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The map label for this gene is ribF [H]

Identifier: 50365099

GI number: 50365099

Start: 325711

End: 326280

Strand: Direct

Name: ribF [H]

Synonym: Mfl283

Alternate gene names: 50365099

Gene position: 325711-326280 (Clockwise)

Preceding gene: 50365098

Following gene: 50365100

Centisome position: 41.06

GC content: 21.4

Gene sequence:

>570_bases
ATGCAAGCGTGGAGGTTTAAATATTTAATGAAAGTTTTTAAAACTGATATAAATAGTATTATCCAAAATGAAAGAGAAAC
TTGTGCAACTATAGGTATGTTTGATGCATTACATAAATATCACAAATTGATTATAGAAAAAACACAAACAATTGCTCAAG
AAAATAATTTGGAATCTATAATAATAACTTTTGATCACAAACCAACAAAAGGAACTGAAACATTATTAAATGAAAATAAA
AAAATTAATTATATTAATGAAAATTTTCAAATAGATAAAATGATAATTTTACAAGTAAATAATGATTTGATTAATACTTC
AAAAGAACAATTTGTCAAAATACTTAAAAATAAATTTAAAGTAATTAAGTTAGTTGAAGGAAGCGACTTTAGATTTGGAT
TTGAGAAAAGTGGAGATGTTGAATACTTAAAAAATAGTTTTGGTGAAAAAAATGTTTTTATTTATGAAAGAGATAAAAAC
ATATCAACATCTAAAATAAAAAAGCTTGTAAAAGAAAATAAAATTGCAGAAATAGATGATGAATTAGAAGTTGACATAAA
TTTATTGTAA

Upstream 100 bases:

>100_bases
AAAAAATAGAAGTGATAGCTGAGAAAAATATTGTTTTTATTTCAAATAAATCAAAAGAACTTATAGCTTGTTATGAGAGA
GAAAATGGAAATGTCTTTAA

Downstream 100 bases:

>100_bases
TAAAGATTTAAAAAGTCTATAATATAAAAAGTGATTGTTCACCAAAGATGTTATTCGCTCCCATGAATGCACCTTTGATG
AAACGCTAAAAATCAAAAAA

Product: riboflavin kinase/FAD synthase

Products: NA

Alternate protein names: Riboflavin kinase; Flavokinase; FMN adenylyltransferase; FAD pyrophosphorylase; FAD synthase [H]

Number of amino acids: Translated: 189; Mature: 189

Protein sequence:

>189_residues
MQAWRFKYLMKVFKTDINSIIQNERETCATIGMFDALHKYHKLIIEKTQTIAQENNLESIIITFDHKPTKGTETLLNENK
KINYINENFQIDKMIILQVNNDLINTSKEQFVKILKNKFKVIKLVEGSDFRFGFEKSGDVEYLKNSFGEKNVFIYERDKN
ISTSKIKKLVKENKIAEIDDELEVDINLL

Sequences:

>Translated_189_residues
MQAWRFKYLMKVFKTDINSIIQNERETCATIGMFDALHKYHKLIIEKTQTIAQENNLESIIITFDHKPTKGTETLLNENK
KINYINENFQIDKMIILQVNNDLINTSKEQFVKILKNKFKVIKLVEGSDFRFGFEKSGDVEYLKNSFGEKNVFIYERDKN
ISTSKIKKLVKENKIAEIDDELEVDINLL
>Mature_189_residues
MQAWRFKYLMKVFKTDINSIIQNERETCATIGMFDALHKYHKLIIEKTQTIAQENNLESIIITFDHKPTKGTETLLNENK
KINYINENFQIDKMIILQVNNDLINTSKEQFVKILKNKFKVIKLVEGSDFRFGFEKSGDVEYLKNSFGEKNVFIYERDKN
ISTSKIKKLVKENKIAEIDDELEVDINLL

Specific function: Unknown

COG id: COG0196

COG function: function code H; FAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribF family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015864
- InterPro:   IPR015865
- InterPro:   IPR002606
- InterPro:   IPR014729 [H]

Pfam domain/function: PF06574 FAD_syn; PF01687 Flavokinase [H]

EC number: =2.7.1.26; =2.7.7.2 [H]

Molecular weight: Translated: 22233; Mature: 22233

Theoretical pI: Translated: 8.40; Mature: 8.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQAWRFKYLMKVFKTDINSIIQNERETCATIGMFDALHKYHKLIIEKTQTIAQENNLESI
CCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
IITFDHKPTKGTETLLNENKKINYINENFQIDKMIILQVNNDLINTSKEQFVKILKNKFK
EEEECCCCCCCHHHHHCCCCEEEEECCCEEEEEEEEEEECCCHHCCCHHHHHHHHHHHCE
VIKLVEGSDFRFGFEKSGDVEYLKNSFGEKNVFIYERDKNISTSKIKKLVKENKIAEIDD
EEEEECCCCEEECCCCCCCHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCC
ELEVDINLL
CEEEEEEEC
>Mature Secondary Structure
MQAWRFKYLMKVFKTDINSIIQNERETCATIGMFDALHKYHKLIIEKTQTIAQENNLESI
CCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
IITFDHKPTKGTETLLNENKKINYINENFQIDKMIILQVNNDLINTSKEQFVKILKNKFK
EEEECCCCCCCHHHHHCCCCEEEEECCCEEEEEEEEEEECCCHHCCCHHHHHHHHHHHCE
VIKLVEGSDFRFGFEKSGDVEYLKNSFGEKNVFIYERDKNISTSKIKKLVKENKIAEIDD
EEEEECCCCEEECCCCCCCHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCC
ELEVDINLL
CEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438 [H]