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The map label for this gene is dnaG [H]

Identifier: 50365085

GI number: 50365085

Start: 307926

End: 309806

Strand: Direct

Name: dnaG [H]

Synonym: Mfl269

Alternate gene names: 50365085

Gene position: 307926-309806 (Clockwise)

Preceding gene: 50365084

Following gene: 50365086

Centisome position: 38.82

GC content: 24.83

Gene sequence:

>1881_bases
GTGATACTCATGTTAATACCAAAAGAAGTTATTGATGATATTATTGAAAAATCAGACATAGTTTCAATTGTAAGCGAAAG
AGTAGCGCTTTCAAAAAAAGGTAGAAACTTTTGAGGGTTATGTCCATTTCATCAAGACCAAAATGCTTCAATGTCTGTTT
CACCAGAAAAAAAATTTTTCAAATGTTTTTCTTGTCAAGTTTCTGGTACAGTTTTAGACTTTGTTAAAGACTTTGATAAT
ATAAGTTTTCAAGAAGCTATAAAAAAATTAGCAGTTATATTAAACTATGATTTGTCTAAGTTTGAAAATGAAAATCCAGT
TAAACAAAATAAAAATACAATTATTTATAAACTTAATGAAGAATCTTTAGCATTTTTTAAATTAAACTTAAGATCTAATG
AAGCAAAGCATGCTGTCAAATACCTTCATTCAAGAGACATAACAAATGAAGATATTGCATTTTTTGAAATAGGATTCTTA
CCAAAGAATAATAGTTTAGTTGAACACTTAATAAGCAAAGGATACAACATATCAGATATTGTTGAAGCTGGATTGGGGAC
TTATAACGAAGAACGCCAAAAAATGTATGATATTTTTACTGATAGAATAATGTTTCCAATAAGAAATGAAAATAAAGAAT
TAATTGGTTTTAGTGGAAGAATTATTGAAACAAATTCTGAAAAACCAAAATATTTAAATACAAAAGAAACACCAGTATTT
TCAAAAAGAAAAATAGCTTACAACTTTAGTGAGGCTATTAAATCAGCGCGAGTTAAAAAAGAATTAATTGTTTTAGAAGG
ATACATGGATGTTATCAGTTTGCATAAAAATGGAATAGATAATACAATCGCTTTAATGGGAACCGCTCTTTCACAATATC
ATGTTAGTATTTTCAAAAATATTAAAGGAACTATAAAAATGTTCTTGGATGGTGATGAGCCTGGTATAAAGGGTAATATA
GAGGCTTCCCAAACATTAATTTTAAATAATCAAAAGGTACTGGTAGTTAATAACCCTACAAATAACGATCCAGATGAATT
AATAAAACAAGGTAAAAAAGCTGAAATTGAAAAAATGATTTCTGAAGCTTTAAATCCTTTGGAATACATTATTTCAAAAC
GTTGACCAAAAGTTAATTCAAAAGATTTTAATTCTGTTGAAGAATTCATGAAAGAAATATGTTCATTTGTATTAAAGTGT
AACAACAATATACTTTATGAAACTTCAATTGATGAACTTGAAAAAATAACAGGTCTTTCAAAAGAAGCAATTATTAATTT
TTATAAAAAAATATTAAATAAACAAATCAATAGTATTTCTAATAATAAGGTTGAAGAAGTTAAAACAAGTTCTGAAAAAC
AAGCAGTTAATAATAAAAATACGAGTTTTAATATTTTGTCTTCTTTGAAAGCTTATGAATTGGCAGAAAAAACTATTTTA
TTTGATTTAATAAAATCTAATAAAAACTTAGAATTGATAAAAGATAAAATTAGAGAAGTAAAATTCCCACATAAAGAATT
TGGAAGATTAATTTTAAAAATAATTAATTCTTATGAAGAGAACATGAATTACAATGAGACTGAAATAAAAGAAATAATTA
ATCAAAACTTTTCTCAAGAAACACTTGAAGAAATAAACTTGTATGAATCTGATCCTTTAATGGTAAGAATAAAACCTGAA
GCAAATACAGCTAAGTTAATTGAAAATTCATTTGAAAAATTAAAAATGTATTCTAATGAGAAAAAAATAGCAGAAATTAA
TGAAATGTTAAATTCAAATAATTTGAGTAAAAACGATCGAGATAATTTAATGGACATTTTATCTGAAAGAATTAAAAAAA
GAGAAGAATGACTAGGAAATTTTAACGATAAGAACAACTAA

Upstream 100 bases:

>100_bases
ACAGTAAGAGAAAGAGACTCAATGGAACAAGTACGTATTAATTTAGATGAACTTGAAGCATATTTAAAAGCAAAATTTTA
ATTTTTAATTTTGAAAGGAA

Downstream 100 bases:

>100_bases
TGAAAGAGGTAACTATATGAAAAAATATATGGATAAAAAAGAAATAGCAAAAATTAAAACAATTGAAGATTTTTATGAAG
CAACAGTAGAATTTGCAAAA

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 626; Mature: 626

Protein sequence:

>626_residues
MILMLIPKEVIDDIIEKSDIVSIVSERVALSKKGRNFWGLCPFHQDQNASMSVSPEKKFFKCFSCQVSGTVLDFVKDFDN
ISFQEAIKKLAVILNYDLSKFENENPVKQNKNTIIYKLNEESLAFFKLNLRSNEAKHAVKYLHSRDITNEDIAFFEIGFL
PKNNSLVEHLISKGYNISDIVEAGLGTYNEERQKMYDIFTDRIMFPIRNENKELIGFSGRIIETNSEKPKYLNTKETPVF
SKRKIAYNFSEAIKSARVKKELIVLEGYMDVISLHKNGIDNTIALMGTALSQYHVSIFKNIKGTIKMFLDGDEPGIKGNI
EASQTLILNNQKVLVVNNPTNNDPDELIKQGKKAEIEKMISEALNPLEYIISKRWPKVNSKDFNSVEEFMKEICSFVLKC
NNNILYETSIDELEKITGLSKEAIINFYKKILNKQINSISNNKVEEVKTSSEKQAVNNKNTSFNILSSLKAYELAEKTIL
FDLIKSNKNLELIKDKIREVKFPHKEFGRLILKIINSYEENMNYNETEIKEIINQNFSQETLEEINLYESDPLMVRIKPE
ANTAKLIENSFEKLKMYSNEKKIAEINEMLNSNNLSKNDRDNLMDILSERIKKREEWLGNFNDKNN

Sequences:

>Translated_626_residues
MILMLIPKEVIDDIIEKSDIVSIVSERVALSKKGRNF*GLCPFHQDQNASMSVSPEKKFFKCFSCQVSGTVLDFVKDFDN
ISFQEAIKKLAVILNYDLSKFENENPVKQNKNTIIYKLNEESLAFFKLNLRSNEAKHAVKYLHSRDITNEDIAFFEIGFL
PKNNSLVEHLISKGYNISDIVEAGLGTYNEERQKMYDIFTDRIMFPIRNENKELIGFSGRIIETNSEKPKYLNTKETPVF
SKRKIAYNFSEAIKSARVKKELIVLEGYMDVISLHKNGIDNTIALMGTALSQYHVSIFKNIKGTIKMFLDGDEPGIKGNI
EASQTLILNNQKVLVVNNPTNNDPDELIKQGKKAEIEKMISEALNPLEYIISKR*PKVNSKDFNSVEEFMKEICSFVLKC
NNNILYETSIDELEKITGLSKEAIINFYKKILNKQINSISNNKVEEVKTSSEKQAVNNKNTSFNILSSLKAYELAEKTIL
FDLIKSNKNLELIKDKIREVKFPHKEFGRLILKIINSYEENMNYNETEIKEIINQNFSQETLEEINLYESDPLMVRIKPE
ANTAKLIENSFEKLKMYSNEKKIAEINEMLNSNNLSKNDRDNLMDILSERIKKREE*LGNFNDKNN
>Mature_626_residues
MILMLIPKEVIDDIIEKSDIVSIVSERVALSKKGRNF*GLCPFHQDQNASMSVSPEKKFFKCFSCQVSGTVLDFVKDFDN
ISFQEAIKKLAVILNYDLSKFENENPVKQNKNTIIYKLNEESLAFFKLNLRSNEAKHAVKYLHSRDITNEDIAFFEIGFL
PKNNSLVEHLISKGYNISDIVEAGLGTYNEERQKMYDIFTDRIMFPIRNENKELIGFSGRIIETNSEKPKYLNTKETPVF
SKRKIAYNFSEAIKSARVKKELIVLEGYMDVISLHKNGIDNTIALMGTALSQYHVSIFKNIKGTIKMFLDGDEPGIKGNI
EASQTLILNNQKVLVVNNPTNNDPDELIKQGKKAEIEKMISEALNPLEYIISKR*PKVNSKDFNSVEEFMKEICSFVLKC
NNNILYETSIDELEKITGLSKEAIINFYKKILNKQINSISNNKVEEVKTSSEKQAVNNKNTSFNILSSLKAYELAEKTIL
FDLIKSNKNLELIKDKIREVKFPHKEFGRLILKIINSYEENMNYNETEIKEIINQNFSQETLEEINLYESDPLMVRIKPE
ANTAKLIENSFEKLKMYSNEKKIAEINEMLNSNNLSKNDRDNLMDILSERIKKREE*LGNFNDKNN

