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The map label for this gene is sigA [H]

Identifier: 50365086

GI number: 50365086

Start: 309823

End: 311238

Strand: Direct

Name: sigA [H]

Synonym: Mfl270

Alternate gene names: 50365086

Gene position: 309823-311238 (Clockwise)

Preceding gene: 50365085

Following gene: 50365087

Centisome position: 39.06

GC content: 27.82

Gene sequence:

>1416_bases
ATGAAAAAATATATGGATAAAAAAGAAATAGCAAAAATTAAAACAATTGAAGATTTTTATGAAGCAACAGTAGAATTTGC
AAAACAAAATAATAATGAAATAACATCTGAAGAAGTTCAAATGAGTTTTAGTAAAATATTTGCAAATGCAACTGATAATG
AATATGAAAAATTATTAGAAGATTTACAAGCAAAAGGAATTCAATTCACAGATTTAGAAGATATTGACATTGATGAAGAA
ATAGATCTTGATGAAGAAGTTGATGAAGATGCTGAAATAAGTGATGATGATGCTTATGCTGATGAATTAATTGGAGAAAG
AAAAGGTCCAGGTAGAAGACCAAAAGATGCTGGTACAACTAAATATAGAGTTGGATCAATTTCAAATGAAACTAAAATTC
AAGATTTAATTAAAACATACTTTTCAACTATTGGACAAACTAAAATTTTAACTAAAGATCAAGAAATTGTTTACGCTAAA
TTAGCTAATTCAGAAGATCCAGAAGAAAGAAAAGAAGGAAGAGATATGTTAATCACTTCTAACTTAAAATTAGTTATTTC
AGTTGCTAGAAAACACTTAAATAGAGGTTTAGATTTTGCAGATTTAATTGAAGAAGGAAATATTGGTTTAATTAAAGCTG
TTGATAAATTTGATTATGAAAAAGGGTTTAAGTTTTCAACATATGCAACATGATGAATTCGTCAAGCTATAACAAGAGCT
ATTGCTGATCAAGCTAGAACAATTAGAATACCAGTTCATATGGTTGAAACAATTAATAAACTTTCTAGAATTGAAAGACA
ATTAACTCAAGAACTAGGAAGAGAACCATCATCAAAAGAAGTTGCAGAAAGAATGGGTGGAGACATGACAGCTGAAAAAG
TTGTTGAAATTAAAAAAATTGCTGTTGAACCTGTTAGTTTAGAAAAACCATTTGGTGATGAAGATGACACTCATTTTGGA
GATTTCGTTGAAGATAAAGATATGATTTCTCCAACAGACTTTACTGAAAAAGAAATTTTAAGAGAAGTTATAGACAAAGT
ATTTGAAGATATGCCAGCAAGAGAAGAAAAAGTTATTCGTATGAGATACGGGATTGTTCCAACAAAAGTTAGAACATTGA
TTAGACTTGCTGAAGAATGTAATGATGAAACTGCTGGAGAATTAGCTAAAGCAATTAATAAACTAGATATTCACTTAGAG
ACTCCTGTTGAAAAAATAAGAACAGTTGATAGTAAAATAATTCAAGACCATTTATTAAAATATGAAGCATCAAAAACTTT
AGAAGAAGTTGGTAAAGAATTAAATGTTACAAGAGAAAGAATTAGACAAATTGAAGCTAAAACAATTAGAAAATTAAAAC
AACCTGCAAATGCTAATAAATCAGGTAAAGTTTTAAAAGAATTTTACAAAGGTTAA

Upstream 100 bases:

>100_bases
CGAGATAATTTAATGGACATTTTATCTGAAAGAATTAAAAAAAGAGAAGAATGACTAGGAAATTTTAACGATAAGAACAA
CTAATGAAAGAGGTAACTAT

Downstream 100 bases:

>100_bases
TATGCTGACAAAAAGACTAAGAACTATTGCTGAATTAATTGATTCTTGCAATGTTGTCGCAGATATTGGTACAGATCATG
CATATTTACCAATAACTTTA

Product: RNA polymerase sigma factor

Products: NA

Alternate protein names: Sigma-43; Sigma-A [H]

Number of amino acids: Translated: 471; Mature: 471

Protein sequence:

>471_residues
MKKYMDKKEIAKIKTIEDFYEATVEFAKQNNNEITSEEVQMSFSKIFANATDNEYEKLLEDLQAKGIQFTDLEDIDIDEE
IDLDEEVDEDAEISDDDAYADELIGERKGPGRRPKDAGTTKYRVGSISNETKIQDLIKTYFSTIGQTKILTKDQEIVYAK
LANSEDPEERKEGRDMLITSNLKLVISVARKHLNRGLDFADLIEEGNIGLIKAVDKFDYEKGFKFSTYATWWIRQAITRA
IADQARTIRIPVHMVETINKLSRIERQLTQELGREPSSKEVAERMGGDMTAEKVVEIKKIAVEPVSLEKPFGDEDDTHFG
DFVEDKDMISPTDFTEKEILREVIDKVFEDMPAREEKVIRMRYGIVPTKVRTLIRLAEECNDETAGELAKAINKLDIHLE
TPVEKIRTVDSKIIQDHLLKYEASKTLEEVGKELNVTRERIRQIEAKTIRKLKQPANANKSGKVLKEFYKG

Sequences:

