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The map label for this gene is plsX

Identifier: 50365046

GI number: 50365046

Start: 254377

End: 255372

Strand: Direct

Name: plsX

Synonym: Mfl230

Alternate gene names: 50365046

Gene position: 254377-255372 (Clockwise)

Preceding gene: 50365045

Following gene: 50365047

Centisome position: 32.07

GC content: 28.41

Gene sequence:

>996_bases
ATGTATAAAATAGCGTTTGATGTTATGGGTTCAGATAATGGTAGTGCAGTTGCAATAGAAGCTGCAAGCAGATTTATAAA
ATCAAGAAAAGATTTATATCTTGTTTTTGTTGGTGATGAAGACCAAATAAAAACATCTTTAGAAAAATTCCCAATTGACG
AGTCAAGATTTGAAATTTTAGCAACAAAAGAATTTATTGATATGAATGGTTCAATTATGGATATTAGAAGAAAAAAAGAT
TCATCTATGGTTAGAGCTTTAGAAACTTTAAAGGATAAAAAAGTTGATGCAATGATTACTGGTGGAAATTCTGCTGCATT
TATTGCAGGTTCACATTTTATTCTTGGTGAATTAAATGGAATTTCTAGACCAGGGTTCATGCCAACTCTTCCAACAGCTG
TAAGCAATAAATTAACTTTATTGCTTGATGTAGGGGCAAATCTTGAAGCTGACATTGAAGATATCATAGGATATGCAAAA
ATGGCTAATATATATGCTAAAAACGTCTTAAAAATAGAAAATCCTTTGATTGCACAATTAAATATAGGTGAAGAAAAGTC
AAAAGGTACTTTATTACAAAAAGAAATATATAAAGAATTAGAATCAGATGAGAATATTAACTTTTTTGGTAATTTAGAAT
CAAGAGATATATTAGCTGGTAAAGTTGACATTATAGTTACTGATGGTTATACAGGAAATATGTGTTTAAAAGCTTTCGAA
GGAGCTTCTAAAATTTTAATGACTGAGATTAAATCACAATTGTATAAAACAATTTTTACAAAATTAAAAGCCTTAACACT
TAAAAAATCATTTGATAATGTTTCAAAAAAATTTGATTACAAAAATCACTCTGGTGCTATTCTACTAGGAGTTGAAGGGA
TTGCTTTTAAAGCTCATGGTTCAAGTGATGTTAAATCATTTGAAGCAACACTAAGAATGACTTGTGATGCAGTGGAAAAT
GACGTATTAAATAAAATTAAAAAGGAATTGAATTAA

Upstream 100 bases:

>100_bases
ACAAAAAGTTTACAGTTTCATAATCGCAGTAGAATAATTTATTAAAACACCAATTTGGTGTTTTAATTTTATAAAATAAT
TAAAAAGGTAGGTACTACAA

Downstream 100 bases:

>100_bases
TATGACAATGCATGAATTTTTTGAAAATTTTGGAATAAAAATTAATGATTCAAAAATATTTAGTACTGCTTTAACTCATA
ATTCATATGCTAATGAAACT

Product: putative glycerol-3-phosphate acyltransferase PlsX

Products: NA

Alternate protein names: Acyl-ACP phosphotransacylase; Acyl-[acyl-carrier-protein]--phosphate acyltransferase; Phosphate-acyl-ACP acyltransferase

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MYKIAFDVMGSDNGSAVAIEAASRFIKSRKDLYLVFVGDEDQIKTSLEKFPIDESRFEILATKEFIDMNGSIMDIRRKKD
SSMVRALETLKDKKVDAMITGGNSAAFIAGSHFILGELNGISRPGFMPTLPTAVSNKLTLLLDVGANLEADIEDIIGYAK
MANIYAKNVLKIENPLIAQLNIGEEKSKGTLLQKEIYKELESDENINFFGNLESRDILAGKVDIIVTDGYTGNMCLKAFE
GASKILMTEIKSQLYKTIFTKLKALTLKKSFDNVSKKFDYKNHSGAILLGVEGIAFKAHGSSDVKSFEATLRMTCDAVEN
DVLNKIKKELN

Sequences:

>Translated_331_residues
MYKIAFDVMGSDNGSAVAIEAASRFIKSRKDLYLVFVGDEDQIKTSLEKFPIDESRFEILATKEFIDMNGSIMDIRRKKD
SSMVRALETLKDKKVDAMITGGNSAAFIAGSHFILGELNGISRPGFMPTLPTAVSNKLTLLLDVGANLEADIEDIIGYAK
MANIYAKNVLKIENPLIAQLNIGEEKSKGTLLQKEIYKELESDENINFFGNLESRDILAGKVDIIVTDGYTGNMCLKAFE
GASKILMTEIKSQLYKTIFTKLKALTLKKSFDNVSKKFDYKNHSGAILLGVEGIAFKAHGSSDVKSFEATLRMTCDAVEN
DVLNKIKKELN
>Mature_331_residues
MYKIAFDVMGSDNGSAVAIEAASRFIKSRKDLYLVFVGDEDQIKTSLEKFPIDESRFEILATKEFIDMNGSIMDIRRKKD
SSMVRALETLKDKKVDAMITGGNSAAFIAGSHFILGELNGISRPGFMPTLPTAVSNKLTLLLDVGANLEADIEDIIGYAK
MANIYAKNVLKIENPLIAQLNIGEEKSKGTLLQKEIYKELESDENINFFGNLESRDILAGKVDIIVTDGYTGNMCLKAFE
GASKILMTEIKSQLYKTIFTKLKALTLKKSFDNVSKKFDYKNHSGAILLGVEGIAFKAHGSSDVKSFEATLRMTCDAVEN
DVLNKIKKELN

Specific function: Catalyzes the reversible formation of acyl-phosphate (acyl-PO(4)) from acyl-[acyl-carrier-protein] (acyl-ACP). This enzyme utilizes acyl-ACP as fatty acyl donor, but not acyl-CoA

COG id: COG0416

COG function: function code I; Fatty acid/phospholipid biosynthesis enzyme

Gene ontology:

Cell location: Cytoplasm. Note=Associated with the membrane possibly through plsY (By similarity)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the plsX family

Homologues:

Organism=Escherichia coli, GI87081831, Length=332, Percent_Identity=32.8313253012048, Blast_Score=190, Evalue=1e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PLSX_MESFL (Q6F1N6)

Other databases:

- EMBL:   AE017263
- RefSeq:   YP_053471.1
- ProteinModelPortal:   Q6F1N6
- SMR:   Q6F1N6
- GeneID:   2897976
- GenomeReviews:   AE017263_GR
- KEGG:   mfl:Mfl230
- HOGENOM:   HBG288268
- OMA:   EGFSGNI
- ProtClustDB:   PRK05331
- BioCyc:   MFLO265311:MFL230-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00019
- InterPro:   IPR003664
- InterPro:   IPR012281
- PIRSF:   PIRSF002465
- TIGRFAMs:   TIGR00182

Pfam domain/function: PF02504 FA_synthesis

EC number: NA

Molecular weight: Translated: 36586; Mature: 36586

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYKIAFDVMGSDNGSAVAIEAASRFIKSRKDLYLVFVGDEDQIKTSLEKFPIDESRFEIL
CEEEEEEEECCCCCCEEEEHHHHHHHHCCCCEEEEEECCCHHHHHHHHHCCCCCCCEEEE
ATKEFIDMNGSIMDIRRKKDSSMVRALETLKDKKVDAMITGGNSAAFIAGSHFILGELNG
EEHHHHCCCCCEEEEHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEEECCEEEEEECCC
ISRPGFMPTLPTAVSNKLTLLLDVGANLEADIEDIIGYAKMANIYAKNVLKIENPLIAQL
CCCCCCCCCCCHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHEEEECCCEEEEE
NIGEEKSKGTLLQKEIYKELESDENINFFGNLESRDILAGKVDIIVTDGYTGNMCLKAFE
ECCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCEEECEEEEEEECCCCCCHHHHHHC
GASKILMTEIKSQLYKTIFTKLKALTLKKSFDNVSKKFDYKNHSGAILLGVEGIAFKAHG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCEEEEECC
SSDVKSFEATLRMTCDAVENDVLNKIKKELN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MYKIAFDVMGSDNGSAVAIEAASRFIKSRKDLYLVFVGDEDQIKTSLEKFPIDESRFEIL
CEEEEEEEECCCCCCEEEEHHHHHHHHCCCCEEEEEECCCHHHHHHHHHCCCCCCCEEEE
ATKEFIDMNGSIMDIRRKKDSSMVRALETLKDKKVDAMITGGNSAAFIAGSHFILGELNG
EEHHHHCCCCCEEEEHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEEECCEEEEEECCC
ISRPGFMPTLPTAVSNKLTLLLDVGANLEADIEDIIGYAKMANIYAKNVLKIENPLIAQL
CCCCCCCCCCCHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHEEEECCCEEEEE
NIGEEKSKGTLLQKEIYKELESDENINFFGNLESRDILAGKVDIIVTDGYTGNMCLKAFE
ECCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCEEECEEEEEEECCCCCCHHHHHHC
GASKILMTEIKSQLYKTIFTKLKALTLKKSFDNVSKKFDYKNHSGAILLGVEGIAFKAHG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCEEEEECC
SSDVKSFEATLRMTCDAVENDVLNKIKKELN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA