQQSFENIII

Specific function: Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by polIV. Exhibits

COG id: COG0389

COG function: function code L; Nucleotidyltransferase/DNA polymerase involved in DNA repair

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 umuC domain [H]

Homologues:

Organism=Homo sapiens, GI154350220, Length=342, Percent_Identity=27.4853801169591, Blast_Score=115, Evalue=7e-26,
Organism=Homo sapiens, GI84043967, Length=344, Percent_Identity=23.546511627907, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI7706681, Length=345, Percent_Identity=23.4782608695652, Blast_Score=90, Evalue=3e-18,
Organism=Escherichia coli, GI1786425, Length=348, Percent_Identity=26.4367816091954, Blast_Score=138, Evalue=6e-34,
Organism=Escherichia coli, GI1787432, Length=228, Percent_Identity=28.9473684210526, Blast_Score=102, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI193205700, Length=410, Percent_Identity=26.0975609756098, Blast_Score=94, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI193205702, Length=234, Percent_Identity=29.4871794871795, Blast_Score=65, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6324921, Length=243, Percent_Identity=30.4526748971193, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI19923006, Length=343, Percent_Identity=23.6151603498542, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI21355641, Length=396, Percent_Identity=22.4747474747475, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24644984, Length=396, Percent_Identity=22.4747474747475, Blast_Score=80, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017962
- InterPro:   IPR017961
- InterPro:   IPR001126
- InterPro:   IPR017963
- InterPro:   IPR022880 [H]

Pfam domain/function: PF00817 IMS [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 41781; Mature: 41781

Theoretical pI: Translated: 7.37; Mature: 7.37

Prosite motif: PS50173 UMUC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQKVILHLDMDAFFASCEQAHDKTLKNKPVVVARNNDRSIIVAASYDARKYGIKAGTPI
CCCCEEEEECHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEEECCCCHHCCCCCCCCH
FEAKQLAYGELIIKEGNRDLYEEYSERIFNLIKEQFTYKVEVLGIDECFIDATNIEKYGN
HHHHHHHHCEEEEECCCHHHHHHHHHHHHHHHHHHHEEEEEEEECHHHHEECCCCHHHCC
VQNLCLAIQAAVYQETGLTCSIGASFTRLFSKMGSDMKKPNGITIITKENFKTLINKSIK
HHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHH
DVIGVGSSSLEKMELLNVKTVNDFVKLDEKIIADSFGLSGQKMYQKLHGIKNDEIAYETS
HHHCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCHHHHH
VKSISKEKTFDKKNMAIEDIEEILFNLAEKVVLRMQKNNILAKTVTLSIKFYNQEINFDK
HHHHHHHHCCCHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEEECCCCCCCH
KEQKKRRTYSETFNEYTDSLEKLYSRVKVILDDIYDGESISLIGIGVTNLIEKDKLVKQQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHH
SFENIII
HHCCCCC
>Mature Secondary Structure
MNQKVILHLDMDAFFASCEQAHDKTLKNKPVVVARNNDRSIIVAASYDARKYGIKAGTPI
CCCCEEEEECHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEEECCCCHHCCCCCCCCH
FEAKQLAYGELIIKEGNRDLYEEYSERIFNLIKEQFTYKVEVLGIDECFIDATNIEKYGN
HHHHHHHHCEEEEECCCHHHHHHHHHHHHHHHHHHHEEEEEEEECHHHHEECCCCHHHCC
VQNLCLAIQAAVYQETGLTCSIGASFTRLFSKMGSDMKKPNGITIITKENFKTLINKSIK
HHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHH
DVIGVGSSSLEKMELLNVKTVNDFVKLDEKIIADSFGLSGQKMYQKLHGIKNDEIAYETS
HHHCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCHHHHH
VKSISKEKTFDKKNMAIEDIEEILFNLAEKVVLRMQKNNILAKTVTLSIKFYNQEINFDK
HHHHHHHHCCCHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEEECCCCCCCH
KEQKKRRTYSETFNEYTDSLEKLYSRVKVILDDIYDGESISLIGIGVTNLIEKDKLVKQQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHH
SFENIII
HHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]