>Translated_471_residues
MKKYMDKKEIAKIKTIEDFYEATVEFAKQNNNEITSEEVQMSFSKIFANATDNEYEKLLEDLQAKGIQFTDLEDIDIDEE
IDLDEEVDEDAEISDDDAYADELIGERKGPGRRPKDAGTTKYRVGSISNETKIQDLIKTYFSTIGQTKILTKDQEIVYAK
LANSEDPEERKEGRDMLITSNLKLVISVARKHLNRGLDFADLIEEGNIGLIKAVDKFDYEKGFKFSTYAT**IRQAITRA
IADQARTIRIPVHMVETINKLSRIERQLTQELGREPSSKEVAERMGGDMTAEKVVEIKKIAVEPVSLEKPFGDEDDTHFG
DFVEDKDMISPTDFTEKEILREVIDKVFEDMPAREEKVIRMRYGIVPTKVRTLIRLAEECNDETAGELAKAINKLDIHLE
TPVEKIRTVDSKIIQDHLLKYEASKTLEEVGKELNVTRERIRQIEAKTIRKLKQPANANKSGKVLKEFYKG
>Mature_471_residues
MKKYMDKKEIAKIKTIEDFYEATVEFAKQNNNEITSEEVQMSFSKIFANATDNEYEKLLEDLQAKGIQFTDLEDIDIDEE
IDLDEEVDEDAEISDDDAYADELIGERKGPGRRPKDAGTTKYRVGSISNETKIQDLIKTYFSTIGQTKILTKDQEIVYAK
LANSEDPEERKEGRDMLITSNLKLVISVARKHLNRGLDFADLIEEGNIGLIKAVDKFDYEKGFKFSTYAT**IRQAITRA
IADQARTIRIPVHMVETINKLSRIERQLTQELGREPSSKEVAERMGGDMTAEKVVEIKKIAVEPVSLEKPFGDEDDTHFG
DFVEDKDMISPTDFTEKEILREVIDKVFEDMPAREEKVIRMRYGIVPTKVRTLIRLAEECNDETAGELAKAINKLDIHLE
TPVEKIRTVDSKIIQDHLLKYEASKTLEEVGKELNVTRERIRQIEAKTIRKLKQPANANKSGKVLKEFYKG

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=292, Percent_Identity=46.9178082191781, Blast_Score=254, Evalue=9e-69,
Organism=Escherichia coli, GI1789098, Length=288, Percent_Identity=34.7222222222222, Blast_Score=172, Evalue=4e-44,
Organism=Escherichia coli, GI1788231, Length=166, Percent_Identity=29.5180722891566, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1789871, Length=247, Percent_Identity=25.9109311740891, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 53637; Mature: 53637

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKYMDKKEIAKIKTIEDFYEATVEFAKQNNNEITSEEVQMSFSKIFANATDNEYEKLLE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHH
DLQAKGIQFTDLEDIDIDEEIDLDEEVDEDAEISDDDAYADELIGERKGPGRRPKDAGTT
HHHHCCCEECCCCCCCCCCCCCCCHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCC
KYRVGSISNETKIQDLIKTYFSTIGQTKILTKDQEIVYAKLANSEDPEERKEGRDMLITS
CEEECCCCCHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHCCCCCHHHHHCCCCEEEEC
NLKLVISVARKHLNRGLDFADLIEEGNIGLIKAVDKFDYEKGFKFSTYATIRQAITRAIA
CHHHHHHHHHHHHHCCCCHHHHHHCCCCEEEEHHHHHHHHCCCCHHHHHHHHHHHHHHHH
DQARTIRIPVHMVETINKLSRIERQLTQELGREPSSKEVAERMGGDMTAEKVVEIKKIAV
HCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHC
EPVSLEKPFGDEDDTHFGDFVEDKDMISPTDFTEKEILREVIDKVFEDMPAREEKVIRMR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHH
YGIVPTKVRTLIRLAEECNDETAGELAKAINKLDIHLETPVEKIRTVDSKIIQDHLLKYE
HCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCEEECCHHHHHHHHHHHHHHHHHHHHH
ASKTLEEVGKELNVTRERIRQIEAKTIRKLKQPANANKSGKVLKEFYKG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MKKYMDKKEIAKIKTIEDFYEATVEFAKQNNNEITSEEVQMSFSKIFANATDNEYEKLLE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHH
DLQAKGIQFTDLEDIDIDEEIDLDEEVDEDAEISDDDAYADELIGERKGPGRRPKDAGTT
HHHHCCCEECCCCCCCCCCCCCCCHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCC
KYRVGSISNETKIQDLIKTYFSTIGQTKILTKDQEIVYAKLANSEDPEERKEGRDMLITS
CEEECCCCCHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHCCCCCHHHHHCCCCEEEEC
NLKLVISVARKHLNRGLDFADLIEEGNIGLIKAVDKFDYEKGFKFSTYATIRQAITRAIA
CHHHHHHHHHHHHHCCCCHHHHHHCCCCEEEEHHHHHHHHCCCCHHHHHHHHHHHHHHHH
DQARTIRIPVHMVETINKLSRIERQLTQELGREPSSKEVAERMGGDMTAEKVVEIKKIAV
HCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHC
EPVSLEKPFGDEDDTHFGDFVEDKDMISPTDFTEKEILREVIDKVFEDMPAREEKVIRMR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHH
YGIVPTKVRTLIRLAEECNDETAGELAKAINKLDIHLETPVEKIRTVDSKIIQDHLLKYE
HCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCEEECCHHHHHHHHHHHHHHHHHHHHH
ASKTLEEVGKELNVTRERIRQIEAKTIRKLKQPANANKSGKVLKEFYKG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]