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The map label for this gene is smpB [H]

Identifier: 50365026

GI number: 50365026

Start: 236358

End: 236807

Strand: Direct

Name: smpB [H]

Synonym: Mfl210

Alternate gene names: 50365026

Gene position: 236358-236807 (Clockwise)

Preceding gene: 50365025

Following gene: 50365027

Centisome position: 29.8

GC content: 23.78

Gene sequence:

>450_bases
ATGGGTGAACATGTTATTGCATTAAACAAAAAAGCAAAATTTAATTATGAAATACTTGAAACTTGAGAAGCAGGAATTGA
ACTTTACGGACCTGAAATTAAATCTATTAGAAATCATGAAGCAAATATTGCTGAAGCATTTATATTAATCAGAAAAAAAG
AAGCATTTTTAATAAATGCAAATATTAAAAAATATGATTATGCAAATTTTGTAAAAGGTATAGATCCTTTACGAACAAGA
AAATTGTTACTACATAAAAAGGAAATTAATAAAATTTTAAAAAGAGTAATGCTAGAAAAACTTACAATTGTACCTTTAAG
ACTTTATTTAAAAGGCAATTATGCTAAATTAGAAATTGGTTTAGGTCGTGGTAAGAAAATACATGATAAGAGAGAAACAA
TTAAAAAAAGAGATATTGAAAGAAAAGAAATGCGAAAATATAAATATTAG

Upstream 100 bases:

>100_bases
AGAATGGGACAAAAAGTTAAAATTAAAGTTATAAGCGCTGATATTAGAAAAAGAAAAATTGACTTTATTTTAGTTAAATA
AAAGTAAGGAGGTTCTTGTT

Downstream 100 bases:

>100_bases
GAGAACAATATGAATAGACAACAAACTATTGGATTAATTATTTTATTAATAGGTTTAGCATTTTTTATAGGTTTTGGTTT
AATAGCATTGTTTTATAGAA

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 149; Mature: 148

Protein sequence:

>149_residues
MGEHVIALNKKAKFNYEILETWEAGIELYGPEIKSIRNHEANIAEAFILIRKKEAFLINANIKKYDYANFVKGIDPLRTR
KLLLHKKEINKILKRVMLEKLTIVPLRLYLKGNYAKLEIGLGRGKKIHDKRETIKKRDIERKEMRKYKY

Sequences:

>Translated_149_residues
MGEHVIALNKKAKFNYEILET*EAGIELYGPEIKSIRNHEANIAEAFILIRKKEAFLINANIKKYDYANFVKGIDPLRTR
KLLLHKKEINKILKRVMLEKLTIVPLRLYLKGNYAKLEIGLGRGKKIHDKRETIKKRDIERKEMRKYKY
>Mature_148_residues
GEHVIALNKKAKFNYEILET*EAGIELYGPEIKSIRNHEANIAEAFILIRKKEAFLINANIKKYDYANFVKGIDPLRTRK
LLLHKKEINKILKRVMLEKLTIVPLRLYLKGNYAKLEIGLGRGKKIHDKRETIKKRDIERKEMRKYKY

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes [H]

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family [H]

Homologues:

Organism=Escherichia coli, GI1788973, Length=144, Percent_Identity=45.1388888888889, Blast_Score=121, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000037
- InterPro:   IPR020081
- ProDom:   PD004488 [H]

Pfam domain/function: PF01668 SmpB [H]

EC number: NA

Molecular weight: Translated: 17490; Mature: 17359

Theoretical pI: Translated: 10.61; Mature: 10.61

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGEHVIALNKKAKFNYEILETEAGIELYGPEIKSIRNHEANIAEAFILIRKKEAFLINAN
CCCEEEEECCCCCCCEEEEECCCCCEEECCHHHHHHCCCCHHHHHHHEEEECCEEEEECC
IKKYDYANFVKGIDPLRTRKLLLHKKEINKILKRVMLEKLTIVPLRLYLKGNYAKLEIGL
CCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCEEEEEEEC
GRGKKIHDKRETIKKRDIERKEMRKYKY
CCCCCCHHHHHHHHHCCCCHHHHHHCCC
>Mature Secondary Structure 
GEHVIALNKKAKFNYEILETEAGIELYGPEIKSIRNHEANIAEAFILIRKKEAFLINAN
CCEEEEECCCCCCCEEEEECCCCCEEECCHHHHHHCCCCHHHHHHHEEEECCEEEEECC
IKKYDYANFVKGIDPLRTRKLLLHKKEINKILKRVMLEKLTIVPLRLYLKGNYAKLEIGL
CCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCEEEEEEEC
GRGKKIHDKRETIKKRDIERKEMRKYKY
CCCCCCHHHHHHHHHCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA