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The map label for this gene is truB

Identifier: 50365098

GI number: 50365098

Start: 324878

End: 325738

Strand: Direct

Name: truB

Synonym: Mfl282

Alternate gene names: 50365098

Gene position: 324878-325738 (Clockwise)

Preceding gene: 50365097

Following gene: 50365099

Centisome position: 40.96

GC content: 25.2

Gene sequence:

>861_bases
ATGAATAAATCAGGAATAATAATTTTAAATAAACCAAAAGGTTTAACAACAAATCATTTAATTCAAAAACTTAAAAGAAA
ATTAAATGTAAAAAAAATAGGGCATGCAGGTACTTTGGATCCTTTAGCTACTGGTGTGGTTATATGTTTAATAAATAATG
GAACAAAATTAAGTGATTATTTTTTAAATGAAAATAAAGCATATGAAGTCACTATGAAATTATTTCAAGCTACAGATACA
TATGATAGTGATGGAAGCATAATAGAAGAGCAGGAACCTTTCAAAATAGAACAAGAACAAGTTGAAAAAGTTATAGCAAA
GTTTAATGGACTTGCTTATGAACAAGAACCGCCAATGTATTCAGCAATTAAAATTGATGGAAAAAAACTTTATGAATATG
CTCGTGAAAATCAAGTTGTAAAAGTGAATAAAAGACTAATAAAAATTAATTCACTATCTTTAGATAAATATGAAAATAAT
GAAATATCAATGACTGTTTATTGTTCAAAAGGAACTTATATAAGAAGCCTTATTGTAGATATAGCAAAAGAACTAAATAC
AATTGCTCATGTAACTAGTCTAAATAGAATAGAATCAGGAAATTTTATAATTAAAAATTCTGTTAACATTGAAAATTGTG
AAGAATCAAATTTGATTAAAATGTTTGATGCAATTAAAATGGCTGACTATGAAATAGTTGAATTAGATGACACATTAAAT
GTTGAGCATGGTAAAAAAATAGAAGTGATAGCTGAGAAAAATATTGTTTTTATTTCAAATAAATCAAAAGAACTTATAGC
TTGTTATGAGAGAGAAAATGGAAATGTCTTTAAATGCAAGCGTGGAGGTTTAAATATTTAA

Upstream 100 bases:

>100_bases
AAAAAGCATAACTTATAAGCAACTACATAAAAAAATATTTAAATAAGATCACGATCTTATTTTTTATTTTTTTGCATTTA
ATTAAGATAAAATTAATAGT

Downstream 100 bases:

>100_bases
TGAAAGTTTTTAAAACTGATATAAATAGTATTATCCAAAATGAAAGAGAAACTTGTGCAACTATAGGTATGTTTGATGCA
TTACATAAATATCACAAATT

Product: tRNA pseudouridine 5S synthase

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MNKSGIIILNKPKGLTTNHLIQKLKRKLNVKKIGHAGTLDPLATGVVICLINNGTKLSDYFLNENKAYEVTMKLFQATDT
YDSDGSIIEEQEPFKIEQEQVEKVIAKFNGLAYEQEPPMYSAIKIDGKKLYEYARENQVVKVNKRLIKINSLSLDKYENN
EISMTVYCSKGTYIRSLIVDIAKELNTIAHVTSLNRIESGNFIIKNSVNIENCEESNLIKMFDAIKMADYEIVELDDTLN
VEHGKKIEVIAEKNIVFISNKSKELIACYERENGNVFKCKRGGLNI

Sequences:

>Translated_286_residues
MNKSGIIILNKPKGLTTNHLIQKLKRKLNVKKIGHAGTLDPLATGVVICLINNGTKLSDYFLNENKAYEVTMKLFQATDT
YDSDGSIIEEQEPFKIEQEQVEKVIAKFNGLAYEQEPPMYSAIKIDGKKLYEYARENQVVKVNKRLIKINSLSLDKYENN
EISMTVYCSKGTYIRSLIVDIAKELNTIAHVTSLNRIESGNFIIKNSVNIENCEESNLIKMFDAIKMADYEIVELDDTLN
VEHGKKIEVIAEKNIVFISNKSKELIACYERENGNVFKCKRGGLNI
>Mature_286_residues
MNKSGIIILNKPKGLTTNHLIQKLKRKLNVKKIGHAGTLDPLATGVVICLINNGTKLSDYFLNENKAYEVTMKLFQATDT
YDSDGSIIEEQEPFKIEQEQVEKVIAKFNGLAYEQEPPMYSAIKIDGKKLYEYARENQVVKVNKRLIKINSLSLDKYENN
EISMTVYCSKGTYIRSLIVDIAKELNTIAHVTSLNRIESGNFIIKNSVNIENCEESNLIKMFDAIKMADYEIVELDDTLN
VEHGKKIEVIAEKNIVFISNKSKELIACYERENGNVFKCKRGGLNI