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=381, Percent_Identity=30.7086614173228, Blast_Score=219, Evalue=5e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016136
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR013173
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 71690; Mature: 71690

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILMLIPKEVIDDIIEKSDIVSIVSERVALSKKGRNFGLCPFHQDQNASMSVSPEKKFFK
CEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHH
CFSCQVSGTVLDFVKDFDNISFQEAIKKLAVILNYDLSKFENENPVKQNKNTIIYKLNEE
HHHCEECHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCEEEEEECCC
SLAFFKLNLRSNEAKHAVKYLHSRDITNEDIAFFEIGFLPKNNSLVEHLISKGYNISDIV
CEEEEEEECCCCHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCHHHHHHHHCCCCHHHHH
EAGLGTYNEERQKMYDIFTDRIMFPIRNENKELIGFSGRIIETNSEKPKYLNTKETPVFS
HHCCCCCCHHHHHHHHHHHHHEEEEEECCCCCEEECCCEEEECCCCCCCCCCCCCCCCCC
KRKIAYNFSEAIKSARVKKELIVLEGYMDVISLHKNGIDNTIALMGTALSQYHVSIFKNI
CHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHC
KGTIKMFLDGDEPGIKGNIEASQTLILNNQKVLVVNNPTNNDPDELIKQGKKAEIEKMIS
CCEEEEEECCCCCCCCCCCCCCEEEEECCCEEEEECCCCCCCHHHHHHCCCHHHHHHHHH
EALNPLEYIISKRPKVNSKDFNSVEEFMKEICSFVLKCNNNILYETSIDELEKITGLSKE
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHCCCHH
AIINFYKKILNKQINSISNNKVEEVKTSSEKQAVNNKNTSFNILSSLKAYELAEKTILFD
HHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
LIKSNKNLELIKDKIREVKFPHKEFGRLILKIINSYEENMNYNETEIKEIINQNFSQETL
HHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHH
EEINLYESDPLMVRIKPEANTAKLIENSFEKLKMYSNEKKIAEINEMLNSNNLSKNDRDN
HHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHH
LMDILSERIKKREELGNFNDKNN
HHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MILMLIPKEVIDDIIEKSDIVSIVSERVALSKKGRNFGLCPFHQDQNASMSVSPEKKFFK
CEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHH
CFSCQVSGTVLDFVKDFDNISFQEAIKKLAVILNYDLSKFENENPVKQNKNTIIYKLNEE
HHHCEECHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCEEEEEECCC
SLAFFKLNLRSNEAKHAVKYLHSRDITNEDIAFFEIGFLPKNNSLVEHLISKGYNISDIV
CEEEEEEECCCCHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCHHHHHHHHCCCCHHHHH
EAGLGTYNEERQKMYDIFTDRIMFPIRNENKELIGFSGRIIETNSEKPKYLNTKETPVFS
HHCCCCCCHHHHHHHHHHHHHEEEEEECCCCCEEECCCEEEECCCCCCCCCCCCCCCCCC
KRKIAYNFSEAIKSARVKKELIVLEGYMDVISLHKNGIDNTIALMGTALSQYHVSIFKNI
CHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHC
KGTIKMFLDGDEPGIKGNIEASQTLILNNQKVLVVNNPTNNDPDELIKQGKKAEIEKMIS
CCEEEEEECCCCCCCCCCCCCCEEEEECCCEEEEECCCCCCCHHHHHHCCCHHHHHHHHH
EALNPLEYIISKRPKVNSKDFNSVEEFMKEICSFVLKCNNNILYETSIDELEKITGLSKE
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHCCCHH
AIINFYKKILNKQINSISNNKVEEVKTSSEKQAVNNKNTSFNILSSLKAYELAEKTILFD
HHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
LIKSNKNLELIKDKIREVKFPHKEFGRLILKIINSYEENMNYNETEIKEIINQNFSQETL
HHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHH
EEINLYESDPLMVRIKPEANTAKLIENSFEKLKMYSNEKKIAEINEMLNSNNLSKNDRDN
HHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHH
LMDILSERIKKREELGNFNDKNN
HHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]