Click here to switch to the map view.

The map label for this gene is ygfA [C]

Identifier: 50365066

GI number: 50365066

Start: 279986

End: 280633

Strand: Direct

Name: ygfA [C]

Synonym: Mfl250

Alternate gene names: 50365066

Gene position: 279986-280633 (Clockwise)

Preceding gene: 50365065

Following gene: 50365067

Centisome position: 35.3

GC content: 23.92

Gene sequence:

>648_bases
ATGAGCAACAAGAATCAAATAAGAGAAAAATTTCTTAAAACAAGATCTTTTTTATCAAAATCATACAAAGAAGAAGTTAA
TAAAATTATCGAAAGAAAAGTTAATCATTATATAGATAGATATAACTTAGAAAAATATGCCATTTATCTTTCAACTGAGA
ATGAACCTAATACTTTAAACATTATTGAAACAAGTTTAAAAAAAGGAATAGAAGTTTATGTTCCATTAATTATTGAAGAT
AACAAAATGGAATTCAAGAAAATTACAAATTTAGAAACTGATTTAGAACAAAATAAGGTTTTAAACATTTTACAACCAAA
ATCAACATGTTCAACATTAGAAAATGGTAACTACATTAATACTATGTTTATTCCATTGGTAGCTTTTGATAAACAATTAA
ATAGAGTTGGTATGGGTAAAGGGTTTTATGATCGTTGATTAAATGAAAATGATTACATTGGATATAAAATTGGTTTATCA
GCTTCAACTCAATTATCAAATGAAAGCATTGATGCTGATGAGTTTGATGTTAAATTTGATAATGTAATTACAGAAAAAGA
AATTTATGTTCCATTTGTTGAAGAAGAACAAGAATTTGATTATGATGTTACTTATTCAGTTTTTAACGATGAAACTATTG
TAGGTTAA

Upstream 100 bases:

>100_bases
TGCTAATTTAGCAATTGAAGCCATAAATGAGATTATTGAATAGTTTGTTTATGTATAATTATATTATGTAATAATAAATT
ATATTTTTAAAGGAGAAACA

Downstream 100 bases:

>100_bases
TTGAAATAGGTTTTTACCTATTTTTTTTATTTTCTCTTGCATTAAAGTAGGTTTAATCTTTTAAAATAATAAATATAGAA
CAAAAGAGGTCATTATGGAA

Product: 5-formyltetrahydrofolate cyclo-ligase family protein

Products: ADP; phosphate; 5,10-methenyltetrahydrofolate

Alternate protein names: NA

Number of amino acids: Translated: 215; Mature: 214

Protein sequence:

>215_residues
MSNKNQIREKFLKTRSFLSKSYKEEVNKIIERKVNHYIDRYNLEKYAIYLSTENEPNTLNIIETSLKKGIEVYVPLIIED
NKMEFKKITNLETDLEQNKVLNILQPKSTCSTLENGNYINTMFIPLVAFDKQLNRVGMGKGFYDRWLNENDYIGYKIGLS
ASTQLSNESIDADEFDVKFDNVITEKEIYVPFVEEEQEFDYDVTYSVFNDETIVG

Sequences:

>Translated_215_residues
MSNKNQIREKFLKTRSFLSKSYKEEVNKIIERKVNHYIDRYNLEKYAIYLSTENEPNTLNIIETSLKKGIEVYVPLIIED
NKMEFKKITNLETDLEQNKVLNILQPKSTCSTLENGNYINTMFIPLVAFDKQLNRVGMGKGFYDR*LNENDYIGYKIGLS
ASTQLSNESIDADEFDVKFDNVITEKEIYVPFVEEEQEFDYDVTYSVFNDETIVG
>Mature_214_residues
SNKNQIREKFLKTRSFLSKSYKEEVNKIIERKVNHYIDRYNLEKYAIYLSTENEPNTLNIIETSLKKGIEVYVPLIIEDN
KMEFKKITNLETDLEQNKVLNILQPKSTCSTLENGNYINTMFIPLVAFDKQLNRVGMGKGFYDR*LNENDYIGYKIGLSA
STQLSNESIDADEFDVKFDNVITEKEIYVPFVEEEQEFDYDVTYSVFNDETIVG

Specific function: Unknown

COG id: COG0212

COG function: function code H; 5-formyltetrahydrofolate cyclo-ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5-formyltetrahydrofolate cyclo-ligase family [H]

Homologues:

Organism=Escherichia coli, GI1789278, Length=179, Percent_Identity=25.6983240223464, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002698 [H]

Pfam domain/function: PF01812 5-FTHF_cyc-lig [H]

EC number: 6.3.3.2

Molecular weight: Translated: 25014; Mature: 24882

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNKNQIREKFLKTRSFLSKSYKEEVNKIIERKVNHYIDRYNLEKYAIYLSTENEPNTLN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCEEH
IIETSLKKGIEVYVPLIIEDNKMEFKKITNLETDLEQNKVLNILQPKSTCSTLENGNYIN
EEHHHHHCCCEEEEEEEEECCCHHHHHHHCCHHCHHHHHHEEEECCCHHHHHHCCCCEEE
TMFIPLVAFDKQLNRVGMGKGFYDRLNENDYIGYKIGLSASTQLSNESIDADEFDVKFDN
HHHHHHHHHHHHHHHHCCCCCHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCEECCC
VITEKEIYVPFVEEEQEFDYDVTYSVFNDETIVG
EECCCEEEEEEECCCCCCCCEEEEEEECCCCCCC
>Mature Secondary Structure 
SNKNQIREKFLKTRSFLSKSYKEEVNKIIERKVNHYIDRYNLEKYAIYLSTENEPNTLN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCEEH
IIETSLKKGIEVYVPLIIEDNKMEFKKITNLETDLEQNKVLNILQPKSTCSTLENGNYIN
EEHHHHHCCCEEEEEEEEECCCHHHHHHHCCHHCHHHHHHEEEECCCHHHHHHCCCCEEE
TMFIPLVAFDKQLNRVGMGKGFYDRLNENDYIGYKIGLSASTQLSNESIDADEFDVKFDN
HHHHHHHHHHHHHHHHCCCCCHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCEECCC
VITEKEIYVPFVEEEQEFDYDVTYSVFNDETIVG
EECCCEEEEEEECCCCCCCCEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; 5-formyltetrahydrofolate

Specific reaction: ATP + 5-formyltetrahydrofolate = ADP + phosphate + 5,10-methenyltetrahydrofolate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]