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=229, Percent_Identity=37.117903930131, Blast_Score=148, Evalue=5e-36,
Organism=Homo sapiens, GI7661556, Length=214, Percent_Identity=25.7009345794392, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI2367200, Length=235, Percent_Identity=39.5744680851064, Blast_Score=180, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6324037, Length=172, Percent_Identity=31.9767441860465, Blast_Score=84, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6323204, Length=201, Percent_Identity=26.865671641791, Blast_Score=73, Evalue=6e-14,
Organism=Drosophila melanogaster, GI281364189, Length=199, Percent_Identity=28.1407035175879, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI281364187, Length=199, Percent_Identity=28.1407035175879, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI281364185, Length=199, Percent_Identity=28.1407035175879, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI281364183, Length=199, Percent_Identity=28.1407035175879, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI62471759, Length=199, Percent_Identity=28.1407035175879, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI17975520, Length=199, Percent_Identity=28.1407035175879, Blast_Score=72, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_MESFL (Q6F1I4)

Other databases:

- EMBL:   AE017263
- RefSeq:   YP_053523.1
- ProteinModelPortal:   Q6F1I4
- SMR:   Q6F1I4
- GeneID:   2897965
- GenomeReviews:   AE017263_GR
- KEGG:   mfl:Mfl282
- HOGENOM:   HBG397258
- OMA:   YPAHMSK
- ProtClustDB:   CLSK2558354
- BioCyc:   MFLO265311:MFL282-MONOMER
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom

EC number: 4.2.1.70

Molecular weight: Translated: 32452; Mature: 32452

Theoretical pI: Translated: 7.85; Mature: 7.85

Prosite motif: NA

Important sites: ACT_SITE 40-40

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKSGIIILNKPKGLTTNHLIQKLKRKLNVKKIGHAGTLDPLATGVVICLINNGTKLSDY
CCCCCEEEEECCCCCCHHHHHHHHHHHCCHHCCCCCCCCCHHCCCEEEEEECCCCEEEHE
FLNENKAYEVTMKLFQATDTYDSDGSIIEEQEPFKIEQEQVEKVIAKFNGLAYEQEPPMY
EECCCCEEEEEEEEHHHCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCE
SAIKIDGKKLYEYARENQVVKVNKRLIKINSLSLDKYENNEISMTVYCSKGTYIRSLIVD
EEEEECHHHHHHHHHCCCEEEEEEEEEEEECEECCCCCCCEEEEEEEECCCHHHHHHHHH
IAKELNTIAHVTSLNRIESGNFIIKNSVNIENCEESNLIKMFDAIKMADYEIVELDDTLN
HHHHHHHHHHHHHHCEECCCCEEEEECCCCCCCCCCCHHHHHHHHHHCCEEEEEECCCCC
VEHGKKIEVIAEKNIVFISNKSKELIACYERENGNVFKCKRGGLNI
CCCCCEEEEEEECCEEEEECCCCEEEEEEECCCCCEEEEECCCCCC
>Mature Secondary Structure
MNKSGIIILNKPKGLTTNHLIQKLKRKLNVKKIGHAGTLDPLATGVVICLINNGTKLSDY
CCCCCEEEEECCCCCCHHHHHHHHHHHCCHHCCCCCCCCCHHCCCEEEEEECCCCEEEHE
FLNENKAYEVTMKLFQATDTYDSDGSIIEEQEPFKIEQEQVEKVIAKFNGLAYEQEPPMY
EECCCCEEEEEEEEHHHCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCE
SAIKIDGKKLYEYARENQVVKVNKRLIKINSLSLDKYENNEISMTVYCSKGTYIRSLIVD
EEEEECHHHHHHHHHCCCEEEEEEEEEEEECEECCCCCCCEEEEEEEECCCHHHHHHHHH
IAKELNTIAHVTSLNRIESGNFIIKNSVNIENCEESNLIKMFDAIKMADYEIVELDDTLN
HHHHHHHHHHHHHHCEECCCCEEEEECCCCCCCCCCCHHHHHHHHHHCCEEEEEECCCCC
VEHGKKIEVIAEKNIVFISNKSKELIACYERENGNVFKCKRGGLNI
CCCCCEEEEEEECCEEEEECCCCEEEEEEECCCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA